##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937565_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1228670 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.972028290753418 31.0 31.0 34.0 30.0 34.0 2 32.19576533975762 33.0 31.0 34.0 30.0 34.0 3 32.24189977780853 34.0 31.0 34.0 30.0 34.0 4 35.930746254079615 37.0 35.0 37.0 35.0 37.0 5 35.836285577087416 37.0 35.0 37.0 35.0 37.0 6 35.892183417842055 37.0 35.0 37.0 35.0 37.0 7 35.84023212091123 37.0 35.0 37.0 35.0 37.0 8 35.8041036242441 37.0 35.0 37.0 35.0 37.0 9 37.43749582882304 39.0 37.0 39.0 34.0 39.0 10-11 37.341247039481715 39.0 37.0 39.0 34.0 39.0 12-13 37.21384464502267 39.0 37.0 39.0 33.0 39.0 14-15 38.3126034655359 40.0 38.0 41.0 33.0 41.0 16-17 38.26287326947024 40.0 38.0 41.0 33.0 41.0 18-19 38.25944313770174 40.0 38.0 41.0 33.5 41.0 20-21 38.15581930054449 40.0 37.5 41.0 33.0 41.0 22-23 38.09136586715717 40.0 37.0 41.0 33.0 41.0 24-25 38.093531216681455 40.0 37.0 41.0 33.0 41.0 26-27 37.87477150089121 40.0 37.0 41.0 33.0 41.0 28-29 37.823204359185134 40.0 37.0 41.0 33.0 41.0 30-31 37.7935621444326 40.0 37.0 41.0 33.0 41.0 32-33 37.626608853475716 40.0 36.5 41.0 33.0 41.0 34-35 37.43819943516159 39.5 36.0 41.0 32.5 41.0 36-37 37.218956269787654 39.0 36.0 41.0 32.0 41.0 38-39 37.04584510080005 39.0 36.0 41.0 31.0 41.0 40-41 36.8207224071557 39.0 35.0 41.0 31.0 41.0 42-43 36.544286097975856 39.0 35.0 40.0 30.5 41.0 44-45 36.15076831044951 39.0 35.0 40.0 30.0 41.0 46-47 35.71870274361709 38.5 35.0 40.0 28.5 41.0 48-49 35.286108963350614 38.0 35.0 40.0 27.0 41.0 50-51 34.98398390129164 38.0 34.0 40.0 26.0 41.0 52-53 34.6720291046416 38.0 34.0 40.0 25.0 41.0 54-55 34.396062408946264 38.0 34.0 40.0 24.0 41.0 56-57 34.07494770768392 38.0 33.0 40.0 23.5 41.0 58-59 33.7782016326597 37.0 33.0 40.0 23.0 41.0 60-61 33.38336005599551 37.0 32.5 39.5 22.0 41.0 62-63 32.9243503137539 36.0 32.0 39.0 20.5 40.0 64-65 32.49273767569811 36.0 31.0 39.0 19.5 40.0 66-67 32.02515036584274 35.0 31.0 38.5 18.0 40.0 68-69 31.503982354904082 35.0 30.5 38.0 15.5 40.0 70-71 31.04149527537907 34.5 30.0 37.5 12.5 39.5 72-73 30.522235832241364 34.0 29.5 37.0 8.0 39.0 74-75 30.01160685944965 34.0 29.0 36.0 7.0 39.0 76-77 28.86886836986335 32.5 28.0 35.0 7.0 37.0 78-79 29.48160083667706 34.0 29.0 35.0 4.0 37.0 80-81 29.290450242945624 34.0 29.0 35.0 2.0 37.0 82-83 28.853401645681913 34.0 29.0 35.0 2.0 36.5 84-85 28.37618848022659 33.0 27.5 35.0 2.0 36.0 86-87 27.941064728527596 33.0 27.0 35.0 2.0 36.0 88-89 27.444224242473567 33.0 26.0 35.0 2.0 35.0 90-91 26.800577453669415 32.0 25.0 34.5 2.0 35.0 92-93 26.20280669341646 32.0 24.5 34.0 2.0 35.0 94-95 25.54973263773023 32.0 22.5 34.0 2.0 35.0 96-97 24.829895740923114 31.0 19.0 34.0 2.0 35.0 98-99 23.753202243075847 31.0 4.5 34.0 2.0 35.0 100-101 21.20944313770174 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20285962441357341 1101 2 0.22108605719643037 1101 3 0.16013615649104196 1101 4 0.1010092707137531 1101 5 0.0739173256097061 1101 6 0.11082365499225233 1101 7 0.1278158785363317 1101 8 0.16018762513017037 1101 9 0.24461265245906816 1101 10-11 0.22959799650575263 1101 12-13 0.309057379627518 1101 14-15 0.33259862426088915 1101 16-17 0.2758292097232058 1101 18-19 0.2194629848062135 1101 20-21 0.19742718333459663 1101 22-23 0.3904117462395007 1101 24-25 0.2145247577371947 1101 26-27 0.07762598628361417 1101 28-29 0.18497891670702415 1101 30-31 0.3165403592783065 1101 32-33 0.23902964730678633 1101 34-35 0.2353316509099983 1101 36-37 0.12051836294378404 1101 38-39 0.24295784235838624 1101 40-41 0.28056912578477977 1101 42-43 0.14809985569287676 1101 44-45 0.27263909302201483 1101 46-47 0.14740746240974545 1101 48-49 0.0915300657121847 1101 50-51 0.16960771316313128 1101 52-53 0.16702474604871753 1101 54-55 0.216074702333664 1101 56-57 0.2628066686228152 1101 58-59 0.0788141889606564 1101 60-61 0.2663233394476592 1101 62-63 0.2036110434693228 1101 64-65 0.1873069133529981 1101 66-67 0.12375247565973879 1101 68-69 0.29724191908426434 1101 70-71 0.08003838087760329 1101 72-73 0.14760095373026516 1101 74-75 0.3925538787201148 1101 76-77 0.22926447849085463 1101 78-79 0.42303462137837045 1101 80-81 0.49396244122882393 1101 82-83 0.5545339851610791 1101 84-85 0.6371479935745974 1101 86-87 0.5254783685836948 1101 88-89 0.7097091009820495 1101 90-91 0.7707926171336723 1101 92-93 0.9900374733233903 1101 94-95 1.0627331879401112 1101 96-97 1.0791006741973597 1101 98-99 1.0695907901790882 1101 100-101 1.1562253042948107 1102 1 0.16908652816980307 1102 2 0.1378778737822728 1102 3 0.21981615510048869 1102 4 0.28377856031413984 1102 5 0.11455020179819542 1102 6 0.2080225564540754 1102 7 0.0994537781035234 1102 8 0.062258682473171234 1102 9 0.1334352003028414 1102 10-11 0.18199235780661382 1102 12-13 0.13355156537085122 1102 14-15 0.25474277263306533 1102 16-17 0.2363917759918266 1102 18-19 0.16578018770551495 1102 20-21 0.1260534891523264 1102 22-23 0.14615256423328304 1102 24-25 0.2037375299817228 1102 26-27 0.22227843452890994 1102 28-29 0.20484907366588345 1102 30-31 0.4147061307754143 1102 32-33 0.34093818357418826 1102 34-35 0.21257956942349665 1102 36-37 0.26086443723416863 1102 38-39 0.36168296446646053 1102 40-41 0.39720189768166847 1102 42-43 0.2986421720092949 1102 44-45 0.3838530472379986 1102 46-47 0.32194466843780134 1102 48-49 0.4692025379445468 1102 50-51 0.4129799820268758 1102 52-53 0.2837513803849916 1102 54-55 0.42019465505480724 1102 56-57 0.32807235147205915 1102 58-59 0.4716271941230872 1102 60-61 0.44399320439004697 1102 62-63 0.33380824150169275 1102 64-65 0.47176963779720893 1102 66-67 0.2643575407550749 1102 68-69 0.0026768150533946766 1102 70-71 0.0123014216730013 1102 72-73 0.22519520726314113 1102 74-75 0.22261158673840242 1102 76-77 0.1857961744460752 1102 78-79 0.2187856078030812 1102 80-81 0.23770407617346478 1102 82-83 0.36391284177750904 1102 84-85 0.24851534335124015 1102 86-87 0.32834856030641646 1102 88-89 0.37279161301903585 1102 90-91 0.31343832118887605 1102 92-93 0.6986576085548322 1102 94-95 0.7951661371327212 1102 96-97 0.92935140858404 1102 98-99 1.0660922415705123 1102 100-101 1.513779057923287 1103 1 0.12452481152869055 1103 2 0.17943405472409069 1103 3 0.19358321393441003 1103 4 0.05495669673180004 1103 5 0.12933003294390488 1103 6 0.173925652999678 1103 7 0.0861371740982051 1103 8 0.08309729894595819 1103 9 0.14273764917604126 1103 10-11 0.1122524870760131 1103 12-13 0.24266337942790983 1103 14-15 0.2329103368811971 1103 16-17 0.2430805618932439 1103 18-19 0.29858594341904166 1103 20-21 0.3365505534203379 1103 22-23 0.3704086908342319 1103 24-25 0.043495602692459556 1103 26-27 0.12270984796567319 1103 28-29 0.05763680411753569 1103 30-31 0.21634298360002902 1103 32-33 0.20288875547863938 1103 34-35 0.2863232130888065 1103 36-37 0.14854010622320146 1103 38-39 0.25653229006542944 1103 40-41 0.4190608411715857 1103 42-43 0.42344877597044217 1103 44-45 0.3642395300587751 1103 46-47 0.5034676705274919 1103 48-49 0.5095771364627595 1103 50-51 0.5563799970479266 1103 52-53 0.4690322961389839 1103 54-55 0.5791752558085008 1103 56-57 0.6051910928846524 1103 58-59 0.4886917713909398 1103 60-61 0.3552415421339745 1103 62-63 0.14387808939145685 1103 64-65 0.2104875026872719 1103 66-67 0.2850905680713005 1103 68-69 0.3576949507641771 1103 70-71 0.2919620342668239 1103 72-73 0.21062787981747277 1103 74-75 0.4871015999480903 1103 76-77 0.35077746440097 1103 78-79 0.4328316164004242 1103 80-81 0.5206110030872111 1103 82-83 0.5647575550300523 1103 84-85 0.7318119340143348 1103 86-87 0.8595748966277625 1103 88-89 0.9476231784335063 1103 90-91 0.7969469737563735 1103 92-93 1.0518124711784793 1103 94-95 0.9024522307315479 1103 96-97 1.1231998461737795 1103 98-99 1.4984924288918613 1103 100-101 2.093953037044024 1104 1 0.10624838318750207 1104 2 0.03638669340560341 1104 3 0.14458329743556675 1104 4 0.07069307369732769 1104 5 0.12138485280137701 1104 6 0.015518567791680482 1104 7 0.08391002760215116 1104 8 0.123600299151569 1104 9 0.05333394621390397 1104 10-11 0.08435614164339 1104 12-13 0.07989358314380013 1104 14-15 0.33085046206110746 1104 16-17 0.2642968146305975 1104 18-19 0.3850888789785927 1104 20-21 0.06079852672151276 1104 22-23 0.13754730365552348 1104 24-25 0.03859843930761997 1104 26-27 0.024182296623685318 1104 28-29 0.11004859591771776 1104 30-31 0.14862803321139495 1104 32-33 0.26486659265497536 1104 34-35 0.10643747499692324 1104 36-37 0.14080569916672658 1104 38-39 0.11636908570397253 1104 40-41 0.20766912825754957 1104 42-43 0.29375060123393837 1104 44-45 0.16186504442142535 1104 46-47 0.055211004127194485 1104 48-49 0.2561544868183603 1104 50-51 0.41233511113475174 1104 52-53 0.3370773454630296 1104 54-55 0.3203528645687683 1104 56-57 0.17733968544119705 1104 58-59 0.24083160081512744 1104 60-61 0.15527242858406254 1104 62-63 0.3980791853217198 1104 64-65 0.5252453448377565 1104 66-67 0.4168652729665361 1104 68-69 0.6024646806065057 1104 70-71 0.5903370388683697 1104 72-73 0.5335535718669924 1104 74-75 0.6709960670535722 1104 76-77 0.6498465162677576 1104 78-79 0.6235341215249726 1104 80-81 0.6109060584718229 1104 82-83 0.530748116705535 1104 84-85 0.5043013549562438 1104 86-87 0.8509778687302969 1104 88-89 0.7566555795766696 1104 90-91 0.7580551867687291 1104 92-93 0.8201001227936864 1104 94-95 0.6129640389626019 1104 96-97 0.9681310115895734 1104 98-99 0.9614506863932704 1104 100-101 1.363273678799814 1105 1 0.07099599730406325 1105 2 0.07893146016886021 1105 3 0.062295595680637916 1105 4 -0.020669308771985584 1105 5 0.041825621663896584 1105 6 0.11464923811512051 1105 7 -0.06190608231919015 1105 8 0.03035027775456456 1105 9 -0.03449534426146528 1105 10-11 0.1964663282158554 1105 12-13 0.214653790925162 1105 14-15 -0.006290127066584716 1105 16-17 0.06769809167899865 1105 18-19 0.19636848561232512 1105 20-21 0.18839214919184855 1105 22-23 0.17201717056146038 1105 24-25 0.11854540295529148 1105 26-27 0.006598897688554928 1105 28-29 0.08693889183592773 1105 30-31 0.2028795472257201 1105 32-33 0.2069599531883668 1105 34-35 0.14827590551968228 1105 36-37 0.22328362263223056 1105 38-39 0.18513458677475825 1105 40-41 0.2212320067611273 1105 42-43 0.24913596831716234 1105 44-45 0.07122028452400997 1105 46-47 0.14524912371151544 1105 48-49 0.3123319662607784 1105 50-51 0.21194911860065702 1105 52-53 0.24141933823413098 1105 54-55 0.4175354670651146 1105 56-57 0.4804247100783243 1105 58-59 0.3747975330237736 1105 60-61 0.4703853906535258 1105 62-63 0.3781689210616719 1105 64-65 0.41562379434035535 1105 66-67 0.22139494802364723 1105 68-69 0.41053544214230797 1105 70-71 0.29917578822998436 1105 72-73 0.38944287364750707 1105 74-75 0.3537450505783504 1105 76-77 0.36791607392821746 1105 78-79 0.5662566950342587 1105 80-81 0.49973451846130956 1105 82-83 0.3329892529381411 1105 84-85 0.5108535406013459 1105 86-87 0.6003042158244867 1105 88-89 0.8163866074887061 1105 90-91 0.5884893437220953 1105 92-93 0.7761273999756391 1105 94-95 0.8495735121448718 1105 96-97 1.1882756083054424 1105 98-99 1.3248917195440484 1105 100-101 1.328770658295369 1106 1 -0.18069132412292532 1106 2 -0.15048941042707753 1106 3 -0.099864175745914 1106 4 -0.058015946070860025 1106 5 -0.1284598297775119 1106 6 -0.02382699785335518 1106 7 -0.09329777972473607 1106 8 0.05205944811432062 1106 9 -0.24746847980446063 1106 10-11 -0.4303990133616864 1106 12-13 -0.5355279886571438 1106 14-15 -0.32030263491716227 1106 16-17 -0.14883190858637363 1106 18-19 -0.2250027781759485 1106 20-21 -0.051667470972859064 1106 22-23 -0.15315863815865072 1106 24-25 -0.06702280198238952 1106 26-27 0.03330059341341496 1106 28-29 -0.2036386453382164 1106 30-31 -0.14828322290050266 1106 32-33 -0.23851944070278108 1106 34-35 -0.4462574384285176 1106 36-37 -0.2132518619806305 1106 38-39 -0.2382234261222962 1106 40-41 -0.37309877015861304 1106 42-43 -0.515576678411243 1106 44-45 -0.1451239555842534 1106 46-47 -0.10862928299707875 1106 48-49 -0.06942461716764825 1106 50-51 -0.17383226823159248 1106 52-53 -0.30261378014816387 1106 54-55 -0.43796612958766445 1106 56-57 -0.4679136210878099 1106 58-59 -0.35457645524679293 1106 60-61 -0.24546525818016818 1106 62-63 -0.49547613550672054 1106 64-65 -0.8875801260835345 1106 66-67 -0.7084761698102113 1106 68-69 -0.8464248140297919 1106 70-71 -0.5917140029225223 1106 72-73 -0.6478648424755882 1106 74-75 -0.6991435198645561 1106 76-77 -0.7979199794645595 1106 78-79 -0.6828742128021652 1106 80-81 -0.617556213876 1106 82-83 -0.9312138802165109 1106 84-85 -0.7612647443901182 1106 86-87 -0.3054304655968423 1106 88-89 -0.44726455262770415 1106 90-91 -0.5924437030098559 1106 92-93 -0.6333162925824531 1106 94-95 -0.2975379174122246 1106 96-97 -0.28652027666479185 1106 98-99 -0.4627101168277896 1106 100-101 0.17428985793446472 1107 1 0.06344872592122996 1107 2 -0.007390107120237133 1107 3 -0.11450638455760753 1107 4 0.028193699695016505 1107 5 0.04166209152559475 1107 6 0.03929180795569209 1107 7 0.06405122920470774 1107 8 -0.11686595174830217 1107 9 0.009462267914109646 1107 10-11 0.005402183773036029 1107 12-13 0.07530325950299499 1107 14-15 0.08201589368650986 1107 16-17 7.317382588851729E-4 1107 18-19 0.16770678172984788 1107 20-21 0.13650561066781108 1107 22-23 0.013385064618312015 1107 24-25 -0.2268025708961403 1107 26-27 -0.07489015087075757 1107 28-29 0.13465617361968185 1107 30-31 0.17389535378480758 1107 32-33 0.23454042829927602 1107 34-35 0.17674386173829504 1107 36-37 0.07870672893292152 1107 38-39 0.19726070202999324 1107 40-41 0.22970180142415586 1107 42-43 0.22421057259454358 1107 44-45 0.2087943301580637 1107 46-47 0.09895308134400693 1107 48-49 0.005267819786531902 1107 50-51 0.050049710965737404 1107 52-53 0.06144274031107244 1107 54-55 0.11587365551684314 1107 56-57 0.14712845034776478 1107 58-59 0.19498167202235805 1107 60-61 0.28888674358609734 1107 62-63 0.26873094672269815 1107 64-65 0.3499278466374989 1107 66-67 0.31044371047631714 1107 68-69 0.22542872095205624 1107 70-71 0.06795077482254541 1107 72-73 0.3275616980994265 1107 74-75 0.38685914179769654 1107 76-77 0.07979933169234243 1107 78-79 0.1301271486932727 1107 80-81 0.3208094396418133 1107 82-83 0.43991536372422146 1107 84-85 0.5353713806221485 1107 86-87 0.6468150819451068 1107 88-89 0.6941193890855715 1107 90-91 0.8250628591528368 1107 92-93 0.8194824391416162 1107 94-95 1.044051475189093 1107 96-97 1.0597399038866762 1107 98-99 1.2104828352868218 1107 100-101 1.858369867256414 1108 1 0.17340235222320288 1108 2 0.1951181268757196 1108 3 0.11168869427140748 1108 4 0.11957538093487585 1108 5 0.04687103439754736 1108 6 0.14448162873043913 1108 7 0.08047272202399824 1108 8 0.13769252634174478 1108 9 0.10274309733246412 1108 10-11 0.18958873184786995 1108 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2316 60-61 0.3517790568192751 2316 62-63 0.3135681229494338 2316 64-65 0.14882734829862443 2316 66-67 0.2505272814638779 2316 68-69 0.38962099669956274 2316 70-71 0.445687779791232 2316 72-73 0.60246348197618 2316 74-75 0.4366457123736538 2316 76-77 0.26213218971721375 2316 78-79 0.2454171664805891 2316 80-81 0.2748504496967925 2316 82-83 0.1785451707097856 2316 84-85 0.3393534977538337 2316 86-87 0.7209645799311701 2316 88-89 0.7177564910759848 2316 90-91 0.8765849793715788 2316 92-93 0.9736950747515287 2316 94-95 0.9274398120014844 2316 96-97 1.1209573060564324 2316 98-99 1.180183299080074 2316 100-101 1.6581867571220563 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 17.0 5 46.0 6 97.0 7 222.0 8 496.0 9 864.0 10 1453.0 11 2380.0 12 3500.0 13 5034.0 14 6617.0 15 8990.0 16 12002.0 17 16566.0 18 14611.0 19 9788.0 20 8352.0 21 8465.0 22 9109.0 23 10004.0 24 11533.0 25 13777.0 26 16475.0 27 20246.0 28 24952.0 29 30720.0 30 37457.0 31 46570.0 32 58230.0 33 75647.0 34 100025.0 35 137971.0 36 188119.0 37 224788.0 38 118166.0 39 5372.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.899549922164546 20.045398589191617 15.749223273778895 21.305828214864945 2 16.12670611311418 21.26006169272465 39.30493948741322 23.308292706747945 3 19.751845491466383 25.303376822092183 30.344111925903622 24.60066576053782 4 13.467245069872302 17.668780062994944 36.44005306550986 32.42392180162289 5 13.522513239011985 37.939109498390806 33.50117128424521 15.037205978351997 6 31.526365907851577 36.64808288637307 16.845532160791752 14.9800190449836 7 28.35968974582272 33.80525283436561 19.868150113537403 17.966907306274262 8 27.009693408319563 34.926709368666934 18.94739840640693 19.116198816606573 9 26.780828049842516 16.876622689574905 20.24961950727209 36.092929753310486 10-11 25.360552467302043 27.26720763101565 26.1490880382853 21.223151863397007 12-13 26.31540392746085 24.686931647302572 27.459912884974063 21.53775154026252 14-15 23.021681981329404 25.635809452497416 26.553753245379152 24.788755320794028 16-17 21.586430856128985 29.379206784571938 26.94319874335664 22.091163615942442 18-19 21.57650142023489 26.810087330202574 30.645128472250484 20.96828277731205 20-21 22.44778284107899 25.73602123276689 30.898078975019267 20.91811695113485 22-23 22.0990432627548 25.853555310481784 30.85646499627642 21.190936430486996 24-25 21.98145148819455 26.087069758356595 30.921321428862104 21.010157324586746 26-27 21.570631070134187 26.452538906238427 30.80362411376957 21.17320590985782 28-29 21.324589875733732 26.453869769470934 31.1700546615315 21.05148569326384 30-31 21.41641832893223 26.03714831368403 31.167372511484988 21.379060845898753 32-33 21.410590313102787 26.22844213661927 30.909397966907303 21.451569583370635 34-35 22.038098106082185 26.185346757062515 30.56496048572847 21.211594651126827 36-37 22.092813631689314 26.01332420148185 30.575017712292148 21.318844454536688 38-39 21.68568452066055 26.292332359380467 30.686596075431154 21.335387044527824 40-41 21.742276293402167 26.181879001537027 30.69001117876791 21.385833526292895 42-43 21.888119231811864 26.239196285891285 30.26000551938828 21.61267896290857 44-45 21.822702597117207 26.607388476971032 29.945957824314096 21.62395110159766 46-47 22.09918041459464 26.967004972856827 29.348889449567416 21.584925162981108 48-49 22.315878144660488 26.91931112503764 29.06012192045057 21.7046888098513 50-51 22.227856137123883 27.12587594716238 28.807572415701532 21.83869550001221 52-53 22.4800393921883 27.296059967281693 28.52181627288043 21.702084367649572 54-55 22.13507288368724 27.28743275248846 28.559865545671336 22.01762881815296 56-57 22.207915876516886 27.568834593503546 28.53337348515061 21.689876044828964 58-59 22.351363669659065 27.075659045960265 28.64003353219335 21.932943752187324 60-61 22.273352486835357 27.097023610896336 28.95122368089072 21.678400221377586 62-63 22.09511097365444 27.223623918546068 28.998754751072298 21.682510356727192 64-65 22.048719346936117 27.15281564618653 28.824053651509356 21.974411355367998 66-67 22.079036681940632 27.50718256325946 28.50838711777776 21.905393637022147 68-69 22.032726444041117 27.27200957132509 28.695296540161312 21.999967444472478 70-71 22.110045821904986 27.483905361081497 28.45682730106538 21.94922151594814 72-73 22.267044853378042 27.451349833559867 28.379223062335697 21.902382250726397 74-75 22.134340384318 27.78134079940098 28.007683104495108 22.076635711785915 76-77 22.17340701734396 27.561957238314598 28.272481626474153 21.992154117867287 78-79 22.263463745350663 27.547917667070898 28.16854810486135 22.020070482717085 80-81 22.043876712217276 27.994131866164228 28.096193444944532 21.865797976673964 82-83 22.1239676316009 27.855217135636778 28.074519754123877 21.946295478638444 84-85 22.06354528932575 27.722401594573633 28.003600650135475 22.210452465965147 86-87 22.21479323170583 27.42546818918017 28.107465796348897 22.252272782765104 88-89 22.12778858440427 27.567206817127467 28.088420812748744 22.216583785719518 90-91 22.1440664859629 27.770424816650795 28.070580287009676 22.01492841037663 92-93 22.168897592293256 27.51965998121497 28.08109467556758 22.230347750924196 94-95 22.06617724856959 27.431775822637487 27.975697298705104 22.526349630087818 96-97 22.191109085433844 27.477109394711352 27.886169598020622 22.44561192183418 98-99 22.062084482785984 27.567492997478155 27.648190372554915 22.722232147180943 100-101 22.014217372936198 27.370070907784065 28.10308043080284 22.512631288476896 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 825.0 1 579.5 2 527.0 3 699.5 4 522.5 5 428.5 6 786.0 7 1776.5 8 2809.0 9 3185.5 10 2808.0 11 2241.5 12 2152.0 13 2276.5 14 2457.5 15 2644.5 16 2748.0 17 2852.5 18 3014.5 19 3161.5 20 3346.5 21 3671.5 22 4170.0 23 4854.0 24 5722.0 25 6882.5 26 8328.5 27 9931.5 28 11036.0 29 12308.0 30 14332.0 31 16553.0 32 18785.0 33 20788.5 34 23266.0 35 25312.0 36 27495.0 37 29920.5 38 31698.5 39 32743.5 40 33992.0 41 37349.0 42 40617.0 43 43042.0 44 44880.0 45 46277.5 46 45929.5 47 45656.5 48 48494.0 49 48530.0 50 45543.0 51 43043.5 52 41033.0 53 40017.0 54 38849.5 55 35962.0 56 32020.0 57 28435.0 58 25743.5 59 22787.5 60 19093.0 61 15858.5 62 13092.5 63 10306.5 64 7943.5 65 6212.5 66 4643.0 67 3674.5 68 2670.5 69 1703.0 70 1263.5 71 1031.5 72 912.5 73 765.0 74 617.0 75 506.5 76 358.5 77 225.5 78 134.5 79 91.0 80 62.5 81 31.0 82 14.0 83 11.0 84 6.0 85 3.5 86 2.5 87 2.5 88 2.0 89 0.0 90 0.0 91 1.0 92 1.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03564830263618384 2 0.0 3 0.0 4 0.0 5 0.008464437155623561 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005249578812862689 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.4416645641221806E-4 22-23 0.0012208322820610904 24-25 0.0 26-27 8.952770068447997E-4 28-29 0.0014649987384733087 30-31 4.069440940203635E-5 32-33 0.0 34-35 0.0 36-37 2.8486086581425445E-4 38-39 0.0 40-41 1.2208322820610903E-4 42-43 0.022219147533511847 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.0347204701018172E-4 84-85 2.4416645641221806E-4 86-87 0.0 88-89 0.0 90-91 0.011394434632570179 92-93 0.0026044422017303262 94-95 0.0 96-97 0.0 98-99 4.4763850342239984E-4 100-101 0.014080265653104577 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1228670.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.20073723381365 #Duplication Level Percentage of deduplicated Percentage of total 1 78.79640468551804 44.28416034700041 2 12.219164545537865 13.73452111681011 3 3.652264413973693 6.157798579144318 4 1.651678595582647 3.713022189802187 5 0.8859560579522644 2.4895691806840294 6 0.553387884303903 1.8660484244483828 7 0.4130043286964132 1.6247803427446306 8 0.2859450677977589 1.2856258894885502 9 0.18544283181296145 0.9379821476353091 >10 1.2088726792695312 12.506083104435724 >50 0.09625628675624122 3.679315547033616 >100 0.048287458848204685 4.704389934412008 >500 0.0026101283091184164 1.0120058430561119 >1k 2.9001425656871295E-4 0.2690967381036626 >5k 4.350213848530694E-4 1.735600615201 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8624 0.701897173366323 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7416 0.6035794802510032 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5257 0.42786102045301017 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1912 0.155615421553387 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1390 0.11313045813766105 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 4.069440940203635E-5 0.0 0.0 0.0 0.0 20-21 8.13888188040727E-5 0.0 0.0 0.0 0.0 22-23 8.13888188040727E-5 0.0 0.0 4.069440940203635E-5 0.0 24-25 1.627776376081454E-4 0.0 0.0 8.13888188040727E-5 0.0 26-27 1.627776376081454E-4 0.0 0.0 4.069440940203635E-4 0.0 28-29 2.8486086581425445E-4 0.0 0.0 8.952770068447997E-4 0.0 30-31 3.255552752162908E-4 0.0 0.0 0.002889303067544581 0.0 32-33 3.255552752162908E-4 0.0 0.0 0.006348327866717671 0.0 34-35 3.662496846183271E-4 0.0 0.0 0.010336379988117234 0.0 36-37 4.883329128244362E-4 0.0 0.0 0.015748736438588068 0.0 38-39 5.697217316285089E-4 0.0 0.0 0.023684146271985153 0.0 40-41 5.697217316285089E-4 0.0 0.0 0.03210788901820668 0.0 42-43 6.104161410305452E-4 0.0 0.0 0.042851213100344274 0.0 44-45 7.324993692366542E-4 0.0 0.0 0.05416425891411038 0.0 46-47 7.324993692366542E-4 0.0 0.0 0.07076757795014121 0.0 48-49 8.13888188040727E-4 0.0 0.0 0.08863242367763516 0.0 50-51 8.13888188040727E-4 0.0 0.0 0.1129676805000529 0.0 52-53 8.13888188040727E-4 0.0 0.0 0.14088404534984983 0.0 54-55 8.13888188040727E-4 0.0 0.0 0.17189318531420153 0.0 56-57 8.13888188040727E-4 0.0 0.0 0.21246551148803178 0.0 58-59 8.13888188040727E-4 0.0 0.0 0.26199060773031 0.0 60-61 8.13888188040727E-4 0.0 0.0 0.31656181073844075 0.0 62-63 8.13888188040727E-4 0.0 0.0 0.36987148705510836 0.0 64-65 8.13888188040727E-4 0.0 0.0 0.428756297459855 0.0 66-67 8.952770068447997E-4 0.0 0.0 0.5039595660348182 0.0 68-69 8.952770068447997E-4 0.0 0.0 0.5924699064842471 0.0 70-71 9.766658256488724E-4 0.0 0.0 0.6876541300756103 0.0 72-73 9.766658256488724E-4 0.0 0.0 0.7841812691772404 0.0 74-75 0.0010173602350509088 0.0 0.0 0.8977593658183238 0.0 76-77 0.0010580546444529451 0.0 0.0 1.018825233789382 0.0 78-79 0.0010580546444529451 0.0 0.0 1.152058730171649 0.0 80-81 0.0013022211008651631 0.0 0.0 1.2948554127633947 0.0 82-83 0.0013022211008651631 0.0 0.0 1.4673590142186268 0.0 84-85 0.0013429155102671995 0.0 0.0 1.6429553907884136 0.0 86-87 0.0014649987384733085 0.0 0.0 1.841747580717361 0.0 88-89 0.0015463875572773813 0.0 0.0 2.0664621094354056 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5145 0.0 45.531097 1 GTATCAA 8305 0.0 36.73178 1 TATCAAC 10500 0.0 28.774534 2 ATCAACG 10470 0.0 28.766237 3 TCAACGC 10560 0.0 28.476086 4 CAACGCA 10675 0.0 28.213818 5 AACGCAG 10940 0.0 27.56933 6 ACGCAGA 12230 0.0 24.58569 7 CGCAGAG 12305 0.0 24.474442 8 GAGTACT 7225 0.0 24.325905 12-13 GTACATG 6790 0.0 23.443455 1 GCAGAGT 12945 0.0 22.97087 9 GTACTTT 7720 0.0 22.641245 14-15 TACATGG 7055 0.0 22.018677 2 TAAGGTG 535 0.0 21.31071 5 AGTACTT 7440 0.0 21.228724 12-13 GGACCGA 180 5.171685E-6 21.109953 6 AGAGTAC 11355 0.0 20.9792 10-11 ACATGGG 7175 0.0 20.723492 3 CAGAGTA 12785 0.0 20.453222 10-11 >>END_MODULE