FastQCFastQC Report
Thu 26 May 2016
SRR937562_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937562_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557086
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59171.0621340331654359No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT57951.0402343623785197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50090.8991430407513382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35340.6343724308275562No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17650.3168272044172713No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15330.2751819288224798No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14000.2513076975547761No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12040.21612461989710743No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT12030.21594511439885405No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA11560.2075083559809437No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11440.20535429000190275No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT11380.20427725701238228No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG11140.1999691250543004No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA9420.16909417935471363No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9130.16388851990536468No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC8520.15293868451190662No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC8310.14916906904858496No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC6130.11003687042934124No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA5870.10536972747475254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG550.002575670134.553162
CCGATCG804.4895572E-429.6887919
GAGTACT43950.024.80483212-13
ACTATCC1351.3406612E-524.63078
GTGCTAT1000.001647849223.7616981
CGGGCCG1000.001650435523.7552975
GTACTTT46100.023.49132214-15
AGTACTT44900.023.3278512-13
GTACATG39300.021.6455151
TACATGG39900.021.076132
ACTTTTT52400.020.75762616-17
TACTTTT50350.020.70658914-15
ACACGCT1150.00371996720.6530729
ACTCCGG1150.00371996720.6530728
GTAAGGT1856.5295135E-620.5451224
AGAGTAC71700.020.53785110-11
GGTAAGG2108.5729516E-720.3616833
GGCCGAT1404.8460008E-420.3580277
TAGCGCG704.8531248E-420.35620128-29
TAAGGTG2601.4821126E-820.1006375
Produced by FastQC (version 0.11.5)