FastQCFastQC Report
Thu 26 May 2016
SRR937563_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937563_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622848
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65101.0451988286066585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47350.7602175811755035No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35900.5763846074804768No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34290.550535604192355No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29250.4696169852034525No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24010.3854873099054665No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC18680.29991265926839294No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT13950.22397117755856966No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC11770.1889706637895602No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT11540.18527794903411426No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11230.18030081175503493No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC10130.16264000205507603No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC9280.14899301274147145No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9080.14578195643238798No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8610.13823597410604194No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8290.13309828401150844No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG8000.12844225236333745No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA7670.12314400945334977No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA6450.10355656596794081No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT6410.10291435470612413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG45150.026.3236372
GTACATG46200.026.1538221
ACATGGG45350.025.050383
GAGTACT42500.024.69521312-13
TAGCCCT3850.024.6923834
CATGGGT4750.024.0165924
AGTACTT43550.023.71813412-13
GTATCAA89600.023.6793771
GTAGCAC1652.4123674E-623.0462253
GTACTTT45950.022.99608414-15
CATGGGA32950.022.0713944
ATGGGCG1100.002864385521.6058355
AGAGTAC71100.021.54118210-11
CATGGGG12300.020.8680744
TAGACAG2308.938332E-820.666455
ACTTTTT52050.020.48354116-17
TATCAAC102850.020.4768472
TAAGGTG2108.5377906E-720.3712165
TACTTTT51300.020.27384814-15
TCAACGC103900.020.2666474
Produced by FastQC (version 0.11.5)