##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937567_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1309928 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.807605456177743 33.0 31.0 34.0 30.0 34.0 2 31.944820631362944 33.0 31.0 34.0 30.0 34.0 3 31.958929803775476 34.0 31.0 34.0 30.0 34.0 4 35.56572422301073 37.0 35.0 37.0 33.0 37.0 5 35.55827266842147 37.0 35.0 37.0 33.0 37.0 6 35.63785108799873 37.0 35.0 37.0 33.0 37.0 7 35.5793639039703 37.0 35.0 37.0 33.0 37.0 8 35.56985498439609 37.0 35.0 37.0 33.0 37.0 9 37.20995123396095 39.0 37.0 39.0 34.0 39.0 10-11 37.08105025619729 39.0 37.0 39.0 33.0 39.0 12-13 36.956882363000105 39.0 37.0 39.0 33.0 39.0 14-15 38.09772636358639 40.0 38.0 41.0 33.0 41.0 16-17 38.00980168375666 40.0 38.0 41.0 33.0 41.0 18-19 37.95860421336134 40.0 38.0 41.0 33.0 41.0 20-21 37.8753500192377 40.0 38.0 41.0 33.0 41.0 22-23 37.785495844046395 40.0 38.0 41.0 33.0 41.0 24-25 37.809000189323385 40.0 38.0 41.0 33.0 41.0 26-27 37.71534885886858 40.0 37.0 41.0 33.0 41.0 28-29 37.579702090496575 40.0 37.0 41.0 32.5 41.0 30-31 37.31207020538533 39.5 37.0 41.0 32.0 41.0 32-33 37.13685599513866 39.0 36.0 41.0 32.0 41.0 34-35 37.107350938372186 40.0 36.0 41.0 31.5 41.0 36-37 36.94122539559426 39.5 36.0 41.0 31.0 41.0 38-39 36.775490332293074 39.0 36.0 41.0 31.0 41.0 40-41 36.52206647998974 39.0 35.5 41.0 30.0 41.0 42-43 36.21577789008251 39.0 35.0 41.0 30.0 41.0 44-45 35.92033875144283 39.0 35.0 40.5 29.5 41.0 46-47 35.505055621377664 39.0 35.0 40.0 27.5 41.0 48-49 35.1248557172608 39.0 35.0 40.0 26.0 41.0 50-51 34.214547288095225 37.5 33.5 39.5 23.5 40.5 52-53 34.30564656988781 38.0 34.0 39.5 24.0 40.5 54-55 34.57420102478915 38.0 34.0 40.0 24.0 41.0 56-57 34.32984790003725 38.0 34.0 40.0 23.0 41.0 58-59 34.05552442576997 38.0 34.0 40.0 22.5 41.0 60-61 33.72234199131555 37.5 33.5 40.0 22.0 41.0 62-63 33.33579059307077 37.0 33.0 40.0 20.5 41.0 64-65 32.929620940998284 36.0 32.5 39.0 18.5 41.0 66-67 32.47755754514752 36.0 32.0 39.0 17.0 40.5 68-69 31.961980734819015 35.0 31.0 39.0 12.0 40.0 70-71 31.49124608375422 35.0 31.0 38.0 8.0 40.0 72-73 31.031516617707233 35.0 30.5 37.0 7.0 39.5 74-75 30.490442604479025 35.0 30.0 37.0 2.0 39.0 76-77 30.002588310197204 34.0 29.5 36.0 2.0 39.0 78-79 29.43932376435957 34.0 29.0 36.0 2.0 38.0 80-81 28.875805387777035 34.0 28.5 35.0 2.0 37.0 82-83 28.36766715422527 34.0 27.5 35.0 2.0 37.0 84-85 27.850523845585407 33.5 26.5 35.0 2.0 36.0 86-87 27.305667181707697 33.0 26.0 35.0 2.0 36.0 88-89 26.810264762643442 33.0 25.0 35.0 2.0 35.0 90-91 26.236143131530895 32.0 24.5 35.0 2.0 35.0 92-93 25.587683826897358 32.0 21.5 34.0 2.0 35.0 94-95 24.8212836888745 31.5 18.5 34.0 2.0 35.0 96-97 23.96309262799177 31.0 6.0 34.0 2.0 35.0 98-99 22.8864418502391 31.0 2.0 34.0 2.0 35.0 100-101 20.06095640371074 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0631425588601644 1101 2 0.28054883853651447 1101 3 0.2341812215842971 1101 4 0.09534923431569098 1101 5 0.0680804389317089 1101 6 0.25152492038711216 1101 7 0.13590783119242644 1101 8 0.19307236809838457 1101 9 0.13635098899078457 1101 10-11 0.1473702835680939 1101 12-13 0.11039358367151664 1101 14-15 0.418101775175181 1101 16-17 0.3919927873563793 1101 18-19 0.33913202350006344 1101 20-21 0.26304547369017683 1101 22-23 0.34190950014021126 1101 24-25 0.17232236417746094 1101 26-27 0.15161049791812786 1101 28-29 0.20873304890036337 1101 30-31 0.19913198033408008 1101 32-33 0.2452358074695411 1101 34-35 0.2255461550143636 1101 36-37 0.18860216962148968 1101 38-39 0.08294690498541257 1101 40-41 0.13311379996453354 1101 42-43 0.15521754095111362 1101 44-45 0.35439780294746726 1101 46-47 0.26601633499416977 1101 48-49 0.45555300590243775 1101 50-51 0.37663160422473396 1101 52-53 0.28272173177746396 1101 54-55 0.5023858153772451 1101 56-57 0.4323603638939417 1101 58-59 0.2823468493140453 1101 60-61 0.5817433601146789 1101 62-63 0.7226177776211955 1101 64-65 0.6416535115408522 1101 66-67 0.6482779759944322 1101 68-69 0.750354466095537 1101 70-71 0.7951032910963214 1101 72-73 0.7494705689137007 1101 74-75 0.8949241678437154 1101 76-77 0.8031301749477535 1101 78-79 0.906836674106696 1101 80-81 1.0010340233856674 1101 82-83 0.9128822390955875 1101 84-85 0.9173213544395722 1101 86-87 0.9089288795814383 1101 88-89 0.7731811173002185 1101 90-91 0.8579011603370112 1101 92-93 0.688999994791665 1101 94-95 1.091724343825227 1101 96-97 1.1098289838906084 1101 98-99 1.5129362519661207 1101 100-101 1.6536420865780386 1102 1 0.1946172343828927 1102 2 0.1467419337066822 1102 3 0.2779233785408657 1102 4 0.07188383225545891 1102 5 0.15746676075341526 1102 6 0.10913102899431948 1102 7 0.08808751526144931 1102 8 0.02973045120714346 1102 9 0.07727693992765694 1102 10-11 0.1731875924214421 1102 12-13 0.21007324314367537 1102 14-15 0.24674933261778875 1102 16-17 0.28600582279641884 1102 18-19 0.2760605378767025 1102 20-21 0.3357718967078256 1102 22-23 0.36656270681016423 1102 24-25 0.41558908623923685 1102 26-27 0.26308815189668877 1102 28-29 0.27194563104268354 1102 30-31 0.3362473861062014 1102 32-33 0.3356891856913933 1102 34-35 0.30402712464931625 1102 36-37 0.4415294192724417 1102 38-39 0.3520946641222551 1102 40-41 0.181779576970321 1102 42-43 0.20303204307941058 1102 44-45 0.4334284005437681 1102 46-47 0.2499565995132471 1102 48-49 0.31626189628006074 1102 50-51 0.2229204750858642 1102 52-53 0.12254364158480513 1102 54-55 0.07416503577313449 1102 56-57 0.09198591922390875 1102 58-59 0.10987614417133074 1102 60-61 0.23421234794951573 1102 62-63 0.371233332316109 1102 64-65 0.3891991923773972 1102 66-67 0.42630430625956706 1102 68-69 0.35087399644761774 1102 70-71 0.2711689834889235 1102 72-73 0.31077711820714526 1102 74-75 0.37239702409342 1102 76-77 0.38218693465613285 1102 78-79 0.5577757374046968 1102 80-81 0.5677867641464154 1102 82-83 0.8355642536050532 1102 84-85 0.7033810183660023 1102 86-87 0.5552406031592696 1102 88-89 0.5489541518100545 1102 90-91 0.6032042785924396 1102 92-93 0.6685378690328498 1102 94-95 0.8773081703628236 1102 96-97 0.8708651466377297 1102 98-99 1.1139083644963002 1102 100-101 1.1215098699718524 1103 1 0.23878513574079818 1103 2 0.26983448069110594 1103 3 0.2925635013528769 1103 4 0.2099857944110184 1103 5 0.22474085685684742 1103 6 0.2335709578832379 1103 7 0.23006124911940162 1103 8 0.24648900982516864 1103 9 0.33367198641478524 1103 10-11 0.3314918090444152 1103 12-13 0.44276221780432934 1103 14-15 0.3619484784285021 1103 16-17 0.42584386044462263 1103 18-19 0.36779713073544684 1103 20-21 0.30260716823137557 1103 22-23 0.311110426251453 1103 24-25 0.36250933068579627 1103 26-27 0.40943764340609334 1103 28-29 0.3727207435921116 1103 30-31 0.31648912987974853 1103 32-33 0.3988433081626539 1103 34-35 0.3755480828827089 1103 36-37 0.3002436485352007 1103 38-39 0.31781313846313 1103 40-41 0.4933791480916554 1103 42-43 0.5828953959864265 1103 44-45 0.5109742208946315 1103 46-47 0.7091442187639032 1103 48-49 0.7947178349739659 1103 50-51 0.7945640847670319 1103 52-53 0.6076350236034855 1103 54-55 0.7603405673891075 1103 56-57 0.7601305730034014 1103 58-59 0.7984032385451556 1103 60-61 0.8028652693285991 1103 62-63 0.7746491082359199 1103 64-65 0.7792468524999734 1103 66-67 0.8429008757654728 1103 68-69 0.8706529919188739 1103 70-71 0.7440170749877915 1103 72-73 0.7487783723739483 1103 74-75 0.7150425756815117 1103 76-77 0.7496593693007512 1103 78-79 0.6962739752378972 1103 80-81 0.6865385648926718 1103 82-83 0.6210254631891665 1103 84-85 0.5737522642281867 1103 86-87 0.746780463095778 1103 88-89 0.7399556833921359 1103 90-91 0.6619732773017013 1103 92-93 0.6844658320465271 1103 94-95 0.7772582623737065 1103 96-97 1.1626654290482605 1103 98-99 1.2689264143293606 1103 100-101 1.4219811637350013 1104 1 0.26331232657408776 1104 2 0.2209486723612848 1104 3 0.14604663069869517 1104 4 0.237672242492728 1104 5 0.20223792404824792 1104 6 0.21410739774297838 1104 7 0.2497641190919424 1104 8 0.23771768437941887 1104 9 0.2547080096641281 1104 10-11 0.27990055942450454 1104 12-13 0.44784422161307447 1104 14-15 0.5097505485274496 1104 16-17 0.48966218891366964 1104 18-19 0.3410215298656638 1104 20-21 0.41893953605747924 1104 22-23 0.3956299738685942 1104 24-25 0.42960404501600635 1104 26-27 0.35110667561141184 1104 28-29 0.3287476767424451 1104 30-31 0.4292823762898692 1104 32-33 0.46204981187815974 1104 34-35 0.3761396972089628 1104 36-37 0.5298265236889605 1104 38-39 0.6137289326364552 1104 40-41 0.6311667218022947 1104 42-43 0.4327790022983322 1104 44-45 0.5334677374814234 1104 46-47 0.5197598834986437 1104 48-49 0.6741908848284979 1104 50-51 0.4941291931178924 1104 52-53 0.4966182278197451 1104 54-55 0.6324338256498976 1104 56-57 0.7417723854377698 1104 58-59 0.6006205520832921 1104 60-61 0.8894795818020214 1104 62-63 0.7446981736414671 1104 64-65 0.6352273476003276 1104 66-67 0.8951122180409996 1104 68-69 0.8563793776422273 1104 70-71 0.7046425693394376 1104 72-73 0.9410902339655962 1104 74-75 0.875128681288345 1104 76-77 0.8932574408585232 1104 78-79 0.7722833253164865 1104 80-81 0.828482137379666 1104 82-83 0.7772516068467219 1104 84-85 0.8366687942448046 1104 86-87 0.9915248333434583 1104 88-89 1.0973676738711866 1104 90-91 1.2554030970522483 1104 92-93 1.153477505405597 1104 94-95 1.3516158693488762 1104 96-97 1.432963358306253 1104 98-99 1.3498586111017552 1104 100-101 1.5970220668838806 1105 1 0.2103217912722144 1105 2 0.24021026985206717 1105 3 0.2580853510651515 1105 4 0.22162425973915845 1105 5 0.13063617679124917 1105 6 0.14248415786051538 1105 7 0.23289100641311222 1105 8 -0.027840523490716862 1105 9 0.15158685993949206 1105 10-11 0.22346045773635126 1105 12-13 0.332940440826647 1105 14-15 0.3513237339541462 1105 16-17 0.3174625728841036 1105 18-19 0.41136058537690445 1105 20-21 0.45903379523704757 1105 22-23 0.502773515171107 1105 24-25 0.37431426602093154 1105 26-27 0.3697903083697227 1105 28-29 0.41342161258883436 1105 30-31 0.573696466294443 1105 32-33 0.5137888689779899 1105 34-35 0.586048591838555 1105 36-37 0.7316805896965519 1105 38-39 0.6384737193898999 1105 40-41 0.6959293754687295 1105 42-43 0.6014038340367662 1105 44-45 0.621548721694154 1105 46-47 0.5637102000242677 1105 48-49 0.5020949940783126 1105 50-51 0.3749474454030022 1105 52-53 0.49845491283456766 1105 54-55 0.5710541579539239 1105 56-57 0.5387210400227787 1105 58-59 0.6031730349721229 1105 60-61 0.7157451119760267 1105 62-63 0.5165013909006007 1105 64-65 0.4687143999287926 1105 66-67 0.522898491635587 1105 68-69 0.47758680530670006 1105 70-71 0.5912343949464081 1105 72-73 0.5418801346743365 1105 74-75 0.6097667104332807 1105 76-77 0.7624603307756317 1105 78-79 0.764965553578449 1105 80-81 0.649315172853246 1105 82-83 0.7288062945985878 1105 84-85 0.5162025350970083 1105 86-87 0.49009931159828923 1105 88-89 0.5034376585319933 1105 90-91 0.5144238089347262 1105 92-93 0.5239503291487821 1105 94-95 0.8633861187894851 1105 96-97 1.0792134324791753 1105 98-99 1.0329082651405663 1105 100-101 1.1905126514786204 1106 1 -0.013975038475194879 1106 2 -0.0075934911604775834 1106 3 -0.10684601149284845 1106 4 0.010671013646089023 1106 5 0.10792406339079719 1106 6 0.007339559366521087 1106 7 0.2027506255661038 1106 8 0.0976553333764727 1106 9 -0.01363156305119162 1106 10-11 -0.0679741258114035 1106 12-13 -0.1628754315124894 1106 14-15 -0.17829890600831533 1106 16-17 -0.20348700296386113 1106 18-19 -0.11965395043354476 1106 20-21 -0.18670797287580854 1106 22-23 0.01753817336325625 1106 24-25 -0.2846726980979639 1106 26-27 0.03901055249648522 1106 28-29 -0.037775220100563445 1106 30-31 -0.047159496599910256 1106 32-33 -0.033145174161134605 1106 34-35 0.13286326761026857 1106 36-37 -0.0010942168921417306 1106 38-39 -0.09149559234509042 1106 40-41 0.012021881079000707 1106 42-43 0.08433984685802898 1106 44-45 0.2588761755616815 1106 46-47 0.16855284971939 1106 48-49 0.26705895960212445 1106 50-51 0.1902094435114492 1106 52-53 0.24576890592139478 1106 54-55 0.17151952188451958 1106 56-57 0.1838695884914756 1106 58-59 0.12671035065805825 1106 60-61 0.0031773920953099832 1106 62-63 0.21711525265919818 1106 64-65 0.299754593694594 1106 66-67 0.33614161378432783 1106 68-69 -0.019294692076144315 1106 70-71 -0.04034606275871866 1106 72-73 0.27050843240886735 1106 74-75 0.0452666137336557 1106 76-77 0.1434250216672055 1106 78-79 0.23391243437650644 1106 80-81 0.2011464129572076 1106 82-83 0.18103619759512313 1106 84-85 0.23571140710673433 1106 86-87 0.374381209573194 1106 88-89 0.4233279273665964 1106 90-91 0.34210338930175865 1106 92-93 0.4750844638270024 1106 94-95 0.765901080705266 1106 96-97 0.8911295271068695 1106 98-99 0.9922437819723307 1106 100-101 0.9704882089001075 1107 1 0.11757298615120249 1107 2 0.1594828880123238 1107 3 0.16785228119658413 1107 4 0.2521741064945857 1107 5 0.12192493147372119 1107 6 0.12532124233197095 1107 7 0.08095907700801774 1107 8 0.04021006745939815 1107 9 0.13902009079225763 1107 10-11 0.10086218236288857 1107 12-13 0.2148080479966339 1107 14-15 0.1142683158596185 1107 16-17 0.3433033938082346 1107 18-19 0.31090644801167144 1107 20-21 0.3421642710215451 1107 22-23 0.0926205563721112 1107 24-25 0.10851721565146022 1107 26-27 0.0487580567580963 1107 28-29 0.0711308741810015 1107 30-31 0.3121662115060033 1107 32-33 0.2186917426411199 1107 34-35 0.059736011019019486 1107 36-37 0.03121859363542967 1107 38-39 0.16489560752493304 1107 40-41 0.09285543466614143 1107 42-43 0.0345206166390426 1107 44-45 0.24147766003196836 1107 46-47 0.21595623912246964 1107 48-49 0.04278779765616747 1107 50-51 0.028634721698075793 1107 52-53 0.03394430716726049 1107 54-55 0.19287775642695237 1107 56-57 0.295442249214112 1107 58-59 0.19424841415419536 1107 60-61 0.044420302549404767 1107 62-63 0.14732938786190175 1107 64-65 0.15168365337650158 1107 66-67 0.25555708845352854 1107 68-69 0.536213391036739 1107 70-71 0.22367723484095947 1107 72-73 0.3189634630463729 1107 74-75 0.3055789780956992 1107 76-77 0.1771553776539463 1107 78-79 0.4499402033306623 1107 80-81 0.5377821794283513 1107 82-83 0.5220630572445408 1107 84-85 0.629961206453487 1107 86-87 0.7443969466071145 1107 88-89 0.9325424578137564 1107 90-91 1.1323580673715163 1107 92-93 1.0385403552729926 1107 94-95 1.4697771436824993 1107 96-97 1.5273591374637299 1107 98-99 1.487546919922348 1107 100-101 1.1206750407126833 1108 1 -0.21668884424568802 1108 2 -0.11686257449567705 1108 3 -0.09447043730421711 1108 4 -0.12850022492496294 1108 5 -0.16759193884308843 1108 6 -0.09421665873207985 1108 7 -0.009035677336491688 1108 8 -0.023581434578709093 1108 9 -0.056113480921631265 1108 10-11 0.15871622801452645 1108 12-13 0.0657707942175918 1108 14-15 0.14129451044937014 1108 16-17 0.04513119193230608 1108 18-19 0.0875303152628959 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-0.595782016672942 2316 70-71 -0.47751049261492895 2316 72-73 -0.3810649989201238 2316 74-75 -0.6602797737991004 2316 76-77 -0.9067354691055485 2316 78-79 -0.48064617713867364 2316 80-81 -0.5814242114024175 2316 82-83 -0.7010707651655785 2316 84-85 -0.6340452790373057 2316 86-87 -1.031713229984998 2316 88-89 -0.9920640315146052 2316 90-91 -1.1347251886774536 2316 92-93 -1.3200145443426123 2316 94-95 -1.2113947857829928 2316 96-97 -1.039470519265766 2316 98-99 -1.3638096605482666 2316 100-101 -1.17103932906263 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5251.0 3 1109.0 4 966.0 5 1077.0 6 1169.0 7 1483.0 8 1838.0 9 2312.0 10 2739.0 11 3745.0 12 5000.0 13 6462.0 14 7380.0 15 9744.0 16 12496.0 17 16287.0 18 14005.0 19 9537.0 20 9395.0 21 9687.0 22 10519.0 23 11426.0 24 13036.0 25 14544.0 26 17250.0 27 20376.0 28 24110.0 29 29296.0 30 35317.0 31 44146.0 32 56525.0 33 74492.0 34 99915.0 35 139384.0 36 191083.0 37 237223.0 38 155423.0 39 14180.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.28328226602532 18.763923531524522 14.892636122264403 22.060158080185758 2 15.304819515062995 19.98416919668625 40.896522581860104 23.814488706390655 3 19.207153470291985 25.11476800290279 29.688187756395585 25.989890770409644 4 12.533683024368136 16.572583855420213 36.95073704581503 33.94299607439662 5 13.60888532286392 37.48562573785327 33.49673928189173 15.408749657391088 6 31.551085999603796 35.834353796390275 17.45087436028248 15.163685843723446 7 28.538823263057093 33.37280061679103 20.06919012522449 18.019185994927383 8 26.886220949443775 34.42995741087491 19.29590263244851 19.38791900723281 9 26.97816717465878 16.777201161051984 20.26907647416506 35.975555190124176 10-11 25.171612461045846 26.838972665925194 26.487486479829514 21.50192839319945 12-13 26.243749653422466 24.401697226041215 27.554147361899606 21.800405758636714 14-15 23.06183184912726 25.278465512190685 26.582318176151283 25.077384462530777 16-17 21.78657794765512 29.005923057241432 26.89022858097898 22.317270414124465 18-19 21.62016012298914 26.673843582747104 30.458666126167127 21.24733016809663 20-21 22.648978461811435 25.681853988182368 30.469018873638852 21.20014867636734 22-23 22.234943301969114 25.712807476017964 30.53009427620243 21.522154945810495 24-25 21.96565877282178 25.928196311191893 30.626682845179964 21.479462070806363 26-27 21.602697254105383 26.306219561440003 30.46150363427229 21.629579550182328 28-29 21.438039424450746 26.25756073831355 30.798554701960825 21.50584513527488 30-31 21.517137035087934 25.976521864176526 30.709247145357306 21.797093955378234 32-33 21.35201155634733 26.144247049433062 30.64252541528233 21.861215978937278 34-35 21.9908752221626 26.2830165082063 30.20539355528622 21.520714714344887 36-37 22.086933246661378 25.91008421837509 30.226041736370675 21.776940798592857 38-39 21.634111902437795 26.17128520298122 30.38086531553594 21.813737579045046 40-41 21.750249016837554 26.184794391433435 30.359023968707042 21.705932623021965 42-43 21.91125441965013 26.07870820404845 29.981231544160554 22.028805832140865 44-45 21.73545251965369 26.41799714941625 29.811086682756493 22.035463648173565 46-47 21.993511681153777 26.721425640370605 29.262753967431436 22.02230871104418 48-49 22.157486915479694 26.77318864577987 28.94461096736346 22.124713471376968 50-51 22.055515485089323 27.034026582143905 28.732908118973338 22.177549813793433 52-53 22.291225725356025 27.079843577325214 28.578046277272694 22.050884420046064 54-55 21.92511140062506 26.963941492349658 28.684442075307704 22.426505031717582 56-57 22.084483562869444 27.319283327343218 28.59946012495125 21.99677298483609 58-59 22.224465310261134 26.820399764169878 28.699814409954417 22.255320515614567 60-61 22.174385787570547 26.818665971358293 29.05824456554569 21.948703675525476 62-63 22.00666811958263 26.965355601182218 29.026087488481817 22.001888790753338 64-65 21.91928360162843 26.90779834893758 28.921311490452407 22.25160655898159 66-67 21.883607156566327 27.30138448574605 28.55954909778265 22.25545925990497 68-69 21.87173674230765 27.090081302553866 28.771499025157027 22.26668292998146 70-71 21.903814419272596 27.191559062247173 28.635290986922296 22.269335531557928 72-73 22.068802830444405 27.218826095051885 28.53854297414589 22.17382810035782 74-75 21.855534981068114 27.491933445717702 28.3102695580725 22.342262015141685 76-77 21.996265824648233 27.323029109820162 28.2930505566659 22.387654508865705 78-79 22.17832995952191 27.20451874052191 28.312446802246217 22.304704497709963 80-81 21.897334665408003 27.76194250438449 28.172232207684356 22.168490622523148 82-83 22.066022078218513 27.53640864394409 28.190045646772212 22.207523631065193 84-85 22.080572423886068 27.403310185786967 28.043181057039 22.472936333287965 86-87 22.08919984081151 27.186978045062883 28.235427696732927 22.488394417392684 88-89 22.1139674748943 27.222084376258053 28.148969984795315 22.51497816405233 90-91 21.950388601085795 27.390612627403687 28.257666971144452 22.401331800366062 92-93 22.22990257380561 27.097809659732814 28.197816120913544 22.474471645548036 94-95 22.057886246998194 27.066433340508482 28.120874369004273 22.754806043489047 96-97 22.17527230379197 27.029580360252687 28.039424167661675 22.755723168293667 98-99 22.12065981282898 27.084052611596622 27.86727734108108 22.928010234493318 100-101 22.343160306586583 26.42957977179934 28.467896596026577 22.759363325587504 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1948.0 1 1214.5 2 746.5 3 917.0 4 613.0 5 490.5 6 957.0 7 2119.0 8 3531.0 9 3980.5 10 3126.5 11 2258.5 12 2087.0 13 2206.5 14 2387.0 15 2526.0 16 2593.0 17 2671.5 18 2798.5 19 2925.5 20 3113.0 21 3528.5 22 4102.5 23 4758.0 24 5681.5 25 6883.5 26 8425.0 27 10035.5 28 11313.5 29 12873.0 30 14868.5 31 17306.5 32 19822.5 33 21948.0 34 24484.0 35 26513.5 36 28847.5 37 31692.0 38 33500.0 39 34391.5 40 35740.5 41 39177.0 42 42398.5 43 44997.5 44 47166.5 45 48646.5 46 48679.0 47 48725.0 48 51148.0 49 51214.0 50 48611.0 51 46301.5 52 44360.5 53 43291.5 54 42205.0 55 39124.0 56 34948.5 57 31245.0 58 28093.0 59 24772.0 60 21124.5 61 17861.0 62 14744.5 63 11578.5 64 9022.0 65 7141.0 66 5436.5 67 4282.0 68 3165.0 69 2105.0 70 1619.0 71 1284.5 72 1081.5 73 891.0 74 675.0 75 585.5 76 446.0 77 266.5 78 156.5 79 117.5 80 92.0 81 61.0 82 36.5 83 29.0 84 23.5 85 13.0 86 9.0 87 7.5 88 5.0 89 4.0 90 3.5 91 2.0 92 0.5 93 0.5 94 1.0 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31566620455475414 2 0.27589302618159167 3 0.27459524492949233 4 0.27726714750734394 5 0.28810743796605615 6 0.1919952852370512 7 0.1920716253107041 8 0.19481986796220863 9 0.19031580361668735 10-11 0.20016367311791183 12-13 0.1908883541690841 14-15 0.19046848376399314 16-17 0.19691921998766346 18-19 0.1905066538008196 20-21 0.1824527760304383 22-23 0.1822237558094796 24-25 0.1856972291606867 26-27 0.18138401499929768 28-29 0.1920716253107041 30-31 0.1972627503191015 32-33 0.19806432109245697 34-35 0.19913308212359762 36-37 0.17676544054329704 38-39 0.1685970526624364 40-41 0.17447523833370995 42-43 0.1843612778717609 44-45 0.18905619240141444 46-47 0.190964694242737 48-49 0.18833096170171185 50-51 0.1915372447951338 52-53 0.2159278983272363 54-55 0.1811168247415125 56-57 0.16932228336213898 58-59 0.16848254255195705 60-61 0.16145925577589 62-63 0.16894058299387446 64-65 0.16519991938488224 66-67 0.1641311583537416 68-69 0.17416987803909834 70-71 0.16840620247830415 72-73 0.16394030816960933 74-75 0.16897875303070092 76-77 0.17378817767083382 78-79 0.17611654991724737 80-81 0.16745195155764286 82-83 0.1655434497163203 84-85 0.1592453936399558 86-87 0.15607728058336032 88-89 0.16088670522349321 90-91 0.16981849384088288 92-93 0.16165010596002224 94-95 0.1659251500845848 96-97 0.16424566846422092 98-99 0.16745195155764286 100-101 0.1460003908611771 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1309928.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.599287263926634 #Duplication Level Percentage of deduplicated Percentage of total 1 79.38077015884973 44.92895013452471 2 11.73091655422747 13.27923031843746 3 3.641242424940769 6.1827517802045815 4 1.6020673295068404 3.6270347599563784 5 0.886235510776349 2.508014912896166 6 0.5804605518349459 1.971219211121009 7 0.37936024960842785 1.5030063820871622 8 0.2667998453151915 1.2080544869572571 9 0.2040671652526559 1.039505049654323 >10 1.1835463993188837 12.361075251006506 >50 0.09250206636561503 3.639908842733814 >100 0.04933581183669978 4.930281194477332 >500 0.0013479660832119787 0.4991911157186683 >1k 0.001078372866569583 1.0609432454812473 >5k 2.6959321664239575E-4 1.2608333147434987 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9281 0.7085122235725932 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7245 0.5530838336152827 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4905 0.37444806126748953 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1855 0.14161083662613516 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1689 0.12893838439975328 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1338 0.1021430185475843 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 3.817003682645153E-5 0.0 0.0 0.0 0.0 14-15 7.634007365290306E-5 0.0 0.0 0.0 3.817003682645153E-5 16-17 7.634007365290306E-5 0.0 0.0 0.0 7.634007365290306E-5 18-19 1.145101104793546E-4 0.0 0.0 0.0 7.634007365290306E-5 20-21 1.5268014730580612E-4 0.0 0.0 0.0 7.634007365290306E-5 22-23 1.5268014730580612E-4 0.0 0.0 1.145101104793546E-4 7.634007365290306E-5 24-25 1.5268014730580612E-4 0.0 0.0 1.9085018413225765E-4 7.634007365290306E-5 26-27 1.5268014730580612E-4 0.0 0.0 2.671902577851607E-4 7.634007365290306E-5 28-29 3.0536029461161225E-4 0.0 0.0 9.924209574877399E-4 7.634007365290306E-5 30-31 3.0536029461161225E-4 0.0 0.0 0.00274824265150451 7.634007365290306E-5 32-33 3.0536029461161225E-4 0.0 0.0 0.006183545965885148 7.634007365290306E-5 34-35 3.0536029461161225E-4 0.0 0.0 0.009313488985654173 7.634007365290306E-5 36-37 3.0536029461161225E-4 0.0 0.0 0.013817553331175455 7.634007365290306E-5 38-39 3.0536029461161225E-4 0.0 0.0 0.020688159959936732 7.634007365290306E-5 40-41 3.0536029461161225E-4 0.0 0.0 0.02988713883511155 7.634007365290306E-5 42-43 3.435303314380638E-4 0.0 0.0 0.03870441734202185 7.634007365290306E-5 44-45 5.343805155703215E-4 0.0 0.0 0.048590456880072797 7.634007365290306E-5 46-47 5.343805155703215E-4 0.0 0.0 0.06172094954837212 7.634007365290306E-5 48-49 5.343805155703215E-4 0.0 0.0 0.07706530435260564 7.634007365290306E-5 50-51 5.343805155703215E-4 0.0 0.0 0.09641751302361656 7.634007365290306E-5 52-53 5.343805155703215E-4 0.0 0.0 0.11916685497218168 7.634007365290306E-5 54-55 5.343805155703215E-4 0.0 0.0 0.14726000207645001 7.634007365290306E-5 56-57 6.107205892232245E-4 0.0 0.0 0.17928466297384282 7.634007365290306E-5 58-59 6.107205892232245E-4 0.0 0.0 0.21676763913741826 7.634007365290306E-5 60-61 6.107205892232245E-4 0.0 0.0 0.2647473754282678 7.634007365290306E-5 62-63 6.107205892232245E-4 0.0 0.0 0.3120400510562413 7.634007365290306E-5 64-65 6.107205892232245E-4 0.0 0.0 0.3612412285255373 7.634007365290306E-5 66-67 6.107205892232245E-4 0.0 0.0 0.4250997001361907 7.634007365290306E-5 68-69 6.107205892232245E-4 0.0 0.0 0.49869153113758924 7.634007365290306E-5 70-71 6.870606628761275E-4 0.0 0.0 0.5861390855069897 7.634007365290306E-5 72-73 8.015707733554821E-4 0.0 0.0 0.6779761941114321 1.5268014730580612E-4 74-75 8.397408101819336E-4 0.0 0.0 0.7793939819593139 1.5268014730580612E-4 76-77 8.397408101819336E-4 0.0 0.0 0.8864991052943367 1.5268014730580612E-4 78-79 8.397408101819336E-4 0.0 0.0 1.0042154988671133 1.5268014730580612E-4 80-81 0.001145101104793546 0.0 0.0 1.1341844742611809 1.5268014730580612E-4 82-83 0.001145101104793546 0.0 0.0 1.2913686859125082 1.5268014730580612E-4 84-85 0.001145101104793546 0.0 0.0 1.4546601034560678 1.5268014730580612E-4 86-87 0.001145101104793546 0.0 0.0 1.627035989764323 1.5268014730580612E-4 88-89 0.001145101104793546 0.0 0.0 1.8261690718879207 1.5268014730580612E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4580 0.0 41.847275 1 TATCACG 70 4.286774E-6 40.747208 2 GTATCAA 7790 0.0 38.828197 1 TCAACGC 9550 0.0 31.761076 4 ATCAACG 9545 0.0 31.725473 3 TATCAAC 9600 0.0 31.543713 2 CAACGCA 9645 0.0 31.44824 5 AACGCAG 9890 0.0 30.641027 6 GGACCGA 140 5.8088517E-7 27.14194 6 ACGCAGA 11205 0.0 26.961298 7 CGCAGAG 11345 0.0 26.586721 8 GTACATG 7270 0.0 26.03612 1 TACATGG 7485 0.0 24.896534 2 GCAGAGT 11855 0.0 24.681679 9 ACATGGG 7390 0.0 23.930021 3 GAGTACT 6770 0.0 23.504986 12-13 AGGACCG 250 3.783498E-10 22.820183 5 GTACTTT 7100 0.0 22.277414 14-15 GTGTAGG 535 0.0 22.22357 1 TGCACCG 415 0.0 21.76624 5 >>END_MODULE