##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937567_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1309928 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.792527528230558 31.0 31.0 34.0 30.0 34.0 2 32.09382195051942 33.0 31.0 34.0 30.0 34.0 3 32.17649977708699 33.0 31.0 34.0 30.0 34.0 4 35.91712368924094 37.0 35.0 37.0 35.0 37.0 5 35.814289793026795 37.0 35.0 37.0 35.0 37.0 6 35.86880423962233 37.0 35.0 37.0 35.0 37.0 7 35.795418526819795 37.0 35.0 37.0 33.0 37.0 8 35.766063478298044 37.0 35.0 37.0 33.0 37.0 9 37.390413824271256 39.0 37.0 39.0 34.0 39.0 10-11 37.2866623203718 39.0 37.0 39.0 34.0 39.0 12-13 37.15435619362286 39.0 37.0 39.0 33.0 39.0 14-15 38.228123988494026 40.0 38.0 41.0 33.0 41.0 16-17 38.17490655974984 40.0 37.5 41.0 33.0 41.0 18-19 38.16076188920307 40.0 37.5 41.0 33.0 41.0 20-21 38.056623722830565 40.0 37.0 41.0 33.0 41.0 22-23 37.99003762038829 40.0 37.0 41.0 33.0 41.0 24-25 38.005156390274884 40.0 37.0 41.0 33.0 41.0 26-27 37.83184533806438 40.0 37.0 41.0 33.0 41.0 28-29 37.64072758197397 39.5 37.0 41.0 32.0 41.0 30-31 37.643727746868535 39.0 37.0 41.0 33.0 41.0 32-33 37.523610076279 39.0 36.0 41.0 33.0 41.0 34-35 37.33202588233857 39.0 36.0 41.0 32.0 41.0 36-37 37.13652773282196 39.0 36.0 41.0 31.5 41.0 38-39 36.966548924826405 39.0 35.0 41.0 31.0 41.0 40-41 36.732769281975806 39.0 35.0 40.5 31.0 41.0 42-43 36.44911247030371 39.0 35.0 40.0 30.0 41.0 44-45 36.06418291692368 38.5 35.0 40.0 30.0 41.0 46-47 35.60520959930622 38.0 35.0 40.0 28.5 41.0 48-49 35.18358299082087 38.0 34.5 40.0 26.5 41.0 50-51 34.859147983705974 38.0 34.0 40.0 26.0 41.0 52-53 34.56612577179814 38.0 34.0 40.0 24.5 41.0 54-55 34.28121889141999 38.0 33.5 40.0 24.0 41.0 56-57 33.9539753329954 37.5 33.0 40.0 23.0 41.0 58-59 33.64315634141724 37.0 33.0 40.0 23.0 41.0 60-61 33.25582360251861 36.5 32.5 39.5 22.0 41.0 62-63 32.81430582444226 36.0 32.0 39.0 20.5 40.0 64-65 32.33744946287124 35.5 31.0 39.0 19.0 40.0 66-67 31.854977525482315 35.0 31.0 38.0 18.0 40.0 68-69 31.35558824607154 35.0 30.0 38.0 14.0 40.0 70-71 30.920955197537573 34.5 30.0 37.0 12.0 39.0 72-73 30.362293194740474 34.0 29.0 36.5 8.0 39.0 74-75 29.84283181976414 34.0 29.0 36.0 7.0 39.0 76-77 28.74616658320152 32.5 27.0 35.0 7.0 37.0 78-79 29.3513983211291 34.0 29.0 35.0 4.0 37.0 80-81 29.137674742428594 34.0 29.0 35.0 2.0 37.0 82-83 28.710076431681742 33.5 28.5 35.0 2.0 36.5 84-85 28.232080694511453 33.0 27.0 35.0 2.0 36.0 86-87 27.74096515228318 33.0 26.5 35.0 2.0 35.5 88-89 27.173191961695604 32.5 25.5 35.0 2.0 35.0 90-91 26.455758637115935 32.0 25.0 34.0 2.0 35.0 92-93 25.828763489291013 32.0 23.5 34.0 2.0 35.0 94-95 25.15315078385988 31.0 20.5 34.0 2.0 35.0 96-97 24.31762089214064 31.0 14.5 34.0 2.0 35.0 98-99 23.103031617004902 31.0 2.0 34.0 2.0 35.0 100-101 20.19579587580386 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24673484011834645 1101 2 0.17640095959964697 1101 3 0.18578100853582669 1101 4 0.09062884985223008 1101 5 0.04531365265147258 1101 6 -0.040701446345451586 1101 7 0.08310052799546241 1101 8 0.005132993492665605 1101 9 0.04129867285821831 1101 10-11 0.17281574068805838 1101 12-13 0.19410664783897147 1101 14-15 0.2123414114632851 1101 16-17 0.20824625984064227 1101 18-19 0.1792922418044114 1101 20-21 0.16529184546860876 1101 22-23 0.16824353026446204 1101 24-25 -0.00513514530759096 1101 26-27 -0.02046020939562254 1101 28-29 -0.15467002481967995 1101 30-31 -0.07278304145426517 1101 32-33 0.049917309613739747 1101 34-35 0.09245438998291888 1101 36-37 0.08041220633583634 1101 38-39 0.18899414802775283 1101 40-41 0.20248943480935822 1101 42-43 0.1608891284455254 1101 44-45 0.25156775991775504 1101 46-47 -0.0584157223453019 1101 48-49 -0.0279679553085046 1101 50-51 0.018677557105384324 1101 52-53 0.08262580686894694 1101 54-55 -0.10816259625950408 1101 56-57 -0.14179521276187756 1101 58-59 -0.13012641769200428 1101 60-61 0.02733124590238134 1101 62-63 -0.15900777311241399 1101 64-65 -0.07498195227277193 1101 66-67 -0.11116334179460097 1101 68-69 -0.10701214193394293 1101 70-71 -0.060986221797847406 1101 72-73 -0.48350692279770513 1101 74-75 -0.2474055597195708 1101 76-77 -0.1488055057350124 1101 78-79 -0.3385717774140673 1101 80-81 -0.4263178887378878 1101 82-83 -0.16041960952565404 1101 84-85 -0.06925117314358786 1101 86-87 0.013324614793184253 1101 88-89 0.029684339346502497 1101 90-91 -0.15397021672618294 1101 92-93 -0.31902393953846797 1101 94-95 -0.8405118473239632 1101 96-97 -0.8713590973411769 1101 98-99 -1.1463163874503586 1101 100-101 -0.9748860495045193 1102 1 0.2718088766594384 1102 2 0.20449235973327262 1102 3 0.10035835849723185 1102 4 -0.012384131027509682 1102 5 0.062395946581233375 1102 6 0.07482641563308334 1102 7 0.15228388549334682 1102 8 0.08735805205554215 1102 9 0.06768618159010487 1102 10-11 0.12907157126132773 1102 12-13 0.26603412020329387 1102 14-15 0.23319306200186674 1102 16-17 0.1486722237467646 1102 18-19 0.25966157927241085 1102 20-21 0.25258475110880596 1102 22-23 0.23371879968949827 1102 24-25 0.11929096528524497 1102 26-27 0.14511308968696568 1102 28-29 0.17960149228765232 1102 30-31 0.21256063031096062 1102 32-33 0.20287801099089364 1102 34-35 0.137480950116867 1102 36-37 0.24235232595707146 1102 38-39 0.37212781450688937 1102 40-41 0.1643638584602698 1102 42-43 0.27313196830031217 1102 44-45 0.20064756911769877 1102 46-47 0.156416361616742 1102 48-49 0.22236253701326092 1102 50-51 0.11756206725673479 1102 52-53 0.3262462764632019 1102 54-55 0.24915528189929148 1102 56-57 0.25613386600083743 1102 58-59 0.10520267484494639 1102 60-61 -0.02321507266359646 1102 62-63 0.04219765179158941 1102 64-65 0.018970217125335864 1102 66-67 -0.16701319005435522 1102 68-69 -0.17811427792505086 1102 70-71 -0.2971227620843706 1102 72-73 -0.17771185844785364 1102 74-75 -0.24178473056397465 1102 76-77 -0.03532119539959311 1102 78-79 0.06056321818980592 1102 80-81 -0.03199470928547754 1102 82-83 0.07719587272033124 1102 84-85 0.024679529906155295 1102 86-87 0.023436606521485714 1102 88-89 -0.005538136993553877 1102 90-91 0.08601416650537885 1102 92-93 -0.013848939605551891 1102 94-95 -0.32929187896894163 1102 96-97 -0.3061282312366309 1102 98-99 0.10864055016038421 1102 100-101 -0.05547340457312444 1103 1 0.2920057790636754 1103 2 0.15341985087338372 1103 3 0.2220754252454853 1103 4 0.16239329004516634 1103 5 0.06198695975355406 1103 6 0.03694941853301259 1103 7 0.06385397566668871 1103 8 0.1593945923748663 1103 9 0.2282596729995916 1103 10-11 0.19903061905792185 1103 12-13 0.31874012334494495 1103 14-15 0.22576350630338737 1103 16-17 0.1595790594910511 1103 18-19 0.22241590262444078 1103 20-21 0.18099020200674687 1103 22-23 0.23317734929821654 1103 24-25 0.14852973241966083 1103 26-27 0.12186283524095387 1103 28-29 0.1306127200241889 1103 30-31 0.140578279631292 1103 32-33 0.14420980947144102 1103 34-35 0.2606140835803714 1103 36-37 0.2849572018650264 1103 38-39 0.24657911653006437 1103 40-41 0.2298780177894102 1103 42-43 0.30922895838917697 1103 44-45 0.3306975752279655 1103 46-47 0.2627143573582842 1103 48-49 0.0803888031553015 1103 50-51 0.11424245007196987 1103 52-53 0.18525174716378245 1103 54-55 0.06713250530243897 1103 56-57 0.05207352327255421 1103 58-59 0.10717535613048312 1103 60-61 0.11846295970389775 1103 62-63 -0.014730237185212047 1103 64-65 -0.05792398953386879 1103 66-67 -9.640920108004991E-4 1103 68-69 -0.09769640667249391 1103 70-71 -0.14602219949332707 1103 72-73 -0.08483706015373471 1103 74-75 0.017724993688812418 1103 76-77 -0.1195528576701328 1103 78-79 0.12464770764311695 1103 80-81 0.3049854604284441 1103 82-83 0.44248265856555236 1103 84-85 0.3239022319309264 1103 86-87 0.3243177791228433 1103 88-89 0.34186406074199027 1103 90-91 0.3267662473429418 1103 92-93 0.18113028992927127 1103 94-95 -0.07228981931662659 1103 96-97 -0.049635509596544836 1103 98-99 0.04850343030182103 1103 100-101 0.10800402711447887 1104 1 0.3064920621548062 1104 2 0.24241692679201066 1104 3 0.17922242946303868 1104 4 0.09469065524850606 1104 5 0.12259757188146381 1104 6 0.08815667003432281 1104 7 0.18619733796523974 1104 8 0.17536404523266924 1104 9 0.12712210153965486 1104 10-11 0.16089354275345613 1104 12-13 0.22928854247614083 1104 14-15 0.22721957572436935 1104 16-17 0.21799339529832906 1104 18-19 0.211414310593959 1104 20-21 0.08905250891678662 1104 22-23 0.11232496534051961 1104 24-25 -0.022127115653965745 1104 26-27 0.05033859237408933 1104 28-29 0.06628247061832582 1104 30-31 0.2099990304119146 1104 32-33 0.2933211937295397 1104 34-35 0.3493213235984882 1104 36-37 0.36464209374382506 1104 38-39 0.33332364846115325 1104 40-41 0.09853825937751282 1104 42-43 0.15176722269931275 1104 44-45 0.07319812871808296 1104 46-47 0.01725802887637684 1104 48-49 0.24296699224589702 1104 50-51 0.2975735326986282 1104 52-53 0.33726240908283245 1104 54-55 0.15904952863721178 1104 56-57 0.19713248719418175 1104 58-59 0.3942741217326997 1104 60-61 0.3842231887064216 1104 62-63 0.28842226794318293 1104 64-65 0.26425571423637706 1104 66-67 0.3376868803404136 1104 68-69 0.276682926512013 1104 70-71 0.31939109915156294 1104 72-73 0.17183352917313854 1104 74-75 0.37821172508396117 1104 76-77 0.41098142334850607 1104 78-79 0.4404271365515626 1104 80-81 0.2631990240136659 1104 82-83 0.19496234019620573 1104 84-85 0.3619129930648093 1104 86-87 0.259565637047217 1104 88-89 0.2660384418206192 1104 90-91 0.10485047338003639 1104 92-93 0.22981720928139993 1104 94-95 0.3141160960917091 1104 96-97 0.5352078641852032 1104 98-99 0.564063162679016 1104 100-101 0.34970466107883524 1105 1 0.2718347363700566 1105 2 0.09248624050912468 1105 3 0.02688456912866144 1105 4 0.04151930155418171 1105 5 0.06157206527458925 1105 6 0.09348170160745894 1105 7 0.03430054368377711 1105 8 0.12678276876695094 1105 9 0.13956165937094056 1105 10-11 0.09037296800327255 1105 12-13 0.10792739581653876 1105 14-15 0.09618604676142439 1105 16-17 -0.052587779311032534 1105 18-19 0.009734054514005663 1105 20-21 -0.18179101589247892 1105 22-23 -0.11925059829190587 1105 24-25 0.22603304552202985 1105 26-27 0.11100526558198709 1105 28-29 0.06227666574053359 1105 30-31 0.06541229855718456 1105 32-33 0.06417219423461518 1105 34-35 0.16552036213056454 1105 36-37 0.15032487474005052 1105 38-39 0.2967329208470133 1105 40-41 0.32394730842694486 1105 42-43 0.3032374350884979 1105 44-45 0.048534172925606356 1105 46-47 0.012001446643019165 1105 48-49 0.11747645811210106 1105 50-51 0.3907279445973231 1105 52-53 0.48364027093104056 1105 54-55 0.42155017692021346 1105 56-57 0.2598225041967197 1105 58-59 0.3239560916159263 1105 60-61 0.2487617116677825 1105 62-63 0.07867526885101483 1105 64-65 0.23550582345162496 1105 66-67 0.09928670727805766 1105 68-69 0.4307301514169204 1105 70-71 0.2884368125642247 1105 72-73 0.23510711768000547 1105 74-75 0.07414947870278255 1105 76-77 0.0493287525860957 1105 78-79 0.1598958108163373 1105 80-81 0.07734808658477377 1105 82-83 0.20505172615656875 1105 84-85 0.1786505153108422 1105 86-87 -0.2234451433733753 1105 88-89 0.07004321879023934 1105 90-91 0.4094161611510607 1105 92-93 0.5493884874711981 1105 94-95 0.3481365860394696 1105 96-97 0.15777773741141843 1105 98-99 0.4140212754209216 1105 100-101 0.1597075158601733 1106 1 0.04354790165021072 1106 2 0.11077476807526665 1106 3 0.07081465409410725 1106 4 0.00948158243065933 1106 5 -0.06860154551834796 1106 6 -0.08416587865925607 1106 7 -0.018864958327142745 1106 8 -2.868081806255418E-4 1106 9 -0.022021725849015183 1106 10-11 -0.10460940662120066 1106 12-13 -0.17787811205905513 1106 14-15 -0.0402653229730916 1106 16-17 -0.05678251740456375 1106 18-19 -0.1232004390862187 1106 20-21 0.023998566688270273 1106 22-23 0.014215381526092585 1106 24-25 0.006868289135155692 1106 26-27 0.04329020778647674 1106 28-29 0.09744084530570518 1106 30-31 0.05600856146634925 1106 32-33 0.2104599173370474 1106 34-35 -0.24976377319875098 1106 36-37 0.0909373185590141 1106 38-39 0.1561109276386503 1106 40-41 0.008975891485654586 1106 42-43 -0.05776445019246523 1106 44-45 -0.01206779550256698 1106 46-47 -0.11695148210166195 1106 48-49 -0.09377219207205911 1106 50-51 0.12326570447454799 1106 52-53 0.36450850334389173 1106 54-55 0.08935851006464901 1106 56-57 0.08651844922695773 1106 58-59 0.11848868035410476 1106 60-61 0.28625632807894164 1106 62-63 0.027067330125298383 1106 64-65 -0.13401286320831218 1106 66-67 -0.1953611493421974 1106 68-69 0.3962066972966731 1106 70-71 0.39094251446604744 1106 72-73 0.39447123445070886 1106 74-75 0.1762876738006689 1106 76-77 -0.030649312755844704 1106 78-79 -0.010699448924640365 1106 80-81 -0.06265609006735318 1106 82-83 -0.11455572641841627 1106 84-85 -0.1642572062386094 1106 86-87 -0.05288967275486911 1106 88-89 -0.2688224102082657 1106 90-91 -0.4896988805358866 1106 92-93 -0.4169065086005297 1106 94-95 -0.36185231641255555 1106 96-97 -0.6712628720904164 1106 98-99 -0.9407896635493138 1106 100-101 -0.9810251634525216 1107 1 0.1385336316279293 1107 2 0.08989858628221725 1107 3 0.08670639106013311 1107 4 0.010703687992453581 1107 5 0.08499336043529837 1107 6 -0.02725358655663257 1107 7 -0.008258342953347153 1107 8 0.12192180814854225 1107 9 0.0642186749240139 1107 10-11 -0.0017728608152864922 1107 12-13 0.039341937261632154 1107 14-15 0.043518257650717374 1107 16-17 0.12511019433613768 1107 18-19 0.06383093556430453 1107 20-21 -0.04835226490121869 1107 22-23 -0.06267227448029189 1107 24-25 0.08389876660501017 1107 26-27 0.05138229247063464 1107 28-29 -0.012851514352199445 1107 30-31 0.013792573706801647 1107 32-33 -0.0033811555940417293 1107 34-35 -0.04624124790189654 1107 36-37 -0.019623710337477007 1107 38-39 -0.17756414262029097 1107 40-41 -0.08469559005060745 1107 42-43 0.1507434898564668 1107 44-45 0.20318330596856526 1107 46-47 -0.19643950716451997 1107 48-49 -0.30199691400667206 1107 50-51 -0.11481767513229357 1107 52-53 -0.16836953100600027 1107 54-55 0.0029103028498624894 1107 56-57 -0.17987510271131413 1107 58-59 0.016460324595300335 1107 60-61 -0.16758008921191703 1107 62-63 -0.2712091130625538 1107 64-65 -0.013587211600544435 1107 66-67 -0.19868241519138152 1107 68-69 -0.11579951992289139 1107 70-71 -0.19311216020896893 1107 72-73 0.013848154599205742 1107 74-75 -0.15087022757250068 1107 76-77 -0.006301526731007812 1107 78-79 -0.006048173351963726 1107 80-81 -0.15220442022014424 1107 82-83 -0.1719415844262393 1107 84-85 0.12870016798029837 1107 86-87 -0.11077382975663497 1107 88-89 -0.16904355311944386 1107 90-91 0.033257063043574675 1107 92-93 -0.0803752515534164 1107 94-95 -0.2932856474019019 1107 96-97 -0.3538359862530207 1107 98-99 -0.2693037626223109 1107 100-101 -0.3153418391314098 1108 1 0.09291423521518638 1108 2 -0.08598102351801629 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1.0 3 6.0 4 16.0 5 59.0 6 92.0 7 238.0 8 521.0 9 920.0 10 1545.0 11 2433.0 12 3678.0 13 5326.0 14 6958.0 15 9663.0 16 13162.0 17 17424.0 18 15587.0 19 10246.0 20 9213.0 21 9366.0 22 10276.0 23 11247.0 24 13015.0 25 15685.0 26 18926.0 27 23100.0 28 28079.0 29 34854.0 30 42834.0 31 52042.0 32 65353.0 33 84177.0 34 111590.0 35 152030.0 36 202248.0 37 228187.0 38 105669.0 39 4162.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.65975015976734 20.16588316407285 15.868084479318814 21.306282196840996 2 16.156689527974056 21.242846935098722 39.27055532823178 23.32990820869544 3 19.709556555780164 25.305131274390654 30.295176528786318 24.690135641042865 4 13.398140966526404 17.68150615911714 36.384900544152046 32.53545233020441 5 13.567668041781916 37.88781075644575 33.46623738226116 15.07828381951116 6 31.46875248105239 36.690718879205576 16.809549837853684 15.030978801888347 7 28.291325935471264 33.836897905839095 19.872924313397377 17.99885184529226 8 27.04904391691757 34.96367739295595 18.92256673649239 19.06471195363409 9 26.811702627930696 16.85146053828913 20.14416059508614 36.192676238694034 10-11 25.40574749146518 27.266994827196612 26.08074642270415 21.24651125863406 12-13 26.297557514097726 24.64988058016245 27.515345935812935 21.53721596992689 14-15 22.94794065017314 25.647172974392486 26.492906480356172 24.911979895078204 16-17 21.594163954049385 29.32042829834922 26.94816050958526 22.137247238016137 18-19 21.557482548659163 26.81143543767291 30.57587897960804 21.05520303405989 20-21 22.430087760548673 25.7350785692038 30.83635131091175 20.998482359335778 22-23 22.09412664628877 25.755086585704735 30.861978936053912 21.28880783195258 24-25 21.95484474301964 26.03507834418001 30.91848769967746 21.091589213122887 26-27 21.60951120957442 26.36118441996264 30.806805368625405 21.222499001837537 28-29 21.410036880424517 26.361411365216973 31.109909161628785 21.118642592729724 30-31 21.4565228012532 26.030705504424674 31.09060192621274 21.42216976810939 32-33 21.442018187259144 26.226517793344367 30.889178641879557 21.44228537751693 34-35 22.02628694096164 26.19075246883798 30.527212182654313 21.25574840754606 36-37 22.133353894259837 25.985970221264072 30.497935764408425 21.382740120067666 38-39 21.672068999212172 26.219799866862914 30.71584850465064 21.39228262927428 40-41 21.70923806622862 26.1887203540377 30.67224869972678 21.4297928800069 42-43 21.923653654693045 26.113517813841842 30.2722314180832 21.690597113381912 44-45 21.87719477711752 26.514625231310422 29.944279380240747 21.66390061133131 46-47 22.142018492619442 26.904341307308492 29.33504742245375 21.61859277761831 48-49 22.33447181829841 26.918158860639668 29.011060149870833 21.73630917119109 50-51 22.24271104976762 27.069961097098467 28.78238345924356 21.904944393890354 52-53 22.50409946195516 27.292034371354763 28.45816716643968 21.745699000250397 54-55 22.07969079157555 27.182461903367788 28.60739026761446 22.130457037442206 56-57 22.221679359476244 27.514298495795188 28.50259708930567 21.761425055422894 58-59 22.37256551505121 27.01713376613066 28.59951081280803 22.0107899060101 60-61 22.255803372399093 27.00667517604021 29.047932405445188 21.68958904611551 62-63 22.156523106613495 27.135079179924393 28.948155929180842 21.760241784281273 64-65 22.105604277487007 27.029768048320214 28.825362920710145 22.039264753482634 66-67 22.047776671694933 27.47128086429178 28.485916783212513 21.995025680800776 68-69 22.044226858270072 27.276728186587356 28.646688978325525 22.032355976817048 70-71 22.121902883211902 27.406468141760463 28.461411619569933 22.010217355457705 72-73 22.33943392308585 27.3731838696478 28.358772390543603 21.92860981672275 74-75 22.152209892452106 27.729692013606854 28.038487611532847 22.079610482408192 76-77 22.160683640627575 27.565637195326765 28.267126132123295 22.006553031922365 78-79 22.314699739222306 27.48116690382983 28.140783310227736 22.063350046720124 80-81 22.04651706047966 27.910427137980104 28.10394922469021 21.939106576850026 82-83 22.250006011833577 27.752455977155794 28.0320539515305 21.96548405948013 84-85 22.173992204906153 27.633802354559588 27.954316520812426 22.237888919721833 86-87 22.20591513426692 27.462196395527084 28.056427528841283 22.275460941364717 88-89 22.238397835606232 27.466242419430685 28.01264649660134 22.28271324836174 90-91 22.161608518867894 27.68231225541639 28.068377856410166 22.087701369305552 92-93 22.267675034573102 27.40132780570812 28.05961956797647 22.271377591742304 94-95 22.057090160680588 27.40994161511167 27.964132379794922 22.568835844412824 96-97 22.216068364062757 27.36119847808429 27.86550100463537 22.557232153217583 98-99 22.171535841857477 27.495234394502816 27.54248965766272 22.79074010597699 100-101 22.19853109044277 27.21109523784072 28.075238283579413 22.515135388137093 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 959.0 1 678.0 2 632.5 3 830.0 4 606.5 5 492.0 6 881.0 7 1897.5 8 3055.0 9 3489.5 10 2984.5 11 2364.5 12 2241.5 13 2370.5 14 2554.5 15 2705.0 16 2929.5 17 3087.5 18 3130.0 19 3307.0 20 3582.5 21 3838.5 22 4366.5 23 5093.5 24 5999.5 25 7315.5 26 8795.0 27 10298.0 28 11535.0 29 12932.0 30 14989.0 31 17412.0 32 20008.0 33 22330.0 34 24988.0 35 27055.0 36 29027.5 37 31554.0 38 33618.5 39 34821.0 40 35996.0 41 39394.5 42 43010.5 43 45628.0 44 47367.0 45 48788.5 46 48788.0 47 48582.5 48 51491.0 49 51742.5 50 48781.5 51 46306.0 52 43931.5 53 42663.0 54 41852.0 55 38874.5 56 34286.0 57 30494.5 58 27657.0 59 24459.5 60 20708.0 61 17181.0 62 14148.0 63 11286.0 64 8768.5 65 6812.0 66 5120.5 67 4099.0 68 2959.5 69 1881.5 70 1445.0 71 1136.5 72 952.5 73 825.5 74 670.5 75 544.0 76 372.5 77 221.5 78 140.5 79 100.5 80 66.5 81 42.5 82 26.5 83 16.5 84 14.5 85 9.5 86 3.5 87 2.0 88 2.5 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016107755540762547 2 0.0 3 0.0 4 0.0 5 0.03526911402764121 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004084193940430313 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.160808838348367E-4 24-25 2.2902022095870917E-4 26-27 0.0016031415467109642 28-29 0.0014504613994051581 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.397408101819338E-4 42-43 0.012214411784464491 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.9085018413225768E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.779108470083852E-4 84-85 1.1451011047935459E-4 86-87 0.0 88-89 0.0 90-91 0.01347402299973739 92-93 0.001717651657190319 94-95 0.0 96-97 0.0 98-99 0.0016031415467109642 100-101 0.01900867833957286 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1309928.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.62446215471382 #Duplication Level Percentage of deduplicated Percentage of total 1 78.58363751775096 43.711725710858865 2 12.206405462911677 13.579494774336451 3 3.8125175475409687 6.362077141121249 4 1.6579245448590498 3.6888464440353332 5 0.9138024813602528 2.5414885770653473 6 0.5562860410751722 1.8565867103388896 7 0.3947850961534992 1.537179604816476 8 0.27662511123833344 1.2309698424896143 9 0.22229476263319722 1.112852395015323 >10 1.2238762285801197 12.582374549595201 >50 0.09671672778768856 3.6788417290710496 >100 0.05197437528456583 5.060771898373037 >500 0.002194158486592888 0.8554864081959389 >1k 5.48539621648222E-4 0.45211484096097465 >5k 4.114047162361664E-4 1.7491893737263553 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9432 0.7200395746941817 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7943 0.606369205025009 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5556 0.42414544921552944 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2171 0.16573429990045255 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1527 0.11657129246798298 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 3.817003682645153E-5 0.0 0.0 0.0 0.0 16-17 7.634007365290306E-5 0.0 0.0 0.0 0.0 18-19 7.634007365290306E-5 0.0 0.0 0.0 0.0 20-21 7.634007365290306E-5 0.0 0.0 0.0 0.0 22-23 1.5268014730580612E-4 0.0 0.0 1.145101104793546E-4 0.0 24-25 1.5268014730580612E-4 0.0 0.0 1.9085018413225765E-4 0.0 26-27 1.5268014730580612E-4 0.0 0.0 2.671902577851607E-4 0.0 28-29 3.0536029461161225E-4 0.0 0.0 9.924209574877399E-4 0.0 30-31 3.0536029461161225E-4 0.0 0.0 0.002671902577851607 3.817003682645153E-5 32-33 3.0536029461161225E-4 0.0 0.0 0.0060690358554057935 7.634007365290306E-5 34-35 3.0536029461161225E-4 0.0 0.0 0.009160808838348368 7.634007365290306E-5 36-37 3.817003682645153E-4 0.0 0.0 0.013664873183869648 7.634007365290306E-5 38-39 4.5804044191741835E-4 0.0 0.0 0.020497309775804474 7.634007365290306E-5 40-41 4.962104787438699E-4 0.0 0.0 0.029696288650979293 7.634007365290306E-5 42-43 5.72550552396773E-4 0.0 0.0 0.038246376900104434 7.634007365290306E-5 44-45 6.870606628761275E-4 0.0 0.0 0.04767437599623796 7.634007365290306E-5 46-47 7.252306997025791E-4 0.0 0.0 0.060804868664537284 7.634007365290306E-5 48-49 8.397408101819336E-4 0.0 0.0 0.07626373357925015 7.634007365290306E-5 50-51 8.397408101819336E-4 0.0 0.0 0.09557777221343464 7.634007365290306E-5 52-53 8.397408101819336E-4 0.0 0.0 0.11805992390421458 7.634007365290306E-5 54-55 8.397408101819336E-4 0.0 0.0 0.14611490097165647 7.634007365290306E-5 56-57 8.397408101819336E-4 0.0 0.0 0.17791054164809056 7.634007365290306E-5 58-59 8.397408101819336E-4 0.0 0.0 0.21428658674369888 7.634007365290306E-5 60-61 8.397408101819336E-4 0.0 0.0 0.2616937724821517 7.634007365290306E-5 62-63 8.397408101819336E-4 0.0 0.0 0.30879559792599287 7.634007365290306E-5 64-65 8.397408101819336E-4 0.0 0.0 0.3579967753952889 7.634007365290306E-5 66-67 8.397408101819336E-4 0.0 0.0 0.42074831593797524 7.634007365290306E-5 68-69 8.397408101819336E-4 0.0 0.0 0.4923553050243983 7.634007365290306E-5 70-71 9.160808838348367E-4 0.0 0.0 0.5783523979943936 7.634007365290306E-5 72-73 9.160808838348367E-4 0.0 0.0 0.6687390451994308 7.634007365290306E-5 74-75 9.160808838348367E-4 0.0 0.0 0.7704621933419242 7.634007365290306E-5 76-77 9.160808838348367E-4 0.0 0.0 0.8774909766032942 7.634007365290306E-5 78-79 9.160808838348367E-4 0.0 0.0 0.9942531192554095 7.634007365290306E-5 80-81 9.160808838348367E-4 0.0 0.0 1.123382353839295 7.634007365290306E-5 82-83 9.160808838348367E-4 0.0 0.0 1.280108525048705 7.634007365290306E-5 84-85 9.542509206612883E-4 0.0 0.0 1.4417586310087271 7.634007365290306E-5 86-87 0.0010305909943141914 0.0 0.0 1.61276039599123 7.634007365290306E-5 88-89 0.001068761031140643 0.0 0.0 1.8098322961261992 7.634007365290306E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4695 0.0 45.847786 1 GTATCAA 7800 0.0 38.44063 1 TATCAAC 10200 0.0 29.112926 2 ATCAACG 10185 0.0 29.062504 3 TCAACGC 10280 0.0 28.747711 4 CAACGCA 10440 0.0 28.352644 5 AACGCAG 10735 0.0 27.564035 6 TATACCG 70 0.008381403 27.149883 5 GTAAGGT 435 0.0 24.029207 4 ACGCAGA 12265 0.0 24.013962 7 CGCAGAG 12400 0.0 23.79083 8 TAAGGTG 430 0.0 23.203678 5 GTACATG 7495 0.0 22.76033 1 GTACCTG 880 0.0 22.67892 1 ACTACCG 105 0.0021930828 22.624903 5 GCAGAGT 13190 0.0 21.96973 9 TACATGG 7825 0.0 21.858692 2 GAGTACT 7565 0.0 21.727354 12-13 TACCTGG 880 0.0 21.596497 2 AGTACTT 7685 0.0 21.480808 12-13 >>END_MODULE