##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937562_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 557086 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.889316909776948 33.0 31.0 34.0 30.0 34.0 2 32.00460970119515 33.0 31.0 34.0 30.0 34.0 3 32.02873344510542 34.0 31.0 34.0 30.0 34.0 4 35.60450989613812 37.0 35.0 37.0 35.0 37.0 5 35.596116219039786 37.0 35.0 37.0 33.0 37.0 6 35.693786955694456 37.0 35.0 37.0 35.0 37.0 7 35.65451653784155 37.0 35.0 37.0 33.0 37.0 8 35.64648905195966 37.0 35.0 37.0 33.0 37.0 9 37.28175542016852 39.0 37.0 39.0 34.0 39.0 10-11 37.18292687305012 39.0 37.0 39.0 34.0 39.0 12-13 37.06838082450466 39.0 37.0 39.0 33.5 39.0 14-15 38.18158327439569 40.0 38.0 41.0 33.5 41.0 16-17 38.16455179272141 40.0 38.0 41.0 33.5 41.0 18-19 38.19886875635001 40.0 38.0 41.0 33.5 41.0 20-21 38.011233454080696 40.0 38.0 41.0 33.0 41.0 22-23 37.93338730465314 40.0 38.0 41.0 33.0 41.0 24-25 37.78542271749784 40.0 37.5 41.0 33.0 41.0 26-27 37.857212351414326 40.0 38.0 41.0 33.0 41.0 28-29 37.802801542311244 40.0 38.0 41.0 33.0 41.0 30-31 37.55123535683898 40.0 37.0 41.0 33.0 41.0 32-33 37.24708931834582 39.5 36.0 41.0 32.5 41.0 34-35 36.958982993649094 39.0 35.5 41.0 31.5 41.0 36-37 36.94908416294791 39.0 36.0 41.0 31.0 41.0 38-39 36.87037728465623 39.0 36.0 41.0 31.0 41.0 40-41 36.68453075467701 39.5 35.5 41.0 31.0 41.0 42-43 36.3839873915338 39.0 35.0 41.0 30.0 41.0 44-45 36.037975285683004 39.0 35.0 41.0 29.5 41.0 46-47 35.539930100558976 39.0 35.0 41.0 27.5 41.0 48-49 35.07943836319706 39.0 35.0 40.5 24.5 41.0 50-51 34.12032343300675 38.0 34.0 39.5 23.5 40.5 52-53 34.20284300808134 38.0 34.5 39.5 23.0 40.5 54-55 34.518854360009044 39.0 35.0 40.0 22.5 41.0 56-57 34.257193682842505 38.0 34.0 40.0 21.0 41.0 58-59 34.0409864904162 38.0 34.0 40.0 20.0 41.0 60-61 33.72450662913805 38.0 34.0 40.0 18.0 41.0 62-63 33.385303166835996 37.5 33.0 40.0 15.0 41.0 64-65 33.0302574467856 37.0 33.0 40.0 11.5 41.0 66-67 32.607228506909166 36.5 32.5 39.0 8.0 41.0 68-69 32.1357447503617 36.0 32.0 39.0 6.0 40.5 70-71 31.677781347942688 35.5 31.0 39.0 2.0 40.0 72-73 31.21450548030286 35.0 31.0 38.0 2.0 40.0 74-75 30.755132959722555 35.0 31.0 37.0 2.0 39.5 76-77 30.30436144509106 35.0 30.0 37.0 2.0 39.0 78-79 29.788063243377145 34.5 29.5 36.0 2.0 39.0 80-81 29.25784708285615 34.0 29.0 36.0 2.0 37.5 82-83 28.75516527071224 34.0 29.0 35.0 2.0 37.0 84-85 28.28318069382465 34.0 27.5 35.0 2.0 37.0 86-87 27.83083760855595 34.0 27.0 35.0 2.0 36.0 88-89 27.33320259349544 33.5 26.0 35.0 2.0 36.0 90-91 26.808976531451158 33.0 25.0 35.0 2.0 35.5 92-93 26.280021397055393 33.0 24.5 35.0 2.0 35.0 94-95 25.67853527103535 32.0 21.5 35.0 2.0 35.0 96-97 24.958230865611412 32.0 18.5 34.5 2.0 35.0 98-99 24.116228015064102 32.0 2.0 34.0 2.0 35.0 100-101 21.68316651289029 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.016732724849426006 1101 2 0.1392230569011197 1101 3 0.15031148139855688 1101 4 0.11060923813433732 1101 5 0.1108615807053468 1101 6 0.03786180281635154 1101 7 0.14937629528068186 1101 8 -0.01724120329999579 1101 9 0.08239402690873021 1101 10-11 0.08618595800793116 1101 12-13 0.2496849909516996 1101 14-15 0.40151495107966895 1101 16-17 0.5047457273481086 1101 18-19 0.37550676965457797 1101 20-21 0.5279146448577166 1101 22-23 0.519536664675428 1101 24-25 0.4909324733356968 1101 26-27 0.6967202107360393 1101 28-29 0.6267420761024383 1101 30-31 0.8210039870950538 1101 32-33 0.7481073819101525 1101 34-35 0.6813534223166968 1101 36-37 0.6650588083944342 1101 38-39 0.7202923793549516 1101 40-41 0.8395877240729561 1101 42-43 0.751669652084459 1101 44-45 0.9480194065385845 1101 46-47 1.2346487104998118 1101 48-49 1.3351521663554422 1101 50-51 1.480295525399221 1101 52-53 1.2504492457592065 1101 54-55 1.561888622146661 1101 56-57 1.5863105721484985 1101 58-59 1.4312838056493362 1101 60-61 1.6099830917485534 1101 62-63 1.526353953954505 1101 64-65 1.5294181067141892 1101 66-67 1.7271795396899208 1101 68-69 1.7602201136218234 1101 70-71 1.6094647852169928 1101 72-73 1.627182815454038 1101 74-75 1.617113111259421 1101 76-77 1.6285489307386705 1101 78-79 1.7258809283058945 1101 80-81 1.8768250540796387 1101 82-83 2.1641830376729523 1101 84-85 2.0238703379632277 1101 86-87 2.07153444084733 1101 88-89 2.042781271803591 1101 90-91 2.1775599348034795 1101 92-93 2.382571917623334 1101 94-95 2.4250770797972656 1101 96-97 2.4644248163666127 1101 98-99 2.464831750911131 1101 100-101 2.5545885127045516 1102 1 0.06722945212089826 1102 2 0.16855866801443753 1102 3 0.25430788200052135 1102 4 0.13831753519500012 1102 5 0.08413415408531222 1102 6 0.13541531541855534 1102 7 0.1545581872746098 1102 8 0.15391293126715055 1102 9 -0.08675354668672242 1102 10-11 0.10221344996186588 1102 12-13 0.14635442727010428 1102 14-15 0.28430050246159766 1102 16-17 0.39278815818295243 1102 18-19 0.2920504683808218 1102 20-21 0.14859920278509264 1102 22-23 0.37135110260562953 1102 24-25 0.24697241148395932 1102 26-27 0.3736983712850517 1102 28-29 0.39094745899529215 1102 30-31 0.3005599923838247 1102 32-33 0.26060960136655353 1102 34-35 0.5592587553902959 1102 36-37 0.6203089648337965 1102 38-39 0.5511827304514085 1102 40-41 0.669811376324212 1102 42-43 0.6550646877422892 1102 44-45 0.6937252971051677 1102 46-47 0.7425305819799277 1102 48-49 0.881768939067598 1102 50-51 0.8508667402132986 1102 52-53 0.9197403298365217 1102 54-55 0.8824173440672993 1102 56-57 1.0084507487051795 1102 58-59 0.8983944785195703 1102 60-61 0.8698775576740871 1102 62-63 0.8688794281424492 1102 64-65 0.9560476186751217 1102 66-67 1.1556587423060378 1102 68-69 1.134676023581708 1102 70-71 1.2165490314723613 1102 72-73 1.1593201131818311 1102 74-75 1.1303708078170693 1102 76-77 1.1853135752722572 1102 78-79 1.2261843258411353 1102 80-81 1.4240201783433406 1102 82-83 1.1950071929435353 1102 84-85 1.5209423793631984 1102 86-87 1.5626105022646435 1102 88-89 1.3993212125376395 1102 90-91 1.3711697966024552 1102 92-93 1.32719971263003 1102 94-95 1.2737821239536764 1102 96-97 1.4387937581125598 1102 98-99 1.8774420557394578 1102 100-101 1.8024591274465678 1103 1 0.10765867653487149 1103 2 0.15237007163041838 1103 3 0.054395266811454235 1103 4 0.08133612025362424 1103 5 0.0481403708322361 1103 6 0.020087734409372615 1103 7 0.006834062548577435 1103 8 0.05655016004832447 1103 9 -0.019721860017284598 1103 10-11 0.1959978233577715 1103 12-13 0.025992544157986686 1103 14-15 0.18698967326324833 1103 16-17 0.20896687539837444 1103 18-19 0.2664618939957748 1103 20-21 0.18209213052254825 1103 22-23 0.13474450535794347 1103 24-25 0.04604947307294083 1103 26-27 0.08348200730797117 1103 28-29 0.2182427870651722 1103 30-31 0.06451355418057858 1103 32-33 0.055247147910677086 1103 34-35 0.23132077212581947 1103 36-37 0.2732227084887171 1103 38-39 0.5300122832204224 1103 40-41 0.5906145629162509 1103 42-43 0.485258584249344 1103 44-45 0.6307629112757596 1103 46-47 0.6222644351408562 1103 48-49 0.5361131252278 1103 50-51 0.5710224847970693 1103 52-53 0.4268752283560744 1103 54-55 0.614733632270152 1103 56-57 1.0190077097507029 1103 58-59 0.6560803698446378 1103 60-61 0.7498650101114919 1103 62-63 0.6117409649744303 1103 64-65 0.5961230262672075 1103 66-67 0.6192397474146887 1103 68-69 0.7008450653227278 1103 70-71 0.9247825546486865 1103 72-73 0.9751556056736668 1103 74-75 0.649412650679217 1103 76-77 0.6847574901117888 1103 78-79 0.7732381948087301 1103 80-81 0.8033940020093482 1103 82-83 0.8211850546433226 1103 84-85 0.7416664307593201 1103 86-87 0.6237554266126217 1103 88-89 0.7404733476602203 1103 90-91 0.7055241471031373 1103 92-93 0.9471613366431306 1103 94-95 0.8055889729673886 1103 96-97 0.6014995910996142 1103 98-99 0.8855806574195313 1103 100-101 0.6711777076165326 1104 1 0.10900820420018675 1104 2 0.402313841311031 1104 3 0.44154101579931293 1104 4 0.3753082300152073 1104 5 0.4407966911201342 1104 6 0.4881489129968699 1104 7 0.4656172384370123 1104 8 0.4523553782219665 1104 9 0.43956738874565104 1104 10-11 0.5597798746398155 1104 12-13 0.5062557020527194 1104 14-15 0.7017220169429592 1104 16-17 0.7034844192580252 1104 18-19 0.6718543816251099 1104 20-21 0.653049170558532 1104 22-23 0.6191405917277137 1104 24-25 0.5103432202614258 1104 26-27 0.6239116069480914 1104 28-29 0.46888943573813435 1104 30-31 0.6695799059574554 1104 32-33 0.5234207871369421 1104 34-35 0.5126691751847474 1104 36-37 0.6559543774045977 1104 38-39 0.7778474159239792 1104 40-41 0.9357281481614095 1104 42-43 0.62535642500508 1104 44-45 0.8964792855447641 1104 46-47 1.0936432025722524 1104 48-49 1.248495041557078 1104 50-51 1.0302555032406175 1104 52-53 1.0471799966871558 1104 54-55 1.223764221563897 1104 56-57 1.2499737466377923 1104 58-59 1.27138063975017 1104 60-61 1.2035312269355956 1104 62-63 1.3313203621854086 1104 64-65 1.4329988697219989 1104 66-67 1.4185818526778462 1104 68-69 1.46136013504832 1104 70-71 1.4113435083149 1104 72-73 1.385946203964263 1104 74-75 1.2651627631398554 1104 76-77 1.4050678814748103 1104 78-79 1.4800814246782785 1104 80-81 1.5312111410106972 1104 82-83 1.7264650816338758 1104 84-85 1.6884163182986818 1104 86-87 1.6892075183805026 1104 88-89 1.7855138334901817 1104 90-91 1.6576440301440698 1104 92-93 1.6742193663327392 1104 94-95 1.7350761524545675 1104 96-97 1.8571788533578228 1104 98-99 1.8835001356021657 1104 100-101 1.8822382114401925 1105 1 0.1985948721222286 1105 2 0.2901124971635376 1105 3 0.056194823843569 1105 4 0.10930556682772163 1105 5 -0.006573971672807488 1105 6 0.10308742206022004 1105 7 0.08023966211696631 1105 8 0.36961274345974715 1105 9 0.23624826804586974 1105 10-11 0.27459800224864495 1105 12-13 0.17360946212374273 1105 14-15 0.2513194003836787 1105 16-17 0.2195991694609063 1105 18-19 0.09183209872644937 1105 20-21 0.26882048614624665 1105 22-23 0.26048455987707086 1105 24-25 0.12342332660958988 1105 26-27 0.1034971988347877 1105 28-29 0.22653601192816808 1105 30-31 0.11061877325091274 1105 32-33 -0.014719061590419358 1105 34-35 0.2848691294532415 1105 36-37 0.38372864312255217 1105 38-39 0.5487124138391621 1105 40-41 0.6657789011619784 1105 42-43 0.370188092725364 1105 44-45 0.38159525077089285 1105 46-47 0.6805778917101222 1105 48-49 0.7482372414216769 1105 50-51 0.8493291264758014 1105 52-53 0.6668250395268203 1105 54-55 0.6996677549949695 1105 56-57 0.7311442489221278 1105 58-59 0.9789624296452999 1105 60-61 0.8135345447112527 1105 62-63 0.8257462749099744 1105 64-65 0.6486935177911874 1105 66-67 0.5256361610785447 1105 68-69 0.8770774246946225 1105 70-71 0.9712125458461216 1105 72-73 0.7966285448791588 1105 74-75 0.6034245625399635 1105 76-77 0.44610854216051266 1105 78-79 0.41725777342132986 1105 80-81 0.3880849893166136 1105 82-83 0.5297366774391286 1105 84-85 0.5778570489266812 1105 86-87 0.5340118368690305 1105 88-89 0.7174837403682872 1105 90-91 0.5717943433151902 1105 92-93 0.425162018132184 1105 94-95 0.514252402561052 1105 96-97 0.5785170826519916 1105 98-99 0.9814115061573574 1105 100-101 1.0493633139998124 1106 1 0.12002670066760146 1106 2 0.2629155718818019 1106 3 0.039199188380081296 1106 4 0.13648946364226333 1106 5 0.08168737937924675 1106 6 0.09846883043752541 1106 7 0.15380490216647758 1106 8 0.21960948634294652 1106 9 0.18188698865039044 1106 10-11 0.16098651114057816 1106 12-13 0.09797595290610417 1106 14-15 -0.025177244786014796 1106 16-17 -0.06771110348548603 1106 18-19 0.10530679092890693 1106 20-21 0.0623648806482322 1106 22-23 0.1636472202900734 1106 24-25 0.04334711007646064 1106 26-27 0.2750983974537675 1106 28-29 0.5700087498605413 1106 30-31 0.4449748412595085 1106 32-33 0.26389470949941085 1106 34-35 0.15819985709313045 1106 36-37 0.17638384473808344 1106 38-39 0.1767757321897534 1106 40-41 0.1437379917749695 1106 42-43 0.45869372753742255 1106 44-45 0.47714928633860865 1106 46-47 0.43871742659384694 1106 48-49 0.1561357496621838 1106 50-51 -0.04766781183440827 1106 52-53 0.02220579648076182 1106 54-55 0.3277929585647641 1106 56-57 0.174519273350505 1106 58-59 0.0573058600407208 1106 60-61 -0.08273931366930753 1106 62-63 0.10916429328010935 1106 64-65 0.1650898437634396 1106 66-67 0.1557970626484746 1106 68-69 -0.034436483194117073 1106 70-71 0.11257792920876852 1106 72-73 0.19363851165656953 1106 74-75 0.29809917657162543 1106 76-77 0.3357378822686492 1106 78-79 0.11317660607067026 1106 80-81 0.14387288587058578 1106 82-83 0.037983697177256914 1106 84-85 0.31686753684277846 1106 86-87 0.1467569349082467 1106 88-89 0.5954884306164807 1106 90-91 0.7677099188477285 1106 92-93 0.6426364497653019 1106 94-95 0.9545331660292291 1106 96-97 1.0244131154837284 1106 98-99 0.9972573793903692 1106 100-101 0.6930542787578062 1107 1 0.10327090744122103 1107 2 0.1844841993327826 1107 3 0.09606193347812209 1107 4 -0.032494493576990635 1107 5 0.06849664854145487 1107 6 0.20791624576729362 1107 7 0.18179064191691907 1107 8 0.19946688384740696 1107 9 0.3120117658339865 1107 10-11 0.14948918493736585 1107 12-13 0.07622907233569265 1107 14-15 0.29523273738866607 1107 16-17 0.18674522450025677 1107 18-19 0.3301731010893363 1107 20-21 0.3766251302026049 1107 22-23 0.20874525950576128 1107 24-25 0.20511181595880856 1107 26-27 0.3918541906801494 1107 28-29 0.09113174451294981 1107 30-31 0.0805363385857163 1107 32-33 0.294197739802442 1107 34-35 0.18404655941042591 1107 36-37 -0.1909067078465796 1107 38-39 0.1698238605319986 1107 40-41 0.20853300068763048 1107 42-43 0.004326526111398721 1107 44-45 0.002817735536474686 1107 46-47 -0.3789296322296849 1107 48-49 0.05916605269248976 1107 50-51 0.029961421677178635 1107 52-53 0.08602041323655385 1107 54-55 0.11371096212983645 1107 56-57 0.10767435356440558 1107 58-59 -0.016045298604559832 1107 60-61 -0.028906871601570572 1107 62-63 -0.03923142864502438 1107 64-65 -0.09908127923121413 1107 66-67 -0.13092307995402308 1107 68-69 -0.0778411177752254 1107 70-71 -0.2980281313972917 1107 72-73 -0.2074310070600056 1107 74-75 -0.14521633144976676 1107 76-77 -0.23146354375630906 1107 78-79 -0.172563144374962 1107 80-81 0.02337377713617883 1107 82-83 -0.15426229212755516 1107 84-85 -0.055770609324778064 1107 86-87 -0.15716463371920497 1107 88-89 -0.5769714624316435 1107 90-91 -0.4127713645747306 1107 92-93 -0.1326754931936982 1107 94-95 -0.21837235981747583 1107 96-97 -0.6568703069761668 1107 98-99 -0.24160180064528092 1107 100-101 0.12790276360628994 1108 1 0.1188878844853889 1108 2 0.0015388900615285195 1108 3 0.12650689834931583 1108 4 0.2272457661632714 1108 5 0.22228066587416606 1108 6 0.037431956671625244 1108 7 0.05943469506723886 1108 8 0.0687576702066579 1108 9 0.10676185663036364 1108 10-11 0.0849705309780191 1108 12-13 0.18130239823832994 1108 14-15 0.4246895871926739 1108 16-17 0.22508634336210775 1108 18-19 0.08699848399957943 1108 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-0.7972538363971609 2316 96-97 -0.7850202899854821 2316 98-99 0.00184784433889007 2316 100-101 0.49110988896175556 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2290.0 3 392.0 4 386.0 5 410.0 6 485.0 7 541.0 8 649.0 9 807.0 10 1038.0 11 1408.0 12 2041.0 13 2800.0 14 3434.0 15 4443.0 16 6157.0 17 8863.0 18 7649.0 19 4434.0 20 3907.0 21 3894.0 22 4220.0 23 4507.0 24 4917.0 25 5517.0 26 6357.0 27 7317.0 28 8609.0 29 10343.0 30 12693.0 31 16120.0 32 20589.0 33 27108.0 34 37233.0 35 54234.0 36 76763.0 37 105327.0 38 87204.0 39 12000.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.54282544964741 19.448826270789667 15.717671125325037 20.290677154237883 2 14.600920882578471 21.05465895304507 41.36105581095627 22.983364353420193 3 19.492625156361086 26.68244526237165 30.69104849668379 23.13388108458347 4 11.600810719351424 17.089546328362935 39.236008611193114 32.07363434109253 5 11.999438395394842 38.894538935219266 34.312961366283204 14.793061303102686 6 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33.606011938269184 20.047677540838844 28-29 19.586950187243012 27.39000147492095 33.073033768494966 19.95001456934107 30-31 19.844257547906228 26.49573187621182 32.98631970099751 20.67369087488444 32-33 19.803883124919057 26.616529722418086 33.005482566589436 20.574104586073418 34-35 20.71430562762543 26.606418750297895 32.50356802082087 20.175707601255798 36-37 20.24621244477934 26.14390956444801 33.35008798439749 20.25979000637516 38-39 20.039182860240906 26.9656839782741 32.857318824226475 20.13781433725851 40-41 20.051229443713694 27.272081571515784 32.64478971023627 20.031899274534258 42-43 19.84922409033606 27.11358727612457 31.864628985877346 21.17255964766202 44-45 20.051488777406803 27.0156921176131 31.72089953393934 21.211919571040756 46-47 20.863198084339764 27.612699251953742 31.277487485354243 20.246615178352247 48-49 20.613054109885326 27.592920847419062 30.853165770486022 20.940859272209586 50-51 19.971509997131214 28.03855143443747 31.336664960983622 20.65327360744769 52-53 20.527880867016982 28.536130804503824 30.061002045394446 20.874986283084752 54-55 20.08089367912474 27.79906427342711 30.56191252943816 21.558129518009995 56-57 20.711660457154366 27.957100421479637 30.112598357236358 21.21864076412964 58-59 21.305112450167503 27.485151399086156 30.17480260306808 21.034933547678257 60-61 20.695836667176106 27.62219844830221 31.265811404888836 20.416153479632843 62-63 20.246463836417202 27.918632950130945 31.093515827214592 20.74138738623726 64-65 20.041733570440908 28.42539832094752 30.620771127058134 20.91209698155344 66-67 20.24086940260936 27.818653893180283 30.166932780966356 21.773543923244006 68-69 20.140433527830474 27.809254947090185 30.495473221430046 21.554838303649294 70-71 20.78960003020947 27.78030522354109 30.117762988723594 21.312331757525843 72-73 20.477831041935634 27.48966983015007 30.740032150293633 21.292466977620663 74-75 20.044581279459344 28.711764674146632 30.00019781931184 21.243456227082184 76-77 20.08284235407509 27.81188833634679 30.131546177824255 21.973723131753868 78-79 20.346668321064712 27.79190909442387 30.23430412331739 21.627118461194026 80-81 20.578715400342737 27.920055528482624 29.88513084617999 21.61609822499465 82-83 20.40948599403642 27.747818043381134 30.443924456522225 21.398771506060214 84-85 20.27099428094734 28.025695284703104 30.06296171776538 21.640348716584175 86-87 20.1962432409351 28.1427011088687 29.992673465810256 21.668382184385944 88-89 20.148835101926334 27.975173772802624 29.456243055380753 22.41974806989029 90-91 20.228879982306708 27.727221236264775 29.746575970568724 22.297322810859797 92-93 20.92067362152482 27.941969728026926 29.76609165021451 21.37126500023375 94-95 20.38144983300894 27.78857088916558 29.70274242921422 22.127236848611254 96-97 19.853689498800676 27.863546622649448 30.211671036022913 22.071092842526962 98-99 20.424853937352655 28.338761043487015 29.032614813602663 22.203770205557664 100-101 20.526550005169305 27.35291076878818 29.642508776582595 22.478030449459922 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 907.0 1 586.5 2 364.0 3 452.0 4 371.0 5 323.0 6 570.5 7 1317.5 8 2204.5 9 2481.0 10 2054.5 11 1606.5 12 1532.0 13 1578.5 14 1636.0 15 1650.0 16 1618.0 17 1650.0 18 1704.5 19 1780.0 20 1959.0 21 2161.0 22 2404.5 23 2831.5 24 3369.5 25 4002.5 26 4839.5 27 5840.5 28 6571.5 29 7363.5 30 8439.0 31 9254.5 32 10204.5 33 11412.0 34 12677.0 35 13721.5 36 15177.5 37 16442.0 38 16721.5 39 16861.0 40 16967.0 41 17444.5 42 17762.0 43 18124.5 44 18991.5 45 19757.5 46 20039.0 47 22827.0 48 28563.0 49 27145.5 50 20405.5 51 16674.0 52 14991.5 53 14116.5 54 13186.0 55 11974.0 56 10557.5 57 9251.5 58 8140.5 59 7117.0 60 5899.0 61 4710.0 62 3681.0 63 2659.5 64 1887.0 65 1471.5 66 1122.0 67 855.5 68 640.0 69 423.5 70 301.0 71 238.0 72 208.5 73 194.0 74 146.5 75 107.5 76 81.0 77 60.0 78 43.5 79 36.0 80 26.0 81 15.5 82 12.0 83 9.0 84 6.5 85 4.5 86 2.5 87 2.0 88 4.5 89 4.0 90 2.5 91 2.0 92 1.0 93 1.5 94 2.5 95 2.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3162886879225111 2 0.2750024233242264 3 0.26584764291330243 4 0.2746434123277196 5 0.27554093981898664 6 0.20158467453858112 7 0.19260939962591053 8 0.19296841062241737 9 0.20553379550015619 10-11 0.2059825592457897 12-13 0.20293096577548173 14-15 0.2030207185246084 16-17 0.18596769619053433 18-19 0.1917118721346435 20-21 0.18022352024642516 22-23 0.19063483914512303 24-25 0.1918016248837702 26-27 0.19009632265036278 28-29 0.2017641800368345 30-31 0.19817407007176627 32-33 0.2049952790053959 34-35 0.1984433283191464 36-37 0.18336486646585987 38-39 0.1751076135462029 40-41 0.17241503107240175 42-43 0.18471115770276042 44-45 0.1871344819291815 46-47 0.18623695443791444 48-49 0.20023838330168053 50-51 0.19772530632613275 52-53 0.21594511439885405 54-55 0.18803200942044856 56-57 0.17053022334074092 58-59 0.1746588498005694 60-61 0.165324563891392 62-63 0.1686454156090801 64-65 0.16855566285995338 66-67 0.16765813536868635 68-69 0.17070972883899432 70-71 0.17394082780755574 72-73 0.1701712123442341 74-75 0.18363412471323998 76-77 0.17403058055668247 78-79 0.17815920701651092 80-81 0.1750178607970762 82-83 0.15733656921911518 84-85 0.15706731097173507 86-87 0.15949063519815612 88-89 0.16155494842807036 90-91 0.16927368485296704 92-93 0.16658110237916587 94-95 0.16487580014575848 96-97 0.16873516835820682 98-99 0.17753093777262396 100-101 0.16487580014575848 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 557086.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.79227942709359 #Duplication Level Percentage of deduplicated Percentage of total 1 82.22675543829668 50.80978648426516 2 10.690190972911411 13.211425354542708 3 3.0152030858157537 5.5894881482448575 4 1.3093532004133055 3.23631675314793 5 0.7158015421104876 2.2115504452217873 6 0.41266937878516863 1.5299868938938963 7 0.28615509575392095 1.2377522945419106 8 0.2076479361234226 1.0264831433118249 9 0.1621455680304484 0.9017409822842054 >10 0.9077166846358607 9.912307681147382 >50 0.040859891877554746 1.72386508709455 >100 0.01883611751125176 2.1971353585842164 >500 0.003477437079000099 1.4590268053580724 >1k 0.002897864232500082 3.9154492054442334 >5k 2.897864232500082E-4 1.037685362917284 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5795 1.0402343623785197 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4361 0.7828234778831276 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3223 0.5785462208707454 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3140 0.5636472645157121 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2749 0.4934606146986282 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2170 0.38952693120990295 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1696 0.3044413250377859 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1322 0.23730626869100999 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1105 0.1983535755700197 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1091 0.19584049859447195 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1009 0.1811210477376922 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 878 0.15760582746649532 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 820 0.14719450856779745 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 776 0.13929626664464734 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 727 0.13050049723023016 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 723 0.1297824752372165 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 682 0.12242274980882664 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 670 0.1202686838297857 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 643 0.1154220353769436 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 600 0.10770329895204689 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 588 0.10554923297300596 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 9.872802403937632E-4 0.0 26-27 0.0 0.0 0.0 0.001436043986027292 0.0 28-29 0.0 0.0 0.0 0.0026028297246744668 0.0 30-31 0.0 0.0 0.0 0.004756895703715404 0.0 32-33 0.0 0.0 0.0 0.007269972679263166 0.0 34-35 0.0 0.0 0.0 0.010860082644331397 0.0 36-37 0.0 0.0 0.0 0.013821923365512686 0.0 38-39 0.0 0.0 0.0 0.01750178607970762 0.0 40-41 0.0 0.0 0.0 0.024682006009844083 0.0 42-43 0.0 0.0 0.0 0.039132198619243705 0.0 44-45 0.0 0.0 0.0 0.06166013865004685 0.0 46-47 0.0 0.0 0.0 0.08894497438456539 0.0 48-49 0.0 0.0 0.0 0.1356164039304524 0.0 50-51 0.0 0.0 0.0 0.1839033829606201 0.0 52-53 0.0 0.0 0.0 0.2387423126770373 0.0 54-55 0.0 0.0 0.0 0.312339566960936 0.0 56-57 0.0 0.0 0.0 0.4011947885963747 0.0 58-59 0.0 0.0 0.0 0.512129186516983 0.0 60-61 0.0 0.0 0.0 0.6305130626151079 0.0 62-63 0.0 0.0 0.0 0.7816566921444803 0.0 64-65 0.0 0.0 0.0 0.9292102117087846 0.0 66-67 0.0 0.0 0.0 1.1035100505128472 0.0 68-69 0.0 0.0 0.0 1.3314820332946797 0.0 70-71 0.0 0.0 0.0 1.5489529444286878 0.0 72-73 0.0 0.0 0.0 1.794695971537608 0.0 74-75 0.0 0.0 0.0 2.0843280929694874 0.0 76-77 0.0 0.0 0.0 2.394244335704003 0.0 78-79 0.0 0.0 0.0 2.706942913661446 0.0 80-81 0.0 0.0 0.0 3.098175147104756 0.0 82-83 0.0 0.0 0.0 3.5213593592371737 0.0 84-85 0.0 0.0 0.0 3.937632609686835 0.0 86-87 0.0 0.0 0.0 4.385319322330844 0.0 88-89 0.0 0.0 0.0 4.941876119665546 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTATC 25 0.004996475 57.067432 1 CGTATCA 55 0.0025681104 34.573856 2 GATGCCG 65 0.005833477 29.238997 9 CCGGGAC 100 4.9239352E-5 28.520863 3 GAGTACT 3745 0.0 25.367447 12-13 GTGTATC 95 0.0012173364 25.029573 1 GTACATG 4055 0.0 24.745638 1 GTGTAGC 400 0.0 23.778097 1 GTTACGG 50 0.0016509041 23.756685 24-25 TACATGG 4140 0.0 23.654697 2 GTCTAGG 125 2.2358909E-4 22.826973 1 AGTACTT 3860 0.0 22.519686 12-13 GTACTTT 4165 0.0 22.468252 14-15 ACATGGG 4240 0.0 22.197842 3 GGTCGGG 130 2.9154538E-4 21.949013 1 GTCGGGA 130 2.9225697E-4 21.941101 2 ATGGGAT 1135 0.0 20.940428 5 CATGGGA 3005 0.0 20.565979 4 GTATCAA 8905 0.0 20.453701 1 GTCCATA 140 4.808895E-4 20.381224 1 >>END_MODULE