FastQCFastQC Report
Thu 26 May 2016
SRR937567_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937567_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309928
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92810.7085122235725932No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72450.5530838336152827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49050.37444806126748953No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18550.14161083662613516No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16890.12893838439975328No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13380.1021430185475843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45800.041.8472751
TATCACG704.286774E-640.7472082
GTATCAA77900.038.8281971
TCAACGC95500.031.7610764
ATCAACG95450.031.7254733
TATCAAC96000.031.5437132
CAACGCA96450.031.448245
AACGCAG98900.030.6410276
GGACCGA1405.8088517E-727.141946
ACGCAGA112050.026.9612987
CGCAGAG113450.026.5867218
GTACATG72700.026.036121
TACATGG74850.024.8965342
GCAGAGT118550.024.6816799
ACATGGG73900.023.9300213
GAGTACT67700.023.50498612-13
AGGACCG2503.783498E-1022.8201835
GTACTTT71000.022.27741414-15
GTGTAGG5350.022.223571
TGCACCG4150.021.766245
Produced by FastQC (version 0.11.5)