##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937565_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1228670 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.847170517714275 33.0 31.0 34.0 30.0 34.0 2 31.98016391708107 33.0 31.0 34.0 30.0 34.0 3 31.99133046302099 34.0 31.0 34.0 30.0 34.0 4 35.60219505644314 37.0 35.0 37.0 33.0 37.0 5 35.59679979164462 37.0 35.0 37.0 33.0 37.0 6 35.65923234066104 37.0 35.0 37.0 33.0 37.0 7 35.61018988011427 37.0 35.0 37.0 33.0 37.0 8 35.594740654528884 37.0 35.0 37.0 33.0 37.0 9 37.2359469996012 39.0 37.0 39.0 34.0 39.0 10-11 37.12132793996761 39.0 37.0 39.0 34.0 39.0 12-13 36.99291021999397 39.0 37.0 39.0 33.0 39.0 14-15 38.1430579407001 40.0 38.0 41.0 33.0 41.0 16-17 38.077823174652266 40.0 38.0 41.0 33.0 41.0 18-19 38.035208396070544 40.0 38.0 41.0 33.0 41.0 20-21 37.970454230997746 40.0 38.0 41.0 33.0 41.0 22-23 37.767532372402684 40.0 37.5 41.0 33.0 41.0 24-25 37.592397470435515 40.0 37.0 41.0 32.0 41.0 26-27 37.52285072476744 40.0 37.0 41.0 32.0 41.0 28-29 37.561211716734356 40.0 37.0 41.0 32.5 41.0 30-31 37.35128553639301 39.5 37.0 41.0 32.0 41.0 32-33 37.09197343468954 39.0 36.0 41.0 31.5 41.0 34-35 37.08269958573091 39.0 36.0 41.0 31.5 41.0 36-37 36.97425956522093 39.5 36.0 41.0 31.0 41.0 38-39 36.82979278406732 39.0 36.0 41.0 31.0 41.0 40-41 36.58090455533219 39.0 35.5 41.0 30.5 41.0 42-43 36.29690478322088 39.0 35.0 41.0 30.0 41.0 44-45 36.017089617228386 39.0 35.0 41.0 29.5 41.0 46-47 35.61143228043332 39.0 35.0 40.5 27.5 41.0 48-49 35.198191540446174 39.0 35.0 40.0 26.0 41.0 50-51 34.24925854786069 38.0 34.0 39.5 24.5 40.5 52-53 34.34779843245135 38.0 34.0 39.5 24.0 40.5 54-55 34.690983339708794 38.0 34.0 40.0 24.5 41.0 56-57 34.47555283355173 38.0 34.0 40.0 23.5 41.0 58-59 34.221841096470165 38.0 34.0 40.0 23.0 41.0 60-61 33.89621908242246 38.0 34.0 40.0 22.0 41.0 62-63 33.52970732580758 37.0 33.0 40.0 21.5 41.0 64-65 33.145106090325314 36.5 33.0 39.5 20.0 41.0 66-67 32.732661739930165 36.0 32.5 39.0 18.5 41.0 68-69 32.24214272343266 35.5 32.0 39.0 15.0 40.0 70-71 31.79808410720535 35.0 31.0 38.0 11.5 40.0 72-73 31.344311735453783 35.0 31.0 37.5 7.5 40.0 74-75 30.891778101524412 35.0 30.5 37.0 6.5 39.0 76-77 30.3992308756623 34.5 30.0 36.5 2.0 39.0 78-79 29.87619254966753 34.0 29.5 36.0 2.0 38.5 80-81 29.349022520286162 34.0 29.0 35.5 2.0 37.0 82-83 28.822230948912235 34.0 29.0 35.0 2.0 37.0 84-85 28.36538452147444 34.0 27.5 35.0 2.0 36.0 86-87 27.892620475799035 33.5 27.0 35.0 2.0 36.0 88-89 27.406761376122148 33.0 26.0 35.0 2.0 35.5 90-91 26.896662244540845 33.0 25.5 35.0 2.0 35.0 92-93 26.311477451227752 32.5 24.5 35.0 2.0 35.0 94-95 25.668810990746092 32.0 22.5 34.5 2.0 35.0 96-97 24.925083627011322 32.0 19.0 34.0 2.0 35.0 98-99 23.958012322267166 31.5 2.0 34.0 2.0 35.0 100-101 21.59277063816973 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18549998920428834 1101 2 0.24486860795814636 1101 3 0.23438927032234247 1101 4 0.2046860996040749 1101 5 0.13521269917382028 1101 6 0.21305718109971394 1101 7 0.28561137702277506 1101 8 0.17235136116186567 1101 9 0.3911682949000195 1101 10-11 0.2848285804520927 1101 12-13 0.3560670661338179 1101 14-15 0.41231430618918097 1101 16-17 0.502886344775952 1101 18-19 0.5359762389658798 1101 20-21 0.5894373887422688 1101 22-23 0.45023219374805024 1101 24-25 0.6051098176637666 1101 26-27 0.6557859960177481 1101 28-29 0.42968004936174253 1101 30-31 0.48322810760071633 1101 32-33 0.43246398603145764 1101 34-35 0.5024184817307713 1101 36-37 0.3248752326428743 1101 38-39 0.356116699303648 1101 40-41 0.28183888627089715 1101 42-43 0.3658477419576087 1101 44-45 0.36580927881514214 1101 46-47 0.2965616343971078 1101 48-49 0.3677633027998439 1101 50-51 0.26693788895894244 1101 52-53 0.2117840672800142 1101 54-55 0.3508401813255091 1101 56-57 0.24964320880541635 1101 58-59 0.49309399405836274 1101 60-61 0.35082049096636325 1101 62-63 0.4374914541778949 1101 64-65 0.45697085214073496 1101 66-67 0.5851132250472588 1101 68-69 0.4922096060850052 1101 70-71 0.38687681494394965 1101 72-73 0.3681536244415433 1101 74-75 0.3718100864675371 1101 76-77 0.5714565099307407 1101 78-79 0.6192686484139607 1101 80-81 0.6542894337977039 1101 82-83 0.6582111240994806 1101 84-85 0.7021092467427863 1101 86-87 0.9708634561021583 1101 88-89 1.0657047793738244 1101 90-91 1.0864927346352289 1101 92-93 1.1488738085072896 1101 94-95 1.2793115755080535 1101 96-97 1.1442206860316375 1101 98-99 1.294650697766965 1101 100-101 1.7676200690611985 1102 1 0.2502181411571449 1102 2 0.11331344639307872 1102 3 0.3260555024406777 1102 4 0.2746996575119809 1102 5 0.010559903289866668 1102 6 -0.01425505056669607 1102 7 0.16050491268180878 1102 8 0.2598685258209201 1102 9 0.26737069013042003 1102 10-11 0.1619304907265473 1102 12-13 0.1685199176399621 1102 14-15 0.2976170125561808 1102 16-17 0.3395352818707451 1102 18-19 0.3348159949235381 1102 20-21 0.41543250441904434 1102 22-23 0.48089218575236004 1102 24-25 0.39909653786678945 1102 26-27 0.4101466885139686 1102 28-29 0.2535537000891779 1102 30-31 0.3489796154834153 1102 32-33 0.36180295111115157 1102 34-35 0.3710071556630936 1102 36-37 0.39651052492008176 1102 38-39 0.6532743186740859 1102 40-41 0.5679103086344952 1102 42-43 0.684433808599934 1102 44-45 0.754900377527143 1102 46-47 0.7101443115624662 1102 48-49 0.7241177030362422 1102 50-51 0.9053532650170695 1102 52-53 0.8218903578016494 1102 54-55 0.9796776776318481 1102 56-57 1.0106568362410613 1102 58-59 0.8268822994936826 1102 60-61 1.1103914179448608 1102 62-63 1.1196241304741434 1102 64-65 0.9671353721386495 1102 66-67 1.0582094688114907 1102 68-69 0.959672799841421 1102 70-71 1.0946395863193779 1102 72-73 1.14629453977415 1102 74-75 1.1482141121232736 1102 76-77 1.1562594857177082 1102 78-79 1.1453137371487294 1102 80-81 1.3482214009110898 1102 82-83 1.4564629361660195 1102 84-85 1.4574433274992487 1102 86-87 1.4925600196939648 1102 88-89 1.3645879901645301 1102 90-91 1.6316762009403512 1102 92-93 1.7537472680334574 1102 94-95 1.6899262875763341 1102 96-97 1.908497336880746 1102 98-99 2.0454111573452742 1102 100-101 2.661391687840638 1103 1 0.2405419331774361 1103 2 0.22031855796856803 1103 3 0.19056959351242497 1103 4 0.10671658712068677 1103 5 0.08414803489194611 1103 6 0.1783656369556752 1103 7 0.19260799243917148 1103 8 0.16576473035462413 1103 9 0.17262452381203275 1103 10-11 0.2009677738601212 1103 12-13 0.17106731126661856 1103 14-15 0.37439269965883426 1103 16-17 0.28981560120062255 1103 18-19 0.18336977691506462 1103 20-21 0.31221998746512725 1103 22-23 0.2608399655084028 1103 24-25 0.19545570004554946 1103 26-27 0.22271709969316333 1103 28-29 0.10299703759011436 1103 30-31 0.21722312440106606 1103 32-33 0.09874369306274389 1103 34-35 0.28669001708504993 1103 36-37 0.4690776668115859 1103 38-39 0.4529526914524027 1103 40-41 0.46875855625210505 1103 42-43 0.360516760148208 1103 44-45 0.28128573818700886 1103 46-47 0.33302623284331645 1103 48-49 0.4834298714133922 1103 50-51 0.34221094938943253 1103 52-53 0.3780145288825949 1103 54-55 0.4831243462718646 1103 56-57 0.4427130856612038 1103 58-59 0.2830972595774526 1103 60-61 0.5359344952281759 1103 62-63 0.7903699698637681 1103 64-65 0.7860097652218982 1103 66-67 0.647577877772882 1103 68-69 0.6545433316308191 1103 70-71 0.8089460334430747 1103 72-73 0.7928805640110603 1103 74-75 0.7041865264038769 1103 76-77 0.8323119533672241 1103 78-79 0.8901255101187893 1103 80-81 0.7176855911659601 1103 82-83 0.8317080222940803 1103 84-85 0.9740935004622386 1103 86-87 0.9853514612289338 1103 88-89 0.8302783621122742 1103 90-91 0.9329463633886057 1103 92-93 1.227115164511904 1103 94-95 1.2550909227194076 1103 96-97 1.7879328545989601 1103 98-99 1.5218358378165853 1103 100-101 1.5839203935119706 1104 1 0.09280279438863204 1104 2 0.11726645593913076 1104 3 0.21121143908556306 1104 4 0.1065019981214661 1104 5 0.10663274205877116 1104 6 0.09254305626706838 1104 7 0.09100414168896265 1104 8 -0.015029195070759727 1104 9 0.15960193450395366 1104 10-11 0.3079543068291528 1104 12-13 0.2932687878903337 1104 14-15 0.42205614868766617 1104 16-17 0.5556606194586422 1104 18-19 0.40621394310212366 1104 20-21 0.4162783682429847 1104 22-23 0.4530811815007567 1104 24-25 0.4292762282218021 1104 26-27 0.4779940586707525 1104 28-29 0.3444978766420803 1104 30-31 0.4958613282462494 1104 32-33 0.3763435195958209 1104 34-35 0.37367488003091864 1104 36-37 0.4888068357416131 1104 38-39 0.6524012695533727 1104 40-41 0.6556024997694081 1104 42-43 0.6969604368209446 1104 44-45 0.8964787291177032 1104 46-47 0.7840339481708085 1104 48-49 0.7318574907235273 1104 50-51 0.7561358450354376 1104 52-53 0.8591547147579632 1104 54-55 0.950710184116204 1104 56-57 0.9151383992619273 1104 58-59 0.9235291491188846 1104 60-61 1.0983296137324814 1104 62-63 1.1428256671119073 1104 64-65 0.9634234649786677 1104 66-67 0.9355872909572582 1104 68-69 1.0524883052651077 1104 70-71 1.144492072659883 1104 72-73 0.9849449012049014 1104 74-75 1.0517785056237585 1104 76-77 1.1308198415331319 1104 78-79 1.1141959312250087 1104 80-81 1.1983586643767232 1104 82-83 1.4075712940849634 1104 84-85 1.3964502174121662 1104 86-87 1.5749755892927801 1104 88-89 1.59165741517322 1104 90-91 1.4066055637348214 1104 92-93 1.5532017794557404 1104 94-95 1.7597514994879475 1104 96-97 2.0177639577929263 1104 98-99 2.0188208174844995 1104 100-101 2.5425487118973997 1105 1 0.22545057714180317 1105 2 0.030768012042074133 1105 3 0.2078688039340939 1105 4 0.09582136650074347 1105 5 0.10511426022083015 1105 6 0.04751576685825398 1105 7 0.07897761504805345 1105 8 0.05455338936746301 1105 9 -0.017931117987828316 1105 10-11 0.14101911952778323 1105 12-13 0.11434069781036271 1105 14-15 0.20491982955535804 1105 16-17 0.3252188912579612 1105 18-19 0.24781945196048838 1105 20-21 0.23666181194722924 1105 22-23 0.18451305015043573 1105 24-25 0.2286275933506161 1105 26-27 0.29242231663490514 1105 28-29 0.21911684539116294 1105 30-31 0.3232991993431824 1105 32-33 0.38869194181371824 1105 34-35 0.18722468520061852 1105 36-37 0.16313739169689256 1105 38-39 0.24082991125278852 1105 40-41 0.3765533637851064 1105 42-43 0.42751996610568455 1105 44-45 0.33955705719818496 1105 46-47 0.2138480152661728 1105 48-49 0.3722980150488624 1105 50-51 0.174054353960571 1105 52-53 0.17935052320257938 1105 54-55 0.3380194568720398 1105 56-57 0.28796025486648347 1105 58-59 0.16821587347752143 1105 60-61 0.18133587858694256 1105 62-63 0.3426971910393988 1105 64-65 0.2658409501394914 1105 66-67 0.35981650506179363 1105 68-69 0.42192191012876634 1105 70-71 0.5752878154556775 1105 72-73 0.5040140986432782 1105 74-75 0.5327074271301449 1105 76-77 0.6078387546029873 1105 78-79 0.691521409271683 1105 80-81 0.582280156655461 1105 82-83 0.6139115318315334 1105 84-85 0.5312779080500576 1105 86-87 0.6455588951156805 1105 88-89 0.6715582929676458 1105 90-91 0.8836432873570175 1105 92-93 1.1795905860484623 1105 94-95 1.1030424338363005 1105 96-97 1.1846953829838291 1105 98-99 1.3331747737668955 1105 100-101 1.6493385688713609 1106 1 0.16805422688660343 1106 2 0.20769129309719148 1106 3 0.09894789353241507 1106 4 0.18324186557636324 1106 5 0.1838854125530034 1106 6 0.10058832831390419 1106 7 0.02800148965109628 1106 8 -0.025288095298478197 1106 9 0.20070681777453814 1106 10-11 0.044936889698242055 1106 12-13 0.05962283210610764 1106 14-15 0.025249951891687772 1106 16-17 -0.25219355821085543 1106 18-19 0.1178377626045446 1106 20-21 0.15493707311716776 1106 22-23 0.18112690785815033 1106 24-25 0.09244328389188894 1106 26-27 0.31221727280435374 1106 28-29 0.318268540673742 1106 30-31 0.40782360911239124 1106 32-33 0.4215732587647736 1106 34-35 0.29791916281652675 1106 36-37 0.30060698452832213 1106 38-39 0.2356533948749373 1106 40-41 0.3058654320033085 1106 42-43 0.28625290491983435 1106 44-45 0.3218576525152699 1106 46-47 0.12955359426288737 1106 48-49 0.23956884451219196 1106 50-51 0.33474662014602785 1106 52-53 0.4333819608977407 1106 54-55 0.5376470519856582 1106 56-57 0.29562064991640824 1106 58-59 0.23513110492583422 1106 60-61 0.07720758023842933 1106 62-63 0.3894441768597048 1106 64-65 0.39437990227528985 1106 66-67 0.29196893453926975 1106 68-69 0.14011402151409413 1106 70-71 0.40593558766064675 1106 72-73 0.490640251904253 1106 74-75 0.6328984947203047 1106 76-77 0.6628847965847804 1106 78-79 0.8298304610987692 1106 80-81 0.6464735595161706 1106 82-83 0.4673202307143214 1106 84-85 0.5018658831758884 1106 86-87 0.676002331147842 1106 88-89 0.6547841507815129 1106 90-91 1.0110234499413835 1106 92-93 0.8413195708249717 1106 94-95 0.7341979504706799 1106 96-97 0.7906989743468635 1106 98-99 1.1408997425857272 1106 100-101 1.8265564124613114 1107 1 -0.048448692355865575 1107 2 -0.06555225513163876 1107 3 0.032747324402787825 1107 4 0.007978765782425512 1107 5 0.0424278764917716 1107 6 -0.051983180860858624 1107 7 0.04491408409717934 1107 8 0.06283806994536434 1107 9 0.017814437242691383 1107 10-11 0.14169812510203172 1107 12-13 0.02794015866876265 1107 14-15 0.1375763918877695 1107 16-17 0.06218371094821151 1107 18-19 0.16978586632351522 1107 20-21 0.18403811815690574 1107 22-23 0.22805066411367392 1107 24-25 0.3447875705173331 1107 26-27 0.263163880104905 1107 28-29 0.13204110960712256 1107 30-31 0.22778515606558614 1107 32-33 0.11351007801705748 1107 34-35 0.18863094281848447 1107 36-37 0.19878797756139477 1107 38-39 0.32288293564620574 1107 40-41 0.19983314861601542 1107 42-43 0.3102317833419619 1107 44-45 0.25192217022696894 1107 46-47 0.19146698598106582 1107 48-49 0.3124830580339264 1107 50-51 0.19769309252361467 1107 52-53 0.10145151982228384 1107 54-55 0.1371049069557344 1107 56-57 0.29778095067198507 1107 58-59 0.47715807725435155 1107 60-61 0.4991754931027259 1107 62-63 0.5967008862110958 1107 64-65 0.5408354144919514 1107 66-67 0.518540787154329 1107 68-69 0.6708160502649534 1107 70-71 0.8112343807133406 1107 72-73 0.6419769092489673 1107 74-75 0.6331146781174191 1107 76-77 0.6070030749676683 1107 78-79 0.8280807554761616 1107 80-81 0.7127360705553194 1107 82-83 0.7611655967235436 1107 84-85 0.9214333937244135 1107 86-87 0.9972208082354683 1107 88-89 0.8723088048880783 1107 90-91 0.9791215266757582 1107 92-93 0.9461703582844976 1107 94-95 1.2001574981107552 1107 96-97 1.4914251052466803 1107 98-99 1.497513421321866 1107 100-101 2.190537498713887 1108 1 0.13976446782670138 1108 2 -0.06473512507630019 1108 3 0.10567043476341453 1108 4 0.10895963532583863 1108 5 0.028710645287851833 1108 6 0.06187532397922979 1108 7 0.039635653822820416 1108 8 0.08639429936037146 1108 9 0.16587955268582277 1108 10-11 0.15706215549676017 1108 12-13 0.14067249737439624 1108 14-15 0.17735192145492107 1108 16-17 0.2927706025938406 1108 18-19 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-0.10805572639535654 2316 66-67 -0.012176328360332889 2316 68-69 -0.020587328590188747 2316 70-71 -0.010760150789320022 2316 72-73 -0.014074177835379231 2316 74-75 -0.172323224302243 2316 76-77 -0.08336761050552965 2316 78-79 -0.1570104228968141 2316 80-81 -0.08914390915832016 2316 82-83 -0.0413506286902674 2316 84-85 0.23330128247283355 2316 86-87 -0.005169821817748499 2316 88-89 0.21555164586064635 2316 90-91 0.3269131798671836 2316 92-93 0.14472817073855992 2316 94-95 0.14139228333992904 2316 96-97 0.570247908292572 2316 98-99 0.6001138976103455 2316 100-101 0.8989403520346748 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5171.0 3 942.0 4 848.0 5 984.0 6 1146.0 7 1390.0 8 1640.0 9 2027.0 10 2541.0 11 3185.0 12 4355.0 13 5963.0 14 6816.0 15 8727.0 16 11181.0 17 15011.0 18 13016.0 19 8676.0 20 8268.0 21 8440.0 22 9125.0 23 10011.0 24 11364.0 25 12574.0 26 14612.0 27 17403.0 28 20950.0 29 25000.0 30 30609.0 31 39003.0 32 50150.0 33 65747.0 34 89869.0 35 128585.0 36 178409.0 37 229744.0 38 166332.0 39 18854.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.25496376518764 18.83622452603298 14.932889878335606 21.975921830443774 2 15.356524265917034 19.974386475275356 40.938215738033556 23.730873520774054 3 19.287773283581604 25.048968239200054 29.730168679261297 25.93308979795704 4 12.564781954813592 16.520204299294058 36.99436210328065 33.920651642611695 5 13.586990670434506 37.54576856508459 33.50915860914155 15.358082155339348 6 31.412656385861364 35.9474181344792 17.515542743973363 15.124382735686076 7 28.46705505545673 33.426148630077215 20.036522574588673 18.070273739877383 8 26.85243644396154 34.43625471684261 19.34692632344856 19.36438251574728 9 26.99332106523626 16.78249577468465 20.263160470625085 35.961022689454005 10-11 25.190789567600746 26.83265457615574 26.555346410880237 21.421209445363274 12-13 26.20692327300535 24.36616874840683 27.644965587560538 21.781942391027275 14-15 22.9778957439988 25.268788699169555 26.67931250779999 25.074003049031656 16-17 21.670101982804116 29.024805239190172 26.932946489819255 22.372146288186453 18-19 21.586294566334054 26.675505742068385 30.496971644675696 21.24122804692187 20-21 22.551160051934836 25.674712433695007 30.55302278999667 21.22110472437349 22-23 22.12415505069492 25.70028142550868 30.62017179106827 21.555391732728133 24-25 21.864295305133115 25.903011922183484 30.735861189070523 21.49683158361288 26-27 21.551690740089008 26.298829068846086 30.51822665080576 21.631253540259145 28-29 21.405355713178096 26.264056228585037 30.857976979822165 21.4726110784147 30-31 21.431714639128842 25.96711986189216 30.842085043334066 21.75908045564493 32-33 21.32794704525665 26.102805777548475 30.72019511359535 21.84905206359953 34-35 21.99960686149045 26.15443655176745 30.316403092580856 21.52955349416124 36-37 22.0233896190216 25.924432283872335 30.34411609993598 21.708061997170084 38-39 21.592534160188848 26.13313064281791 30.483623212576706 21.790711984416536 40-41 21.714264977453645 26.073933446768464 30.515599777044105 21.69620179873378 42-43 21.844166277554734 26.03560527126029 30.081866432315934 22.03836201886904 44-45 21.627328397240607 26.415205133840914 29.86208328015147 22.095383188767013 46-47 21.929503702444116 26.67843521528166 29.38189837373012 22.010162708544105 48-49 22.03502982363559 26.791722299662084 29.097599509314943 22.075648367387387 50-51 22.005677253057847 27.01091810807173 28.845747566163244 22.137657072707174 52-53 22.242214036048082 27.05588566295977 28.709772026698115 21.992128274294032 54-55 21.897760508677234 26.961328850762023 28.756396391105977 22.384514249454767 56-57 22.018314586723168 27.326535928953007 28.699661081075128 21.955488403248697 58-59 22.143730787106875 26.814797902787856 28.791086032991135 22.25038527711413 60-61 22.11270063516631 26.801262014869202 29.14703092980226 21.93900642016223 62-63 21.958383284809045 27.02807069051289 29.07730449604058 21.93624152863749 64-65 21.840657884544036 26.885689706487923 29.07265840641576 22.200994002552278 66-67 21.83934337997847 27.278190951495578 28.704048015135207 22.178417653390742 68-69 21.757796810937656 27.079424818905213 28.90580624641939 22.25697212373774 70-71 21.843197119148268 27.13026296905172 28.753718490684204 22.272821421115808 72-73 21.97366682812982 27.18411528795077 28.673123561713986 22.169094322205424 74-75 21.796303613084262 27.46577680027303 28.476499315189418 22.261420271453286 76-77 21.944090901120198 27.265075388667704 28.45410921559328 22.336724494618824 78-79 22.065288850389642 27.299147306928724 28.377778756572198 22.25778508610944 80-81 21.850732854292428 27.675602229942083 28.320884277815452 22.15278063795004 82-83 21.92906156373828 27.49303799507307 28.333372073439822 22.24452836774882 84-85 21.934335588202913 27.37199639547069 28.223953809832537 22.46971420649386 86-87 22.044514396739608 27.20923642131488 28.321464322630497 22.424784859315018 88-89 22.027991704107738 27.223200443013045 28.253295480402464 22.495512372476757 90-91 21.86461689026628 27.384373853117484 28.38306895567427 22.367940300941974 92-93 22.11996299094795 27.14240452003019 28.27657130460986 22.461061184411996 94-95 21.897777775965466 27.13089375430962 28.251282538514893 22.72004593121002 96-97 22.03448431921215 27.08281773476944 28.181506459744988 22.701191486273427 98-99 22.06196202196153 27.21056730561434 27.866355143997684 22.861115528426442 100-101 22.09350587928307 26.60045338160706 28.57351957858343 22.732521160526446 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1984.0 1 1237.0 2 680.5 3 789.5 4 551.5 5 465.0 6 878.5 7 2016.0 8 3359.0 9 3784.5 10 2967.0 11 2147.0 12 2112.5 13 2245.5 14 2405.5 15 2513.5 16 2484.5 17 2602.5 18 2691.5 19 2807.5 20 3036.5 21 3353.0 22 3916.5 23 4649.0 24 5467.5 25 6514.5 26 8066.0 27 9546.5 28 10516.5 29 11913.5 30 14012.0 31 16269.5 32 18525.0 33 20613.0 34 23015.0 35 25072.5 36 27181.5 37 29837.0 38 31516.5 39 32366.0 40 33812.5 41 37007.0 42 39937.0 43 42183.5 44 44555.5 45 46375.0 46 45954.0 47 45623.5 48 48264.5 49 48143.0 50 45246.0 51 43190.0 52 41431.0 53 40274.5 54 39320.0 55 36433.0 56 32330.5 57 28962.0 58 26076.0 59 23051.5 60 19744.5 61 16362.5 62 13312.5 63 10562.0 64 8272.0 65 6599.0 66 5000.5 67 3893.5 68 2924.0 69 1964.5 70 1483.0 71 1174.0 72 958.5 73 816.5 74 651.5 75 539.0 76 387.0 77 220.5 78 143.0 79 108.5 80 81.0 81 50.5 82 38.0 83 29.5 84 12.5 85 12.0 86 10.0 87 6.5 88 5.5 89 3.5 90 2.0 91 1.0 92 1.0 93 2.5 94 4.0 95 2.0 96 1.5 97 2.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3046383487836441 2 0.28779086329120107 3 0.27558254047059016 4 0.26150227481748556 5 0.2613394971798774 6 0.21942425549578 7 0.20990176369570349 8 0.22333091879837547 9 0.2218659200599022 10-11 0.2260574442283119 12-13 0.22239494738212862 14-15 0.2203195325026248 16-17 0.21804064557611075 18-19 0.2176743958914924 20-21 0.20493704574865504 22-23 0.19639121977422744 24-25 0.1974899688280824 26-27 0.21116329038716664 28-29 0.22345300202658158 30-31 0.2174302294350802 32-33 0.22117411510006757 34-35 0.21478509282394787 36-37 0.20225121472812066 38-39 0.20334996378197565 40-41 0.19683885827764983 42-43 0.21059356865553808 44-45 0.20603579480251002 46-47 0.20514051779566525 48-49 0.21397120463590713 50-51 0.22178453124109812 52-53 0.23525438075317212 54-55 0.21063426306494015 56-57 0.18479331309464705 58-59 0.18556650687328577 60-61 0.1934612222972808 62-63 0.20184427063410026 64-65 0.18841511553142828 66-67 0.19451927694173374 68-69 0.19919913402296793 70-71 0.192565945290436 72-73 0.1940309440289093 74-75 0.19683885827764983 76-77 0.2108377351119503 78-79 0.21747092384448224 80-81 0.2066869053529426 82-83 0.20758218235978743 84-85 0.198181773787917 86-87 0.1983852458349272 88-89 0.20518121220506727 90-91 0.2050998233862632 92-93 0.20212913149991454 94-95 0.2025360755939349 96-97 0.19081608568614844 98-99 0.198181773787917 100-101 0.18963594781348939 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1228670.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.18521288267839 #Duplication Level Percentage of deduplicated Percentage of total 1 79.64370029037433 45.54441955869291 2 11.67307195168547 13.350542091039113 3 3.546725769163829 6.084608044383446 4 1.6087699361247285 3.6799140510618207 5 0.8552815475757974 2.4454728686374305 6 0.5435005811776882 1.864811786190332 7 0.37456749455596083 1.499380534057987 8 0.25982756625648523 1.1886635751332264 9 0.19811253087800607 1.0196196527686479 >10 1.1572095432243468 12.313235057416607 >50 0.09119396145794474 3.6260533356163456 >100 0.04533041203827674 4.5711238415115325 >500 0.0018531263859824926 0.7563914384831012 >1k 5.701927341484593E-4 0.7726947349263192 >5k 2.8509636707422966E-4 1.2830694300812602 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8830 0.718663270039962 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6910 0.5623967379361423 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4742 0.38594577876891273 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1757 0.14300015463875573 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1694 0.13787265905409915 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1286 0.10466602098203749 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 8.13888188040727E-5 0.0 0.0 0.0 0.0 16-17 8.13888188040727E-5 0.0 0.0 4.069440940203635E-5 0.0 18-19 8.13888188040727E-5 0.0 0.0 8.13888188040727E-5 0.0 20-21 8.13888188040727E-5 0.0 0.0 8.13888188040727E-5 0.0 22-23 8.13888188040727E-5 0.0 0.0 1.2208322820610906E-4 0.0 24-25 1.627776376081454E-4 0.0 0.0 1.627776376081454E-4 0.0 26-27 1.627776376081454E-4 0.0 0.0 4.883329128244362E-4 0.0 28-29 2.0347204701018175E-4 0.0 0.0 0.0010580546444529451 0.0 30-31 2.441664564122181E-4 0.0 0.0 0.003133469523956799 0.0 32-33 2.441664564122181E-4 0.0 0.0 0.006551799913727853 0.0 34-35 2.441664564122181E-4 0.0 0.0 0.010336379988117234 0.0 36-37 2.441664564122181E-4 0.0 0.0 0.015789430847990102 0.0 38-39 3.255552752162908E-4 0.0 0.0 0.023684146271985153 0.0 40-41 3.255552752162908E-4 0.0 0.0 0.03206719460880464 0.0 42-43 3.662496846183271E-4 0.0 0.0 0.04293260191914835 0.0 44-45 4.883329128244362E-4 0.0 0.0 0.054408425370522595 0.0 46-47 5.290273222264726E-4 0.0 0.0 0.07137799409117176 0.0 48-49 5.697217316285089E-4 0.0 0.0 0.08936492304687182 0.0 50-51 5.697217316285089E-4 0.0 0.0 0.11365948545988752 0.0 52-53 5.697217316285089E-4 0.0 0.0 0.14206418322250888 0.0 54-55 5.697217316285089E-4 0.0 0.0 0.17343957287147893 0.0 56-57 6.511105504325816E-4 0.0 0.0 0.21388981581710304 0.0 58-59 6.511105504325816E-4 0.0 0.0 0.26304866237476293 0.0 60-61 6.511105504325816E-4 0.0 0.0 0.31757917097349164 0.0 62-63 6.511105504325816E-4 0.0 0.0 0.3707260696525511 0.0 64-65 6.511105504325816E-4 0.0 0.0 0.4302619906077303 0.0 66-67 7.324993692366542E-4 4.069440940203635E-5 0.0 0.5062384529613322 0.0 68-69 7.324993692366542E-4 8.13888188040727E-5 0.0 0.5959696256928224 0.0 70-71 7.731937786386906E-4 8.13888188040727E-5 0.0 0.6928223200696688 0.0 72-73 8.545825974427633E-4 8.13888188040727E-5 0.0 0.7895936256277112 0.0 74-75 8.952770068447997E-4 8.13888188040727E-5 0.0 0.9037821384098252 0.0 76-77 8.952770068447997E-4 8.13888188040727E-5 0.0 1.0238713405552344 0.0 78-79 9.359714162468361E-4 8.13888188040727E-5 0.0 1.1569827537092956 0.0 80-81 0.0011394434632570178 8.13888188040727E-5 0.0 1.2988841592941962 0.0 82-83 0.0012208322820610904 8.13888188040727E-5 0.0 1.4716319272058405 0.0 84-85 0.0013022211008651631 8.13888188040727E-5 0.0 1.648042191963668 0.0 86-87 0.0013836099196692358 8.13888188040727E-5 0.0 1.8476075756712542 0.0 88-89 0.0013836099196692358 8.13888188040727E-5 0.0 2.0749265465910294 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 45 1.5100188E-5 52.813206 2 GGTATCA 4035 0.0 46.43175 1 GTATCAA 7135 0.0 41.58656 1 TATCAAC 9090 0.0 32.3677 2 ATCAACG 9230 0.0 31.92303 3 TCAACGC 9275 0.0 31.663088 4 CAACGCA 9445 0.0 31.143494 5 AACGCAG 9625 0.0 30.6017 6 GTACATG 6935 0.0 27.975386 1 ACGCAGA 11055 0.0 26.515434 7 CGCAGAG 11065 0.0 26.49147 8 TACATGG 7230 0.0 26.297033 2 GCAGAGT 11690 0.0 24.665695 9 ACATGGG 7410 0.0 24.435482 3 GAGTACT 6715 0.0 23.906664 12-13 CATGGGG 2990 0.0 22.727074 4 GTACTTT 7310 0.0 21.829937 14-15 AGAGTAC 10505 0.0 21.543444 10-11 AGTACTT 6985 0.0 21.350672 12-13 TTACGCG 80 4.9993992E-5 20.777388 82-83 >>END_MODULE