##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937563_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 622848 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.899866420057542 33.0 31.0 34.0 30.0 34.0 2 32.01987162196876 33.0 31.0 34.0 30.0 34.0 3 32.02946946927661 34.0 31.0 34.0 30.0 34.0 4 35.60772130600082 37.0 35.0 37.0 33.0 37.0 5 35.61821985460337 37.0 35.0 37.0 35.0 37.0 6 35.70972853729963 37.0 35.0 37.0 35.0 37.0 7 35.66485402538019 37.0 35.0 37.0 35.0 37.0 8 35.66013698366215 37.0 35.0 37.0 33.0 37.0 9 37.30127254161529 39.0 37.0 39.0 34.0 39.0 10-11 37.18900036606042 39.0 37.0 39.0 34.0 39.0 12-13 37.086874325678174 39.0 37.0 39.0 33.5 39.0 14-15 38.20682895345253 40.0 38.0 41.0 33.5 41.0 16-17 38.15783144523222 40.0 38.0 41.0 33.5 41.0 18-19 38.17621072878134 40.0 38.0 41.0 33.5 41.0 20-21 38.128840423345665 40.0 38.0 41.0 34.0 41.0 22-23 38.05196693254213 40.0 38.0 41.0 33.5 41.0 24-25 38.04924797061241 40.0 38.0 41.0 33.0 41.0 26-27 37.94768868166872 40.0 38.0 41.0 33.0 41.0 28-29 37.82011983662146 40.0 38.0 41.0 33.0 41.0 30-31 37.54823729063913 40.0 37.0 41.0 33.0 41.0 32-33 37.32788657906905 40.0 36.0 41.0 33.0 41.0 34-35 37.290631101006994 40.0 36.0 41.0 33.0 41.0 36-37 37.127639488286064 40.0 36.0 41.0 32.0 41.0 38-39 36.956811292642826 40.0 36.0 41.0 31.5 41.0 40-41 36.735494855887794 39.5 35.5 41.0 31.0 41.0 42-43 36.408862194307446 39.0 35.0 41.0 30.0 41.0 44-45 36.05634922163995 39.0 35.0 41.0 29.5 41.0 46-47 35.5499511919441 39.0 35.0 41.0 27.5 41.0 48-49 35.10623378416564 39.0 35.0 40.5 24.5 41.0 50-51 34.15542074470818 38.0 34.0 39.5 23.5 40.5 52-53 34.24136386405672 38.0 34.5 40.0 23.0 40.5 54-55 34.5196476507912 39.0 35.0 40.0 23.0 41.0 56-57 34.29088718274764 38.0 34.0 40.0 21.5 41.0 58-59 34.05775486153925 38.0 34.0 40.0 20.5 41.0 60-61 33.710771327836 38.0 34.0 40.0 18.5 41.0 62-63 33.36741548499795 37.5 33.0 40.0 16.5 41.0 64-65 33.00196195540485 37.0 33.0 40.0 12.5 41.0 66-67 32.54726032675709 36.0 32.0 39.0 8.0 41.0 68-69 32.06069859741061 36.0 32.0 39.0 7.0 40.5 70-71 31.586947377209206 35.0 31.0 38.5 2.0 40.0 72-73 31.133055737515413 35.0 31.0 38.0 2.0 40.0 74-75 30.640334399404026 35.0 30.5 37.0 2.0 39.0 76-77 30.17618905415125 35.0 30.0 36.5 2.0 39.0 78-79 29.625618128339497 34.0 29.0 36.0 2.0 39.0 80-81 29.053526703144264 34.0 29.0 36.0 2.0 37.5 82-83 28.55884421239211 34.0 28.5 35.0 2.0 37.0 84-85 28.033351636354293 34.0 27.0 35.0 2.0 36.5 86-87 27.559346742704477 33.5 26.0 35.0 2.0 36.0 88-89 27.12273781083025 33.0 26.0 35.0 2.0 36.0 90-91 26.583906506884503 33.0 25.0 35.0 2.0 35.0 92-93 25.98958975544595 32.5 23.5 35.0 2.0 35.0 94-95 25.276433415536374 32.0 19.5 34.5 2.0 35.0 96-97 24.506160411528974 32.0 12.5 34.0 2.0 35.0 98-99 23.5635243269626 31.0 2.0 34.0 2.0 35.0 100-101 20.897105714395806 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08375436817786053 1101 2 0.206253019230342 1101 3 0.21894753013296508 1101 4 0.15957733831078968 1101 5 0.07400477884031176 1101 6 0.15424657257987207 1101 7 0.26593965117537266 1101 8 0.25622843717876265 1101 9 0.2055612283777677 1101 10-11 0.2069026100925484 1101 12-13 0.10839684608158251 1101 14-15 0.30203218295576306 1101 16-17 0.35479139697964257 1101 18-19 0.2634872568352691 1101 20-21 0.26817462271313275 1101 22-23 0.3021791800061777 1101 24-25 0.3118574362509179 1101 26-27 0.27591002155883615 1101 28-29 0.19155545253294548 1101 30-31 0.3137187962065937 1101 32-33 0.13546021986681467 1101 34-35 0.18261948721707455 1101 36-37 0.4235574252112997 1101 38-39 0.3825634648371974 1101 40-41 0.3874915075911147 1101 42-43 0.4216461838711183 1101 44-45 0.5869419113113779 1101 46-47 0.5177193053990763 1101 48-49 0.4369117542499126 1101 50-51 0.3437146669910547 1101 52-53 0.32817802142925956 1101 54-55 0.4656545921896367 1101 56-57 0.3347418153078294 1101 58-59 0.41571454043877765 1101 60-61 0.6704459994461303 1101 62-63 0.6068528845608725 1101 64-65 0.50443205813405 1101 66-67 0.591357324084079 1101 68-69 0.5513723944272222 1101 70-71 0.7217511509324908 1101 72-73 0.5999836044619009 1101 74-75 0.5812465878975139 1101 76-77 0.5814870551708289 1101 78-79 0.5608463631661955 1101 80-81 0.5603161115088149 1101 82-83 0.6863107133001165 1101 84-85 0.7938740899475967 1101 86-87 0.8309159225020899 1101 88-89 0.7329322189293457 1101 90-91 0.9108327877459992 1101 92-93 1.055054671258187 1101 94-95 0.9277238734023108 1101 96-97 1.0507450332255388 1101 98-99 1.2242175002098215 1101 100-101 1.5408436451740357 1102 1 0.08050890856137372 1102 2 0.2407468941497477 1102 3 0.07931670116433764 1102 4 -0.06970025715136074 1102 5 0.004493626195049671 1102 6 0.017361344492158537 1102 7 0.10587981301369354 1102 8 0.07066674809089335 1102 9 0.2062947167397482 1102 10-11 0.11120592292442666 1102 12-13 0.2419433438104832 1102 14-15 0.21598403714190084 1102 16-17 0.32076749793662174 1102 18-19 0.3064823524241902 1102 20-21 0.2511287153970798 1102 22-23 0.3465271541294399 1102 24-25 0.3877752927987004 1102 26-27 0.19290177751187088 1102 28-29 0.24785475925060751 1102 30-31 0.49707005925544223 1102 32-33 0.49052291953864113 1102 34-35 0.5575053890274333 1102 36-37 0.626970047731028 1102 38-39 0.7017866553847938 1102 40-41 0.7976174502594944 1102 42-43 0.7016521966645968 1102 44-45 0.7605921116170862 1102 46-47 0.6455771933872612 1102 48-49 0.8414523188729532 1102 50-51 0.5205885939247494 1102 52-53 0.5541431472270304 1102 54-55 0.5064917831284319 1102 56-57 0.6518560004294258 1102 58-59 0.8211497797552099 1102 60-61 0.7991786402215695 1102 62-63 0.86040079493403 1102 64-65 0.7873566301752817 1102 66-67 0.8719609940614106 1102 68-69 0.964191416587628 1102 70-71 1.0195238818273893 1102 72-73 0.8953702343273768 1102 74-75 0.9238617184041082 1102 76-77 0.9989795754006181 1102 78-79 1.1149343383036552 1102 80-81 0.9946516978286262 1102 82-83 0.9474198925900268 1102 84-85 0.9707661277050406 1102 86-87 0.8920390471262891 1102 88-89 0.7670321834321321 1102 90-91 0.9230910988030203 1102 92-93 1.0777348558436977 1102 94-95 1.1015035660978505 1102 96-97 1.2976453657402622 1102 98-99 1.2361027282975385 1102 100-101 1.4525407018029597 1103 1 0.08961781054509288 1103 2 0.20237705023809482 1103 3 0.11298590183007207 1103 4 0.026854103876033264 1103 5 0.012050641174127463 1103 6 -0.13632045119855007 1103 7 -0.12472159942839056 1103 8 0.00413802693574894 1103 9 0.0940865389699681 1103 10-11 0.22565196673712506 1103 12-13 0.1454452105731221 1103 14-15 0.1946512136071945 1103 16-17 0.23552616826184902 1103 18-19 0.21485354580280358 1103 20-21 0.2312915510769855 1103 22-23 0.21967725983768105 1103 24-25 0.14427522532535875 1103 26-27 0.08133459654835207 1103 28-29 -0.06534527673517232 1103 30-31 0.0964695464579961 1103 32-33 0.2028165635492556 1103 34-35 0.29374082602981844 1103 36-37 0.19787329094995698 1103 38-39 0.26278256200168215 1103 40-41 0.3171509845134608 1103 42-43 0.1916436500405183 1103 44-45 0.2808116249093402 1103 46-47 0.3323884029594737 1103 48-49 0.48640756155209175 1103 50-51 0.23596352082862637 1103 52-53 0.4663153392111994 1103 54-55 0.5929251419516959 1103 56-57 0.39326439411392045 1103 58-59 0.6220584208419808 1103 60-61 0.5777760942272323 1103 62-63 0.5776762211406776 1103 64-65 0.7121913204726198 1103 66-67 0.3757619926669591 1103 68-69 0.5798294895488034 1103 70-71 0.33421957702778116 1103 72-73 0.2163072142203184 1103 74-75 0.16712562915936147 1103 76-77 0.46790809863394145 1103 78-79 0.33932820272720576 1103 80-81 0.43674831966299976 1103 82-83 0.5579187455431125 1103 84-85 0.5441096570109671 1103 86-87 0.4551716097640508 1103 88-89 0.430688789072903 1103 90-91 0.6720677530246029 1103 92-93 0.4253462993235253 1103 94-95 0.4626996436472197 1103 96-97 0.5981266756228578 1103 98-99 0.7476069341362326 1103 100-101 1.0517490145194408 1104 1 0.27032670830825367 1104 2 0.2457591920698512 1104 3 0.22295640792461313 1104 4 0.2375423820061684 1104 5 0.23436682628101124 1104 6 0.20482224188213394 1104 7 0.10844207869652678 1104 8 0.2266473579722117 1104 9 0.3124484526807194 1104 10-11 0.179045070905417 1104 12-13 0.2946882873355676 1104 14-15 0.3551463458077464 1104 16-17 0.3460904675858316 1104 18-19 0.28919123796995194 1104 20-21 0.20287430366749248 1104 22-23 0.2316562225918375 1104 24-25 0.029713588283101444 1104 26-27 0.18221810867216703 1104 28-29 0.30985896203495855 1104 30-31 0.29066081312988246 1104 32-33 0.361087907479515 1104 34-35 0.09514896425965702 1104 36-37 0.3751838643845602 1104 38-39 0.4961367759760478 1104 40-41 0.4334652903626477 1104 42-43 0.6006862726490283 1104 44-45 0.7062233650497589 1104 46-47 0.8516664547956694 1104 48-49 0.9264213949767779 1104 50-51 0.7333664910998081 1104 52-53 1.034066494171661 1104 54-55 1.2308404709522947 1104 56-57 1.2439351847071904 1104 58-59 1.2047039980611984 1104 60-61 1.007819421557386 1104 62-63 0.9439800866693489 1104 64-65 1.0509953817573034 1104 66-67 1.2038555901403996 1104 68-69 1.0573475643537087 1104 70-71 1.2562982448428812 1104 72-73 1.1928987105792537 1104 74-75 0.9279104802542903 1104 76-77 0.7997628215739354 1104 78-79 1.0190583204418218 1104 80-81 1.401694943524351 1104 82-83 1.2072844960716544 1104 84-85 1.0288720092151777 1104 86-87 1.3535716306446908 1104 88-89 1.562086747990243 1104 90-91 1.4396335922958663 1104 92-93 1.3848091448328823 1104 94-95 1.5602422532053346 1104 96-97 1.4766744270388266 1104 98-99 1.589999855598922 1104 100-101 1.5249121706283297 1105 1 0.2735592569736056 1105 2 0.3193567973269502 1105 3 0.30396142058911835 1105 4 0.3403480571217443 1105 5 0.373040507135471 1105 6 0.24256246895760114 1105 7 0.22833332336060863 1105 8 0.30909948968307077 1105 9 0.27292456724578784 1105 10-11 0.41562955112930666 1105 12-13 0.5466534997981611 1105 14-15 0.35506309660924273 1105 16-17 0.5408811932708701 1105 18-19 0.5179821762061394 1105 20-21 0.6025678757666171 1105 22-23 0.6737085810239165 1105 24-25 0.516332608068069 1105 26-27 0.4943831192111716 1105 28-29 0.5184903097863369 1105 30-31 0.5805814607023692 1105 32-33 0.5124128096174303 1105 34-35 0.6428684675984826 1105 36-37 0.7754406970182401 1105 38-39 0.8204056885998128 1105 40-41 0.759529374532903 1105 42-43 0.695982692933498 1105 44-45 0.6438094925450244 1105 46-47 0.4037255440140086 1105 48-49 0.5986099632676911 1105 50-51 0.6913522147479014 1105 52-53 0.5002974309908339 1105 54-55 0.8194911254326982 1105 56-57 0.8270382542999855 1105 58-59 0.8357134388745564 1105 60-61 0.8092612268251074 1105 62-63 0.9799446260533031 1105 64-65 0.8130372897580784 1105 66-67 0.8902009503260331 1105 68-69 0.9369178567886571 1105 70-71 0.8316671096426447 1105 72-73 0.748031820733889 1105 74-75 0.5573399575556017 1105 76-77 0.6028802383690177 1105 78-79 0.6307849442440094 1105 80-81 1.0042021614069903 1105 82-83 0.9184035778507038 1105 84-85 0.671448660471885 1105 86-87 0.6273623031919371 1105 88-89 0.8153325811021652 1105 90-91 0.8873902920850902 1105 92-93 0.7013934479308155 1105 94-95 1.5204668489153441 1105 96-97 1.0818837013537923 1105 98-99 0.9531541145924898 1105 100-101 0.9884871037632799 1106 1 0.025708292495721707 1106 2 0.07693779568761627 1106 3 0.02419097582584584 1106 4 0.2226961265826617 1106 5 0.03446114810708423 1106 6 0.06419678337325507 1106 7 0.15501400432901136 1106 8 0.2738501918190792 1106 9 0.22085567144447538 1106 10-11 0.0850994841188637 1106 12-13 0.06885877927629735 1106 14-15 0.2556703559525815 1106 16-17 0.12436291094033436 1106 18-19 0.17187479872369238 1106 20-21 0.11416986634959159 1106 22-23 0.20020827775962857 1106 24-25 0.354229926919416 1106 26-27 0.303857631613333 1106 28-29 0.4521046599116687 1106 30-31 0.4718289365447461 1106 32-33 0.2604769116506418 1106 34-35 0.40079779362099544 1106 36-37 0.3394379883444003 1106 38-39 0.15292585141255444 1106 40-41 0.4561591217152241 1106 42-43 0.3604286692618288 1106 44-45 0.48884509260640385 1106 46-47 0.7529995366041291 1106 48-49 0.9431425586985114 1106 50-51 0.8078702534926521 1106 52-53 0.8345129508150606 1106 54-55 0.6549879064466424 1106 56-57 0.6068488907165417 1106 58-59 0.6614899294368328 1106 60-61 0.7442737593133586 1106 62-63 0.7455091482719851 1106 64-65 0.7828740629285704 1106 66-67 0.990017530235562 1106 68-69 0.7341339149374484 1106 70-71 0.3878185030750849 1106 72-73 0.7834382314243804 1106 74-75 0.6793179715033233 1106 76-77 0.7797393459194453 1106 78-79 0.7848310258013989 1106 80-81 0.6913625114884674 1106 82-83 0.4985408627949113 1106 84-85 0.6249262128245583 1106 86-87 0.7051258447342761 1106 88-89 0.9027981441628725 1106 90-91 0.9400632806146341 1106 92-93 0.923958990773901 1106 94-95 0.7164559655028064 1106 96-97 1.0646159055858533 1106 98-99 1.1956340126762015 1106 100-101 1.130591392578065 1107 1 0.11645739183731152 1107 2 0.2991682340967792 1107 3 0.24718749150528652 1107 4 0.2400201210692714 1107 5 0.1619316733580547 1107 6 0.14469405329865737 1107 7 0.33931918111834847 1107 8 0.10506177703703656 1107 9 0.08876797778073353 1107 10-11 0.2261952408198198 1107 12-13 0.410969958088792 1107 14-15 0.6309905407576366 1107 16-17 0.6515083709712144 1107 18-19 0.52961365806717 1107 20-21 0.4816414564183873 1107 22-23 0.293866400249172 1107 24-25 0.302815493905328 1107 26-27 0.3541392167721398 1107 28-29 0.3236757986160157 1107 30-31 0.43444495732052957 1107 32-33 0.5315765726836332 1107 34-35 0.5132824868647319 1107 36-37 0.5598227425193727 1107 38-39 0.4611107511126207 1107 40-41 0.6422922357749599 1107 42-43 0.711405935401217 1107 44-45 0.8152073199686711 1107 46-47 1.272294922985111 1107 48-49 1.2607333492074346 1107 50-51 1.356242561130351 1107 52-53 1.0712648423456201 1107 54-55 1.1883167044447944 1107 56-57 1.3328283752264056 1107 58-59 1.1721040926970687 1107 60-61 1.12121039673697 1107 62-63 1.4460749806341582 1107 64-65 1.2569294807808333 1107 66-67 1.2094262014020671 1107 68-69 1.47826854340477 1107 70-71 1.424001604416162 1107 72-73 1.203196813560286 1107 74-75 1.1694018554855035 1107 76-77 1.1024190782952346 1107 78-79 1.3520905396529024 1107 80-81 1.4495290726592955 1107 82-83 1.4055423357929087 1107 84-85 1.2755683571310392 1107 86-87 1.490090321228422 1107 88-89 1.5502680186918418 1107 90-91 1.7959268774509418 1107 92-93 1.975117571726674 1107 94-95 1.5796490583844935 1107 96-97 1.7979115759182491 1107 98-99 1.9720104376095016 1107 100-101 1.627142565948155 1108 1 0.043023111325545926 1108 2 0.08673762957743136 1108 3 0.22654853967653565 1108 4 0.11601869722118607 1108 5 -0.0541509645943421 1108 6 0.16785554368426148 1108 7 0.3217846288432682 1108 8 0.09496820009790241 1108 9 0.12714684458932624 1108 10-11 0.08432897301316444 1108 12-13 0.3291397656639319 1108 14-15 0.22452469599814862 1108 16-17 0.4277708381987466 1108 18-19 0.1535209573422449 1108 20-21 -0.07420008571862269 1108 22-23 0.2029300832239187 1108 24-25 0.05318856948564843 1108 26-27 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-0.7344071403966623 2316 76-77 -0.5874565948121457 2316 78-79 -0.3063906133404437 2316 80-81 -0.37834153234545553 2316 82-83 -1.017715503928347 2316 84-85 -0.8529040850609171 2316 86-87 -0.8548178194114264 2316 88-89 -0.6650035999334385 2316 90-91 -0.7602261158971721 2316 92-93 -0.8409063433826063 2316 94-95 -0.3461269948305805 2316 96-97 -0.20411962458855726 2316 98-99 -0.28984021174114716 2316 100-101 -0.25485775080825945 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2497.0 3 472.0 4 373.0 5 459.0 6 466.0 7 559.0 8 650.0 9 894.0 10 1132.0 11 1552.0 12 2281.0 13 3181.0 14 3706.0 15 5051.0 16 6984.0 17 9946.0 18 8302.0 19 4922.0 20 4331.0 21 4471.0 22 4729.0 23 5143.0 24 5623.0 25 6391.0 26 7235.0 27 8503.0 28 9995.0 29 11993.0 30 14988.0 31 18625.0 32 23766.0 33 31121.0 34 42960.0 35 61398.0 36 86140.0 37 118155.0 38 92885.0 39 10968.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.63980003833028 19.344552187966546 15.521187143572673 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32.490774670317116 19.932824800774704 20-21 21.53581366919204 25.9155688526436 32.23181971722241 20.31679776094195 22-23 20.601308808521008 26.2647968789332 32.727129427603366 20.40676488494243 24-25 20.766881083246073 26.299327475079902 32.72394307746503 20.209848364208998 26-27 20.27228085074898 26.275150351208197 33.37105182311396 20.081516974928864 28-29 19.655733498702226 27.390670331164074 32.97492682667876 19.97866934345494 30-31 20.01682735233033 26.493507955991486 32.8299069991168 20.659757692561378 32-33 19.83635870776146 26.71678972206559 32.83194190508902 20.614909665083932 34-35 20.77533272095197 26.595556427832573 32.43170634007757 20.197404511137886 36-37 20.38379448611073 26.27010690981264 33.065321622095446 20.280776981981184 38-39 20.047879132932543 26.954407871670416 32.74163924512765 20.25607375026939 40-41 20.11694796531289 27.12280823091071 32.613298870328656 20.146944933447745 42-43 19.89668259939052 27.20192897753092 31.731776896826563 21.169611526251998 44-45 20.113165662626376 27.05262565249214 31.66394543597333 21.170263248908157 46-47 20.940576181732343 27.616797887596384 31.096263802728657 20.34636212794262 48-49 20.74974242081803 27.522325539870685 30.75837263041228 20.96955940889901 50-51 20.07374007267837 28.04141605390875 31.263120472682875 20.62172340073 52-53 20.638368567178418 28.597943170490943 29.925316637985617 20.838371624345022 54-55 20.14360427756738 27.956992706388927 30.359646039904554 21.53975697613914 56-57 20.881206335319018 27.936550313292074 29.851926070789858 21.33031728059905 58-59 21.39907701734593 27.634155343818676 29.945391399165473 21.02137623966992 60-61 20.800218709293535 27.70596465271859 31.023109209912676 20.470707428075197 62-63 20.43552907212847 27.984914096393776 30.848662493114443 20.73089433836331 64-65 20.19033680850123 28.468098280374882 30.45453266161943 20.887032249504458 66-67 20.454711760444066 27.891582589592645 29.964031871958934 21.68967377800436 68-69 20.22364833892253 27.907388195935567 30.311547524266768 21.55741594087513 70-71 20.90405248268242 27.815483935300524 29.97159707736178 21.30886650465528 72-73 20.621715160599297 27.51127379397745 30.59363521154848 21.273375833874773 74-75 20.214212593011354 28.695303936934422 29.842199350076516 21.248284119977708 76-77 20.217496021365207 27.963027445543563 29.93831206167668 21.881164471414543 78-79 20.408924803129345 28.062271604302847 29.967365150033455 21.561438442534357 80-81 20.772649671622684 28.08749866308978 29.617808528723266 21.52204313656427 82-83 20.525173087593178 27.88989940414445 30.17264532522777 21.412282183034602 84-85 20.485236969930394 28.036691080751414 29.844308950607108 21.633762998711084 86-87 20.292339495903725 28.242143747924526 29.83972707047008 21.625789685701672 88-89 20.24131359509814 28.040141201823722 29.283054977042646 22.435490226035494 90-91 20.36116277816779 27.720562041077923 29.663404203647815 22.254870977106467 92-93 20.921572647923227 28.046894788261827 29.642620222171296 21.38891234164365 94-95 20.395300270754714 27.821956462127712 29.56718649105271 22.215556776064865 96-97 19.898245961078718 27.98670298097359 30.06970963832848 22.045341419619213 98-99 20.418292013145905 28.35842619017177 28.92477450202279 22.298507294659537 100-101 20.595447317094347 27.34849715155832 29.667046673280606 22.389008858066724 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1051.0 1 667.0 2 413.0 3 513.0 4 399.0 5 364.0 6 663.5 7 1424.5 8 2375.5 9 2677.5 10 2158.0 11 1690.5 12 1682.0 13 1786.5 14 1803.5 15 1832.0 16 1798.0 17 1799.5 18 1894.5 19 1935.0 20 2082.0 21 2356.5 22 2671.0 23 3072.5 24 3606.0 25 4381.0 26 5432.5 27 6485.0 28 7300.5 29 8223.0 30 9370.0 31 10375.5 32 11442.0 33 12619.0 34 14046.5 35 15296.5 36 16818.5 37 18316.5 38 18687.0 39 18776.5 40 18845.0 41 19434.5 42 19876.5 43 20352.0 44 21516.5 45 22449.0 46 22634.5 47 25329.5 48 31641.5 49 30220.5 50 22693.0 51 18614.5 52 16791.0 53 15864.5 54 14841.0 55 13504.0 56 11916.5 57 10388.5 58 9258.0 59 8212.5 60 6872.5 61 5428.5 62 4218.0 63 3181.0 64 2257.5 65 1667.5 66 1261.0 67 954.5 68 690.0 69 472.5 70 342.0 71 268.5 72 212.5 73 189.5 74 157.0 75 132.5 76 102.5 77 69.0 78 53.5 79 41.0 80 32.5 81 27.5 82 20.0 83 13.5 84 10.5 85 8.5 86 7.5 87 5.0 88 1.5 89 0.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.5 95 0.5 96 1.0 97 1.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30970638101109743 2 0.27358199753390877 3 0.2705314940402795 4 0.2750269728729963 5 0.2830546136457049 6 0.19234227291409783 7 0.19218172009864365 8 0.19426890669954788 9 0.1920211672831895 10-11 0.2003699136868064 12-13 0.19105785039046444 14-15 0.19009453349773942 16-17 0.19667719893136046 18-19 0.19105785039046444 20-21 0.18592016029593095 22-23 0.1826288275791204 24-25 0.18640181874229347 26-27 0.18254855117139335 28-29 0.19386752466091245 30-31 0.19924604397862722 32-33 0.1998079788327168 34-35 0.19764051582408548 36-37 0.1780530723386765 38-39 0.17243372379778052 40-41 0.17957832408549115 42-43 0.18808762330456225 44-45 0.19153950883682697 46-47 0.19049591553637485 48-49 0.19178033806000824 50-51 0.19346614262227702 52-53 0.21795044697903826 54-55 0.18294993321002875 56-57 0.1708281956432388 58-59 0.17010570797369504 60-61 0.1634427661323469 62-63 0.17267455302096177 64-65 0.16946349671187835 66-67 0.16817907418824496 68-69 0.17869528360049322 70-71 0.17355759350595973 72-73 0.16890156185778873 74-75 0.17379842272914098 76-77 0.1749222924373202 78-79 0.17981915330867243 80-81 0.1731562114673243 82-83 0.16906211467324292 84-85 0.16175696157007807 86-87 0.16095419749280723 88-89 0.16745658651870118 90-91 0.1731562114673243 92-93 0.16544967632552404 94-95 0.1713901304973284 96-97 0.16930294389642417 98-99 0.1697846023427867 100-101 0.1491535655569256 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 622848.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.41122543555109 #Duplication Level Percentage of deduplicated Percentage of total 1 81.2958216634885 49.11180209481361 2 11.170758735710452 13.496784485383115 3 3.2023203381112064 5.803682875874588 4 1.3928459664741395 3.36574126710669 5 0.7260259941504141 2.1930060002345377 6 0.4626842893704149 1.6770794946386332 7 0.3132124679759574 1.324508430848462 8 0.22957224093328776 1.1094992320612396 9 0.1770620845121642 0.9626883753197656 >10 0.9532030968034131 10.285561234901792 >50 0.05002493532485628 2.011673216505907 >100 0.02011582223038376 2.2711849713579255 >500 0.003176182457421243 1.3787966775551188 >1k 0.0029115005859694726 3.9670585144546555 >5k 2.6468187145177024E-4 1.0409331289439665 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6510 1.0451988286066585 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4735 0.7602175811755035 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3590 0.5763846074804768 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3429 0.550535604192355 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2925 0.4696169852034525 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2401 0.3854873099054665 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1868 0.29991265926839294 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1395 0.22397117755856966 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1177 0.1889706637895602 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1154 0.18527794903411426 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1123 0.18030081175503493 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1013 0.16264000205507603 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 928 0.14899301274147145 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 908 0.14578195643238798 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 861 0.13823597410604194 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 829 0.13309828401150844 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 800 0.12844225236333745 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 767 0.12314400945334977 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 645 0.10355656596794081 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 641 0.10291435470612413 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.0138203863542953E-4 0.0 24-25 0.0 0.0 0.0 0.0011238697081792026 0.0 26-27 0.0 0.0 0.0 0.0015252517468146323 0.0 28-29 0.0 0.0 0.0 0.0028096742704480067 0.0 30-31 0.0 0.0 0.0 0.005217966502260584 0.0 32-33 0.0 0.0 0.0 0.007545982326346075 0.0 34-35 0.0 0.0 0.0 0.01035565659679408 0.0 36-37 0.0 0.0 0.0 0.01332588368269626 0.0 38-39 0.0 0.0 0.0 0.018945232223592273 0.0 40-41 0.0 0.0 0.0 0.025929279695848746 0.0 42-43 0.0 0.0 0.0 0.036445489108097 0.0 44-45 0.0 0.0 0.0 0.05442740443896424 0.0 46-47 0.0 0.0 0.0 0.07658369297163994 0.0 48-49 0.0 0.0 0.0 0.11343056411837238 0.0 50-51 0.0 0.0 0.0 0.15565595458281956 0.0 52-53 0.0 0.0 0.0 0.20109240135635018 0.0 54-55 0.0 0.0 0.0 0.2622630240443896 0.0 56-57 0.0 0.0 0.0 0.33411040896013156 0.0 58-59 0.0 0.0 0.0 0.41727676736539254 0.0 60-61 0.0 0.0 0.0 0.520512227702425 0.0 62-63 0.0 0.0 0.0 0.6451012124948623 0.0 64-65 0.0 0.0 0.0 0.7647130600082204 0.0 66-67 0.0 0.0 0.0 0.9023870992601726 0.0 68-69 0.0 0.0 0.0 1.083892057131114 0.0 70-71 0.0 0.0 0.0 1.2698924938347718 0.0 72-73 0.0 0.0 0.0 1.4917764847924373 0.0 74-75 0.0 0.0 0.0 1.7363786991368682 8.02764077270859E-5 76-77 0.0 0.0 0.0 1.9835497585285653 1.605528154541718E-4 78-79 0.0 0.0 0.0 2.2478999691738597 1.605528154541718E-4 80-81 0.0 0.0 0.0 2.5762304767776407 1.605528154541718E-4 82-83 0.0 0.0 0.0 2.9508001952322234 1.605528154541718E-4 84-85 0.0 0.0 0.0 3.3458403976572133 1.605528154541718E-4 86-87 0.0 0.0 0.0 3.7481857531853677 1.605528154541718E-4 88-89 0.0 0.0 0.0 4.248468326140568 1.605528154541718E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 4515 0.0 26.323637 2 GTACATG 4620 0.0 26.153822 1 ACATGGG 4535 0.0 25.05038 3 GAGTACT 4250 0.0 24.695213 12-13 TAGCCCT 385 0.0 24.692383 4 CATGGGT 475 0.0 24.016592 4 AGTACTT 4355 0.0 23.718134 12-13 GTATCAA 8960 0.0 23.679377 1 GTAGCAC 165 2.4123674E-6 23.046225 3 GTACTTT 4595 0.0 22.996084 14-15 CATGGGA 3295 0.0 22.071394 4 ATGGGCG 110 0.0028643855 21.605835 5 AGAGTAC 7110 0.0 21.541182 10-11 CATGGGG 1230 0.0 20.868074 4 TAGACAG 230 8.938332E-8 20.66645 5 ACTTTTT 5205 0.0 20.483541 16-17 TATCAAC 10285 0.0 20.476847 2 TAAGGTG 210 8.5377906E-7 20.371216 5 TACTTTT 5130 0.0 20.273848 14-15 TCAACGC 10390 0.0 20.266647 4 >>END_MODULE