FastQCFastQC Report
Thu 26 May 2016
SRR937566_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937566_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276304
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89560.7017136983038524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77110.6041664054958693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55330.4335174065113014No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21040.1648510072835312No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14720.11533302410710927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46600.047.4070631
GTATCAA76750.037.5120051
TATCAAC101400.028.249082
ATCAACG102950.027.6391963
TCAACGC104550.027.2616524
CAACGCA106100.026.8186195
AACGCAG109450.026.0815036
ACGCAGA125850.022.6853877
GTACATG72150.022.651561
CGCAGAG126450.022.5026138
TACATGG74150.021.7176822
GGACCGA1754.051417E-621.71336
GAGTACT77950.021.66448812-13
AGTACTT79450.021.52452312-13
GCAGAGT131100.021.4508259
ACATGGG73700.021.0768263
AGAGTAC119000.020.63804610-11
GTACTTT81350.020.6106214-15
TGGTATC23750.019.801432
GGTATAC1200.004758557319.7954221
Produced by FastQC (version 0.11.5)