FastQCFastQC Report
Thu 26 May 2016
SRR937568_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937568_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339876
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72390.5402738760900263No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63950.4772829724541674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44100.32913493487457046No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16940.1264296099042001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36200.051.994441
GTATCAA63800.042.6133421
TATCAAC86700.031.4522882
ATCAACG86900.031.3252353
TCAACGC89000.030.5860984
CAACGCA91550.029.6822725
AACGCAG93200.029.253276
CGTCGCA350.0083949627.14190982-83
ACGCAGA105150.025.8393387
CGCAGAG105950.025.59948
TGGTATC16450.024.5477962
GCAGAGT110750.024.2754529
GGACCGA1852.5431473E-723.10736
GAGTACT73200.022.51712812-13
GTACTTT74000.022.01612314-15
AGAGTAC104000.022.01447510-11
GTGGTAT19250.021.481211
AGTACTT76350.021.37038412-13
CAGAGTA110900.020.43061610-11
AGGACCG2902.4356268E-919.6581545
Produced by FastQC (version 0.11.5)