FastQCFastQC Report
Thu 26 May 2016
SRR937562_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937562_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557086
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57951.0402343623785197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43610.7828234778831276No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32230.5785462208707454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31400.5636472645157121No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27490.4934606146986282No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21700.38952693120990295No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16960.3044413250377859No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT13220.23730626869100999No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11050.1983535755700197No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC10910.19584049859447195No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT10090.1811210477376922No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8780.15760582746649532No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC8200.14719450856779745No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7760.13929626664464734No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7270.13050049723023016No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7230.1297824752372165No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG6820.12242274980882664No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT6700.1202686838297857No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA6430.1154220353769436No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA6000.10770329895204689No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC5880.10554923297300596No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC250.00499647557.0674321
CGTATCA550.002568110434.5738562
GATGCCG650.00583347729.2389979
CCGGGAC1004.9239352E-528.5208633
GAGTACT37450.025.36744712-13
GTGTATC950.001217336425.0295731
GTACATG40550.024.7456381
GTGTAGC4000.023.7780971
GTTACGG500.001650904123.75668524-25
TACATGG41400.023.6546972
GTCTAGG1252.2358909E-422.8269731
AGTACTT38600.022.51968612-13
GTACTTT41650.022.46825214-15
ACATGGG42400.022.1978423
GGTCGGG1302.9154538E-421.9490131
GTCGGGA1302.9225697E-421.9411012
ATGGGAT11350.020.9404285
CATGGGA30050.020.5659794
GTATCAA89050.020.4537011
GTCCATA1404.808895E-420.3812241
Produced by FastQC (version 0.11.5)