##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937568_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1339876 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.463618275123967 31.0 31.0 34.0 30.0 34.0 2 31.742357501738965 31.0 31.0 34.0 30.0 34.0 3 31.814194000041795 31.0 31.0 34.0 30.0 34.0 4 35.61975063364073 37.0 35.0 37.0 33.0 37.0 5 35.369446874188355 37.0 35.0 37.0 33.0 37.0 6 35.525361302090644 37.0 35.0 37.0 33.0 37.0 7 35.463780230409384 37.0 35.0 37.0 33.0 37.0 8 35.45943729121202 37.0 35.0 37.0 33.0 37.0 9 37.009508342563045 39.0 37.0 39.0 33.0 39.0 10-11 36.899166042230775 39.0 37.0 39.0 33.0 39.0 12-13 36.78469836014676 39.0 37.0 39.0 32.0 39.0 14-15 37.7371909788667 39.0 37.0 41.0 32.5 41.0 16-17 37.6730272055026 39.0 37.0 40.0 32.5 41.0 18-19 37.633600795894544 39.0 37.0 40.0 32.0 41.0 20-21 37.51977384474384 39.0 36.0 40.0 32.0 41.0 22-23 37.31726704560721 39.0 36.0 40.0 32.0 41.0 24-25 37.224974176714866 39.0 36.0 40.0 31.5 41.0 26-27 37.082746463105536 39.0 36.0 40.0 31.0 41.0 28-29 36.91650794551138 39.0 36.0 40.0 31.0 41.0 30-31 36.95742068669041 39.0 36.0 40.0 31.0 41.0 32-33 36.83014734199284 39.0 35.0 40.0 31.0 41.0 34-35 36.62634490057289 38.0 35.0 40.0 30.0 41.0 36-37 36.37967393997654 38.0 35.0 40.0 30.0 41.0 38-39 36.18311657198129 38.0 35.0 40.0 30.0 41.0 40-41 35.908747152721595 38.0 35.0 40.0 29.5 41.0 42-43 35.62204114410588 38.0 34.5 40.0 28.5 41.0 44-45 35.21009220256203 38.0 34.0 40.0 27.0 41.0 46-47 34.75130198615395 38.0 33.5 40.0 25.5 41.0 48-49 34.320600189868316 38.0 33.0 40.0 24.0 41.0 50-51 33.98409778218283 38.0 33.0 40.0 23.5 41.0 52-53 33.651227053846775 37.0 33.0 40.0 23.0 41.0 54-55 33.331714278037666 37.0 32.0 39.5 22.0 40.0 56-57 32.93599631607701 36.5 31.5 39.0 20.5 40.0 58-59 32.56660168552911 36.0 31.0 39.0 19.0 40.0 60-61 32.09179319578827 36.0 31.0 39.0 17.5 40.0 62-63 31.589673969830045 35.0 30.0 38.0 14.0 40.0 64-65 31.04656139821894 35.0 29.0 38.0 10.5 40.0 66-67 30.4691422937645 34.0 28.5 38.0 8.0 40.0 68-69 29.87659753589138 34.0 28.0 37.0 7.0 39.0 70-71 29.350642148974977 34.0 27.5 36.5 6.5 39.0 72-73 28.79009027701071 33.0 26.0 36.0 2.0 39.0 74-75 28.149318668294676 32.5 26.0 35.5 2.0 38.0 76-77 27.24883907167529 31.5 25.5 34.0 2.0 36.5 78-79 27.793012935525375 32.0 26.0 35.0 2.0 37.0 80-81 27.488688505503497 32.0 26.0 35.0 2.0 36.5 82-83 26.977518442005078 32.0 25.0 35.0 2.0 36.0 84-85 26.35814284306906 31.5 24.5 34.0 2.0 36.0 86-87 25.67143377446868 31.0 22.5 34.0 2.0 35.0 88-89 24.94723392313916 31.0 19.5 34.0 2.0 35.0 90-91 24.075362570864765 30.0 17.0 34.0 2.0 35.0 92-93 23.239988252644274 30.0 7.5 34.0 2.0 35.0 94-95 22.3467988828817 29.0 2.0 34.0 2.0 35.0 96-97 21.299036627269984 29.0 2.0 34.0 2.0 35.0 98-99 19.961859903453753 28.0 2.0 33.0 2.0 34.0 100-101 17.54558780066215 21.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07931892451636813 1101 2 0.10976442256973584 1101 3 -0.006957383550428631 1101 4 0.03680266609401883 1101 5 -0.18700504600003143 1101 6 -0.02067064395038898 1101 7 -0.04939607926517908 1101 8 0.047300303932686916 1101 9 0.059103858415141985 1101 10-11 -0.04915713371153174 1101 12-13 0.11613073091535142 1101 14-15 -0.03995640002578682 1101 16-17 -0.2045721956349098 1101 18-19 -0.06615753490976317 1101 20-21 0.08038977582811668 1101 22-23 0.012895989446100486 1101 24-25 0.07187507777194213 1101 26-27 -0.015569471871806684 1101 28-29 0.19188225718170315 1101 30-31 0.012137203550580011 1101 32-33 0.032798985137475256 1101 34-35 -0.06726573600936803 1101 36-37 -0.03470105635265952 1101 38-39 0.1645517614251233 1101 40-41 0.2359520567985811 1101 42-43 -0.011669580145749592 1101 44-45 0.09491614771081203 1101 46-47 0.01637128355731221 1101 48-49 0.1030496065851807 1101 50-51 0.41509629340716714 1101 52-53 0.22576313737369702 1101 54-55 0.30142538635966787 1101 56-57 0.2733156101826779 1101 58-59 0.33530512381435784 1101 60-61 0.43143828805362006 1101 62-63 0.3800434260221799 1101 64-65 0.23485946857480045 1101 66-67 0.6331789616975705 1101 68-69 0.1949600024898146 1101 70-71 0.20557767547606431 1101 72-73 0.46114587919543837 1101 74-75 0.4177012788329044 1101 76-77 0.2526603623564547 1101 78-79 0.2072994453113033 1101 80-81 0.3957830634483628 1101 82-83 0.49346725276519976 1101 84-85 0.5844464469002801 1101 86-87 0.9349776916467682 1101 88-89 1.1008275208358498 1101 90-91 1.0597509278406534 1101 92-93 1.37554386878357 1101 94-95 1.4813447573918204 1101 96-97 1.8341783798671862 1101 98-99 1.8578014451673468 1101 100-101 2.2529678823283774 1102 1 0.17002565072149523 1102 2 0.21059174931633606 1102 3 0.18783604846673185 1102 4 0.07919709666959562 1102 5 0.04007135778324056 1102 6 0.07639310205010474 1102 7 0.07264955907384518 1102 8 0.2271153596620934 1102 9 0.2113994879640373 1102 10-11 0.25908448929750705 1102 12-13 0.20615551907329177 1102 14-15 0.2820960946122355 1102 16-17 0.31373482591848045 1102 18-19 0.4101608083271131 1102 20-21 0.2883253266174677 1102 22-23 0.3987706070755834 1102 24-25 0.16585735687605307 1102 26-27 -0.08650456557456465 1102 28-29 -0.06441358333023572 1102 30-31 0.22570986273977667 1102 32-33 0.03431387561249011 1102 34-35 0.15908143884383463 1102 36-37 0.08988334644612195 1102 38-39 0.2827130426740254 1102 40-41 0.15989829638227349 1102 42-43 0.38468198977091816 1102 44-45 0.35854752494790176 1102 46-47 0.6573084868905283 1102 48-49 0.49509067405819707 1102 50-51 0.6268578348438254 1102 52-53 0.6078284655970378 1102 54-55 0.36474067063237925 1102 56-57 0.6002638539153224 1102 58-59 0.625999192236705 1102 60-61 0.6646595179681825 1102 62-63 0.6989198719980543 1102 64-65 0.6973570952405268 1102 66-67 0.8629790742111929 1102 68-69 0.9184412443589451 1102 70-71 0.8660182366377676 1102 72-73 0.9048296455890217 1102 74-75 0.7604759702994492 1102 76-77 0.8723477854428445 1102 78-79 1.1938749876621308 1102 80-81 1.1330758801566354 1102 82-83 1.1620351652858574 1102 84-85 1.3564254560771722 1102 86-87 1.5092038088718986 1102 88-89 1.6892430125555506 1102 90-91 1.8565149305128514 1102 92-93 2.19834668865629 1102 94-95 1.856541128827427 1102 96-97 2.418798118976216 1102 98-99 2.4951709117824485 1102 100-101 2.7929089884779543 1103 1 0.14938188879194314 1103 2 0.15189141962693498 1103 3 0.11694241667773042 1103 4 0.06170273609350119 1103 5 -0.08035897364136702 1103 6 -0.013936587347778584 1103 7 0.04702054640682718 1103 8 0.024380672867025055 1103 9 0.177160376390205 1103 10-11 0.13531214850277706 1103 12-13 0.11611821211511142 1103 14-15 0.2929784475534092 1103 16-17 0.19768242265628544 1103 18-19 0.08810458760873985 1103 20-21 0.28596804742260673 1103 22-23 0.3509864668708289 1103 24-25 0.3524702321146478 1103 26-27 0.2633123700492419 1103 28-29 0.1639796146569239 1103 30-31 0.0951945061546624 1103 32-33 0.14178307568190718 1103 34-35 0.2848457976847172 1103 36-37 0.21179641255751847 1103 38-39 0.09191397968599802 1103 40-41 0.10559237932518784 1103 42-43 0.36385863387989303 1103 44-45 0.15181144998694407 1103 46-47 0.2831483272514532 1103 48-49 0.405897545479327 1103 50-51 0.5178469030374728 1103 52-53 0.24144637293697002 1103 54-55 0.2742756350606328 1103 56-57 0.40935547286378693 1103 58-59 0.3594383732188291 1103 60-61 0.23402703488645216 1103 62-63 0.46157907727308967 1103 64-65 0.5348575290423696 1103 66-67 0.8877517620473903 1103 68-69 0.5025137760264826 1103 70-71 0.44190405999365723 1103 72-73 0.4731916098437523 1103 74-75 0.597910466221709 1103 76-77 0.6073486530465217 1103 78-79 0.7084012760535963 1103 80-81 0.5155962688430833 1103 82-83 0.6316829181923325 1103 84-85 1.1419251957960697 1103 86-87 1.2509190613445718 1103 88-89 1.2502919454030277 1103 90-91 1.4236856749168076 1103 92-93 1.8476257099993383 1103 94-95 1.8116374328357558 1103 96-97 2.1160374040707843 1103 98-99 2.1933879862228416 1103 100-101 2.352635693319179 1104 1 0.16621767560837242 1104 2 0.22887370632991022 1104 3 0.14024837878347185 1104 4 0.15257379350217093 1104 5 -0.022748212352503572 1104 6 0.07988857716322428 1104 7 0.08612523780687553 1104 8 0.13710687549919953 1104 9 0.1344438884165271 1104 10-11 0.16538398024773926 1104 12-13 0.2435261355891285 1104 14-15 0.2734471975534092 1104 16-17 0.21667741641621774 1104 18-19 0.17177492031806452 1104 20-21 0.27320018521475475 1104 22-23 0.24927682879503976 1104 24-25 0.2467008240706221 1104 26-27 0.20019368103177015 1104 28-29 0.277660087484243 1104 30-31 0.22906959124656368 1104 32-33 0.24552428285231542 1104 34-35 0.28674263793885757 1104 36-37 0.2722313385275683 1104 38-39 0.17188866484597298 1104 40-41 0.31593831335060685 1104 42-43 0.33622752144513157 1104 44-45 0.12332693671260841 1104 46-47 0.28948962376835397 1104 48-49 0.2949627046011827 1104 50-51 0.18192429411984534 1104 52-53 0.3106968196694382 1104 54-55 0.18250792176360164 1104 56-57 0.5184454150012954 1104 58-59 0.4687827046801445 1104 60-61 0.32347032113560203 1104 62-63 0.39555024530123006 1104 64-65 0.2788807094939223 1104 66-67 0.32134799389445234 1104 68-69 0.3462183936802177 1104 70-71 0.4357962343753208 1104 72-73 0.8818665516976232 1104 74-75 0.6760712757293135 1104 76-77 0.4909478985650182 1104 78-79 0.8388742806485574 1104 80-81 0.8218852692969065 1104 82-83 0.854932506914821 1104 84-85 1.0196699413712906 1104 86-87 1.069656297559682 1104 88-89 1.3595802580306682 1104 90-91 1.3197671929562915 1104 92-93 1.6530348956698653 1104 94-95 1.7681368584654358 1104 96-97 2.0127934526866262 1104 98-99 2.185219695719095 1104 100-101 2.731553643455328 1105 1 0.14900628645404979 1105 2 0.15223953886693664 1105 3 0.14686418145905833 1105 4 0.10854309593886313 1105 5 -0.0843568482817929 1105 6 -0.010537844241454764 1105 7 0.07517056747720119 1105 8 0.02560275462007411 1105 9 0.14268191397791696 1105 10-11 0.13374652802603748 1105 12-13 0.052094503362660305 1105 14-15 0.20446913078322382 1105 16-17 0.2250647491921356 1105 18-19 0.1875301908627307 1105 20-21 0.3306106755396456 1105 22-23 0.27057550294697563 1105 24-25 0.2801796075154428 1105 26-27 0.14605115236479094 1105 28-29 -0.026974781722643115 1105 30-31 0.11016729788774882 1105 32-33 0.3286525676110372 1105 34-35 0.2715476427166905 1105 36-37 0.35390051853234894 1105 38-39 0.35210471238539043 1105 40-41 0.21991473774142634 1105 42-43 0.3674021213017937 1105 44-45 0.5911452596939739 1105 46-47 0.8227578840406977 1105 48-49 0.5123066039083923 1105 50-51 0.6402593693706748 1105 52-53 0.634960361833798 1105 54-55 0.7721392822509401 1105 56-57 0.9508137260380778 1105 58-59 0.8401168392955327 1105 60-61 0.8198787360902102 1105 62-63 1.0052753707192856 1105 64-65 1.0424740115722813 1105 66-67 1.0746652269331527 1105 68-69 0.8513933817356403 1105 70-71 0.8786123177484733 1105 72-73 0.9925461234845336 1105 74-75 0.9723557656958697 1105 76-77 1.0542586367398812 1105 78-79 1.4085936577388587 1105 80-81 1.4123908235252856 1105 82-83 1.4539314020414302 1105 84-85 1.6282248714955116 1105 86-87 1.871149369704927 1105 88-89 1.8091827275719936 1105 90-91 1.865756801174161 1105 92-93 2.405801931623042 1105 94-95 2.425477920935574 1105 96-97 2.2565438259546617 1105 98-99 2.4554832976063388 1105 100-101 2.8026989044443376 1106 1 -0.246886680321321 1106 2 -0.07382190317932213 1106 3 -0.2625347134452234 1106 4 -0.06454072708635294 1106 5 -0.10426451777365742 1106 6 -0.22584732685573528 1106 7 -0.174031706500422 1106 8 -0.33478602220002074 1106 9 -0.34675349851939075 1106 10-11 -0.12888273867199018 1106 12-13 -0.27569036176941353 1106 14-15 -0.10657225847997154 1106 16-17 -0.16921163636348524 1106 18-19 -0.0996224734066331 1106 20-21 -0.12070850257531163 1106 22-23 -0.08770753231881656 1106 24-25 -0.18403315131694598 1106 26-27 -0.34177565218635664 1106 28-29 -0.26230076901323685 1106 30-31 -0.32175370291755456 1106 32-33 -0.29293108398880463 1106 34-35 -0.5939707396655649 1106 36-37 -0.29724683553681075 1106 38-39 -0.22048676099583986 1106 40-41 -0.07044716914668214 1106 42-43 -0.3463158959045316 1106 44-45 -0.10946349974438618 1106 46-47 -0.06760920547525018 1106 48-49 -0.3400048481044564 1106 50-51 -0.43416157163506597 1106 52-53 -0.19422620479486596 1106 54-55 -0.3154299855633482 1106 56-57 -0.45560754984515484 1106 58-59 -0.4068664726428537 1106 60-61 -0.5875140831795953 1106 62-63 -0.40038126822696185 1106 64-65 -0.27267122160611024 1106 66-67 -0.2870160175353895 1106 68-69 -0.6308632938876713 1106 70-71 -0.4266699760028736 1106 72-73 0.07768473884583926 1106 74-75 0.12627941362800144 1106 76-77 0.1250896113131148 1106 78-79 0.44853896108205404 1106 80-81 0.5249670679539626 1106 82-83 0.4227563009801898 1106 84-85 0.4486504051068465 1106 86-87 0.6549604350140115 1106 88-89 0.7422427105091884 1106 90-91 0.9705491372635855 1106 92-93 1.3200370156214802 1106 94-95 0.995004467949979 1106 96-97 1.1689936939969847 1106 98-99 1.4150512259106627 1106 100-101 1.9297023887648947 1107 1 0.046682646442100406 1107 2 0.16142151648830705 1107 3 0.041985609647554156 1107 4 0.14776313714250477 1107 5 -0.15772076523119694 1107 6 0.024668288820251405 1107 7 0.046088305719230505 1107 8 -0.06419819658654546 1107 9 0.12203414379924027 1107 10-11 0.12222367838867854 1107 12-13 0.11752021044922856 1107 14-15 0.12066032178275776 1107 16-17 0.16037006201044335 1107 18-19 0.09098042100572457 1107 20-21 0.10365752361464331 1107 22-23 0.12176183069203006 1107 24-25 0.17400633780011532 1107 26-27 0.010326144514657187 1107 28-29 0.05357220037905108 1107 30-31 0.0013651902221880619 1107 32-33 0.15015887452451437 1107 34-35 0.32262100510190095 1107 36-37 0.3148681147060728 1107 38-39 0.3491071095486191 1107 40-41 0.3092465955046393 1107 42-43 0.48572318800113834 1107 44-45 0.036589883691647174 1107 46-47 -0.035936236399521704 1107 48-49 0.03550700828609621 1107 50-51 0.08680416109319111 1107 52-53 0.19526663198566752 1107 54-55 0.09829401798858584 1107 56-57 0.5842962757619858 1107 58-59 0.5459121537221634 1107 60-61 0.5119566094311523 1107 62-63 0.3625047708157858 1107 64-65 0.3675707022616379 1107 66-67 0.6784780950279057 1107 68-69 0.6082969054889986 1107 70-71 0.8682210363766494 1107 72-73 0.7088537262447154 1107 74-75 0.9080980055500483 1107 76-77 0.8045875765504107 1107 78-79 1.0712511391091475 1107 80-81 1.0398601697047596 1107 82-83 1.4065640693258956 1107 84-85 1.1701641229372584 1107 86-87 1.4367837755071342 1107 88-89 1.70000496221828 1107 90-91 1.6604167945877037 1107 92-93 1.8984921155076684 1107 94-95 2.050965586650012 1107 96-97 2.5358148738902635 1107 98-99 2.8190647973267993 1107 100-101 3.232670720544391 1108 1 -0.014281749658010767 1108 2 0.07787195171987804 1108 3 -0.12330879656805749 1108 4 -0.039297526986317166 1108 5 -0.21576671938311165 1108 6 -0.10778779611561617 1108 7 -0.07921634857954274 1108 8 -0.11692006095776719 1108 9 -0.10171879160585462 1108 10-11 0.02840770245373392 1108 12-13 0.016911369800681086 1108 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-1.8138287818450074 2316 100-101 -1.7506679110318526 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 13.0 4 25.0 5 53.0 6 114.0 7 305.0 8 702.0 9 1201.0 10 1994.0 11 3063.0 12 4554.0 13 6459.0 14 8450.0 15 11677.0 16 15072.0 17 19015.0 18 17512.0 19 13379.0 20 13107.0 21 14043.0 22 15030.0 23 16996.0 24 19694.0 25 23276.0 26 28076.0 27 33858.0 28 40582.0 29 48880.0 30 58525.0 31 69964.0 32 85299.0 33 103855.0 34 128156.0 35 158274.0 36 179148.0 37 150842.0 38 47374.0 39 1306.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.85947007309079 21.742756247060317 16.543455051775755 20.854318628073134 2 17.017109805019125 22.26856982927512 38.398731224927694 22.31558914077806 3 20.543393567762987 25.368317665216782 30.741352184829047 23.346936582191187 4 14.475156264576919 18.812351898497994 36.50213639332027 30.210355443604815 5 13.701959502178509 37.97706462080039 34.13516840253285 14.185807474488254 6 31.496198155650223 37.663709179058365 16.593923616812305 14.246169048479112 7 27.94878033489666 33.56355364227734 20.30060990718544 18.18705611564055 8 26.328107974170745 36.164689866823494 18.966456597476185 18.540745561529576 9 25.913965172896596 17.14136233502205 20.37860219900946 36.566070293071895 10-11 25.102770704154715 27.58587361815571 26.71030752099448 20.601048156695096 12-13 26.001718137395763 24.733714826510667 27.766988624616832 21.497578411476738 14-15 22.54387719460607 26.077972887043277 26.547717848517326 24.83043206983333 16-17 21.469561362394728 29.610202735178483 27.065750860527395 21.854485041899398 18-19 21.584870540258947 27.35178479202553 30.214437753941407 20.84890691377411 20-21 22.20039182759586 26.453064651553316 30.68756413844575 20.658979382405075 22-23 21.953303800694616 26.401074429787585 30.59378366689094 21.051838102626853 24-25 21.727036442036248 26.576172643936808 30.763377722050873 20.933413191976072 26-27 21.630043273371577 26.892855490225053 30.438241778581848 21.038859457821523 28-29 21.347466782600257 26.91533924569774 30.70774845644747 21.029445515254537 30-31 21.52764017119565 26.61876651512217 30.789803940007637 21.063789373674545 32-33 21.449074391958657 26.766245533168743 30.553125811642275 21.23155426323033 34-35 21.906430147267358 26.75911800793506 30.330978389044954 21.003473455752623 36-37 21.95040582143857 26.51183879093199 30.389364679541004 21.148390708088442 38-39 21.688424899020507 26.889615158417644 30.354861196110683 21.067098746451162 40-41 21.890794372016515 26.77341037528846 30.2687338231299 21.067061429565122 42-43 21.90590747644403 26.78265731008644 30.02579988796288 21.285635325506657 44-45 21.90870647731581 27.025485940490015 29.745401813302124 21.320405768892048 46-47 22.07569954234571 27.2414760768907 29.304428170965075 21.37839620979852 48-49 22.267881505452745 27.522882714519852 28.861252832351653 21.347982947675757 50-51 22.152833545790802 27.5695288220701 28.72370279040747 21.55393484173162 52-53 22.385521037410207 27.694673010542026 28.5164660882545 21.403339863793263 54-55 22.26332884535584 27.632930211452404 28.549320981941612 21.554419961250147 56-57 22.29530941669229 27.871198528819086 28.456887055220037 21.37660499926859 58-59 22.428194847881446 27.55258695580785 28.455133161576146 21.564085034734557 60-61 22.28239777412238 27.553407927300732 28.766094772949142 21.398099525627746 62-63 22.23552776525589 27.600613788141587 28.75848212819694 21.405376318405583 64-65 22.22466855141819 27.457130361317017 28.698849744304695 21.619351342960094 66-67 22.087902164080855 27.78663846505199 28.492711265818627 21.632748105048528 68-69 22.18686654585947 27.566580788072926 28.59003370461147 21.656518961456133 70-71 22.21160764130412 27.65431278715344 28.450990987225683 21.683088584316756 72-73 22.30256553783002 27.627539882451725 28.35268215318593 21.717212426532324 74-75 22.283720496314956 27.834648754548 28.093777404608637 21.787853344528408 76-77 22.264896154569527 27.80839420961343 28.29690956476569 21.62980007105135 78-79 22.258403016398532 27.763427361934983 28.14290277607779 21.8352668455887 80-81 22.12451002928629 27.990425979717525 28.120997763972188 21.764066227023992 82-83 22.313648661255712 27.903087974624498 28.140311596230994 21.642951767888796 84-85 22.245868733130404 27.835612839570302 28.072799409795028 21.845719017504265 86-87 22.385989449769976 27.70931787717669 28.08021040753025 21.82448226552308 88-89 22.446965241559667 27.76667393102048 27.981208708865594 21.805152118554254 90-91 22.33129567015234 27.803130388051677 28.02193660105839 21.84363734073759 92-93 22.39819798168575 27.74939525955139 27.992112438061216 21.86029432070164 94-95 22.18888165770564 27.674240004298905 28.01151002033024 22.125368317665217 96-97 22.43438945096412 27.641214560153326 27.865675629685132 22.058720359197416 98-99 22.3314781796158 27.61280750161769 27.746029281923406 22.3096850368431 100-101 22.463619640076 27.351842708010267 28.19296416845718 21.99157348345655 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 564.0 1 389.5 2 295.5 3 419.0 4 348.0 5 277.5 6 544.0 7 1265.0 8 2003.5 9 2317.5 10 2047.5 11 1661.5 12 1631.0 13 1746.5 14 1968.5 15 2163.5 16 2338.5 17 2528.5 18 2694.5 19 2922.0 20 3241.5 21 3684.5 22 4387.0 23 5313.5 24 6430.5 25 7864.5 26 9696.5 27 11574.5 28 13311.5 29 15102.5 30 17342.0 31 20277.5 32 23060.0 33 25418.0 34 27958.5 35 30412.5 36 33358.0 37 36021.5 38 37747.5 39 39304.5 40 41012.0 41 43553.0 42 45766.0 43 47714.0 44 49442.5 45 50721.0 46 50841.0 47 50729.0 48 51808.5 49 51172.0 50 48852.5 51 45922.5 52 42866.5 53 40964.0 54 39618.0 55 37053.0 56 33462.0 57 29851.0 58 26595.0 59 23394.5 60 19467.0 61 15614.0 62 12446.5 63 9598.0 64 7415.5 65 5824.0 66 4469.5 67 3462.5 68 2448.0 69 1620.0 70 1140.0 71 855.0 72 688.5 73 559.5 74 470.0 75 388.5 76 265.5 77 164.0 78 106.5 79 75.5 80 50.0 81 26.0 82 13.0 83 9.0 84 5.0 85 2.5 86 2.0 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.033286662347859056 2 7.463377208040147E-5 3 0.0 4 7.463377208040147E-5 5 0.014852120643999892 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003955589920261278 14-15 0.0 16-17 0.0 18-19 0.0 20-21 7.463377208040147E-5 22-23 1.8658443020100367E-4 24-25 6.343870626834125E-4 26-27 0.0016419429857688324 28-29 0.0021270625042914422 30-31 4.104857464422081E-4 32-33 0.0 34-35 0.0 36-37 7.463377208040147E-5 38-39 0.0 40-41 0.0 42-43 0.010560678749376808 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 7.463377208040147E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 7.463377208040147E-5 74-75 7.463377208040147E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.463377208040147E-5 84-85 1.8658443020100367E-4 86-87 0.0 88-89 0.0 90-91 0.007911179840522555 92-93 0.0033585197436180663 94-95 0.0 96-97 0.0 98-99 3.7316886040200735E-4 100-101 0.013172860772190858 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1339876.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.71436655459023 #Duplication Level Percentage of deduplicated Percentage of total 1 81.3969757192402 51.047597716915114 2 11.150679088417043 13.986155513671811 3 3.229710584454338 6.076477603761276 4 1.3617834480477244 3.4161354531535517 5 0.7049900363858693 2.210650177961866 6 0.4523274360605629 1.7020457176680073 7 0.2809286351180214 1.2332782978930625 8 0.21604947625256998 1.083952483810473 9 0.16014929799009744 0.9039295599850147 >10 0.9548148539462913 11.054950655868828 >50 0.06497501049940232 2.7520885599855682 >100 0.025547021682301392 2.7908045574857288 >500 4.75285291345742E-4 0.18398504063322968 >1k 3.5646396850930656E-4 0.5419697045465693 >5k 2.37642645672871E-4 1.015978956659965 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7239 0.5402738760900263 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6395 0.4772829724541674 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4410 0.32913493487457046 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1694 0.1264296099042001 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 3.7316886040200736E-5 0.0 0.0 0.0 18-19 0.0 7.463377208040147E-5 0.0 0.0 0.0 20-21 0.0 7.463377208040147E-5 0.0 0.0 0.0 22-23 0.0 7.463377208040147E-5 0.0 3.7316886040200736E-5 0.0 24-25 0.0 7.463377208040147E-5 0.0 1.4926754416080294E-4 0.0 26-27 0.0 7.463377208040147E-5 0.0 1.4926754416080294E-4 0.0 28-29 1.1195065812060222E-4 7.463377208040147E-5 0.0 5.224364045628103E-4 0.0 30-31 1.4926754416080294E-4 7.463377208040147E-5 0.0 0.0014180416695276279 0.0 32-33 1.4926754416080294E-4 7.463377208040147E-5 0.0 0.0032092521994572633 0.0 34-35 1.4926754416080294E-4 7.463377208040147E-5 0.0 0.0055228991339497085 0.0 36-37 1.8658443020100367E-4 7.463377208040147E-5 0.0 0.009366538396090383 0.0 38-39 2.239013162412044E-4 7.463377208040147E-5 0.0 0.01582235968104511 0.0 40-41 2.239013162412044E-4 7.463377208040147E-5 0.0 0.023546955091366666 0.0 42-43 2.6121820228140513E-4 7.463377208040147E-5 0.0 0.031196916729607817 0.0 44-45 3.7316886040200735E-4 7.463377208040147E-5 0.0 0.04097394087214041 0.0 46-47 5.59753290603011E-4 7.463377208040147E-5 0.0 0.05354973146768806 0.0 48-49 5.970701766432118E-4 7.463377208040147E-5 0.0 0.06832721833960755 0.0 50-51 5.970701766432118E-4 7.463377208040147E-5 0.0 0.08355250784400944 0.0 52-53 5.970701766432118E-4 7.463377208040147E-5 0.0 0.10116607805498419 0.0 54-55 5.970701766432118E-4 7.463377208040147E-5 0.0 0.12124256274461218 0.0 56-57 5.970701766432118E-4 7.463377208040147E-5 0.0 0.14561048932886328 0.0 58-59 5.970701766432118E-4 7.463377208040147E-5 0.0 0.1769939904886721 0.0 60-61 5.970701766432118E-4 7.463377208040147E-5 0.0 0.2086013929647221 0.0 62-63 5.970701766432118E-4 7.463377208040147E-5 0.0 0.24013416166869173 0.0 64-65 5.970701766432118E-4 7.463377208040147E-5 0.0 0.27823470231573666 0.0 66-67 5.970701766432118E-4 7.463377208040147E-5 0.0 0.32469422543578663 0.0 68-69 6.717039487236132E-4 7.463377208040147E-5 0.0 0.38376685603742433 0.0 70-71 7.090208347638139E-4 7.463377208040147E-5 0.0 0.4481384844567706 0.0 72-73 7.463377208040147E-4 7.463377208040147E-5 0.0 0.5193391030214736 0.0 74-75 7.463377208040147E-4 7.463377208040147E-5 0.0 0.5935250724693927 0.0 76-77 7.463377208040147E-4 7.463377208040147E-5 0.0 0.6762192919344775 0.0 78-79 7.463377208040147E-4 7.463377208040147E-5 0.0 0.780221453328517 0.0 80-81 7.463377208040147E-4 7.463377208040147E-5 0.0 0.8885896903892598 0.0 82-83 8.209714928844162E-4 7.463377208040147E-5 0.0 1.0063617827321334 0.0 84-85 8.209714928844162E-4 7.463377208040147E-5 0.0 1.128611901399831 0.0 86-87 8.209714928844162E-4 7.463377208040147E-5 0.0 1.2667963304066943 0.0 88-89 8.209714928844162E-4 7.463377208040147E-5 0.0 1.4252065116473465 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3620 0.0 51.99444 1 GTATCAA 6380 0.0 42.613342 1 TATCAAC 8670 0.0 31.452288 2 ATCAACG 8690 0.0 31.325235 3 TCAACGC 8900 0.0 30.586098 4 CAACGCA 9155 0.0 29.682272 5 AACGCAG 9320 0.0 29.25327 6 CGTCGCA 35 0.00839496 27.141909 82-83 ACGCAGA 10515 0.0 25.839338 7 CGCAGAG 10595 0.0 25.5994 8 TGGTATC 1645 0.0 24.547796 2 GCAGAGT 11075 0.0 24.275452 9 GGACCGA 185 2.5431473E-7 23.1073 6 GAGTACT 7320 0.0 22.517128 12-13 GTACTTT 7400 0.0 22.016123 14-15 AGAGTAC 10400 0.0 22.014475 10-11 GTGGTAT 1925 0.0 21.48121 1 AGTACTT 7635 0.0 21.370384 12-13 CAGAGTA 11090 0.0 20.430616 10-11 AGGACCG 290 2.4356268E-9 19.658154 5 >>END_MODULE