FastQCFastQC Report
Thu 26 May 2016
SRR937565_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937565_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228670
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88300.718663270039962No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69100.5623967379361423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47420.38594577876891273No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17570.14300015463875573No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16940.13787265905409915No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12860.10466602098203749No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG451.5100188E-552.8132062
GGTATCA40350.046.431751
GTATCAA71350.041.586561
TATCAAC90900.032.36772
ATCAACG92300.031.923033
TCAACGC92750.031.6630884
CAACGCA94450.031.1434945
AACGCAG96250.030.60176
GTACATG69350.027.9753861
ACGCAGA110550.026.5154347
CGCAGAG110650.026.491478
TACATGG72300.026.2970332
GCAGAGT116900.024.6656959
ACATGGG74100.024.4354823
GAGTACT67150.023.90666412-13
CATGGGG29900.022.7270744
GTACTTT73100.021.82993714-15
AGAGTAC105050.021.54344410-11
AGTACTT69850.021.35067212-13
TTACGCG804.9993992E-520.77738882-83
Produced by FastQC (version 0.11.5)