FastQCFastQC Report
Thu 26 May 2016
SRR937567_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937567_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309928
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94320.7200395746941817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79430.606369205025009No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55560.42414544921552944No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21710.16573429990045255No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15270.11657129246798298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46950.045.8477861
GTATCAA78000.038.440631
TATCAAC102000.029.1129262
ATCAACG101850.029.0625043
TCAACGC102800.028.7477114
CAACGCA104400.028.3526445
AACGCAG107350.027.5640356
TATACCG700.00838140327.1498835
GTAAGGT4350.024.0292074
ACGCAGA122650.024.0139627
CGCAGAG124000.023.790838
TAAGGTG4300.023.2036785
GTACATG74950.022.760331
GTACCTG8800.022.678921
ACTACCG1050.002193082822.6249035
GCAGAGT131900.021.969739
TACATGG78250.021.8586922
GAGTACT75650.021.72735412-13
TACCTGG8800.021.5964972
AGTACTT76850.021.48080812-13
Produced by FastQC (version 0.11.5)