FastQCFastQC Report
Thu 26 May 2016
SRR937564_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937564_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093275
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77630.7100683725503648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65590.5999405456083785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45110.41261347785323915No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17960.16427705746495622No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12550.11479270997690426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40950.045.8476941
GTATCAA69100.038.7261771
TATCAAC88050.030.4841982
ATCAACG87550.030.3869843
TCAACGC89950.029.5762144
CAACGCA90950.029.2510225
TCGCGTA650.005842477529.2322759
AACGCAG92050.028.9477836
CGTATAT700.00838554327.146742
ACGCAGA105050.025.1417057
CGCAGAG106500.024.7993988
GAGTACT63350.024.29485912-13
TAAGGTG3550.024.087955
GTACATG60750.023.4719561
GCAGAGT113200.022.9958849
GTACTTT66750.022.87736314-15
TACATGG62650.021.9903742
AGTACTT63950.021.91277712-13
GGTGCGT1353.7944617E-421.1121987
ACATGGG62700.020.9878483
Produced by FastQC (version 0.11.5)