##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937564_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1093275 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.882187006928724 33.0 31.0 34.0 30.0 34.0 2 31.98772038142279 33.0 31.0 34.0 30.0 34.0 3 32.019004367611075 34.0 31.0 34.0 30.0 34.0 4 35.63748645125883 37.0 35.0 37.0 35.0 37.0 5 35.64019848619972 37.0 35.0 37.0 35.0 37.0 6 35.71461434680204 37.0 35.0 37.0 35.0 37.0 7 35.64227115775994 37.0 35.0 37.0 33.0 37.0 8 35.64908280167387 37.0 35.0 37.0 33.0 37.0 9 37.23331366764995 39.0 37.0 39.0 34.0 39.0 10-11 37.157277903546685 39.0 37.0 39.0 34.0 39.0 12-13 37.05063776268551 39.0 37.0 39.0 33.0 39.0 14-15 38.172797786467264 40.0 38.0 41.0 33.5 41.0 16-17 38.08818641238481 40.0 38.0 41.0 33.0 41.0 18-19 38.03714618920217 40.0 38.0 41.0 33.0 41.0 20-21 38.05836546157188 40.0 38.0 41.0 33.5 41.0 22-23 38.06128375751755 40.0 38.0 41.0 33.5 41.0 24-25 37.981108595733005 40.0 38.0 41.0 33.0 41.0 26-27 37.871260204431636 40.0 38.0 41.0 33.0 41.0 28-29 37.7139498296403 40.0 37.5 41.0 33.0 41.0 30-31 37.44304543687544 40.0 37.0 41.0 32.5 41.0 32-33 37.271165992087994 39.5 37.0 41.0 32.0 41.0 34-35 37.22368251354874 40.0 36.5 41.0 32.0 41.0 36-37 37.04382200269831 40.0 36.0 41.0 31.0 41.0 38-39 36.85825798632549 39.5 36.0 41.0 31.0 41.0 40-41 36.59866090416409 39.0 35.5 41.0 30.5 41.0 42-43 36.30222313690517 39.0 35.0 41.0 30.0 41.0 44-45 35.98587775262399 39.0 35.0 41.0 29.5 41.0 46-47 35.5495062998788 39.0 35.0 40.0 27.5 41.0 48-49 35.126863780841965 39.0 35.0 40.0 26.0 41.0 50-51 34.22725892387551 37.5 34.0 39.5 24.0 40.5 52-53 34.33162927900116 38.0 34.0 39.5 24.0 40.5 54-55 34.57632434657337 38.0 34.0 40.0 24.0 41.0 56-57 34.31770734719078 38.0 34.0 40.0 23.0 41.0 58-59 34.01833207564428 38.0 34.0 40.0 22.5 41.0 60-61 33.65534197708719 37.5 33.0 40.0 21.5 41.0 62-63 33.27195902220393 37.0 33.0 40.0 19.5 41.0 64-65 32.82490727401614 36.0 32.5 39.0 18.0 41.0 66-67 32.307908806110085 36.0 31.5 39.0 13.0 40.0 68-69 31.83857355194256 35.0 31.0 38.5 9.5 40.0 70-71 31.353806681758936 35.0 31.0 38.0 7.5 40.0 72-73 30.877524867942647 35.0 30.5 37.0 4.5 39.5 74-75 30.326395005831102 34.5 30.0 37.0 2.0 39.0 76-77 29.775587569458736 34.0 29.0 36.0 2.0 39.0 78-79 29.237912236171137 34.0 29.0 36.0 2.0 38.0 80-81 28.67396675127484 34.0 28.5 35.0 2.0 37.0 82-83 28.119169925224668 34.0 27.0 35.0 2.0 37.0 84-85 27.57011456403924 33.0 26.0 35.0 2.0 36.0 86-87 26.959309414374243 33.0 25.5 35.0 2.0 36.0 88-89 26.387257094509614 32.0 24.5 35.0 2.0 35.0 90-91 25.789640758272164 32.0 23.0 34.5 2.0 35.0 92-93 25.092471244654824 32.0 19.5 34.0 2.0 35.0 94-95 24.282393267933504 31.0 12.0 34.0 2.0 35.0 96-97 23.349490292927214 31.0 2.0 34.0 2.0 35.0 98-99 22.12658846127461 30.5 2.0 34.0 2.0 35.0 100-101 18.9384619606229 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06653077018605202 1101 2 -0.02165576198157737 1101 3 -0.10276847885591422 1101 4 -0.05741977511462437 1101 5 -0.11233088353050391 1101 6 -0.02032392551097928 1101 7 0.006072441718650623 1101 8 0.042400635166274014 1101 9 0.0880020518740281 1101 10-11 0.15112172288644388 1101 12-13 0.045885374801777346 1101 14-15 0.2300521038418779 1101 16-17 0.3256066016025514 1101 18-19 0.410794008669221 1101 20-21 0.30657397493415317 1101 22-23 0.14202340104912992 1101 24-25 0.1320707253202542 1101 26-27 0.1461661834286332 1101 28-29 0.23098940119431433 1101 30-31 0.1401572164996736 1101 32-33 0.29753971241729005 1101 34-35 0.37947407039591496 1101 36-37 0.3742902518585538 1101 38-39 0.271827795672003 1101 40-41 0.46173542978247895 1101 42-43 0.40211116240241296 1101 44-45 0.45270151111061097 1101 46-47 0.4873153138526476 1101 48-49 0.5046235024059769 1101 50-51 0.486429802930445 1101 52-53 0.6479291566996963 1101 54-55 0.8088651429143852 1101 56-57 0.8056630019915829 1101 58-59 0.9318795366809951 1101 60-61 0.8985278296763468 1101 62-63 0.8506370421659497 1101 64-65 0.9755143582562269 1101 66-67 1.143734538755993 1101 68-69 1.0267997818997827 1101 70-71 1.0198643724016137 1101 72-73 0.8365996829535725 1101 74-75 0.8551677400607822 1101 76-77 0.9315869572972346 1101 78-79 1.0653603987501405 1101 80-81 1.27636404661002 1101 82-83 1.4268057047470855 1101 84-85 1.6096582502832497 1101 86-87 1.9631737301930556 1101 88-89 2.196936278136782 1101 90-91 2.205482838024192 1101 92-93 2.0345331393203203 1101 94-95 2.520823355232462 1101 96-97 2.774983814047026 1101 98-99 2.993480294438694 1101 100-101 3.4300760937233825 1102 1 -0.059834505151133754 1102 2 -0.1264746761512292 1102 3 -0.09270617711466045 1102 4 -0.023842103328412634 1102 5 -0.020068166444310975 1102 6 -0.03848134580810836 1102 7 -0.02332713380795326 1102 8 -0.0843325171192646 1102 9 0.13809128257304337 1102 10-11 0.20507799756180844 1102 12-13 0.11414708775733828 1102 14-15 0.11778094292994723 1102 16-17 0.15501781591596853 1102 18-19 0.21232120007021393 1102 20-21 0.2250000001743473 1102 22-23 0.18364530022527958 1102 24-25 0.12654891971082094 1102 26-27 0.049209612078300324 1102 28-29 0.21881774049918334 1102 30-31 0.23636822085851605 1102 32-33 0.197781616614094 1102 34-35 0.06685237661012167 1102 36-37 0.05869085433693044 1102 38-39 0.3088195115943151 1102 40-41 0.328419973017148 1102 42-43 0.37680570130105906 1102 44-45 0.219684098572678 1102 46-47 0.10862410684198665 1102 48-49 0.2727247826725261 1102 50-51 0.23213851376365113 1102 52-53 0.14602861127419686 1102 54-55 0.2774830940314814 1102 56-57 0.22191371515253877 1102 58-59 0.20654783125640108 1102 60-61 0.3224955149270343 1102 62-63 0.426525811877724 1102 64-65 0.3551026646986415 1102 66-67 0.5033203348718374 1102 68-69 0.498360520392211 1102 70-71 0.3633067147645228 1102 72-73 0.44850890041631786 1102 74-75 0.5075760560880767 1102 76-77 0.6259067729023329 1102 78-79 0.7858665718713915 1102 80-81 0.912679571838801 1102 82-83 1.165086131046177 1102 84-85 1.3539032125142434 1102 86-87 1.3922554070912057 1102 88-89 1.2441596287381174 1102 90-91 1.3569087035163285 1102 92-93 1.3325543173481158 1102 94-95 1.2916809740736035 1102 96-97 1.3867554199713972 1102 98-99 1.8665552162762573 1102 100-101 2.2402054896475256 1103 1 -0.012676748879403732 1103 2 -0.018321899678429787 1103 3 0.05335320463860427 1103 4 0.06530287459134598 1103 5 0.08597148403018195 1103 6 0.1261883901685863 1103 7 0.05474953630385926 1103 8 -0.04582927555203753 1103 9 0.03932495728881946 1103 10-11 0.08883304782958845 1103 12-13 0.15969157709306359 1103 14-15 0.17107136856181882 1103 16-17 0.3122371149857841 1103 18-19 0.1400422373717234 1103 20-21 0.07886216309833571 1103 22-23 7.541980924230529E-4 1103 24-25 0.052121596587639374 1103 26-27 0.07497819236581904 1103 28-29 0.028183051031930972 1103 30-31 -0.02705750497067072 1103 32-33 0.2725819548645845 1103 34-35 0.30519588877773884 1103 36-37 0.2839797766332808 1103 38-39 0.2601908354691673 1103 40-41 0.34588450369459167 1103 42-43 0.44751367837519496 1103 44-45 0.4061662934425456 1103 46-47 0.34307538937510174 1103 48-49 0.5359766853315477 1103 50-51 0.49761864154717017 1103 52-53 0.40247979984884097 1103 54-55 0.5281981179908897 1103 56-57 0.5868667359173614 1103 58-59 0.4002380501796452 1103 60-61 0.5040956276772022 1103 62-63 0.5859484370773771 1103 64-65 0.6403291046121993 1103 66-67 0.6279628228921297 1103 68-69 0.6954111640077372 1103 70-71 0.762115791152759 1103 72-73 0.7812275887560709 1103 74-75 0.8289154340830045 1103 76-77 1.0769262223057012 1103 78-79 0.9749067860967067 1103 80-81 1.1997936374218128 1103 82-83 1.28025619587752 1103 84-85 1.366924447128543 1103 86-87 1.303362707664352 1103 88-89 1.4540595629671884 1103 90-91 1.665514359816246 1103 92-93 1.8946932819257718 1103 94-95 1.7584553797862803 1103 96-97 1.8832939899234802 1103 98-99 2.096148376099613 1103 100-101 2.636926808824448 1104 1 0.17744685416920447 1104 2 0.19985120852548022 1104 3 0.18743280101531923 1104 4 0.12427288679557336 1104 5 0.08319591580028174 1104 6 0.13797233211483473 1104 7 0.2317676997276621 1104 8 0.1776288831329893 1104 9 0.3971584156731609 1104 10-11 0.23017759062242504 1104 12-13 0.4206857232015224 1104 14-15 0.43992915453842585 1104 16-17 0.48989252142035866 1104 18-19 0.3543197246174614 1104 20-21 0.2262472337004482 1104 22-23 0.24387780302150475 1104 24-25 0.30814002891811754 1104 26-27 0.24584605576122698 1104 28-29 0.2694983525740966 1104 30-31 0.3917707576853431 1104 32-33 0.3488893960577073 1104 34-35 0.47248122885066124 1104 36-37 0.3751457221411272 1104 38-39 0.4510588681065357 1104 40-41 0.6771801167548119 1104 42-43 0.548368491334621 1104 44-45 0.645561873970685 1104 46-47 0.5506847431904731 1104 48-49 0.6269555244444973 1104 50-51 0.5595012609614756 1104 52-53 0.6207441106673528 1104 54-55 0.6475640059564682 1104 56-57 0.7187793299641854 1104 58-59 0.6479887774499744 1104 60-61 0.6709508110884812 1104 62-63 0.708864880438675 1104 64-65 0.8900228236667758 1104 66-67 1.0411059658658601 1104 68-69 0.988189891420987 1104 70-71 1.0983748949601377 1104 72-73 1.0942634041168482 1104 74-75 1.2185558043092044 1104 76-77 1.1906220343192473 1104 78-79 1.3130496470004935 1104 80-81 1.626563344238317 1104 82-83 1.5047046551174752 1104 84-85 1.6467060967863922 1104 86-87 1.798531554731671 1104 88-89 2.0301947064373635 1104 90-91 2.120480013099634 1104 92-93 2.3661706044426616 1104 94-95 2.5327773306572183 1104 96-97 2.7932074320605906 1104 98-99 2.9394966027258853 1104 100-101 3.263942668677057 1105 1 0.016283560537626585 1105 2 0.008016274364603504 1105 3 -0.01705504944909464 1105 4 0.036824512138906584 1105 5 -0.08179460834585228 1105 6 -0.015332074118788341 1105 7 -0.04178398556025087 1105 8 -0.007901111441263708 1105 9 0.08983026831402441 1105 10-11 0.04697774924499498 1105 12-13 -0.03806395594129697 1105 14-15 0.07335194362114095 1105 16-17 0.09009218296407084 1105 18-19 0.10456603533631892 1105 20-21 0.13119588886697642 1105 22-23 0.07081704842690328 1105 24-25 0.07007399458986185 1105 26-27 0.21877108969138703 1105 28-29 0.2659070577559177 1105 30-31 0.10152220288755132 1105 32-33 0.12828106622114177 1105 34-35 0.2887449603850385 1105 36-37 0.21788881660302195 1105 38-39 0.24556579324191574 1105 40-41 0.20696872759309315 1105 42-43 0.24297624631808645 1105 44-45 0.2768335750593778 1105 46-47 0.42022306237535645 1105 48-49 0.5309842609512003 1105 50-51 0.4815133697654872 1105 52-53 0.4187562563603322 1105 54-55 0.5796106827983465 1105 56-57 0.7068449800323506 1105 58-59 0.34220996678106985 1105 60-61 0.6676384890969658 1105 62-63 0.7552258605171005 1105 64-65 0.750966781637942 1105 66-67 0.803165968847722 1105 68-69 0.7278067994798434 1105 70-71 0.676016952073482 1105 72-73 0.5486740183028083 1105 74-75 0.7195986861984096 1105 76-77 0.8620304705190058 1105 78-79 0.9595803163354617 1105 80-81 1.235627886100378 1105 82-83 1.328817283287428 1105 84-85 1.569903263713588 1105 86-87 1.765675011320269 1105 88-89 2.005018753741318 1105 90-91 1.8979088827140345 1105 92-93 1.956630041178851 1105 94-95 1.9638472183456805 1105 96-97 2.141947289515464 1105 98-99 2.1741150555103097 1105 100-101 2.956391650464667 1106 1 0.006112476313091975 1106 2 0.006297406276026152 1106 3 -0.05762903100303163 1106 4 0.13531498479419213 1106 5 0.07550188636712107 1106 6 0.09770691141462606 1106 7 -0.05664182781229954 1106 8 0.05131623508120953 1106 9 0.1604760811647381 1106 10-11 -0.13382694186037014 1106 12-13 -0.20260293813061026 1106 14-15 -0.2759536537356837 1106 16-17 -0.05328030979594445 1106 18-19 0.10210477675033758 1106 20-21 0.13810396620935705 1106 22-23 0.1225895893419846 1106 24-25 -0.18065000335017345 1106 26-27 -0.15289818537431898 1106 28-29 0.11406730040391722 1106 30-31 0.15719145788865063 1106 32-33 0.23810469831649073 1106 34-35 0.1101950296079437 1106 36-37 -0.23704022156655924 1106 38-39 -0.3309179845224932 1106 40-41 0.18668340886679857 1106 42-43 0.15769831805575052 1106 44-45 0.1997394988004899 1106 46-47 0.4043536294680621 1106 48-49 0.4766141893205571 1106 50-51 0.3489832895739369 1106 52-53 0.28514686746141393 1106 54-55 0.5418783513577736 1106 56-57 0.3270762915220118 1106 58-59 0.046980160137010785 1106 60-61 0.5181287343533612 1106 62-63 0.4988971487318139 1106 64-65 0.6454319595106952 1106 66-67 0.8318959794819634 1106 68-69 0.6702865843872416 1106 70-71 0.7149790767817095 1106 72-73 0.6259837890981004 1106 74-75 0.830203165956565 1106 76-77 1.2082227095286164 1106 78-79 1.1738669860787034 1106 80-81 1.1144627343116191 1106 82-83 1.1423042111952917 1106 84-85 1.189881259526775 1106 86-87 1.5806249835642312 1106 88-89 1.7072599704591482 1106 90-91 1.8771411773694915 1106 92-93 2.06474650954031 1106 94-95 2.175042266400414 1106 96-97 2.1468739868141 1106 98-99 2.21878054505331 1106 100-101 2.801114156935501 1107 1 0.009299864160002613 1107 2 0.0915877463483099 1107 3 0.11598337252520707 1107 4 0.05990905093378274 1107 5 0.0021511829475571176 1107 6 0.04295495211025724 1107 7 0.0014893714818668968 1107 8 -0.017587404595190037 1107 9 0.1323708910473087 1107 10-11 0.043877828709746325 1107 12-13 0.09804229394155328 1107 14-15 0.3430024293558276 1107 16-17 0.23488247042880772 1107 18-19 0.28889983500675953 1107 20-21 0.26167598888236654 1107 22-23 0.2981029948668663 1107 24-25 0.2129674090124567 1107 26-27 0.24044724498335768 1107 28-29 0.3162757253552613 1107 30-31 0.1621474467773112 1107 32-33 0.18063521659299653 1107 34-35 0.2567051587117888 1107 36-37 0.31971900514294305 1107 38-39 0.3749942207202466 1107 40-41 0.3628829442859143 1107 42-43 0.5087957812419646 1107 44-45 0.38062993399467615 1107 46-47 0.6683473477463977 1107 48-49 0.9300234133877723 1107 50-51 0.5338621240319625 1107 52-53 0.6130328061927983 1107 54-55 0.7092776749353646 1107 56-57 0.6660862080950523 1107 58-59 0.6576986636443394 1107 60-61 0.7611921880136023 1107 62-63 1.026855864907823 1107 64-65 0.9065191752650819 1107 66-67 1.0778782854298399 1107 68-69 1.2359844551730959 1107 70-71 1.3860859143001818 1107 72-73 1.4239132219739794 1107 74-75 1.4811649540644893 1107 76-77 1.3814195291485447 1107 78-79 1.3233749485170385 1107 80-81 1.4403879326545663 1107 82-83 1.545602976678449 1107 84-85 1.6632431064540363 1107 86-87 1.871415104212808 1107 88-89 1.8684674671677968 1107 90-91 2.11002296887791 1107 92-93 2.1428297572869077 1107 94-95 2.299219427494176 1107 96-97 2.4090982424392777 1107 98-99 2.544417390774317 1107 100-101 3.0665326932989494 1108 1 -0.0901380295118841 1108 2 -0.0144467421276957 1108 3 0.15061112404563204 1108 4 -0.0388378914863452 1108 5 -0.04531041529624247 1108 6 -0.0240371730954152 1108 7 0.07554354905392557 1108 8 0.02705793422716596 1108 9 0.04847578299422395 1108 10-11 0.044987510890258875 1108 12-13 0.08860049934941827 1108 14-15 0.027938003879377504 1108 16-17 -0.009084518259733443 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54-55 -0.40986717820081964 2316 56-57 -0.46231495998357985 2316 58-59 -0.646464872917818 2316 60-61 -0.4512975885212356 2316 62-63 -0.6446720102638395 2316 64-65 -0.7841350848476338 2316 66-67 -0.5574963620428655 2316 68-69 -0.631004270896355 2316 70-71 -1.0154591654532652 2316 72-73 -1.1971353827052802 2316 74-75 -1.3000827460897817 2316 76-77 -1.1102093795477543 2316 78-79 -0.8773639181467594 2316 80-81 -1.3633458365096551 2316 82-83 -1.9049501665944781 2316 84-85 -1.6007791831300402 2316 86-87 -1.7383616527999024 2316 88-89 -1.69888937307676 2316 90-91 -2.277519608643381 2316 92-93 -2.1251770801110474 2316 94-95 -2.2620213852730373 2316 96-97 -2.251865269683776 2316 98-99 -2.427147143070389 2316 100-101 -2.948977813437775 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4459.0 3 862.0 4 816.0 5 879.0 6 970.0 7 1228.0 8 1437.0 9 1844.0 10 2315.0 11 2952.0 12 4113.0 13 5473.0 14 6224.0 15 8100.0 16 10545.0 17 13940.0 18 12177.0 19 8322.0 20 7779.0 21 8216.0 22 8960.0 23 9825.0 24 11174.0 25 12744.0 26 14947.0 27 17416.0 28 20851.0 29 24794.0 30 30346.0 31 37703.0 32 47908.0 33 62355.0 34 84775.0 35 118765.0 36 161615.0 37 196457.0 38 120573.0 39 9416.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.279932453515904 18.78416328628329 14.921073401736384 22.014830858464418 2 15.28146617241414 19.92870581608999 41.05433200230064 23.735496009195227 3 19.199744605197612 25.140150649908215 29.780189839800308 25.87991490509386 4 12.526407037595916 16.591279141765543 37.11010920565613 33.77220461498241 5 13.54619024292694 37.43562647116561 33.64458289989561 15.37360038601184 6 31.26537129908618 35.97216276641675 17.61542854471438 15.147037389782685 7 28.484379640187274 33.45270262838539 20.081630632843638 17.9812870985837 8 26.870658504793177 34.365117340681216 19.41057413610089 19.353650018424716 9 26.85901701856955 16.800864586598905 20.46912439523854 35.870993999593004 10-11 25.106973145798804 26.923224997433376 26.521044468562543 21.448757388205273 12-13 26.210892023943757 24.419785679582727 27.65691316264701 21.712409133826508 14-15 22.985372328236227 25.364545999527017 26.641694456004238 25.00838721623252 16-17 21.63409077681227 29.101943004528348 26.976998056399697 22.28696816225969 18-19 21.53320780154552 26.68176775275034 30.553349558675873 21.231674887028266 20-21 22.448252001411415 25.6748892178113 30.60530379752636 21.271554983250923 22-23 22.081085440990194 25.730003611292894 30.68466996999144 21.504240977725473 24-25 21.787179790347402 25.933762463990877 30.844155546573326 21.434902199088395 26-27 21.470759023814338 26.2275306942827 30.71898570113217 21.582724580770797 28-29 21.27109958951249 26.35214291345295 30.94427710015052 21.432480396884035 30-31 21.403935275899702 25.973288451992506 30.90134401098566 21.721432261122136 32-33 21.2546755968791 26.133475600729124 30.824632976244004 21.787215826147776 34-35 21.91238474481263 26.22155487996319 30.37543960483805 21.490620770386137 36-37 21.93764664906878 25.898729652441705 30.443200982548763 21.720422715940753 38-39 21.550112795547015 26.170445687901616 30.559230378317594 21.720211138233772 40-41 21.64103499327114 26.122999957386188 30.599695471618055 21.636269577724615 42-43 21.75614182213118 26.01447183308358 30.241411072464565 21.98797527232067 44-45 21.620662980994837 26.452653903683714 29.964750263960582 21.961932851360864 46-47 21.887440133825248 26.696578748367283 29.484406150461744 21.931574967345725 48-49 22.056333664254883 26.723610839926554 29.185538820030928 22.034516675787636 50-51 21.938381029683857 27.029556444793577 28.95343630508004 22.078626220442523 52-53 22.186851845485695 27.073005832674436 28.74167783927487 21.998464482565 54-55 21.775396608973228 27.01569936776302 28.83972253985568 22.369181483408077 56-57 21.92473258693645 27.351253273218372 28.755936466917298 21.968077672927876 58-59 22.097329033741776 26.8400888199472 28.91064542891999 22.151936717391035 60-61 22.07477127105846 26.80472266615838 29.23657133850417 21.88393472427899 62-63 21.837864485568108 27.045249517669273 29.1796460327009 21.93723996406172 64-65 21.805331549430008 26.894908544408196 29.07339357061691 22.226366335544885 66-67 21.78877434135166 27.251775486827036 28.744650630011453 22.214799541809853 68-69 21.710458191335043 27.06747832111056 28.98194670608255 22.240116781471848 70-71 21.76048429331966 27.146240582383612 28.819567106945815 22.273708017350913 72-73 21.953690635482157 27.113065234525358 28.699479443517788 22.233764686474697 74-75 21.70585477646761 27.48038990599711 28.540024663556913 22.27373065397836 76-77 21.89870401261159 27.286858651219916 28.50104485546166 22.31339248070684 78-79 21.997880104464198 27.299681099156214 28.457237546071983 22.245201250307606 80-81 21.743908066413383 27.7098948755677 28.381717944288315 22.164479113730604 82-83 21.91281645489872 27.45045931292523 28.415637303842978 22.221086928333072 84-85 21.884627297035273 27.313712661436195 28.33153730695007 22.470122734578457 86-87 21.99049987239387 27.097585930244115 28.47999332874226 22.431920868619752 88-89 21.96826464579998 27.0787032751714 28.4002459792384 22.55278609979022 90-91 21.798411818344277 27.26471679870966 28.509327016042192 22.42754436690387 92-93 22.02526402412678 27.052226581704865 28.42186677394568 22.500642620222674 94-95 21.89308308372979 26.997183454824626 28.367700499573644 22.742032961871942 96-97 21.977336640564104 26.92452741135569 28.24978726465651 22.848348683423687 98-99 21.9646032865431 27.09988988646346 27.947530418692633 22.98797640830081 100-101 22.186715414078108 26.27558780948192 28.803626734106697 22.73407004233328 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1777.0 1 1102.5 2 611.0 3 720.0 4 515.0 5 424.0 6 780.5 7 1776.0 8 2936.0 9 3309.0 10 2626.0 11 1905.5 12 1816.0 13 1916.5 14 2077.0 15 2219.0 16 2232.0 17 2260.5 18 2384.0 19 2511.5 20 2686.0 21 3045.5 22 3520.0 23 4147.0 24 4969.0 25 6014.0 26 7331.0 27 8619.0 28 9670.5 29 11001.0 30 12736.5 31 14649.5 32 16701.5 33 18688.0 34 20845.5 35 22525.5 36 24573.0 37 26909.0 38 28231.0 39 28922.0 40 30123.5 41 32908.0 42 35536.0 43 37841.5 44 39565.0 45 40718.0 46 40718.5 47 40656.0 48 42706.0 49 42814.5 50 40414.5 51 38087.0 52 36459.5 53 35717.5 54 34534.5 55 31927.0 56 28547.5 57 25338.5 58 22878.0 59 20356.0 60 17399.5 61 14627.5 62 11963.5 63 9339.5 64 7290.0 65 5781.0 66 4394.5 67 3477.0 68 2575.5 69 1728.0 70 1363.5 71 1091.0 72 881.5 73 736.0 74 558.5 75 439.0 76 335.5 77 207.5 78 138.0 79 94.0 80 61.0 81 37.5 82 24.0 83 18.5 84 12.5 85 9.5 86 9.5 87 8.5 88 7.0 89 4.5 90 3.0 91 3.0 92 2.0 93 2.5 94 3.0 95 3.0 96 2.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33431661750245817 2 0.2875763188584757 3 0.29251560677780064 4 0.28629576273124324 5 0.2883995335116965 6 0.216048112323066 7 0.20699275113763693 8 0.21485902449063593 9 0.21485902449063593 10-11 0.2146760879010313 12-13 0.21815188310351924 14-15 0.21302965859458967 16-17 0.2065811438110265 18-19 0.19972102170085293 20-21 0.1984861997210217 22-23 0.20607806818961374 24-25 0.2041572339987652 26-27 0.21170336831995612 28-29 0.21742013674510072 30-31 0.21938670508335048 32-33 0.216917061123688 34-35 0.2177402757769088 36-37 0.20443163888317212 38-39 0.19587935331915576 40-41 0.19020831904141228 42-43 0.20031556561706798 44-45 0.20150465344949808 46-47 0.21037707804532255 48-49 0.21787747821911232 50-51 0.2129839244471885 52-53 0.22295396858064073 54-55 0.2110630902563399 56-57 0.1859093091857035 58-59 0.16953648441608926 60-61 0.18540623356429078 62-63 0.17923212366513458 64-65 0.1861379799227093 66-67 0.18522329697468615 68-69 0.1834853993734422 70-71 0.18618371407011045 72-73 0.19528480940294068 74-75 0.20045276805927145 76-77 0.20260227298712583 78-79 0.19972102170085293 80-81 0.1956964167295511 82-83 0.1852690311220873 84-85 0.18083281882417507 86-87 0.18536049941688962 88-89 0.19446159474971988 90-91 0.19116873613683655 92-93 0.1865495872493197 94-95 0.18700692872333127 96-97 0.1948732020763303 98-99 0.19469026548672566 100-101 0.17699115044247787 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1093275.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.50533511260768 #Duplication Level Percentage of deduplicated Percentage of total 1 80.26277197388313 46.95800371398848 2 11.403060528710917 13.34279755083163 3 3.4631625693929213 6.078404600153169 4 1.516146667978293 3.548106755597342 5 0.8056115042362808 2.3566285512957785 6 0.5200912685194687 1.8256868372323647 7 0.35491142663710407 1.4534948365488214 8 0.25081191173277867 1.1739067956927993 9 0.19468415087150398 1.025105533906573 >10 1.1004151364678538 11.932278894493297 >50 0.08489284724758298 3.431544405535942 >100 0.040783611787545825 4.126601206088865 >500 0.0017188486988850113 0.6829975916055286 >1k 6.250358905036405E-4 0.784747868604184 >5k 3.1251794525182023E-4 1.279694858425136 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7883 0.7210445679266425 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6115 0.559328622716151 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4262 0.3898378724474629 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1579 0.14442843749285403 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1563 0.142964944776017 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1180 0.1079325878667307 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 9.146829480231415E-5 0.0 0.0 0.0 0.0 12-13 9.146829480231415E-5 0.0 0.0 9.146829480231415E-5 0.0 14-15 9.146829480231415E-5 0.0 0.0 9.146829480231415E-5 9.146829480231415E-5 16-17 9.146829480231415E-5 0.0 0.0 9.146829480231415E-5 9.146829480231415E-5 18-19 9.146829480231415E-5 0.0 0.0 1.829365896046283E-4 9.146829480231415E-5 20-21 9.146829480231415E-5 0.0 0.0 1.829365896046283E-4 9.146829480231415E-5 22-23 1.829365896046283E-4 0.0 0.0 3.2013903180809955E-4 9.146829480231415E-5 24-25 2.7440488440694244E-4 0.0 0.0 5.030756214127278E-4 9.146829480231415E-5 26-27 2.7440488440694244E-4 0.0 0.0 6.402780636161991E-4 9.146829480231415E-5 28-29 3.658731792092566E-4 0.0 0.0 0.001234821979831241 9.146829480231415E-5 30-31 4.573414740115707E-4 0.0 0.0 0.0025611122544647964 9.146829480231415E-5 32-33 4.573414740115707E-4 0.0 0.0 0.004802085477121493 9.146829480231415E-5 34-35 5.030756214127278E-4 0.0 0.0 0.008369348974411744 9.146829480231415E-5 36-37 5.488097688138849E-4 0.0 0.0 0.012622624682719352 9.146829480231415E-5 38-39 5.488097688138849E-4 0.0 0.0 0.018385127255265143 9.146829480231415E-5 40-41 5.488097688138849E-4 0.0 0.0 0.0258855274290549 9.146829480231415E-5 42-43 5.945439162150419E-4 0.0 0.0 0.036312913036518714 9.146829480231415E-5 44-45 6.40278063616199E-4 0.0 0.0 0.04838672795042419 9.146829480231415E-5 46-47 6.40278063616199E-4 0.0 0.0 0.06393633806681759 9.146829480231415E-5 48-49 6.40278063616199E-4 0.0 0.0 0.08328188241750703 9.146829480231415E-5 50-51 6.40278063616199E-4 0.0 0.0 0.108024056161533 9.146829480231415E-5 52-53 6.40278063616199E-4 0.0 0.0 0.1367908348768608 9.146829480231415E-5 54-55 6.40278063616199E-4 0.0 0.0 0.1709999771329263 9.146829480231415E-5 56-57 6.40278063616199E-4 0.0 0.0 0.2107429512245318 9.146829480231415E-5 58-59 6.40278063616199E-4 0.0 0.0 0.25405318881342753 9.146829480231415E-5 60-61 6.40278063616199E-4 0.0 0.0 0.31044339255905423 9.146829480231415E-5 62-63 6.40278063616199E-4 0.0 0.0 0.3671080011890878 9.146829480231415E-5 64-65 6.40278063616199E-4 0.0 0.0 0.4303583270448881 9.146829480231415E-5 66-67 7.317463584185132E-4 0.0 0.0 0.508655187395669 9.146829480231415E-5 68-69 7.317463584185132E-4 0.0 0.0 0.604468226201093 9.146829480231415E-5 70-71 7.317463584185132E-4 0.0 0.0 0.7196268093572066 1.829365896046283E-4 72-73 7.774805058196703E-4 0.0 0.0 0.8221627678306007 1.829365896046283E-4 74-75 8.232146532208273E-4 0.0 0.0 0.9367725412179003 1.829365896046283E-4 76-77 8.232146532208273E-4 0.0 0.0 1.066566051542384 1.829365896046283E-4 78-79 8.689488006219843E-4 0.0 0.0 1.2067869474743316 1.829365896046283E-4 80-81 9.146829480231414E-4 0.0 0.0 1.3639752120921087 1.829365896046283E-4 82-83 9.146829480231414E-4 0.0 0.0 1.5582538702522237 1.829365896046283E-4 84-85 9.604170954242985E-4 0.0 0.0 1.753081338181153 1.829365896046283E-4 86-87 0.0010061512428254555 0.0 0.0 1.9640529601426906 1.829365896046283E-4 88-89 0.0010061512428254555 0.0 0.0 2.2055292584207997 1.829365896046283E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3695 0.0 52.892773 1 GTATCAA 6395 0.0 40.227745 1 TATCAAC 8115 0.0 31.158237 2 ATCAACG 8195 0.0 30.96864 3 TCAACGC 8270 0.0 30.634539 4 CAACGCA 8390 0.0 30.197762 5 AACGCAG 8630 0.0 29.39821 6 GTACATG 6115 0.0 26.43939 1 ACGCAGA 9790 0.0 25.91487 7 CGCAGAG 9880 0.0 25.678802 8 TACATGG 6370 0.0 25.069729 2 GCAGAGT 10410 0.0 24.096487 9 ACATGGG 6275 0.0 23.781857 3 GAGTACT 6085 0.0 23.76991 12-13 GTACTTT 6440 0.0 22.311583 14-15 CATGGGA 3665 0.0 22.047764 4 ATAACGC 130 2.9305747E-4 21.935005 3 TGGTATC 1685 0.0 21.719038 2 AGTACTT 6255 0.0 21.225374 12-13 CAGAGTA 10345 0.0 21.21496 10-11 >>END_MODULE