FastQCFastQC Report
Thu 26 May 2016
SRR937565_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937565_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228670
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86240.701897173366323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74160.6035794802510032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52570.42786102045301017No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19120.155615421553387No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13900.11313045813766105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51450.045.5310971
GTATCAA83050.036.731781
TATCAAC105000.028.7745342
ATCAACG104700.028.7662373
TCAACGC105600.028.4760864
CAACGCA106750.028.2138185
AACGCAG109400.027.569336
ACGCAGA122300.024.585697
CGCAGAG123050.024.4744428
GAGTACT72250.024.32590512-13
GTACATG67900.023.4434551
GCAGAGT129450.022.970879
GTACTTT77200.022.64124514-15
TACATGG70550.022.0186772
TAAGGTG5350.021.310715
AGTACTT74400.021.22872412-13
GGACCGA1805.171685E-621.1099536
AGAGTAC113550.020.979210-11
ACATGGG71750.020.7234923
CAGAGTA127850.020.45322210-11
Produced by FastQC (version 0.11.5)