FastQCFastQC Report
Thu 26 May 2016
SRR937564_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937564_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093275
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78830.7210445679266425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61150.559328622716151No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42620.3898378724474629No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15790.14442843749285403No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15630.142964944776017No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11800.1079325878667307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36950.052.8927731
GTATCAA63950.040.2277451
TATCAAC81150.031.1582372
ATCAACG81950.030.968643
TCAACGC82700.030.6345394
CAACGCA83900.030.1977625
AACGCAG86300.029.398216
GTACATG61150.026.439391
ACGCAGA97900.025.914877
CGCAGAG98800.025.6788028
TACATGG63700.025.0697292
GCAGAGT104100.024.0964879
ACATGGG62750.023.7818573
GAGTACT60850.023.7699112-13
GTACTTT64400.022.31158314-15
CATGGGA36650.022.0477644
ATAACGC1302.9305747E-421.9350053
TGGTATC16850.021.7190382
AGTACTT62550.021.22537412-13
CAGAGTA103450.021.2149610-11
Produced by FastQC (version 0.11.5)