FastQCFastQC Report
Thu 26 May 2016
SRR937563_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937563_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622848
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66331.0649468249075216No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT61550.9882025791204275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57000.9151510480887793No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40440.6492755856966708No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19550.31388075421290584No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17680.28385737772297576No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14630.23488876900945335No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13600.21835182901767367No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12940.2077553431976983No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12820.20582870941224826No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG12300.19747996300863133No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT12100.19426890669954788No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA11460.18399352651048087No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10690.17163095972050965No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC10160.16312166050143856No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9350.15011688244965066No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA9240.14835080147965474No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC6590.10580430538429922No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA6410.10291435470612413No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGT856.405788E-427.9397356
TAGCGTG700.00839078127.1414597
GGGCGCG908.9456176E-426.387537
GTACATG46800.025.7910081
GTATTAC1501.054841E-625.3442361
TACATGG47900.024.8951072
ACATGGG48050.023.8286533
GCGACAG1000.001653293723.7487757
CGGGAGT1050.002192685822.623334
GAGTACT47800.022.45700312-13
AGTACTT49650.022.33771912-13
CATGGGG13900.021.5328524
GTACTTT51050.020.84123614-15
CATGGGA33150.020.7807064
ATGGGAG10100.020.22665
ATGGGGG7050.020.2165935
TACTTTT54800.020.1517914-15
CTACGTC1908.227811E-620.0037863
AGAGTAC78000.020.00377710-11
ATGGGAT12850.019.9648695
Produced by FastQC (version 0.11.5)