FastQCFastQC Report
Thu 26 May 2016
SRR937566_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937566_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276304
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89790.7035157768055259No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70090.5491638355752234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48870.3829025059860347No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17330.1357826975391443No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16860.1321001892965939No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13220.1035803382266294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43300.047.8841551
GTATCAA72850.038.709571
ATCAACG93750.029.9148123
TATCAAC94100.029.7577082
TCAACGC95700.029.3064144
CAACGCA96300.029.0239565
AACGCAG98150.028.5074066
GTACATG72500.027.7456931
TACATGG74950.026.1336082
ACATGGG73900.025.0856723
ACGCAGA111550.024.9591037
CGCAGAG111850.024.8496828
GTGTAGC6150.024.7438951
TGGTATC21850.023.9339872
GCAGAGT116850.023.2975239
GAGTACT66050.023.23116312-13
GTGGTAT22850.023.1009581
CATGGGA42850.021.743424
GTACTTT71200.021.24682214-15
AGAGTAC106750.021.02730810-11
Produced by FastQC (version 0.11.5)