##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937566_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1276304 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77039796161416 33.0 31.0 34.0 30.0 34.0 2 31.89281002018328 33.0 31.0 34.0 30.0 34.0 3 31.930348882397922 33.0 31.0 34.0 30.0 34.0 4 35.53436093595256 37.0 35.0 37.0 33.0 37.0 5 35.52410554225326 37.0 35.0 37.0 33.0 37.0 6 35.61272314432925 37.0 35.0 37.0 33.0 37.0 7 35.55164130175883 37.0 35.0 37.0 33.0 37.0 8 35.54430449171984 37.0 35.0 37.0 33.0 37.0 9 37.16100474495104 39.0 37.0 39.0 34.0 39.0 10-11 37.049669984580476 39.0 37.0 39.0 33.0 39.0 12-13 36.92110421968434 39.0 37.0 39.0 33.0 39.0 14-15 38.053083356316364 40.0 38.0 41.0 33.0 41.0 16-17 37.9946501773872 40.0 38.0 41.0 33.0 41.0 18-19 37.965401659792654 40.0 38.0 41.0 33.0 41.0 20-21 37.79778367849666 40.0 38.0 41.0 33.0 41.0 22-23 37.65691206797127 40.0 37.0 41.0 32.5 41.0 24-25 37.498261386002085 40.0 37.0 41.0 32.0 41.0 26-27 37.5854310571776 40.0 37.0 41.0 32.0 41.0 28-29 37.520137443743806 40.0 37.0 41.0 32.0 41.0 30-31 37.277991371961534 39.5 37.0 41.0 32.0 41.0 32-33 37.018645244393184 39.0 36.0 41.0 31.0 41.0 34-35 36.73448684639396 39.0 35.5 41.0 30.5 41.0 36-37 36.72767381438905 39.0 36.0 41.0 31.0 41.0 38-39 36.66625506149005 39.0 36.0 41.0 30.5 41.0 40-41 36.458955311587204 39.0 35.0 41.0 30.0 41.0 42-43 36.1808432787173 39.0 35.0 41.0 30.0 41.0 44-45 35.892685049956754 39.0 35.0 41.0 29.0 41.0 46-47 35.47141276686432 39.0 35.0 40.0 27.0 41.0 48-49 35.07974510774862 39.0 35.0 40.0 25.5 41.0 50-51 34.16546959031704 37.5 33.5 39.5 23.5 40.5 52-53 34.267109951861 38.0 34.0 39.5 24.0 40.5 54-55 34.54948233336258 38.0 34.0 40.0 24.0 41.0 56-57 34.2723700623049 38.0 34.0 40.0 23.0 41.0 58-59 34.028892411212375 38.0 34.0 40.0 22.5 41.0 60-61 33.71081537000589 37.5 33.0 40.0 21.5 41.0 62-63 33.34169602226429 37.0 33.0 40.0 20.0 41.0 64-65 32.96802564279356 36.5 32.5 39.5 18.5 41.0 66-67 32.54734882911908 36.0 32.0 39.0 17.0 40.5 68-69 32.06329213102834 35.0 31.5 39.0 12.5 40.0 70-71 31.614152270932316 35.0 31.0 38.0 8.0 40.0 72-73 31.141021261392268 35.0 31.0 37.5 7.0 39.5 74-75 30.6525130376462 35.0 30.0 37.0 4.0 39.0 76-77 30.188247470822 34.5 30.0 36.0 2.0 39.0 78-79 29.649599546816432 34.0 29.0 36.0 2.0 38.5 80-81 29.122745834848125 34.0 29.0 35.0 2.0 37.0 82-83 28.61114005754115 34.0 28.5 35.0 2.0 37.0 84-85 28.127684705211298 34.0 27.0 35.0 2.0 36.0 86-87 27.59350593588988 33.0 26.0 35.0 2.0 36.0 88-89 27.09452371848713 33.0 25.5 35.0 2.0 35.5 90-91 26.558045340295102 32.5 25.0 35.0 2.0 35.0 92-93 25.994602774887486 32.0 23.5 35.0 2.0 35.0 94-95 25.330763673858264 32.0 20.5 34.0 2.0 35.0 96-97 24.522117379558473 31.5 12.5 34.0 2.0 35.0 98-99 23.551665590642983 31.0 2.0 34.0 2.0 35.0 100-101 21.038841059810203 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23332239957532863 1101 2 0.2830267152278303 1101 3 0.26634833970724614 1101 4 0.1624926372860216 1101 5 0.10687918759231962 1101 6 0.031798540171870116 1101 7 0.04220938608393254 1101 8 0.20862209423153644 1101 9 0.1676158177544238 1101 10-11 0.20707639658598254 1101 12-13 0.3376718475415359 1101 14-15 0.4421250890773365 1101 16-17 0.5054930144522132 1101 18-19 0.456100754695413 1101 20-21 0.5343017277724371 1101 22-23 0.47148302645963724 1101 24-25 0.41794239086119234 1101 26-27 0.5067843620478172 1101 28-29 0.38576866034904356 1101 30-31 0.4174817321003772 1101 32-33 0.48225188593808355 1101 34-35 0.4899646243133162 1101 36-37 0.47191134950389113 1101 38-39 0.3652792431619645 1101 40-41 0.6483968301972638 1101 42-43 0.6910074918868006 1101 44-45 0.7981136860290761 1101 46-47 0.879986362320544 1101 48-49 0.7773619219454488 1101 50-51 0.7957400772724839 1101 52-53 0.6997993514817153 1101 54-55 0.7286252606554626 1101 56-57 0.9327721373428446 1101 58-59 0.9421960308569837 1101 60-61 1.0229340768867061 1101 62-63 0.9201363764807482 1101 64-65 0.9157922720335634 1101 66-67 1.2007782484629388 1101 68-69 1.1723570781407702 1101 70-71 1.1215636278141012 1101 72-73 1.1503723406400148 1101 74-75 1.0322470332541336 1101 76-77 0.9851984569484209 1101 78-79 1.0957408285792987 1101 80-81 1.1160318820784667 1101 82-83 1.12150651328119 1101 84-85 1.3576433789134654 1101 86-87 1.3264846387723708 1101 88-89 1.3264435934668342 1101 90-91 1.248724965327451 1101 92-93 1.7040668785734567 1101 94-95 1.7441170562433612 1101 96-97 1.8879809160038796 1101 98-99 2.2493732550092957 1101 100-101 2.568756615559117 1102 1 0.18164675939148012 1102 2 0.11406939415100936 1102 3 0.1809257089346481 1102 4 0.21133789883121779 1102 5 0.07486868201122832 1102 6 0.1793327927435513 1102 7 0.1410288214089519 1102 8 0.19167659193344377 1102 9 -0.005136644491145148 1102 10-11 0.10389320551573178 1102 12-13 0.3443024012732039 1102 14-15 0.40561272313293273 1102 16-17 0.45279329897837783 1102 18-19 0.21438418651856495 1102 20-21 0.26464994401231934 1102 22-23 0.369518920528364 1102 24-25 0.3284182061380605 1102 26-27 0.28682244477926844 1102 28-29 0.2688457012770371 1102 30-31 0.25244780782810494 1102 32-33 0.16882903629921486 1102 34-35 0.435383753225544 1102 36-37 0.5503930741701168 1102 38-39 0.5410074112512646 1102 40-41 0.6531670206109226 1102 42-43 0.5282852331868639 1102 44-45 0.671778820631836 1102 46-47 0.6247305076477474 1102 48-49 0.7763868727005416 1102 50-51 0.7252047303694411 1102 52-53 0.6796566506719088 1102 54-55 0.8186081890861914 1102 56-57 0.868728145222029 1102 58-59 0.8942597209423369 1102 60-61 0.711746946223542 1102 62-63 0.7644738682732637 1102 64-65 0.7898161284572964 1102 66-67 0.7743645912075152 1102 68-69 0.6574288662782166 1102 70-71 0.8508328333295374 1102 72-73 0.8128054769937059 1102 74-75 0.7222616972944031 1102 76-77 1.0425716239433847 1102 78-79 0.9785206491089546 1102 80-81 1.059443131804887 1102 82-83 0.9693629370062951 1102 84-85 0.8785632738576545 1102 86-87 0.9278680924821536 1102 88-89 1.1290988790873193 1102 90-91 1.2009729408144274 1102 92-93 1.0618611442771133 1102 94-95 1.103361967602524 1102 96-97 1.2801490053013218 1102 98-99 1.462107770220502 1102 100-101 1.447240966620619 1103 1 0.24908507960145698 1103 2 0.35051033903289763 1103 3 0.1297551052601058 1103 4 0.17583658649526512 1103 5 0.243619338179208 1103 6 0.21642990560444275 1103 7 0.2734592676031795 1103 8 0.2337395840594283 1103 9 0.15692109829100787 1103 10-11 0.39229478031887766 1103 12-13 0.2548535823755671 1103 14-15 0.4418331955738779 1103 16-17 0.32573293152430693 1103 18-19 0.3876046589595106 1103 20-21 0.36349771304118406 1103 22-23 0.2552170832580174 1103 24-25 0.2897410407837313 1103 26-27 0.06023189359816428 1103 28-29 0.3241711605946236 1103 30-31 0.3287863905052646 1103 32-33 0.3802358604461915 1103 34-35 0.416669842464394 1103 36-37 0.6161621030415105 1103 38-39 0.5004614794927349 1103 40-41 0.6939675455453056 1103 42-43 0.7665975691448708 1103 44-45 0.4479625476659521 1103 46-47 0.50710846040365 1103 48-49 0.32670183333046054 1103 50-51 0.21936221068439465 1103 52-53 0.3228797477847749 1103 54-55 0.4331934909234576 1103 56-57 0.5310851006026027 1103 58-59 0.492761033278299 1103 60-61 0.6100697808692175 1103 62-63 0.5216995900580415 1103 64-65 0.4838187531292064 1103 66-67 0.6560338825460974 1103 68-69 0.7112818846509228 1103 70-71 0.6316228595762574 1103 72-73 0.6161046895921309 1103 74-75 0.8389336133049028 1103 76-77 0.827973198746534 1103 78-79 0.7842844286365143 1103 80-81 0.8590389323298204 1103 82-83 0.8359797349065516 1103 84-85 1.0115895205768126 1103 86-87 0.8456397460254621 1103 88-89 0.9075949420794451 1103 90-91 1.1088364578747942 1103 92-93 1.1849897532009983 1103 94-95 0.9829945135342797 1103 96-97 1.1666739396845216 1103 98-99 1.4825382164147278 1103 100-101 1.5838420164893847 1104 1 0.06159672558485596 1104 2 0.173370273123286 1104 3 0.06107175355939276 1104 4 -0.08550726682124576 1104 5 -0.0237817574749144 1104 6 0.07598999355490577 1104 7 0.13504775311956507 1104 8 0.12662182519916598 1104 9 0.19881602623232197 1104 10-11 0.26353079848394856 1104 12-13 0.17266954123263645 1104 14-15 0.27956471772387204 1104 16-17 0.39392582095268125 1104 18-19 0.3283618741453438 1104 20-21 0.4521070095971709 1104 22-23 0.34668862979137316 1104 24-25 0.267617665232045 1104 26-27 0.2941557781125965 1104 28-29 0.18967802041158421 1104 30-31 0.30528080309517946 1104 32-33 0.37663092947026655 1104 34-35 0.41380160312412784 1104 36-37 0.46286569080297824 1104 38-39 0.45501552484152796 1104 40-41 0.6103374060064581 1104 42-43 0.45350362399145894 1104 44-45 0.45107834733907026 1104 46-47 0.43826938526775905 1104 48-49 0.32263771786619344 1104 50-51 0.4070275836486772 1104 52-53 0.47783920645284894 1104 54-55 0.5190057550091112 1104 56-57 0.4740337571219655 1104 58-59 0.6104267256752678 1104 60-61 0.7612898806386923 1104 62-63 0.6500735978202599 1104 64-65 0.5891636605735897 1104 66-67 0.5374098920864867 1104 68-69 0.6749657155006688 1104 70-71 0.711129655506685 1104 72-73 0.537019811003173 1104 74-75 0.40602978383936517 1104 76-77 0.4912139498392598 1104 78-79 0.6265186207113906 1104 80-81 0.8056324489110374 1104 82-83 0.6997916050252257 1104 84-85 0.5073982975222933 1104 86-87 0.4989221830839128 1104 88-89 0.5352435714470225 1104 90-91 0.6407897089009715 1104 92-93 0.7255244302811654 1104 94-95 0.7642882691576247 1104 96-97 0.7801111418800879 1104 98-99 1.0356412387803395 1104 100-101 1.1850063486354898 1105 1 0.17423563320423696 1105 2 0.27528784733960876 1105 3 0.2631165727971556 1105 4 0.19270108291082266 1105 5 0.2797370673572246 1105 6 0.33469416768701166 1105 7 0.3306651850453193 1105 8 0.29025461092937377 1105 9 0.3166245495387088 1105 10-11 0.3608294334668827 1105 12-13 0.45506449987112774 1105 14-15 0.47523054312388524 1105 16-17 0.5443423717961053 1105 18-19 0.557196036360267 1105 20-21 0.6889561403035884 1105 22-23 0.5918192371271829 1105 24-25 0.6710387398332216 1105 26-27 0.6792756333817067 1105 28-29 0.48811254885731614 1105 30-31 0.5731217110393203 1105 32-33 0.5200054270726184 1105 34-35 0.6082557568438247 1105 36-37 0.6557178141068007 1105 38-39 0.6977970991752827 1105 40-41 0.6137708197968124 1105 42-43 0.6256990730783158 1105 44-45 0.5806010730063207 1105 46-47 0.6534125519715843 1105 48-49 0.8451996271103113 1105 50-51 0.8368483305503531 1105 52-53 0.6922337650997719 1105 54-55 0.8352486232788578 1105 56-57 0.9984988372166086 1105 58-59 0.9961362474009547 1105 60-61 0.7634728620987161 1105 62-63 0.9738442888974177 1105 64-65 1.076277005888322 1105 66-67 0.9238652696335663 1105 68-69 1.0965437464229524 1105 70-71 1.0837867003580364 1105 72-73 1.0414653593998615 1105 74-75 1.1803969302206774 1105 76-77 1.0513133697597574 1105 78-79 1.0535550226955657 1105 80-81 0.9831998029943918 1105 82-83 1.2351413178294486 1105 84-85 1.2524316592036513 1105 86-87 1.3553674140561007 1105 88-89 1.1486565453017015 1105 90-91 1.3188159982545002 1105 92-93 1.538261867931567 1105 94-95 1.428029538837258 1105 96-97 1.251495002587614 1105 98-99 1.5890430936035855 1105 100-101 1.6823811746712707 1106 1 0.04811299961891535 1106 2 0.14441623139336812 1106 3 0.16151419507537312 1106 4 0.03314173678431587 1106 5 0.03618211484705114 1106 6 0.008435848891380715 1106 7 0.06404374678209024 1106 8 0.08327005319854663 1106 9 0.1446273924669228 1106 10-11 -0.20383103044731143 1106 12-13 -0.39797549495991547 1106 14-15 -0.23797292007957083 1106 16-17 -0.19585275645872713 1106 18-19 -0.2521339371488125 1106 20-21 -0.17400179728122112 1106 22-23 -0.18711078807149306 1106 24-25 -0.39730460836086934 1106 26-27 -0.2808264535860445 1106 28-29 0.15119324925145605 1106 30-31 0.1445018234642106 1106 32-33 0.3054935707697126 1106 34-35 -0.054566495530671943 1106 36-37 -0.01829846811843794 1106 38-39 0.004187226460935278 1106 40-41 -0.06352452167763545 1106 42-43 0.058017997934555865 1106 44-45 0.09635024920326174 1106 46-47 0.3454071245631738 1106 48-49 0.5772859487488731 1106 50-51 0.572132946431978 1106 52-53 0.5220134381630217 1106 54-55 0.5456899232724055 1106 56-57 0.5492192610713502 1106 58-59 0.43147578348676063 1106 60-61 0.09351098318160922 1106 62-63 0.4068093338027623 1106 64-65 0.4005161995305002 1106 66-67 0.43147333321178394 1106 68-69 0.45005217828958877 1106 70-71 0.2613566428533467 1106 72-73 0.37067967742014574 1106 74-75 0.3891906240890002 1106 76-77 0.4069539977884453 1106 78-79 0.4646165979362493 1106 80-81 0.4243059605184598 1106 82-83 0.31060813956492694 1106 84-85 0.4317622788327782 1106 86-87 0.38844876057028443 1106 88-89 0.5263298670048719 1106 90-91 0.9985827489167711 1106 92-93 0.9340103980084571 1106 94-95 0.8487990678157402 1106 96-97 0.8688142505038776 1106 98-99 1.1595832499646406 1106 100-101 0.4647356361302144 1107 1 0.380837599086135 1107 2 0.35936710407467487 1107 3 0.3318468285275209 1107 4 0.24523611766073827 1107 5 0.2736778423165731 1107 6 0.37259487925754087 1107 7 0.4051967603402531 1107 8 0.23195140109374535 1107 9 0.41695495856229314 1107 10-11 0.48370999940886605 1107 12-13 0.5180555819347816 1107 14-15 0.543462595906469 1107 16-17 0.511813655212471 1107 18-19 0.4920966547119505 1107 20-21 0.6190596132235129 1107 22-23 0.46310161772937875 1107 24-25 0.4390848728047345 1107 26-27 0.40891404783270247 1107 28-29 0.3615607139996868 1107 30-31 0.47173533963471925 1107 32-33 0.5354015553831744 1107 34-35 0.4349002926657519 1107 36-37 0.4806704278596854 1107 38-39 0.4081982509459223 1107 40-41 0.46763266946588544 1107 42-43 0.5173844698280874 1107 44-45 0.43571775192953766 1107 46-47 0.7304760547215423 1107 48-49 0.5950509948379406 1107 50-51 0.5042683436009838 1107 52-53 0.49264392802560053 1107 54-55 0.5306845249640517 1107 56-57 0.5842472291914973 1107 58-59 0.634939110255317 1107 60-61 0.7471362592006443 1107 62-63 0.6036189064412056 1107 64-65 0.6328059503402486 1107 66-67 0.8141813851006532 1107 68-69 0.8716247950314013 1107 70-71 0.76022977989442 1107 72-73 0.8692202352634268 1107 74-75 1.0500759466582714 1107 76-77 0.6716148809408402 1107 78-79 0.7732102165389811 1107 80-81 1.0916008926191338 1107 82-83 1.0275512321716889 1107 84-85 0.9528609837813207 1107 86-87 1.1074177108027676 1107 88-89 0.9381217798506789 1107 90-91 0.931158691450559 1107 92-93 1.0389247575086635 1107 94-95 0.875423036304813 1107 96-97 0.9497367915608628 1107 98-99 1.224453826200147 1107 100-101 1.2535641218369022 1108 1 0.04074704592443368 1108 2 0.10729575518252688 1108 3 0.010150159746491028 1108 4 0.08319845279492455 1108 5 0.11419819490521377 1108 6 0.06114559121537155 1108 7 0.12175661510522673 1108 8 0.2792238698704068 1108 9 0.05510977384696503 1108 10-11 0.15646913674782326 1108 12-13 0.19247814148332765 1108 14-15 0.3024369829228135 1108 16-17 0.10700055781313722 1108 18-19 0.16153222854340044 1108 20-21 0.20820963837716988 1108 22-23 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0.15707084991217002 2316 80-81 0.39251140489725245 2316 82-83 0.4505838205404302 2316 84-85 0.5130626045136104 2316 86-87 0.43921615138443215 2316 88-89 0.32907746007794714 2316 90-91 0.6423076128358467 2316 92-93 0.6941154950133885 2316 94-95 0.5845266262370608 2316 96-97 0.7312761806694574 2316 98-99 0.7621184797251885 2316 100-101 0.5281178073167325 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5130.0 3 1029.0 4 984.0 5 1089.0 6 1269.0 7 1513.0 8 1802.0 9 2275.0 10 2799.0 11 3655.0 12 4831.0 13 6416.0 14 7507.0 15 9409.0 16 11988.0 17 15595.0 18 13575.0 19 9468.0 20 8912.0 21 9233.0 22 9991.0 23 10852.0 24 12310.0 25 13859.0 26 16298.0 27 19246.0 28 22874.0 29 27346.0 30 33809.0 31 42080.0 32 54005.0 33 70434.0 34 95834.0 35 133832.0 36 184817.0 37 231395.0 38 161387.0 39 17455.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.32218251045611 18.754966700020358 14.90375508067375 22.019095708849783 2 15.315035732019282 19.992803313618392 40.902005644213425 23.7901553101489 3 19.269792811527072 25.112990111548523 29.747057700571688 25.87015937635272 4 12.493528185684308 16.618204987794886 36.99481547767293 33.89345134884788 5 13.564279888934458 37.53451155832748 33.48858624000402 15.41262231273404 6 31.43665864488091 35.97547139441668 17.470350145059534 15.117519815642874 7 28.39501908158723 33.42893099931619 20.091996724608848 18.084053194487733 8 26.823156078286054 34.482496021555434 19.34768425870606 19.346663641452448 9 26.90132175054608 16.887534213662853 20.3142396361849 35.896904399606164 10-11 25.186774152263236 26.942431554094426 26.41467434361105 21.456119950031287 12-13 26.176616532616443 24.43128107206678 27.581670660431577 21.810431734885196 14-15 23.0158861018397 25.33218072138747 26.606256610048924 25.045676566723905 16-17 21.67243874550608 29.071337085086448 26.91358838817103 22.342635781236446 18-19 21.615709124938714 26.72771444149647 30.463750332186724 21.19282610137809 20-21 22.66698956608363 25.662575080983252 30.47768834371047 21.19274700922265 22-23 22.191024357232607 25.737780658117497 30.52597035805929 21.545224626590603 24-25 21.949405258408284 25.97742874896421 30.627690546743043 21.445475445884462 26-27 21.588209525394614 26.325431355954827 30.509807862305244 21.576551256345315 28-29 21.411186005519593 26.351809177596586 30.75130825308465 21.485696563799177 30-31 21.405160443898517 26.025158017837434 30.785537378417242 21.784144159846804 32-33 21.361643623726593 26.118669080296375 30.648975090697594 21.870712205279442 34-35 21.941945454966564 26.253305869638528 30.21673785793387 21.588010817461036 36-37 22.033424631295183 25.91704210304031 30.250555206221136 21.79897805944337 38-39 21.60380350145685 26.19287212398272 30.40552314628755 21.797801228272885 40-41 21.728923180772824 26.165671138042274 30.39967661672017 21.70572906446473 42-43 21.844658669848645 26.117958299315458 30.021195754568865 22.016187276267036 44-45 21.703716932956258 26.496458521822923 29.778287176689243 22.021537368531575 46-47 21.959267047066984 26.719190537852867 29.317214533924567 22.004327881155582 48-49 22.126066199260457 26.775474246922858 29.020641484205118 22.077818069611567 50-51 22.062698341663488 27.026716160812004 28.768079416747927 22.14250608077658 52-53 22.300086340697487 27.072108028349902 28.62855713661423 21.99924849433838 54-55 21.891807234779822 26.967449849625417 28.708670541105135 22.432072374489625 56-57 22.034250182687 27.37612800884119 28.59430299120041 21.995318817271396 58-59 22.21324352719596 26.878301354745098 28.70047060310274 22.2079845149562 60-61 22.180494795713507 26.815574716161933 29.109942219940947 21.89398826818361 62-63 21.937497252868006 27.013255304319433 29.06453876438712 21.984708678425438 64-65 21.919683345374803 26.91222888547289 28.988057558869436 22.18003021028288 66-67 21.853827993042866 27.29207087736483 28.613408179026877 22.240692950565425 68-69 21.793964130136178 27.144813782818574 28.804010831600014 22.257211255445235 70-71 21.834453078058484 27.23108760381955 28.67199282902893 22.262466489093036 72-73 22.041187147412312 27.147608535688008 28.617041942604853 22.194162374294823 74-75 21.813423995767273 27.48541271058645 28.409320165195705 22.291843128450573 76-77 21.98985859331154 27.32065134204877 28.373097014486042 22.316393050153653 78-79 22.116312239340687 27.336288486129177 28.31765877797341 22.229740496556726 80-81 21.882337704641383 27.718828675205202 28.254217432512252 22.144616187641162 82-83 21.980405636597073 27.53636666133017 28.294816377397108 22.18841132467565 84-85 21.974377084559546 27.413498136158527 28.182970374730232 22.429154404551696 86-87 22.101891008146588 27.22767634491681 28.24551910216059 22.424913544776018 88-89 22.057516984164856 27.27206371730313 28.1863753092311 22.48404398930092 90-91 21.959900302146814 27.442497328400506 28.297566146370297 22.300036223082383 92-93 22.13016293225473 27.16646842503268 28.25867452674143 22.44469411597116 94-95 21.942552480099707 27.11654953842 28.23047134373857 22.71042663774172 96-97 22.126713862264673 27.04544018548584 28.139188161934353 22.68865779031513 98-99 22.117325132050233 27.181429054740896 27.88149742493212 22.81974838827675 100-101 22.312630085295144 26.541442074960525 28.42338191943894 22.722545920305393 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1980.0 1 1218.0 2 662.5 3 818.0 4 594.0 5 511.5 6 938.0 7 2045.5 8 3456.0 9 3973.5 10 3100.0 11 2242.0 12 2145.0 13 2265.5 14 2409.5 15 2534.0 16 2590.5 17 2596.0 18 2728.0 19 2926.0 20 3159.5 21 3478.0 22 4025.5 23 4754.5 24 5669.0 25 6854.0 26 8323.0 27 9933.0 28 11056.5 29 12323.0 30 14433.5 31 16814.0 32 19275.5 33 21522.5 34 23956.0 35 25953.0 36 28322.0 37 30923.0 38 32567.5 39 33627.5 40 34957.0 41 38434.0 42 41578.0 43 44069.5 44 46462.0 45 47780.5 46 47491.5 47 47368.0 48 49993.0 49 50198.0 50 47125.5 51 44754.0 52 42915.5 53 41794.5 54 40699.0 55 37635.5 56 33627.0 57 30274.0 58 27322.5 59 24053.5 60 20560.0 61 17184.5 62 14064.0 63 11109.0 64 8638.5 65 6792.5 66 5241.0 67 4187.5 68 3025.5 69 2045.0 70 1627.5 71 1264.5 72 1011.0 73 860.5 74 687.0 75 550.0 76 407.0 77 272.0 78 173.0 79 107.5 80 72.0 81 54.0 82 35.0 83 22.0 84 16.5 85 12.0 86 10.0 87 8.0 88 4.0 89 4.5 90 5.5 91 3.5 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3197514071882561 2 0.27391593225438454 3 0.26678596948689337 4 0.2724272587095238 5 0.2775200892577317 6 0.21068648221740277 7 0.20081422607779964 8 0.20097092855620602 9 0.21076483345660596 10-11 0.21209680452306034 12-13 0.20778748636688438 14-15 0.20958956486855795 16-17 0.19583892238839648 18-19 0.19959978187015004 20-21 0.19035433564417253 22-23 0.19858121576050847 24-25 0.1970925422156477 26-27 0.19822863518409406 28-29 0.20833594504130679 30-31 0.2064163396808284 32-33 0.21182257518584913 34-35 0.20547612481039 36-37 0.19219558976544773 38-39 0.18177487495142222 40-41 0.17817071794807507 42-43 0.19305745339668293 44-45 0.19364508769070693 46-47 0.1935667364515037 48-49 0.20994214544497236 50-51 0.2051235442339756 52-53 0.2241237197407514 54-55 0.1947028294199501 56-57 0.1776222592736527 58-59 0.18024702578695986 60-61 0.16939537915731676 62-63 0.17613358572879187 64-65 0.1750758439995487 66-67 0.1736263460742895 68-69 0.1766036931640111 70-71 0.17852329852448948 72-73 0.17562430267397108 74-75 0.18968835011094534 76-77 0.18169652371221903 78-79 0.1857707881507854 80-81 0.17836659604608307 82-83 0.16140355275859042 84-85 0.16093344532337123 86-87 0.1637149143150848 88-89 0.17049229650616154 90-91 0.17664286878361268 92-93 0.17123663327859193 94-95 0.17252942872544472 96-97 0.1758593563915807 98-99 0.18467387080194061 100-101 0.16767165189484637 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1276304.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.89807102241649 #Duplication Level Percentage of deduplicated Percentage of total 1 79.3142938873272 45.12830326693956 2 11.80718787624852 13.436124287156066 3 3.6875468311488864 6.294429044915885 4 1.6161266401778311 3.678179534162303 5 0.8932783561593991 2.541290767577247 6 0.5417099329629669 1.8493350143565217 7 0.37919843342713794 1.5102961577708451 8 0.2561691135271597 1.1660422732174243 9 0.20256100385508646 1.037279734534682 >10 1.1596957762605873 12.2364521181597 >50 0.09284515644027413 3.6403953048142155 >100 0.046765903559266624 4.714713622507229 >500 0.0017933766197434542 0.7565064170458806 >1k 5.518081906902936E-4 0.7557214965052644 >5k 2.759040953451468E-4 1.254930960337159 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8979 0.7035157768055259 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7009 0.5491638355752234 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4887 0.3829025059860347 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1733 0.1357826975391443 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1686 0.1321001892965939 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1322 0.1035803382266294 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 7.835123920319924E-5 0.0 0.0 0.0 0.0 14-15 7.835123920319924E-5 0.0 0.0 7.835123920319924E-5 3.917561960159962E-5 16-17 7.835123920319924E-5 0.0 0.0 1.958780980079981E-4 7.835123920319924E-5 18-19 7.835123920319924E-5 0.0 0.0 2.350537176095977E-4 7.835123920319924E-5 20-21 7.835123920319924E-5 0.0 0.0 2.350537176095977E-4 7.835123920319924E-5 22-23 7.835123920319924E-5 0.0 0.0 3.1340495681279694E-4 7.835123920319924E-5 24-25 1.5670247840639847E-4 0.0 0.0 5.484586744223947E-4 7.835123920319924E-5 26-27 1.5670247840639847E-4 0.0 0.0 8.22688011633592E-4 7.835123920319924E-5 28-29 1.958780980079981E-4 0.0 0.0 0.0014886735448607856 7.835123920319924E-5 30-31 2.350537176095977E-4 0.0 0.0 0.003800035101355163 7.835123920319924E-5 32-33 2.350537176095977E-4 0.0 0.0 0.008383582594742318 7.835123920319924E-5 34-35 2.350537176095977E-4 0.0 0.0 0.013163008186137472 7.835123920319924E-5 36-37 2.350537176095977E-4 0.0 0.0 0.019156877985182213 7.835123920319924E-5 38-39 3.1340495681279694E-4 0.0 0.0 0.02585590893705575 7.835123920319924E-5 40-41 3.1340495681279694E-4 0.0 0.0 0.03459207210821247 7.835123920319924E-5 42-43 3.1340495681279694E-4 0.0 0.0 0.045287016259449156 7.835123920319924E-5 44-45 3.1340495681279694E-4 0.0 0.0 0.05762733643395304 7.835123920319924E-5 46-47 3.917561960159962E-4 0.0 0.0 0.07462955534104727 7.835123920319924E-5 48-49 3.917561960159962E-4 0.0 0.0 0.09539263372989507 7.835123920319924E-5 50-51 3.917561960159962E-4 0.0 0.0 0.11842789805563565 7.835123920319924E-5 52-53 3.917561960159962E-4 0.0 0.0 0.14647764169038097 7.835123920319924E-5 54-55 3.917561960159962E-4 0.0 0.0 0.1766036931640111 7.835123920319924E-5 56-57 3.917561960159962E-4 0.0 0.0 0.21765974250648748 7.835123920319924E-5 58-59 3.917561960159962E-4 0.0 0.0 0.26043951911143426 7.835123920319924E-5 60-61 3.917561960159962E-4 0.0 0.0 0.31391423986761774 7.835123920319924E-5 62-63 3.917561960159962E-4 0.0 0.0 0.3667621507101756 7.835123920319924E-5 64-65 3.917561960159962E-4 0.0 0.0 0.42799364414747587 7.835123920319924E-5 66-67 3.917561960159962E-4 0.0 0.0 0.49792212513633116 7.835123920319924E-5 68-69 4.701074352191954E-4 0.0 0.0 0.583560029585428 7.835123920319924E-5 70-71 5.092830548207951E-4 0.0 0.0 0.6880805826824956 7.835123920319924E-5 72-73 5.484586744223947E-4 0.0 0.0 0.7877041833293635 7.835123920319924E-5 74-75 5.484586744223947E-4 0.0 0.0 0.9014310070328073 7.835123920319924E-5 76-77 5.484586744223947E-4 0.0 0.0 1.0176650703907533 7.835123920319924E-5 78-79 5.876342940239942E-4 0.0 0.0 1.1483549373816897 7.835123920319924E-5 80-81 7.835123920319924E-4 0.0 0.0 1.2972222918677683 1.1752685880479885E-4 82-83 8.618636312351917E-4 0.0 0.0 1.4646980656646065 1.5670247840639847E-4 84-85 9.010392508367912E-4 0.0 0.0 1.63891204603292 1.5670247840639847E-4 86-87 9.402148704383908E-4 0.0 0.0 1.817592047035816 1.5670247840639847E-4 88-89 9.402148704383908E-4 0.0 0.0 2.0325094961701913 1.5670247840639847E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4330 0.0 47.884155 1 GTATCAA 7285 0.0 38.70957 1 ATCAACG 9375 0.0 29.914812 3 TATCAAC 9410 0.0 29.757708 2 TCAACGC 9570 0.0 29.306414 4 CAACGCA 9630 0.0 29.023956 5 AACGCAG 9815 0.0 28.507406 6 GTACATG 7250 0.0 27.745693 1 TACATGG 7495 0.0 26.133608 2 ACATGGG 7390 0.0 25.085672 3 ACGCAGA 11155 0.0 24.959103 7 CGCAGAG 11185 0.0 24.849682 8 GTGTAGC 615 0.0 24.743895 1 TGGTATC 2185 0.0 23.933987 2 GCAGAGT 11685 0.0 23.297523 9 GAGTACT 6605 0.0 23.231163 12-13 GTGGTAT 2285 0.0 23.100958 1 CATGGGA 4285 0.0 21.74342 4 GTACTTT 7120 0.0 21.246822 14-15 AGAGTAC 10675 0.0 21.027308 10-11 >>END_MODULE