##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937568_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1339876 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11837662589672 33.0 31.0 34.0 31.0 34.0 2 32.25640507031994 33.0 31.0 34.0 31.0 34.0 3 32.306613447811586 34.0 31.0 34.0 31.0 34.0 4 35.88978905510659 37.0 35.0 37.0 35.0 37.0 5 35.89617322797035 37.0 35.0 37.0 35.0 37.0 6 35.96941284118829 37.0 36.0 37.0 35.0 37.0 7 35.90527257746239 37.0 36.0 37.0 35.0 37.0 8 35.91324122530742 37.0 36.0 37.0 35.0 37.0 9 37.549816550188226 39.0 38.0 39.0 35.0 39.0 10-11 37.48678721015975 39.0 37.0 39.0 35.0 39.0 12-13 37.39346252936839 39.0 37.0 39.0 35.0 39.0 14-15 38.60251582982306 40.0 38.0 41.0 35.0 41.0 16-17 38.50614646429968 40.0 38.0 41.0 35.0 41.0 18-19 38.43517235923324 40.0 38.0 41.0 34.0 41.0 20-21 38.4436317241297 40.0 38.0 41.0 34.5 41.0 22-23 38.45288668503653 40.0 38.0 41.0 34.0 41.0 24-25 38.385346106654644 40.0 38.0 41.0 34.0 41.0 26-27 38.28449013192265 40.0 38.0 41.0 34.0 41.0 28-29 38.1363812770734 40.0 38.0 41.0 34.0 41.0 30-31 37.88856021004929 40.0 38.0 41.0 33.5 41.0 32-33 37.74622614331476 40.0 37.0 41.0 33.0 41.0 34-35 37.720861482704365 40.0 37.5 41.0 33.0 41.0 36-37 37.55781243936006 40.0 37.0 41.0 33.0 41.0 38-39 37.388878149918355 40.0 37.0 41.0 33.0 41.0 40-41 37.15387841859993 40.0 37.0 41.0 32.5 41.0 42-43 36.87846524603769 40.0 36.0 41.0 31.0 41.0 44-45 36.615789819356415 39.0 36.0 41.0 31.0 41.0 46-47 36.23116840662867 39.0 36.0 41.0 30.0 41.0 48-49 35.85750099262917 39.0 35.0 40.0 29.0 41.0 50-51 34.9943173845938 38.0 34.5 39.5 27.0 40.5 52-53 35.10630909128904 38.0 35.0 40.0 28.0 40.5 54-55 35.3613569464637 39.0 35.0 40.0 28.0 41.0 56-57 35.11869419259693 38.5 35.0 40.0 26.5 41.0 58-59 34.822568655606936 38.0 34.5 40.0 26.0 41.0 60-61 34.46696000226886 38.0 34.0 40.0 25.5 41.0 62-63 34.08731404995686 37.5 34.0 40.0 24.5 41.0 64-65 33.64481937134481 37.0 33.5 39.5 22.5 41.0 66-67 33.13916399726541 36.0 33.0 39.0 21.5 40.5 68-69 32.65752838322352 36.0 32.0 39.0 20.0 40.0 70-71 32.177981395293294 35.0 31.5 38.5 18.5 40.0 72-73 31.6781537993068 35.0 31.0 37.5 15.5 40.0 74-75 31.115930877185647 35.0 31.0 37.0 10.0 39.0 76-77 30.556210798611215 34.5 30.0 36.5 7.0 39.0 78-79 30.013137409730454 34.0 29.5 36.0 2.0 38.5 80-81 29.436085876603507 34.0 29.0 35.5 2.0 37.0 82-83 28.870140595099844 34.0 29.0 35.0 2.0 37.0 84-85 28.303586302016008 34.0 27.5 35.0 2.0 36.0 86-87 27.696154345626013 33.0 26.0 35.0 2.0 36.0 88-89 27.12615943564927 33.0 25.5 35.0 2.0 35.5 90-91 26.521346751490437 32.5 25.0 35.0 2.0 35.0 92-93 25.822669038030384 32.0 23.5 34.0 2.0 35.0 94-95 25.00450862617138 31.5 19.0 34.0 2.0 35.0 96-97 24.066235606877058 31.0 8.5 34.0 2.0 35.0 98-99 22.83457536369037 31.0 2.0 34.0 2.0 35.0 100-101 19.529452352307228 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.007877217824486138 1101 2 0.05516095680694377 1101 3 0.05422983353795985 1101 4 -0.005513829589645525 1101 5 -0.03696039248346494 1101 6 -0.018490937912567063 1101 7 -0.07753873436743675 1101 8 0.019376691460344375 1101 9 0.1506880141918998 1101 10-11 0.20669973833465605 1101 12-13 0.19927491781915307 1101 14-15 0.26427743555085215 1101 16-17 0.3152841427037316 1101 18-19 0.26811043304335413 1101 20-21 0.295257777545757 1101 22-23 0.22794842223900957 1101 24-25 0.21218313932200772 1101 26-27 0.2657077240431036 1101 28-29 0.24887649537264167 1101 30-31 0.20911307431857296 1101 32-33 0.2448682464408236 1101 34-35 0.4487268812745455 1101 36-37 0.5066320699781599 1101 38-39 0.41090318434280704 1101 40-41 0.4444182833004362 1101 42-43 0.3970339240168528 1101 44-45 0.5697466328215128 1101 46-47 0.7122236026260182 1101 48-49 0.9335160017517481 1101 50-51 0.5564356794904342 1101 52-53 0.7153692151272679 1101 54-55 0.7834965306650687 1101 56-57 0.8520193226213024 1101 58-59 0.7635353020700038 1101 60-61 0.9292175802795768 1101 62-63 0.9873965598807644 1101 64-65 0.9778152258251609 1101 66-67 1.012643549115701 1101 68-69 1.056694048472302 1101 70-71 1.1135451499919924 1101 72-73 1.0974436089560378 1101 74-75 1.3066547961317845 1101 76-77 1.219059471368773 1101 78-79 1.6429782822860552 1101 80-81 1.825263644578122 1101 82-83 1.8446008857765435 1101 84-85 1.746680434808173 1101 86-87 2.108228106708495 1101 88-89 2.039397381159567 1101 90-91 1.9947784846287604 1101 92-93 2.1156363812193355 1101 94-95 2.5409203029167315 1101 96-97 2.6973910190281494 1101 98-99 3.051902799230785 1101 100-101 3.792000574239008 1102 1 0.10725480988200076 1102 2 0.10761551185071738 1102 3 0.050592830619635265 1102 4 0.06703003314373746 1102 5 0.033648235905133106 1102 6 0.11890050401985519 1102 7 0.030460984348501086 1102 8 0.09333365499889368 1102 9 0.1807003203696027 1102 10-11 0.19850065953995255 1102 12-13 0.1304127118488978 1102 14-15 0.3491840844028573 1102 16-17 0.4568705848119521 1102 18-19 0.32546653866552333 1102 20-21 0.41476254179454486 1102 22-23 0.3453664103547496 1102 24-25 0.31129533650213403 1102 26-27 0.33089249251116115 1102 28-29 0.30664345152749206 1102 30-31 0.46314932480203197 1102 32-33 0.4619416615809371 1102 34-35 0.566994171454219 1102 36-37 0.36892312866104504 1102 38-39 0.41616161407453234 1102 40-41 0.4691143207112134 1102 42-43 0.5208127995838154 1102 44-45 0.5011354728763635 1102 46-47 0.4822533132550433 1102 48-49 0.7169815971949447 1102 50-51 0.6105012889978312 1102 52-53 0.5083601437162741 1102 54-55 0.701835196675404 1102 56-57 0.5786850516744266 1102 58-59 0.6272730398429402 1102 60-61 0.7384553004722605 1102 62-63 0.7582027903227342 1102 64-65 0.8956894215285445 1102 66-67 0.9036385914841176 1102 68-69 0.940335973861707 1102 70-71 0.9329263250561652 1102 72-73 0.924030930076956 1102 74-75 0.8452093476595088 1102 76-77 0.9421411140676916 1102 78-79 1.2002357626840698 1102 80-81 1.3413676845204563 1102 82-83 1.504704433471769 1102 84-85 1.4562542894546304 1102 86-87 1.6665189544283372 1102 88-89 1.6587901591912804 1102 90-91 1.81449069587309 1102 92-93 1.9962225068829902 1102 94-95 2.0183507730127204 1102 96-97 1.8271016480496378 1102 98-99 2.3770677020119813 1102 100-101 2.9238266352073268 1103 1 -0.06324840025161649 1103 2 -0.09727430248209856 1103 3 -0.017441179444567467 1103 4 -0.03813394395152869 1103 5 -0.061569532618207745 1103 6 -0.1546115556712735 1103 7 -0.02577014527148691 1103 8 1.4781429093346787E-4 1103 9 -0.017137611263230212 1103 10-11 0.06391277128731332 1103 12-13 0.11804588901357249 1103 14-15 0.13598089162132254 1103 16-17 0.14634134656798636 1103 18-19 0.1609350446519855 1103 20-21 0.14962198999860732 1103 22-23 0.09038549763567971 1103 24-25 -0.04099513720947101 1103 26-27 -0.016627896175286594 1103 28-29 0.11938854956670752 1103 30-31 0.048203116230105536 1103 32-33 -0.005966545949867452 1103 34-35 -0.008681691811446512 1103 36-37 0.04055942919896438 1103 38-39 0.11901342736621956 1103 40-41 0.31472598130465457 1103 42-43 0.0070378559780621686 1103 44-45 0.03578929900859151 1103 46-47 0.02395988969092855 1103 48-49 0.03352940215764022 1103 50-51 -0.11386796312780234 1103 52-53 0.04036344061024977 1103 54-55 0.006917619024868316 1103 56-57 0.06764913288213137 1103 58-59 0.032600994033465724 1103 60-61 0.2937754462296809 1103 62-63 0.3853240813170018 1103 64-65 0.2543255485457294 1103 66-67 0.38892923871646445 1103 68-69 0.5796418909188006 1103 70-71 0.48585709028260027 1103 72-73 0.47277116953557297 1103 74-75 0.45284599523889213 1103 76-77 0.541014096889139 1103 78-79 0.7017662155731905 1103 80-81 0.891958522625611 1103 82-83 0.9564630661283715 1103 84-85 1.0985117942264502 1103 86-87 1.3455836776627628 1103 88-89 1.4682904194723818 1103 90-91 1.5146459969315664 1103 92-93 1.4502134838047347 1103 94-95 1.4918194525199233 1103 96-97 1.775860974789179 1103 98-99 2.160679536126157 1103 100-101 2.8635463992191283 1104 1 0.08692958363765513 1104 2 -0.032814352402674274 1104 3 0.018809742384341632 1104 4 -0.015194231562148275 1104 5 0.028337149932283978 1104 6 0.05811854700628061 1104 7 0.07418411783266521 1104 8 0.08693942422966217 1104 9 0.22420145159131977 1104 10-11 0.22211208487932055 1104 12-13 0.15431613379459463 1104 14-15 0.287145764267116 1104 16-17 0.25554917644091546 1104 18-19 0.22363820156144953 1104 20-21 0.3654384467719254 1104 22-23 0.2593765913502324 1104 24-25 0.21078275166050986 1104 26-27 0.25052060337088733 1104 28-29 0.24993742777184025 1104 30-31 0.18400056914591545 1104 32-33 0.08630959144519323 1104 34-35 0.3184074915899586 1104 36-37 0.2952148424619523 1104 38-39 0.47301469665371343 1104 40-41 0.34366041799627567 1104 42-43 0.2644369511675322 1104 44-45 0.221817740975915 1104 46-47 0.155923420721102 1104 48-49 0.13696321484200524 1104 50-51 0.10082439420145306 1104 52-53 0.15665836204206585 1104 54-55 0.31411036635866196 1104 56-57 0.40784229149343076 1104 58-59 0.3905677231008582 1104 60-61 0.5329427156306323 1104 62-63 0.5977990855716016 1104 64-65 0.6863716896280962 1104 66-67 0.8212990835655631 1104 68-69 0.6283704761241538 1104 70-71 0.7061036440606827 1104 72-73 0.6420753305294475 1104 74-75 0.7643786067092826 1104 76-77 0.8496206106740232 1104 78-79 1.1385035794261533 1104 80-81 1.3734653937964758 1104 82-83 1.2993933474989205 1104 84-85 1.6125106525768018 1104 86-87 1.8280609510346721 1104 88-89 1.7112788469740856 1104 90-91 1.844836425511101 1104 92-93 1.886069791950863 1104 94-95 2.2719636123792384 1104 96-97 2.2568845948822123 1104 98-99 2.3079564180753316 1104 100-101 3.103478077515021 1105 1 0.13641447374754279 1105 2 0.18203275700965094 1105 3 0.075612925614152 1105 4 0.05364311315835124 1105 5 0.09508046096907208 1105 6 0.16433345981086944 1105 7 0.12138535409640383 1105 8 0.15165663636535243 1105 9 0.30552311905429264 1105 10-11 0.23621713743546024 1105 12-13 0.26779670892598517 1105 14-15 0.2800353814434118 1105 16-17 0.32472955448677965 1105 18-19 0.4382889719866725 1105 20-21 0.43032885527646414 1105 22-23 0.30905110162255767 1105 24-25 0.32285909243929467 1105 26-27 0.36483841870772693 1105 28-29 0.27642788704082477 1105 30-31 0.33767800583965624 1105 32-33 0.3999047598637233 1105 34-35 0.46241616633913196 1105 36-37 0.4870122773640091 1105 38-39 0.45436585229156634 1105 40-41 0.3005501994105231 1105 42-43 0.33834476889328613 1105 44-45 0.5100101531832664 1105 46-47 0.6207425350307076 1105 48-49 0.5094496278854805 1105 50-51 0.2825473248034598 1105 52-53 0.4440251053531057 1105 54-55 0.5444219705153799 1105 56-57 0.6838366775421676 1105 58-59 0.7291711034161992 1105 60-61 0.752627752061592 1105 62-63 0.8734019134502446 1105 64-65 0.7820175179845208 1105 66-67 1.042904210775312 1105 68-69 1.0690462405770873 1105 70-71 0.8490242424839991 1105 72-73 0.995810250500778 1105 74-75 1.0284355076887373 1105 76-77 1.0468707450505192 1105 78-79 1.2907290034586403 1105 80-81 1.4783406476187402 1105 82-83 1.5234347951816716 1105 84-85 1.9526993022423547 1105 86-87 1.992770324541599 1105 88-89 1.9425497499841171 1105 90-91 1.9438312146893146 1105 92-93 2.1765111440770006 1105 94-95 2.2506671778355205 1105 96-97 2.4725346038406464 1105 98-99 3.0444571795421247 1105 100-101 3.295783156946456 1106 1 -0.04016722440026399 1106 2 -0.09673999671984745 1106 3 -0.06700741101745677 1106 4 -0.09162435030412297 1106 5 -0.11795185470877811 1106 6 -0.1466473325730604 1106 7 -0.11257760207451639 1106 8 -0.0906072949369161 1106 9 0.09410060731364922 1106 10-11 -0.28846452961658287 1106 12-13 -0.4462330632775888 1106 14-15 -0.550543318753526 1106 16-17 -0.3408550060662421 1106 18-19 -0.07326071346768259 1106 20-21 -0.15676241478628583 1106 22-23 -0.09903100714883806 1106 24-25 -0.44342791351674293 1106 26-27 -0.2330590290474035 1106 28-29 -0.19000383383081498 1106 30-31 -0.058789435297647685 1106 32-33 0.09668794263507863 1106 34-35 0.017838391041927082 1106 36-37 -0.2327275547188492 1106 38-39 -0.24790732800807547 1106 40-41 -0.14843759350762298 1106 42-43 0.10258543418323285 1106 44-45 0.04623552573448109 1106 46-47 0.09211928018096671 1106 48-49 0.24803309610002344 1106 50-51 0.3008176825642437 1106 52-53 0.4428753593018655 1106 54-55 0.45553073394037114 1106 56-57 0.15349438490707712 1106 58-59 -0.015103687484703698 1106 60-61 0.3634881480143548 1106 62-63 0.370375258796642 1106 64-65 0.4196469840143848 1106 66-67 0.5082093080891141 1106 68-69 0.46248503374239647 1106 70-71 0.3617974267702735 1106 72-73 0.4277264673419232 1106 74-75 0.5690302341655844 1106 76-77 0.6493603997280388 1106 78-79 0.7617716684456077 1106 80-81 0.6541672767578675 1106 82-83 0.739593431432958 1106 84-85 0.7314736506265724 1106 86-87 0.9269924121040916 1106 88-89 1.0032211793528774 1106 90-91 1.4057460760739495 1106 92-93 1.352203024892507 1106 94-95 1.4974491646158334 1106 96-97 1.4560339141524814 1106 98-99 1.6974184533523093 1106 100-101 2.2029858136412166 1107 1 0.12239298352383088 1107 2 0.14442409965081993 1107 3 0.20240830896880624 1107 4 0.22304366917767737 1107 5 0.19755191328237487 1107 6 0.1794394174890641 1107 7 0.15754882071609444 1107 8 0.1264902517929798 1107 9 0.14936210320851018 1107 10-11 0.2491338647332526 1107 12-13 0.28768550505989765 1107 14-15 0.4509603954204877 1107 16-17 0.39465472929588685 1107 18-19 0.47556409433389746 1107 20-21 0.3982727325539628 1107 22-23 0.33173799227975564 1107 24-25 0.3834502650580234 1107 26-27 0.49246208705674377 1107 28-29 0.44832402017911477 1107 30-31 0.33244177421089205 1107 32-33 0.4872013264826549 1107 34-35 0.302924395578394 1107 36-37 0.2875464694893637 1107 38-39 0.45334265418478736 1107 40-41 0.4397453499416457 1107 42-43 0.2996432195446559 1107 44-45 0.28548594796158966 1107 46-47 0.4318866635125289 1107 48-49 0.4165852092933022 1107 50-51 0.29609206291529233 1107 52-53 0.3464188221814908 1107 54-55 0.3595352039286084 1107 56-57 0.34926966036377394 1107 58-59 0.15697819687492398 1107 60-61 0.4693278615805454 1107 62-63 0.510632615520386 1107 64-65 0.5453543232475582 1107 66-67 0.5855469109154612 1107 68-69 0.7519432850969352 1107 70-71 0.928546111811805 1107 72-73 0.8995484465780059 1107 74-75 0.7837594310714167 1107 76-77 0.7994773728622064 1107 78-79 0.9408450322858712 1107 80-81 1.1614804719288863 1107 82-83 1.3926216743508562 1107 84-85 1.4733769412280395 1107 86-87 1.633738073888697 1107 88-89 1.3292006908515397 1107 90-91 1.6226944385292157 1107 92-93 1.8011111701164744 1107 94-95 2.0201133029322094 1107 96-97 2.1468267514078683 1107 98-99 2.432964198711769 1107 100-101 2.929603453949621 1108 1 0.04446543606795217 1108 2 0.020797042527505027 1108 3 0.09173135054373205 1108 4 -0.009516456419831343 1108 5 0.0017823282517497319 1108 6 0.05403649389968024 1108 7 0.05296572818151901 1108 8 0.06372581186163728 1108 9 0.13340114263399272 1108 10-11 0.14112558047607138 1108 12-13 0.05445445757945322 1108 14-15 0.10817839180323574 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100-101 -1.9442983647926724 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4775.0 3 778.0 4 787.0 5 857.0 6 880.0 7 1099.0 8 1293.0 9 1634.0 10 2080.0 11 2874.0 12 3835.0 13 5233.0 14 6315.0 15 8108.0 16 10510.0 17 13974.0 18 12375.0 19 8466.0 20 8118.0 21 8560.0 22 9189.0 23 10354.0 24 11829.0 25 13414.0 26 15857.0 27 18415.0 28 22341.0 29 27299.0 30 33462.0 31 42461.0 32 55123.0 33 73819.0 34 102872.0 35 147703.0 36 209829.0 37 263414.0 38 166698.0 39 13244.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.924316391625986 19.128006229596572 15.210323759321934 22.737353619455508 2 14.987902359732107 19.7019782055379 41.540071141458945 23.77004829327105 3 19.168659324704095 24.323974389198856 29.84182224367682 26.66554404242023 4 12.442010400896253 16.4884664403086 37.707477225120506 33.362045933674636 5 13.653293938829256 37.247563475367585 34.219559999910196 14.879582585892967 6 31.74102705811351 36.08359215321439 17.623334248208327 14.552046540463776 7 28.393006700167504 32.83934553720986 20.437679468772433 18.329968293850204 8 26.190162806524224 35.09082614775983 19.571183918275164 19.147827127440788 9 25.990456084302345 17.070462096924363 20.67960576942492 36.25947604934837 10-11 24.728757450172328 26.87783231816419 27.1592178342701 21.23419239739338 12-13 25.669684425447898 24.155494976011248 28.010597680775717 22.164222917765137 14-15 22.502251032782816 25.406980744321565 26.69803404817958 25.392734174716043 16-17 21.657955455982243 28.95495757561255 27.086063570962 22.301023397443206 18-19 21.513161018574564 27.100223989495564 30.00128239687828 21.385332595051594 20-21 22.215351369943676 26.19692718371284 30.27656842054102 21.311153025802458 22-23 21.852777596021724 26.19051981192922 30.20622353502024 21.75047905702882 24-25 21.60961545723786 26.312877005915436 30.4772395268397 21.600268010007003 26-27 21.48104524574717 26.663750119185835 30.070614101507072 21.78459053355993 28-29 21.307624569282225 26.695845478187874 30.315276996165373 21.681252956364528 30-31 21.493932707290288 26.39402271359858 30.353515013702868 21.75852956540826 32-33 21.253318166707984 26.53318091921865 30.271626930930907 21.94187398314246 34-35 21.78579534852763 26.546231097340495 30.014624425969778 21.6533491281621 36-37 21.703036931101202 26.381275529757065 30.007085237841522 21.908602301300206 38-39 21.460790475147217 26.655478169906882 29.99580150973672 21.88792984520918 40-41 21.752016817184153 26.546731202822706 30.006770389583394 21.694481590409747 42-43 21.620781901081827 26.503055557113402 29.851400284524804 22.024762257279964 44-45 21.564657744437817 26.870512527162248 29.63818313151389 21.926646596886044 46-47 21.697664879796626 26.996768378794012 29.184497042260844 22.121069699148517 48-49 21.916427224197424 27.16519062412593 28.858214659114196 22.060167492562453 50-51 21.88078291482277 27.262097731611057 28.763762612807152 22.09335674075902 52-53 21.98780368776334 27.304795992052615 28.698318341923805 22.009081978260244 54-55 21.87953256039955 27.21355340022188 28.73856892707483 22.168345112303736 56-57 21.891474015773934 27.57092775227043 28.586798133564805 21.950800098390832 58-59 22.034694620509836 27.18712085930708 28.653038096654853 22.125146423528232 60-61 21.92654044469898 27.15744873969308 28.960050063120953 21.955960752486988 62-63 21.806103754785205 27.235135821122597 29.006423135999054 21.952337288093144 64-65 21.76515580291755 27.180108127830394 28.885502780953292 22.169233288298763 66-67 21.750962886037584 27.448728631556047 28.64329422724447 22.157014255161904 68-69 21.747500881294243 27.2898790785793 28.739014662568042 22.223605377558417 70-71 21.761433538579208 27.351221662181313 28.64493983745065 22.24240496178883 72-73 21.886041767064068 27.254880482072032 28.643963052011244 22.215114698852656 74-75 21.785716021590886 27.437041224505514 28.518978392288773 22.25826436161482 76-77 21.876955863383994 27.37532013534481 28.394743237432934 22.35298076383826 78-79 21.870694673386822 27.426339541914185 28.39159260024997 22.311373184449025 80-81 21.68785416609808 27.595990532266523 28.44436285899829 22.271792442637103 82-83 21.860581786480907 27.611082159938572 28.409287170626413 22.119048882954107 84-85 21.864180335283912 27.46579014065237 28.299439349319243 22.37059017474448 86-87 21.901564063657116 27.383655966555754 28.44062042176149 22.27415954802564 88-89 22.029955047240772 27.37131038938122 28.251224013088045 22.347510550289964 90-91 21.851734989827605 27.436458974326385 28.349843694273325 22.36196234157269 92-93 21.958325077850755 27.34541063704911 28.33700312898366 22.35926115611647 94-95 21.837265847316075 27.30328333390655 28.22408077267582 22.635370046101556 96-97 21.918580524078525 27.249017679892628 28.192643160771503 22.639758635257348 98-99 21.873301265970436 27.209802952460493 28.024709266460945 22.892186515108122 100-101 22.15887618853245 26.559207671199697 28.667569011229965 22.61434712903789 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1852.0 1 1108.5 2 468.0 3 537.5 4 385.5 5 321.5 6 614.5 7 1413.0 8 2412.5 9 2737.5 10 2254.5 11 1697.5 12 1558.5 13 1694.5 14 1785.0 15 1871.5 16 2043.5 17 2155.0 18 2259.5 19 2459.5 20 2689.0 21 3140.5 22 3898.0 23 4850.0 24 5850.0 25 7143.0 26 9048.5 27 10967.0 28 12642.5 29 14336.0 30 16553.5 31 19244.5 32 22021.0 33 24629.5 34 27216.5 35 29738.0 36 32622.0 37 35215.0 38 36974.5 39 38455.5 40 40267.5 41 42883.5 42 45002.5 43 47219.5 44 49238.0 45 50824.0 46 51342.5 47 51146.5 48 52193.0 49 51661.0 50 49269.5 51 46537.0 52 43667.0 53 42065.5 54 40602.5 55 38062.0 56 34830.0 57 30924.0 58 27724.5 59 24362.0 60 20297.0 61 16579.5 62 13217.0 63 10205.0 64 7681.5 65 6075.0 66 4770.0 67 3619.5 68 2582.0 69 1763.0 70 1273.5 71 927.5 72 723.0 73 609.5 74 494.0 75 389.5 76 275.5 77 187.5 78 149.5 79 94.0 80 51.5 81 39.0 82 23.0 83 14.0 84 12.0 85 9.5 86 8.5 87 8.5 88 5.5 89 5.5 90 4.5 91 4.0 92 3.0 93 2.0 94 4.5 95 4.0 96 1.5 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3221193602990127 2 0.2735327746746714 3 0.2777122659111739 4 0.2720400992330633 5 0.27233863432138494 6 0.20277995874245078 7 0.19374927232072223 8 0.20195898724956637 9 0.20069021312419957 10-11 0.20468311993050103 12-13 0.2083028578764005 14-15 0.20240678988204877 16-17 0.19512999710420964 18-19 0.18930856288193834 20-21 0.18684564840328508 22-23 0.19505536333212925 24-25 0.19498072956004883 26-27 0.19990655851735534 28-29 0.2028919094005714 30-31 0.20505628879090304 32-33 0.20371288089345582 34-35 0.20371288089345582 36-37 0.1931895190301192 38-39 0.18587540936623986 40-41 0.18169591812973737 42-43 0.19095050586770715 44-45 0.18971904862838054 46-47 0.19565243350877243 48-49 0.20464580304446084 50-51 0.20102606509856139 52-53 0.21140015941773718 54-55 0.1997572909731945 56-57 0.17561326570518465 58-59 0.16023870865662196 60-61 0.17647155408410928 62-63 0.17087402117807918 64-65 0.1780015464117575 66-67 0.17617301899578766 68-69 0.17572521636330526 70-71 0.1772178918049133 72-73 0.18527833918959663 74-75 0.19050270323522475 76-77 0.19039075257710417 78-79 0.1870695497195263 80-81 0.18595004313832025 82-83 0.1772552086909535 84-85 0.1733742525427726 86-87 0.17662082162827009 88-89 0.18449468458275245 90-91 0.1815839674716168 92-93 0.17770301132343588 94-95 0.17867325036048112 96-97 0.18516638853147604 98-99 0.18531565607563685 100-101 0.1678886702948631 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1339876.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.22811978966545 #Duplication Level Percentage of deduplicated Percentage of total 1 80.9891761689595 50.398041563083304 2 11.35869159612852 14.136600425955034 3 3.271932225590953 6.108185714332216 4 1.3978696008077525 3.479471878775865 5 0.7608010532457914 2.3671609538741376 6 0.4584951514757537 1.7118774725408408 7 0.31074977315896285 1.3536161876123114 8 0.22946685834902464 1.142343291928104 9 0.17230235689829704 0.9649846534598026 >10 0.957413799092591 11.059239542643066 >50 0.06469541714532875 2.71699752310859 >100 0.027327303033226658 2.874176235640577 >500 3.595653714420968E-4 0.13365379331771773 >1k 4.7942049525612906E-4 0.6147179489534763 >5k 2.3971024762806453E-4 0.9389328147749713 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7142 0.5330344001982272 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5447 0.4065301565219468 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3953 0.295027301033827 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1842 0.13747540817209952 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1400 0.10448728091256206 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 7.463377208040147E-5 0.0 0.0 3.7316886040200736E-5 0.0 24-25 7.463377208040147E-5 0.0 0.0 1.4926754416080294E-4 0.0 26-27 7.463377208040147E-5 0.0 0.0 1.4926754416080294E-4 0.0 28-29 1.4926754416080294E-4 0.0 0.0 5.224364045628103E-4 0.0 30-31 1.4926754416080294E-4 0.0 0.0 0.0014180416695276279 0.0 32-33 1.4926754416080294E-4 0.0 0.0 0.003134618427376862 0.0 34-35 1.4926754416080294E-4 0.0 0.0 0.005709483564150713 0.0 36-37 2.239013162412044E-4 0.0 0.0 0.009702390370452191 0.0 38-39 2.985350883216059E-4 0.0 0.0 0.01627016231352752 0.0 40-41 2.985350883216059E-4 0.0 0.0 0.02403207460988927 0.0 42-43 3.358519743618066E-4 0.0 0.0 0.031831303792291224 0.0 44-45 3.7316886040200735E-4 0.0 0.0 0.04209344745334643 0.0 46-47 3.7316886040200735E-4 0.0 0.0 0.05481850559305488 0.0 48-49 3.7316886040200735E-4 0.0 0.0 0.07026769641369798 0.0 50-51 3.7316886040200735E-4 0.0 0.0 0.08597810543662249 0.0 52-53 4.104857464422081E-4 0.0 0.0 0.10418874582424045 0.0 54-55 4.478026324824088E-4 0.0 0.0 0.12489961757655185 0.0 56-57 4.478026324824088E-4 0.0 0.0 0.14990193122348636 0.0 58-59 4.478026324824088E-4 0.0 0.0 0.18225567142034038 0.0 60-61 4.478026324824088E-4 0.0 0.0 0.21516916490779742 0.0 62-63 5.224364045628103E-4 0.0 0.0 0.24744827133257108 0.0 64-65 5.970701766432118E-4 0.0 0.0 0.2867429523329025 0.0 66-67 6.717039487236132E-4 0.0 0.0 0.33540417172932424 0.0 68-69 6.717039487236132E-4 0.0 0.0 0.3977233714164594 0.0 70-71 7.090208347638139E-4 0.0 0.0 0.46552815335150416 0.0 72-73 7.463377208040147E-4 0.0 0.0 0.540049974773785 0.0 74-75 7.463377208040147E-4 0.0 0.0 0.6171093444467995 0.0 76-77 7.463377208040147E-4 0.0 0.0 0.7026396472509396 0.0 78-79 7.463377208040147E-4 3.7316886040200736E-5 0.0 0.8102615465908785 0.0 80-81 7.463377208040147E-4 7.463377208040147E-5 0.0 0.9250109711644958 0.0 82-83 7.463377208040147E-4 7.463377208040147E-5 0.0 1.048865715931922 0.0 84-85 7.836546068442155E-4 7.463377208040147E-5 0.0 1.1791389650982627 0.0 86-87 8.209714928844162E-4 7.463377208040147E-5 0.0 1.3273243195639 0.0 88-89 8.209714928844162E-4 7.463377208040147E-5 0.0 1.4957727431493661 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3180 0.0 48.90437 1 GTATCAA 5520 0.0 42.04436 1 ATCAACG 7020 0.0 32.49908 3 TCAACGC 7140 0.0 31.95288 4 TATCAAC 7260 0.0 31.55567 2 CAACGCA 7305 0.0 31.295044 5 AACGCAG 7445 0.0 30.816942 6 ACGCAGA 8565 0.0 26.621788 7 CGCAGAG 8700 0.0 26.09949 8 GCAGAGT 9120 0.0 24.740355 9 TGGTATC 1585 0.0 24.589607 2 GTACATG 5550 0.0 23.39358 1 GAGTACT 5730 0.0 23.042646 12-13 GTGGTAT 1720 0.0 22.67318 1 TACATGG 5525 0.0 22.453043 2 TATCGTT 110 0.002876466 21.592388 7 ACATGGG 5685 0.0 21.486696 3 GTACTTT 6125 0.0 21.478287 14-15 AGAGTAC 8630 0.0 21.381523 10-11 AGTACTT 6060 0.0 21.27842 12-13 >>END_MODULE