##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937563_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 622848 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02912267519523 31.0 31.0 34.0 30.0 34.0 2 32.31724433569667 33.0 31.0 34.0 31.0 34.0 3 32.39027820591862 34.0 31.0 34.0 31.0 34.0 4 36.049842016029594 37.0 35.0 37.0 35.0 37.0 5 35.97253101880395 37.0 35.0 37.0 35.0 37.0 6 36.049129161528974 37.0 35.0 37.0 35.0 37.0 7 35.9917283189478 37.0 35.0 37.0 35.0 37.0 8 35.97753063347719 37.0 35.0 37.0 35.0 37.0 9 37.62933653154542 39.0 37.0 39.0 35.0 39.0 10-11 37.55050108533703 39.0 37.0 39.0 35.0 39.0 12-13 37.43595467915125 39.0 37.0 39.0 34.5 39.0 14-15 38.516587514128645 40.0 38.0 41.0 34.0 41.0 16-17 38.50933213239827 40.0 38.0 41.0 34.0 41.0 18-19 38.570363074136864 40.0 38.0 41.0 34.0 41.0 20-21 38.5146287697801 40.0 38.0 41.0 34.0 41.0 22-23 38.447167687782574 40.0 38.0 41.0 34.0 41.0 24-25 38.4330711505857 40.0 38.0 41.0 34.0 41.0 26-27 38.25068074393752 40.0 38.0 41.0 34.0 41.0 28-29 38.08896311780723 40.0 37.0 41.0 34.0 41.0 30-31 38.078473399609535 40.0 37.0 41.0 34.0 41.0 32-33 37.90757134967119 40.0 37.0 41.0 33.5 41.0 34-35 37.705143951654335 40.0 36.0 41.0 33.0 41.0 36-37 37.508453105733665 40.0 36.0 41.0 33.0 41.0 38-39 37.341236545674064 40.0 36.0 41.0 33.0 41.0 40-41 37.13292568973489 40.0 36.0 41.0 32.5 41.0 42-43 36.83420674064941 39.0 35.0 41.0 31.5 41.0 44-45 36.398455160809704 39.0 35.0 41.0 30.5 41.0 46-47 35.84890535090423 39.0 35.0 41.0 29.0 41.0 48-49 35.357706053483355 39.0 35.0 40.0 27.0 41.0 50-51 35.0048775945335 39.0 35.0 40.0 25.5 41.0 52-53 34.78174129161529 38.5 35.0 40.0 24.0 41.0 54-55 34.55500780286683 38.0 34.0 40.0 24.0 41.0 56-57 34.24322868500822 38.0 34.0 40.0 23.0 41.0 58-59 33.975764552507194 38.0 34.0 40.0 22.0 41.0 60-61 33.590057285244555 37.5 33.0 40.0 21.5 41.0 62-63 33.232921194256065 37.0 33.0 40.0 19.5 41.0 64-65 32.81991914560214 36.5 32.5 39.0 18.0 40.5 66-67 32.3743995324702 36.0 32.0 39.0 14.0 40.0 68-69 31.896950780928893 35.0 31.0 38.5 9.5 40.0 70-71 31.443290337289355 35.0 31.0 38.0 7.5 40.0 72-73 30.94029763280929 35.0 30.5 37.0 7.0 39.5 74-75 30.454687981658445 34.5 30.0 37.0 3.5 39.0 76-77 29.278675535604194 33.5 28.5 35.5 2.0 38.0 78-79 29.867965378390878 34.0 30.0 36.0 2.0 38.0 80-81 29.63652769214961 34.0 29.5 35.5 2.0 37.0 82-83 29.23980730451089 34.0 29.0 35.0 2.0 37.0 84-85 28.831342478421703 34.0 29.0 35.0 2.0 36.5 86-87 28.46744711390259 34.0 29.0 35.0 2.0 36.0 88-89 27.97676559931155 33.5 27.5 35.0 2.0 36.0 90-91 27.365512131370735 33.0 26.0 35.0 2.0 35.0 92-93 26.867942900996713 33.0 25.5 35.0 2.0 35.0 94-95 26.316012895602135 32.0 25.0 34.0 2.0 35.0 96-97 25.6510368500822 32.0 23.5 34.0 2.0 35.0 98-99 24.70621002234895 32.0 13.0 34.0 2.0 35.0 100-101 21.990871769677355 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34475176088243487 1101 2 0.2999759280701326 1101 3 0.31114732135184653 1101 4 0.23706215719265344 1101 5 0.05148800163758693 1101 6 0.13356397267382647 1101 7 0.20365125624267932 1101 8 0.20601920877780344 1101 9 0.22863057103630524 1101 10-11 0.37991878969473447 1101 12-13 0.24941205860440618 1101 14-15 0.40337145452904366 1101 16-17 0.2176740280599745 1101 18-19 0.34393411884639136 1101 20-21 0.3545215212427806 1101 22-23 0.3034772010004616 1101 24-25 0.2315168370333609 1101 26-27 0.18835456329659905 1101 28-29 0.1549505794857069 1101 30-31 0.1925045116218982 1101 32-33 0.45942767885317437 1101 34-35 0.38950661141257825 1101 36-37 0.26373909762425285 1101 38-39 0.5510588621781523 1101 40-41 0.36756994736968096 1101 42-43 0.448504264137199 1101 44-45 0.528938089674007 1101 46-47 0.5438775670974607 1101 48-49 0.7157788156318503 1101 50-51 0.7812645942428347 1101 52-53 0.574919607297339 1101 54-55 0.43395935896582927 1101 56-57 0.7047184146576484 1101 58-59 0.577984642364683 1101 60-61 0.4648339388524647 1101 62-63 0.3205884084051718 1101 64-65 0.35990974737265446 1101 66-67 0.3214486960509362 1101 68-69 0.7092203952643672 1101 70-71 0.6027019906868745 1101 72-73 0.6082486234791808 1101 74-75 0.5274280259637045 1101 76-77 0.6294481719555236 1101 78-79 0.7277180705880575 1101 80-81 0.5977246116285322 1101 82-83 0.6797616093464356 1101 84-85 0.7425425065849645 1101 86-87 0.9142057610559782 1101 88-89 0.6109368337422474 1101 90-91 0.6695945931168907 1101 92-93 0.4735989657990949 1101 94-95 0.5784436090279925 1101 96-97 0.7004089903969763 1101 98-99 0.053426188810824726 1101 100-101 -0.2665277190755937 1102 1 0.2607809265530001 1102 2 0.2665143701046304 1102 3 0.13822879832574841 1102 4 0.06877457180011959 1102 5 -0.009902257636966283 1102 6 5.001283979382265E-4 1102 7 0.01139580669581619 1102 8 0.06044440745354507 1102 9 0.09297144571664262 1102 10-11 0.08639395251103821 1102 12-13 0.20113765789157156 1102 14-15 0.18042178088608551 1102 16-17 0.14189200269033364 1102 18-19 0.26805429502847744 1102 20-21 0.05346348691914926 1102 22-23 0.08307934242425574 1102 24-25 0.1315849337306645 1102 26-27 -0.00979233721702144 1102 28-29 0.041623005394335166 1102 30-31 0.23029998971141907 1102 32-33 0.3068756826781822 1102 34-35 0.22064038520115048 1102 36-37 0.31133253136399475 1102 38-39 0.3867447915169606 1102 40-41 0.42630153771734314 1102 42-43 0.25611117232980973 1102 44-45 0.3497043224053371 1102 46-47 0.4675694222978066 1102 48-49 0.563128115467407 1102 50-51 0.569179603769129 1102 52-53 0.4336397878260314 1102 54-55 0.379170494949129 1102 56-57 0.3223943975320509 1102 58-59 0.4841560866666512 1102 60-61 0.2508545562142217 1102 62-63 0.08241296523331698 1102 64-65 0.3153163715875493 1102 66-67 0.1390962449320483 1102 68-69 0.20136935316757132 1102 70-71 0.08346829586149696 1102 72-73 0.045020115593729315 1102 74-75 -0.09192320810782562 1102 76-77 0.10669282745143605 1102 78-79 0.2545112232478104 1102 80-81 0.3102397341663625 1102 82-83 0.2982236019717064 1102 84-85 0.38535902945168843 1102 86-87 -0.07121412028190122 1102 88-89 0.21973126864832793 1102 90-91 0.24389696418593587 1102 92-93 0.28433361325379636 1102 94-95 0.06078193370435159 1102 96-97 0.22378553615578767 1102 98-99 0.20311124158576277 1102 100-101 0.4223201789822042 1103 1 0.27508527633483837 1103 2 0.1784391598126973 1103 3 0.17063849649496632 1103 4 0.10458042848672733 1103 5 0.11944415053180535 1103 6 0.010328746093946961 1103 7 0.045379882985677966 1103 8 0.16124958940667256 1103 9 0.19321434976954777 1103 10-11 0.130802661071165 1103 12-13 0.26098651759198077 1103 14-15 0.29021216368488467 1103 16-17 0.22026229642430906 1103 18-19 0.09119540429031758 1103 20-21 0.13169883395706705 1103 22-23 -0.022314027194738628 1103 24-25 -0.0042501614067447235 1103 26-27 0.0092463924847479 1103 28-29 0.1154950759264679 1103 30-31 0.17064230449707907 1103 32-33 0.23442055245461546 1103 34-35 0.29514220698154503 1103 36-37 0.20867183234010866 1103 38-39 0.15409668751803451 1103 40-41 0.03352793329109005 1103 42-43 0.13803281328163308 1103 44-45 0.2752699739729607 1103 46-47 0.27898141456086734 1103 48-49 0.3268476962330027 1103 50-51 0.1836368931398269 1103 52-53 0.21612820490128115 1103 54-55 0.15086317633998902 1103 56-57 0.2926611421679226 1103 58-59 0.14547361624246946 1103 60-61 0.21175375103226912 1103 62-63 0.3516990972111813 1103 64-65 0.12118430375434741 1103 66-67 0.20481528591086118 1103 68-69 0.33381953534560793 1103 70-71 0.2574933059825 1103 72-73 0.2670861495103303 1103 74-75 0.28131686206445394 1103 76-77 0.36886052121239743 1103 78-79 0.3679339212820878 1103 80-81 0.46445007108328085 1103 82-83 0.3950253652752025 1103 84-85 0.41487861921373437 1103 86-87 0.15749803841541166 1103 88-89 0.25287192808784553 1103 90-91 0.385770923971819 1103 92-93 0.3361643900969469 1103 94-95 -0.13157333596412712 1103 96-97 -0.2176700888723282 1103 98-99 -0.09075768019013708 1103 100-101 -0.07688138639947084 1104 1 0.30869266808176477 1104 2 0.24025890767895675 1104 3 0.2171814453635008 1104 4 0.25759898615644516 1104 5 0.2210885010133694 1104 6 0.1311780661680686 1104 7 0.06304229522173443 1104 8 0.17336558136762648 1104 9 0.22296404308512763 1104 10-11 0.19080448812724882 1104 12-13 0.14421022755987423 1104 14-15 0.2716818998057775 1104 16-17 0.12262963910173141 1104 18-19 0.11130476664774847 1104 20-21 -0.03617760484959831 1104 22-23 -0.030799397695616904 1104 24-25 -0.05472258591018431 1104 26-27 -0.11284532573100847 1104 28-29 0.1055089093511512 1104 30-31 0.03280136278016954 1104 32-33 -0.013179478866341299 1104 34-35 0.04913693461968904 1104 36-37 -0.04212163201924568 1104 38-39 -0.23305127792450264 1104 40-41 -0.20167682749507065 1104 42-43 -0.08908069910319938 1104 44-45 0.33785056019696214 1104 46-47 0.30103528661474144 1104 48-49 0.4141835874448674 1104 50-51 0.36701851295556054 1104 52-53 0.47214182951955763 1104 54-55 0.5069029017595525 1104 56-57 0.6015106188468593 1104 58-59 0.5351076830083201 1104 60-61 0.5254109953096702 1104 62-63 0.5432267771109522 1104 64-65 0.5669807697058502 1104 66-67 0.43003518516177053 1104 68-69 0.5887251809489271 1104 70-71 0.4500872036151584 1104 72-73 0.6536155259621879 1104 74-75 0.5373565874840267 1104 76-77 0.37126048989144067 1104 78-79 0.5863114954736162 1104 80-81 0.5080762554071434 1104 82-83 0.6335866891078474 1104 84-85 0.7977512734042165 1104 86-87 0.6246475699060277 1104 88-89 0.7093866880909765 1104 90-91 0.6792457236481688 1104 92-93 0.4225932784638182 1104 94-95 0.6213760545821607 1104 96-97 0.7370541561619923 1104 98-99 0.6450304335049566 1104 100-101 0.33344800571286726 1105 1 0.32743436530266123 1105 2 0.2623642267713606 1105 3 0.24422326480129897 1105 4 0.09099608483331423 1105 5 0.09422735004891791 1105 6 0.08486140038703383 1105 7 0.04832667094130727 1105 8 0.036362198261898016 1105 9 0.07018936501155082 1105 10-11 0.2964840318507882 1105 12-13 0.17770828803067928 1105 14-15 0.16824122814416143 1105 16-17 0.15469209371320858 1105 18-19 0.10853185983371816 1105 20-21 0.09666951773927224 1105 22-23 0.2353615743716233 1105 24-25 0.11818334249551299 1105 26-27 -0.127111935717366 1105 28-29 -0.005803239718048303 1105 30-31 0.298457525877744 1105 32-33 0.3319451292424844 1105 34-35 0.5106793474294378 1105 36-37 0.3862814003770936 1105 38-39 0.5294883540077677 1105 40-41 0.5957858432100736 1105 42-43 0.5064760963340404 1105 44-45 0.8011305786180642 1105 46-47 0.9416591242256587 1105 48-49 0.8118862638180318 1105 50-51 0.7286326617767784 1105 52-53 0.7506130554082873 1105 54-55 0.6604436495853321 1105 56-57 0.9487961635977342 1105 58-59 1.0172011346617893 1105 60-61 1.0580491092725097 1105 62-63 0.930136283460925 1105 64-65 0.9097602301801899 1105 66-67 0.7422878730481273 1105 68-69 0.7227333641680147 1105 70-71 0.6431562650247251 1105 72-73 0.7628464450687673 1105 74-75 0.6890321990113826 1105 76-77 0.39070360589122544 1105 78-79 0.6302511309484018 1105 80-81 0.7527704474471015 1105 82-83 0.7324318278860282 1105 84-85 0.8217651246538402 1105 86-87 0.580196040423413 1105 88-89 0.7036690622872719 1105 90-91 0.7973028290558233 1105 92-93 0.95904757111132 1105 94-95 0.7009321769407713 1105 96-97 0.8305917157205975 1105 98-99 0.765228106069916 1105 100-101 0.6254968216258625 1106 1 0.13324778346879995 1106 2 0.0355176638884771 1106 3 0.23424111963879568 1106 4 0.08014190258730736 1106 5 0.12665348875152915 1106 6 -0.10540832214440599 1106 7 0.08048973700420703 1106 8 -0.026336780870110488 1106 9 0.03617748289732248 1106 10-11 -0.05804897767584549 1106 12-13 -0.23940921531253423 1106 14-15 -0.13544568618428343 1106 16-17 -0.012490568825498372 1106 18-19 -0.003125424720757053 1106 20-21 0.21253961831583723 1106 22-23 0.2044822053562214 1106 24-25 0.04943491057895244 1106 26-27 0.11158507524155681 1106 28-29 0.023418528081371903 1106 30-31 -0.07461966889100324 1106 32-33 0.11628037147635695 1106 34-35 -0.2822240245284817 1106 36-37 0.18882955119565992 1106 38-39 0.19709607372823257 1106 40-41 0.1343698303682288 1106 42-43 -0.195413885713684 1106 44-45 0.18189046880959125 1106 46-47 0.3005511012409059 1106 48-49 0.37960310996682267 1106 50-51 0.7809037337734281 1106 52-53 0.6020135518361016 1106 54-55 0.5542451223049412 1106 56-57 0.6534484083624648 1106 58-59 0.5209353046310738 1106 60-61 0.3394722835362103 1106 62-63 0.4026086547441068 1106 64-65 -0.07554789364907322 1106 66-67 -0.18981746318550208 1106 68-69 0.4208197542998704 1106 70-71 0.22513384051804763 1106 72-73 0.43301668716866715 1106 74-75 0.37570050582228376 1106 76-77 0.14503273412208983 1106 78-79 0.30842681962629115 1106 80-81 0.3378756643237004 1106 82-83 0.45910179108021865 1106 84-85 0.00924700796163691 1106 86-87 0.5249032868567092 1106 88-89 0.5136563227511068 1106 90-91 0.2089895874893699 1106 92-93 0.6631286072277831 1106 94-95 0.6278004189578574 1106 96-97 0.6801141658037828 1106 98-99 0.3220725010393828 1106 100-101 -0.20035274466966158 1107 1 0.15721505806756753 1107 2 0.1368262066852779 1107 3 0.08302219900912888 1107 4 -0.02408897358029094 1107 5 0.1909684855274989 1107 6 0.19041581727574197 1107 7 0.07333179494212771 1107 8 -0.1257611876778455 1107 9 0.19183314447455757 1107 10-11 0.011538346259477805 1107 12-13 0.24350045855742763 1107 14-15 0.19765299285003834 1107 16-17 0.3748191689335343 1107 18-19 0.23360871984601062 1107 20-21 0.10681503936255865 1107 22-23 0.1145376350397953 1107 24-25 0.08098309654346991 1107 26-27 0.21983415979401144 1107 28-29 0.04835744894766236 1107 30-31 0.18091294707061678 1107 32-33 0.03962088247060791 1107 34-35 0.19985745769793084 1107 36-37 0.42045848584542256 1107 38-39 0.27431311317972984 1107 40-41 0.16439621418772532 1107 42-43 0.41680608198684155 1107 44-45 0.4121676763839943 1107 46-47 0.2490479334078728 1107 48-49 0.08304838720397356 1107 50-51 0.12610140537177728 1107 52-53 0.23882785352265756 1107 54-55 0.037108949851777595 1107 56-57 -0.05586667714833027 1107 58-59 0.052198060261304136 1107 60-61 0.10647091701999756 1107 62-63 -0.09492008054809986 1107 64-65 0.11733350339191873 1107 66-67 0.09558772957610273 1107 68-69 -0.06333960160365848 1107 70-71 0.05620197111204206 1107 72-73 -0.03511420260493736 1107 74-75 -0.10595652818681245 1107 76-77 0.023363987116887586 1107 78-79 0.08300784338947764 1107 80-81 -0.04191083970190235 1107 82-83 0.046328118109435934 1107 84-85 0.2921483999151633 1107 86-87 0.5255229516757218 1107 88-89 0.06506484011059399 1107 90-91 0.24314214039011262 1107 92-93 0.13879751987131428 1107 94-95 0.1878147348419823 1107 96-97 0.2906245093258413 1107 98-99 -0.2136610105523431 1107 100-101 0.04366652853301645 1108 1 0.13987217765027182 1108 2 -0.05065282920527636 1108 3 0.06869130492836462 1108 4 -0.043143209870621035 1108 5 0.041957306950934026 1108 6 0.049044131316392736 1108 7 -0.022155017349511752 1108 8 -0.03361494205035598 1108 9 0.14786156667289418 1108 10-11 -0.06715835356185806 1108 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pass #Quality Count 2 2.0 3 3.0 4 6.0 5 15.0 6 32.0 7 92.0 8 194.0 9 365.0 10 652.0 11 1146.0 12 1838.0 13 2590.0 14 3527.0 15 4926.0 16 7137.0 17 10494.0 18 9247.0 19 4992.0 20 4011.0 21 3881.0 22 3973.0 23 4234.0 24 4963.0 25 5702.0 26 6815.0 27 8261.0 28 10119.0 29 12343.0 30 15466.0 31 19140.0 32 24337.0 33 32191.0 34 44065.0 35 63021.0 36 92324.0 37 128335.0 38 86578.0 39 5830.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.11177163925528 20.31059810448763 16.123417060895985 19.4542131953611 2 15.47488311755035 22.58929947595561 39.746615546650226 22.189201859843813 3 20.421194256062474 26.733809854089603 30.641344276613236 22.20365161323469 4 12.196394625976161 18.16045006165228 38.57923602548294 31.063919286888613 5 12.049344091685832 39.95580167285529 33.609358738805874 14.385495496653006 6 29.004668875873406 38.59946568023017 16.947152435265107 15.448713008631321 7 26.553187936703658 35.64272503082614 20.436928431976984 17.36715860049322 8 26.368231093300455 33.99882475339088 19.34003159679408 20.29291255651459 9 26.458301222770242 17.90099671187834 21.16342992190711 34.47727214344431 10-11 24.899654490341142 27.989493423756677 26.22533908754624 20.88551299835594 12-13 26.916574210402093 26.012215466180272 27.321026882695882 19.750183440721752 14-15 22.782283960131526 26.788156982120835 28.378834001233045 22.05072505651459 16-17 19.918182285244555 30.3252960593917 28.4972417026305 21.25927995273325 18-19 19.74004893649815 28.312044030004106 32.53241561344019 19.415491420057542 20-21 21.551245247636665 25.936905954582816 32.74755638614879 19.764292411631732 22-23 20.768426148099568 26.490078984401404 32.82111345608735 19.92038141141168 24-25 21.109982780696722 26.69762662610029 32.88959175400078 19.30279883920221 26-27 20.51454544578793 26.540641993703044 33.673682368373306 19.271130192135715 28-29 19.56480204481707 28.098332966734795 33.025497597307826 19.3113673911403 30-31 20.268508528565558 26.65666422626387 32.97481889642417 20.100008348746403 32-33 20.04887227702425 27.160880343197697 32.84918631833128 19.941061061446774 34-35 21.03996480682285 26.650001284422526 32.81916294184135 19.490870966913278 36-37 20.736921367653103 26.48037723489519 33.2250404593095 19.55766093814221 38-39 20.198266671804358 27.30577925914509 33.0519645242499 19.443989544800658 40-41 20.18950261784308 27.544349199876052 32.503399744075935 19.762748438204937 42-43 20.089392166985274 27.697922880589115 31.740401421732074 20.472283530693534 44-45 20.779307310933003 27.120421033703245 31.62986796136457 20.470403693999177 46-47 21.414855630908345 28.107901125154132 30.948240983353887 19.529002260583642 48-49 21.134851520756268 28.24244438450473 30.523097128031235 20.099606966707768 50-51 20.403854551993426 28.685971537196874 30.923596126181668 19.986577784628032 52-53 21.101617087957255 29.21459168207974 29.434870144882865 20.24892108508015 54-55 20.408109201602958 28.69913686806412 29.843637613029184 21.04911631730374 56-57 21.389729115289764 28.442172086929716 29.727638203863542 20.440460593916974 58-59 22.00288031750925 27.91066199136868 29.571099208795726 20.515358482326345 60-61 20.865203067200984 28.478216193999177 30.84364082408549 19.812939914714345 62-63 20.580302096177558 28.46400726983148 30.618786606041926 20.336904027949036 64-65 20.421435085285655 29.20431630189067 30.063996352240036 20.310252260583642 66-67 20.915696927661322 28.366471434443074 29.53658035347308 21.181251284422522 68-69 20.60478640053432 28.585545751130294 29.76962276510481 21.04004508323058 70-71 21.437654130702835 28.627209206740652 29.437438989930126 20.497697672626387 72-73 21.20613697081792 27.98893148890259 30.194525791204274 20.610405749075216 74-75 20.596678483353884 29.646077373612822 29.096585362720923 20.66065878031237 76-77 20.78516748869708 28.322158857377723 29.49170584155364 21.400967812371558 78-79 20.831888357994245 28.791053354911632 29.333481041923555 21.04357724517057 80-81 21.10121570591862 28.77652332511303 29.267895216810523 20.85436575215783 82-83 20.942694180310433 28.52567061496285 29.68543411857733 20.84620108614939 84-85 20.542893290176735 28.939083050760377 29.45526035244554 21.062763306617345 86-87 20.459164996917387 29.007558826551584 29.060541255651458 21.47273492087957 88-89 20.396308569667077 28.86715538943691 28.789768932388 21.946767108508013 90-91 20.842409172692918 28.360536096081727 28.964539394294608 21.83251533693075 92-93 21.247275573484256 28.77227206133221 29.08206394123668 20.898388423946855 94-95 20.64998201808467 28.700581843403207 28.931135686395397 21.71830045211673 96-97 19.985293362104397 28.837693947801068 29.463047163995064 21.713965526099464 98-99 20.690302925699513 29.26542424449199 28.214998173695598 21.8292746561129 100-101 20.35723519294972 28.54549425350113 28.68375708777683 22.41351346577232 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 869.0 1 574.0 2 408.5 3 554.5 4 486.5 5 440.5 6 735.5 7 1502.0 8 2299.0 9 2665.0 10 2352.0 11 1887.5 12 1859.0 13 1935.0 14 2009.5 15 2081.0 16 2106.0 17 2069.5 18 2097.0 19 2192.0 20 2318.5 21 2512.0 22 2853.0 23 3317.5 24 3786.5 25 4443.5 26 5425.0 27 6394.5 28 7224.5 29 8298.0 30 9532.0 31 10407.5 32 11304.0 33 12712.0 34 14051.0 35 15073.5 36 16762.0 37 18170.5 38 18669.0 39 18696.5 40 18863.0 41 19557.0 42 19743.0 43 20192.0 44 21343.5 45 22143.5 46 21886.0 47 25017.0 48 32558.5 49 30639.0 50 22282.5 51 18488.0 52 16669.5 53 15611.5 54 14642.5 55 13471.0 56 11805.0 57 10304.5 58 9261.5 59 8066.0 60 6670.0 61 5328.5 62 4143.0 63 3082.5 64 2144.5 65 1565.5 66 1173.5 67 883.0 68 659.5 69 464.0 70 320.0 71 248.0 72 210.5 73 178.5 74 138.5 75 118.0 76 94.5 77 61.0 78 40.0 79 34.5 80 27.5 81 20.0 82 18.5 83 15.0 84 11.0 85 6.5 86 3.5 87 2.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018303020961775586 2 0.0 3 0.0 4 0.0 5 0.03211056309083436 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004977137279079326 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.619348540896014E-4 24-25 8.02764077270859E-5 26-27 0.0011238697081792028 28-29 8.02764077270859E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0011238697081792028 42-43 0.013887818536785862 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.83040484997945E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.013887818536785862 92-93 0.0016858045622688037 94-95 0.0 96-97 0.0 98-99 8.83040484997945E-4 100-101 0.01942689066995479 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 622848.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.270725758032924 #Duplication Level Percentage of deduplicated Percentage of total 1 80.2874124325432 46.784057916788086 2 11.577427940310042 13.49250256986389 3 3.4412646083077183 6.01574958754571 4 1.502696873872651 3.5025294973954657 5 0.8336355776783362 2.4288275064516847 6 0.4764401930161216 1.665750949640802 7 0.34322303863061365 1.399989889051826 8 0.2433593990575267 1.134458304249667 9 0.18690796411681695 0.9802136447138969 >10 1.0188770540722238 10.58526999983916 >50 0.05512729076189467 2.1744105164788716 >100 0.027839265499333906 3.1474167886549593 >500 0.0016538177524342676 0.693857957082218 >1k 0.003307635504868535 3.0255098003605876 >5k 8.269088762171338E-4 2.9694550718833437 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6633 1.0649468249075216 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6155 0.9882025791204275 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5700 0.9151510480887793 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4044 0.6492755856966708 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1955 0.31388075421290584 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1768 0.28385737772297576 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1463 0.23488876900945335 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1360 0.21835182901767367 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1294 0.2077553431976983 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1282 0.20582870941224826 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1230 0.19747996300863133 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1210 0.19426890669954788 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1146 0.18399352651048087 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1069 0.17163095972050965 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1016 0.16312166050143856 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 935 0.15011688244965066 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 924 0.14835080147965474 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 659 0.10580430538429922 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 641 0.10291435470612413 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.211056309083436E-4 0.0 24-25 0.0 0.0 0.0 9.633168927250308E-4 8.02764077270859E-5 26-27 0.0 0.0 0.0 0.0013646989313604602 1.605528154541718E-4 28-29 0.0 0.0 0.0 0.0026491214549938347 1.605528154541718E-4 30-31 0.0 0.0 0.0 0.00529824290998767 1.605528154541718E-4 32-33 0.0 0.0 0.0 0.0077065351418002465 1.605528154541718E-4 34-35 0.0 0.0 0.0 0.010435933004521166 1.605528154541718E-4 36-37 0.0 0.0 0.0 0.013406160090423346 1.605528154541718E-4 38-39 0.0 0.0 0.0 0.018864955815865187 1.605528154541718E-4 40-41 0.0 0.0 0.0 0.025527897657213316 1.605528154541718E-4 42-43 0.0 0.0 0.0 0.035883554254007397 1.605528154541718E-4 44-45 0.0 0.0 0.0 0.05354436395396629 1.605528154541718E-4 46-47 0.0 0.0 0.0 0.07570065248664201 1.605528154541718E-4 48-49 0.0 0.0 0.0 0.11302918207973695 1.605528154541718E-4 50-51 0.0 0.0 0.0 0.15509401972872996 1.605528154541718E-4 52-53 0.0 0.0 0.0 0.20117267776407727 1.605528154541718E-4 54-55 0.0 0.0 0.0 0.263547446568023 1.605528154541718E-4 56-57 0.0 0.0 0.0 0.3357159371146733 1.605528154541718E-4 58-59 0.0 0.0 0.0 0.41928367755856966 1.605528154541718E-4 60-61 0.0 0.0 0.0 0.5227599671187834 1.605528154541718E-4 62-63 0.0 0.0 0.0 0.6472686755034935 1.605528154541718E-4 64-65 0.0 0.0 0.0 0.7670410758323059 1.605528154541718E-4 66-67 0.0 0.0 0.0 0.9043137330456228 1.605528154541718E-4 68-69 0.0 0.0 0.0 1.0871031134401972 1.605528154541718E-4 70-71 0.0 0.0 0.0 1.2754315659679407 1.605528154541718E-4 72-73 0.0 0.0 0.0 1.497957768187423 1.605528154541718E-4 74-75 0.0 0.0 0.0 1.7450485511713933 1.605528154541718E-4 76-77 0.0 0.0 0.0 1.9951898376489932 1.605528154541718E-4 78-79 0.0 0.0 0.0 2.261306129264283 1.605528154541718E-4 80-81 0.0 0.0 0.0 2.5917238234689686 1.605528154541718E-4 82-83 0.0 0.0 0.0 2.9674174116317302 1.605528154541718E-4 84-85 0.0 0.0 0.0 3.363260378133991 1.605528154541718E-4 86-87 0.0 0.0 0.0 3.766408497739416 1.605528154541718E-4 88-89 0.0 0.0 0.0 4.267333281956432 1.605528154541718E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAGCGT 85 6.405788E-4 27.939735 6 TAGCGTG 70 0.008390781 27.141459 7 GGGCGCG 90 8.9456176E-4 26.38753 7 GTACATG 4680 0.0 25.791008 1 GTATTAC 150 1.054841E-6 25.344236 1 TACATGG 4790 0.0 24.895107 2 ACATGGG 4805 0.0 23.828653 3 GCGACAG 100 0.0016532937 23.748775 7 CGGGAGT 105 0.0021926858 22.62333 4 GAGTACT 4780 0.0 22.457003 12-13 AGTACTT 4965 0.0 22.337719 12-13 CATGGGG 1390 0.0 21.532852 4 GTACTTT 5105 0.0 20.841236 14-15 CATGGGA 3315 0.0 20.780706 4 ATGGGAG 1010 0.0 20.2266 5 ATGGGGG 705 0.0 20.216593 5 TACTTTT 5480 0.0 20.15179 14-15 CTACGTC 190 8.227811E-6 20.003786 3 AGAGTAC 7800 0.0 20.003777 10-11 ATGGGAT 1285 0.0 19.964869 5 >>END_MODULE