##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937562_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 557086 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13733427154874 33.0 31.0 34.0 31.0 34.0 2 32.3507088672126 34.0 31.0 34.0 31.0 34.0 3 32.4064130134306 34.0 31.0 34.0 31.0 34.0 4 36.05035847248001 37.0 35.0 37.0 35.0 37.0 5 35.974323533529834 37.0 35.0 37.0 35.0 37.0 6 36.04414399213048 37.0 35.0 37.0 35.0 37.0 7 35.99423248834112 37.0 35.0 37.0 35.0 37.0 8 35.978949390219825 37.0 35.0 37.0 35.0 37.0 9 37.62602363010379 39.0 37.0 39.0 35.0 39.0 10-11 37.55084852249024 39.0 37.0 39.0 35.0 39.0 12-13 37.45028954236868 39.0 37.0 39.0 35.0 39.0 14-15 38.53017307920142 40.0 38.0 41.0 34.0 41.0 16-17 38.53767102386346 40.0 38.0 41.0 34.0 41.0 18-19 38.60461041921714 40.0 38.0 41.0 34.0 41.0 20-21 38.536849786208954 40.0 38.0 41.0 34.0 41.0 22-23 38.3717271660031 40.0 38.0 41.0 34.0 41.0 24-25 38.40542932330017 40.0 38.0 41.0 34.0 41.0 26-27 38.235552499973075 40.0 38.0 41.0 34.0 41.0 28-29 38.08984698951329 40.0 37.0 41.0 34.0 41.0 30-31 38.07800590932101 40.0 37.0 41.0 34.0 41.0 32-33 37.90957769536481 40.0 37.0 41.0 33.5 41.0 34-35 37.711277073916776 40.0 36.0 41.0 33.0 41.0 36-37 37.49192674021606 40.0 36.0 41.0 33.0 41.0 38-39 37.31377740600195 40.0 36.0 41.0 33.0 41.0 40-41 37.110462298460206 40.0 36.0 41.0 32.5 41.0 42-43 36.81269498784748 39.0 35.0 41.0 31.5 41.0 44-45 36.36808051180608 39.0 35.0 41.0 30.5 41.0 46-47 35.83138510032562 39.0 35.0 41.0 29.0 41.0 48-49 35.3229600097651 39.0 35.0 40.0 26.5 41.0 50-51 34.99267168803381 39.0 35.0 40.0 25.0 41.0 52-53 34.7479437645175 38.5 35.0 40.0 24.0 41.0 54-55 34.52997652068083 38.0 34.0 40.0 23.5 41.0 56-57 34.24110640008904 38.0 34.0 40.0 23.0 41.0 58-59 33.957275896360706 38.0 34.0 40.0 22.0 41.0 60-61 33.53059850723228 37.5 33.0 40.0 20.0 41.0 62-63 33.21220152723278 37.0 33.0 40.0 18.5 41.0 64-65 32.81668898518362 36.5 32.5 39.0 17.5 41.0 66-67 32.364101772437294 36.0 32.0 39.0 13.0 40.0 68-69 31.881693311266126 35.5 31.0 39.0 8.0 40.0 70-71 31.410630495112066 35.0 31.0 38.0 7.5 40.0 72-73 30.8895269312099 35.0 30.5 37.0 4.5 39.5 74-75 30.373720574561197 34.5 30.0 37.0 2.0 39.0 76-77 29.19350154195223 33.5 28.5 35.5 2.0 38.0 78-79 29.78791066370363 34.0 30.0 36.0 2.0 38.0 80-81 29.55735739185691 34.0 29.0 35.5 2.0 37.0 82-83 29.15987477696442 34.0 29.0 35.0 2.0 37.0 84-85 28.739136327245703 34.0 29.0 35.0 2.0 36.5 86-87 28.310090363067822 34.0 29.0 35.0 2.0 36.0 88-89 27.898398092933586 33.5 27.5 35.0 2.0 36.0 90-91 27.316576794247208 33.0 26.0 35.0 2.0 35.0 92-93 26.777211238480234 33.0 25.5 35.0 2.0 35.0 94-95 26.21692880452928 32.0 25.0 34.5 2.0 35.0 96-97 25.564584283216597 32.0 22.5 34.0 2.0 35.0 98-99 24.60888265007557 32.0 10.0 34.0 2.0 35.0 100-101 21.989663175883077 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2949724021893303 1101 2 0.2232756989323761 1101 3 0.31228743541274184 1101 4 0.19315271523459643 1101 5 0.16280826837609652 1101 6 0.12113480605730587 1101 7 0.24103035752749946 1101 8 0.27860233637286314 1101 9 0.16006309967505672 1101 10-11 0.2057941811717754 1101 12-13 0.3660134869269811 1101 14-15 0.45604407187774143 1101 16-17 0.4482545259565569 1101 18-19 0.41998028940604115 1101 20-21 0.4321130588071185 1101 22-23 0.29566743906944026 1101 24-25 0.3672078850496092 1101 26-27 0.32179789432084505 1101 28-29 0.4628942582424145 1101 30-31 0.5307564941710936 1101 32-33 0.27893181638798836 1101 34-35 0.39638196616049015 1101 36-37 0.3491957785080544 1101 38-39 0.36628610035066345 1101 40-41 0.436167107177333 1101 42-43 0.5575896317295488 1101 44-45 0.6960391180664303 1101 46-47 0.6430076680507142 1101 48-49 0.6308319824971917 1101 50-51 0.6848628292098695 1101 52-53 0.6780946309181317 1101 54-55 0.7646196501538611 1101 56-57 0.804890476317091 1101 58-59 0.9857790805572861 1101 60-61 0.716150865743785 1101 62-63 1.0342076571735888 1101 64-65 0.9647883546483627 1101 66-67 0.941555514981566 1101 68-69 1.0566541906995752 1101 70-71 1.0728695545847984 1101 72-73 0.9861701868372315 1101 74-75 1.041854094848965 1101 76-77 0.7076077019244558 1101 78-79 0.9706700956771286 1101 80-81 1.1396842321163838 1101 82-83 1.1003797187280568 1101 84-85 0.934016689186759 1101 86-87 0.7447863434333293 1101 88-89 1.015771561678978 1101 90-91 1.1364243168425538 1101 92-93 1.2020820260989282 1101 94-95 1.3900920566354635 1101 96-97 1.460157779675935 1101 98-99 1.5311168907840873 1101 100-101 1.9767001825334383 1102 1 0.21464704280977287 1102 2 0.22019517963990864 1102 3 0.1907224212857912 1102 4 0.06365109740171704 1102 5 0.05239311366921129 1102 6 0.08850578022197197 1102 7 0.06740249873836746 1102 8 0.09540194057698415 1102 9 0.23181834060890338 1102 10-11 0.15712559643508683 1102 12-13 0.2759841470062412 1102 14-15 0.24512245014896905 1102 16-17 0.39664031316110737 1102 18-19 0.23738668868064394 1102 20-21 0.2610235686011748 1102 22-23 0.08869989743416085 1102 24-25 0.04131411211163538 1102 26-27 0.19786086310020323 1102 28-29 0.08216870717077995 1102 30-31 0.011857930654990412 1102 32-33 0.02812289994814421 1102 34-35 0.22050060493986479 1102 36-37 0.40019026736078445 1102 38-39 0.31203224280615416 1102 40-41 0.3668239628411243 1102 42-43 0.3646697407457964 1102 44-45 0.41473185393646617 1102 46-47 0.7244332744579154 1102 48-49 0.8131540769055405 1102 50-51 0.7688660217793952 1102 52-53 0.5695067568212195 1102 54-55 0.7233528447747517 1102 56-57 0.4334245835439887 1102 58-59 0.5055559454609124 1102 60-61 0.581669046323924 1102 62-63 0.7481628232923541 1102 64-65 0.619049624746296 1102 66-67 0.6154182482830706 1102 68-69 0.9150261526300767 1102 70-71 0.8093476780710418 1102 72-73 0.7523136076789179 1102 74-75 0.6016001373211708 1102 76-77 0.5193909036993283 1102 78-79 0.8930852262501183 1102 80-81 0.6316997915831628 1102 82-83 0.6989468549139026 1102 84-85 0.6233091695822814 1102 86-87 0.8790444933634234 1102 88-89 1.0846966724794136 1102 90-91 0.8527058180397695 1102 92-93 0.8353825775584234 1102 94-95 0.9139354828210848 1102 96-97 1.1175310293221123 1102 98-99 1.1491385319923673 1102 100-101 1.6203240075144336 1103 1 0.19761107856218274 1103 2 0.21670895344157515 1103 3 0.12941352310844678 1103 4 0.07272567392147522 1103 5 0.04363120774333851 1103 6 0.0647407781442908 1103 7 0.03850643981370894 1103 8 0.07377165369407379 1103 9 0.07472631971802457 1103 10-11 0.11409061399353249 1103 12-13 0.10471246375886523 1103 14-15 0.18074693582111223 1103 16-17 0.3255719167233124 1103 18-19 0.1618940542628451 1103 20-21 0.03276741499945501 1103 22-23 0.3812495784490437 1103 24-25 0.2750532042873388 1103 26-27 0.11881223722126322 1103 28-29 0.279549912714522 1103 30-31 0.36573586079602904 1103 32-33 0.30365902307721626 1103 34-35 0.43061829944745256 1103 36-37 0.4120396680924543 1103 38-39 0.49851937340672237 1103 40-41 0.3970689865918686 1103 42-43 0.5968027041704644 1103 44-45 0.7001715889710525 1103 46-47 0.7878218903965859 1103 48-49 0.9017590160824582 1103 50-51 0.9705838767980097 1103 52-53 0.9453296813810326 1103 54-55 0.912175329355108 1103 56-57 1.017206553348366 1103 58-59 0.9709213117438225 1103 60-61 0.8554213175142351 1103 62-63 1.0314265950570771 1103 64-65 1.1661849713930792 1103 66-67 1.360573381784242 1103 68-69 1.314900778851225 1103 70-71 1.446860103417123 1103 72-73 1.2863249371502086 1103 74-75 1.0475804360183432 1103 76-77 0.761622516224076 1103 78-79 1.0116393233552152 1103 80-81 1.0359793048355037 1103 82-83 1.1342393190433562 1103 84-85 1.0582487335137394 1103 86-87 1.0574785738470744 1103 88-89 0.8921007528815856 1103 90-91 1.0920113634106166 1103 92-93 1.1873681886002814 1103 94-95 1.2802367220965856 1103 96-97 1.47399740348391 1103 98-99 1.4589696287634055 1103 100-101 1.4955455249547285 1104 1 0.04584544653339151 1104 2 0.08023796828638297 1104 3 0.00430106246967199 1104 4 0.016382893894487438 1104 5 0.11251334899390031 1104 6 0.18855994143713417 1104 7 0.1624380637453342 1104 8 0.14945766359060997 1104 9 0.19848925269148765 1104 10-11 0.20403100985497957 1104 12-13 0.14297719610254234 1104 14-15 0.333693404557053 1104 16-17 0.24496575388030806 1104 18-19 0.19937156213509155 1104 20-21 0.2460771315986392 1104 22-23 0.2051362181251548 1104 24-25 0.07532310982039547 1104 26-27 0.17622339331663284 1104 28-29 0.18887852270102456 1104 30-31 0.11404895121438585 1104 32-33 0.13761088992381332 1104 34-35 0.09475460174164851 1104 36-37 0.2258329654383786 1104 38-39 0.08674474452682546 1104 40-41 0.4532149605009934 1104 42-43 0.5877496227489658 1104 44-45 0.7084374459211134 1104 46-47 0.5540724526997778 1104 48-49 0.6513091735273449 1104 50-51 0.6901913891686817 1104 52-53 0.5766274771525133 1104 54-55 0.6549778484734148 1104 56-57 0.7099922033708523 1104 58-59 0.5954995843484099 1104 60-61 0.5878493427054181 1104 62-63 0.682376887454744 1104 64-65 0.8080259520498458 1104 66-67 0.7384973583924292 1104 68-69 0.8675604729582886 1104 70-71 1.0049179081854547 1104 72-73 0.7352959223054043 1104 74-75 0.6670136343989128 1104 76-77 0.5736164433495823 1104 78-79 0.7777211947002449 1104 80-81 0.3682723772601584 1104 82-83 0.8437534890838414 1104 84-85 0.753339826631148 1104 86-87 0.8826022805497296 1104 88-89 0.8276045765433047 1104 90-91 0.8160048406935694 1104 92-93 0.8899097990366265 1104 94-95 1.261680716698475 1104 96-97 1.5760835483332123 1104 98-99 1.0642327866581418 1104 100-101 1.4812911389898176 1105 1 0.1314037071222529 1105 2 0.12169447179918791 1105 3 0.10275381330920652 1105 4 -0.08961779656152657 1105 5 -0.01674091367529229 1105 6 -0.08810443865441187 1105 7 0.07388707984551246 1105 8 0.07710101167459982 1105 9 0.15880148228156088 1105 10-11 0.05620841221597317 1105 12-13 0.2393260736631717 1105 14-15 0.1811586687909852 1105 16-17 0.365366263203704 1105 18-19 0.43511969812802676 1105 20-21 0.4544121462372033 1105 22-23 0.2764067468620297 1105 24-25 0.058595243643331685 1105 26-27 -0.07483358723851552 1105 28-29 0.08144423931815226 1105 30-31 0.0925652329742448 1105 32-33 0.24832708111735968 1105 34-35 0.2525260428668261 1105 36-37 0.1723170199395696 1105 38-39 0.049599107625994066 1105 40-41 0.2384506346595643 1105 42-43 0.4862346546489249 1105 44-45 0.5514472512932258 1105 46-47 0.4767895764572927 1105 48-49 0.5215023218722692 1105 50-51 0.6537429335035796 1105 52-53 0.1258426438707403 1105 54-55 0.441046329156336 1105 56-57 0.46530512790042877 1105 58-59 0.537126908472672 1105 60-61 0.6928950517902948 1105 62-63 0.30110281676818573 1105 64-65 0.36396409795128193 1105 66-67 0.595924916554381 1105 68-69 0.5038134488685486 1105 70-71 0.917249049096764 1105 72-73 0.5936179348501831 1105 74-75 0.516295829152341 1105 76-77 0.6701166383809927 1105 78-79 0.5962220532867839 1105 80-81 0.2869465223724923 1105 82-83 0.271623252938916 1105 84-85 0.32074447420715657 1105 86-87 0.3188012305185701 1105 88-89 0.3974213366905417 1105 90-91 0.5019359026956174 1105 92-93 0.5070149501075036 1105 94-95 0.184721771930473 1105 96-97 0.36225626994045257 1105 98-99 0.3105966838788312 1105 100-101 0.448208656396929 1106 1 0.09181670953251597 1106 2 0.03198044665424504 1106 3 0.12113046629598045 1106 4 0.20915101328799324 1106 5 0.022904712016838857 1106 6 -0.031111408884370917 1106 7 0.05610322211636998 1106 8 -0.05244755193689343 1106 9 -0.0034159628360228567 1106 10-11 -0.05498304110953711 1106 12-13 -0.11784284041409165 1106 14-15 0.16297418569541833 1106 16-17 0.15902465679369726 1106 18-19 0.08018842329251186 1106 20-21 0.14164750549718974 1106 22-23 0.2323053853872068 1106 24-25 0.3559732646191591 1106 26-27 0.033530688704423994 1106 28-29 0.05569998812930521 1106 30-31 0.2109093147930139 1106 32-33 0.2241592744598151 1106 34-35 -0.05878592378029879 1106 36-37 0.06396902958064032 1106 38-39 -0.1186374893383757 1106 40-41 -0.018171083795259335 1106 42-43 -0.12203842202359994 1106 44-45 0.08603131747783976 1106 46-47 0.3084226948209192 1106 48-49 0.2597278446395208 1106 50-51 0.07740262994028768 1106 52-53 0.13810243150672363 1106 54-55 0.0737640674144302 1106 56-57 0.11825607572141905 1106 58-59 -0.007905988407003406 1106 60-61 0.2415010057998046 1106 62-63 0.23187279917976156 1106 64-65 0.2431771785323491 1106 66-67 0.3286100836444703 1106 68-69 0.08379846613126674 1106 70-71 0.24509414062174528 1106 72-73 0.3129652086434227 1106 74-75 0.11228631837128944 1106 76-77 0.013149665545345357 1106 78-79 -0.06960753436223044 1106 80-81 -0.33932863885479136 1106 82-83 -0.5144786296745956 1106 84-85 -0.6499903313429733 1106 86-87 -0.5601936232368878 1106 88-89 -0.8297821028247974 1106 90-91 -0.8308078924968747 1106 92-93 -0.8345523744967522 1106 94-95 -0.3981946504524352 1106 96-97 -0.6062376392511304 1106 98-99 -0.08831543910989836 1106 100-101 -0.09764829103320238 1107 1 -0.005865999914895781 1107 2 -0.05768087241883535 1107 3 -0.08136507737603438 1107 4 0.044445130935052646 1107 5 -0.030749476931461572 1107 6 -0.07371390442803971 1107 7 -0.037479665584164934 1107 8 -0.11180584070694977 1107 9 -0.02355856533156242 1107 10-11 -0.18127537622540757 1107 12-13 -0.08326173524474711 1107 14-15 -0.19772100147035587 1107 16-17 -0.15327480844694463 1107 18-19 -0.022663626618367516 1107 20-21 -0.10255926812134675 1107 22-23 -0.1322222438463072 1107 24-25 0.08181996693645033 1107 26-27 -0.1362554075522624 1107 28-29 -0.11640339867996374 1107 30-31 -0.19274487415528796 1107 32-33 -0.21523466493412258 1107 34-35 -0.2509427865253926 1107 36-37 -0.549221701257153 1107 38-39 -0.399207899320551 1107 40-41 -0.41139746525693255 1107 42-43 -0.5692754986724466 1107 44-45 -0.4494410532886448 1107 46-47 -0.3620764139134849 1107 48-49 0.13405939544166046 1107 50-51 -0.3104761579384956 1107 52-53 -0.735248727138547 1107 54-55 -0.5052911910525921 1107 56-57 -0.4814765445994382 1107 58-59 -0.3393676639863301 1107 60-61 -0.4410301884961001 1107 62-63 -0.18925019547265265 1107 64-65 0.002446340742686459 1107 66-67 -0.28850221582076685 1107 68-69 -0.6628147245995706 1107 70-71 -0.47638536026951783 1107 72-73 -0.2239331870785044 1107 74-75 -0.4292466584558099 1107 76-77 -0.5125187836525171 1107 78-79 -0.4169337375707869 1107 80-81 -0.10456928056602166 1107 82-83 -0.23872105391701837 1107 84-85 -0.4975839142306704 1107 86-87 -0.21001537011745697 1107 88-89 -0.10464841298522742 1107 90-91 -0.5479201919621168 1107 92-93 -0.7340176151384661 1107 94-95 -0.5807258447483328 1107 96-97 -0.40106829878767414 1107 98-99 -0.3179054569352111 1107 100-101 -0.08347716803855221 1108 1 0.2156583773471823 1108 2 0.07096194089128716 1108 3 0.05635111193128495 1108 4 9.818439809237134E-4 1108 5 -0.0696792067965859 1108 6 -0.029311344596358424 1108 7 -0.045632554161350924 1108 8 -0.004947896336950919 1108 9 0.12448962562887544 1108 10-11 -0.06472726666861206 1108 12-13 0.036818040331652924 1108 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0.8486121646452816 2316 58-59 0.9330868635832843 2316 60-61 1.0333988351656629 2316 62-63 1.042319385841175 2316 64-65 1.0502661679742715 2316 66-67 0.8039494501603102 2316 68-69 0.8350837963168516 2316 70-71 0.7808669781848643 2316 72-73 0.8408817933374984 2316 74-75 0.9578368462747555 2316 76-77 0.7810623155551824 2316 78-79 0.8118994497022989 2316 80-81 0.6621173576039929 2316 82-83 1.0520208129118025 2316 84-85 0.6890318996229929 2316 86-87 0.6802024683101031 2316 88-89 0.5164504370138765 2316 90-91 0.8962430616640553 2316 92-93 0.8310879953635641 2316 94-95 0.8479624746049446 2316 96-97 0.9631046126998193 2316 98-99 0.7543988372360921 2316 100-101 0.7137032730037802 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 5.0 5 12.0 6 29.0 7 70.0 8 167.0 9 321.0 10 598.0 11 1034.0 12 1602.0 13 2363.0 14 3169.0 15 4504.0 16 6633.0 17 9370.0 18 8385.0 19 4696.0 20 3748.0 21 3473.0 22 3706.0 23 3926.0 24 4478.0 25 5103.0 26 6143.0 27 7295.0 28 9175.0 29 10988.0 30 13685.0 31 17122.0 32 21869.0 33 28559.0 34 39604.0 35 56203.0 36 82033.0 37 112372.0 38 78688.0 39 5957.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.210418589285524 20.317218645303303 16.14622795646413 19.326134808947046 2 15.45703894910301 22.671005912911113 39.78936824834945 22.08258688963643 3 20.38895251361549 26.83355891190947 30.752702455276204 22.02478611919883 4 12.266530242243105 18.117881472306742 38.58998178015922 31.02560650529093 5 12.113584378557142 39.824373673645994 33.633464876530134 14.42857707126673 6 28.913489120171747 38.745902787002365 16.968475244396735 15.372132848429148 7 26.626409566925034 35.647637887148484 20.369745425302376 17.356207120624102 8 26.363434011983788 33.82906768434317 19.43613732888638 20.371360974786658 9 26.519065278969496 17.959166089257312 21.096383682232187 34.425384949541005 10-11 24.820584254495714 28.078519295046007 26.24208829516448 20.858808155293794 12-13 26.986945029103293 26.08256629582591 27.299833503121256 19.63065517194954 14-15 22.690392506722482 26.842175175825634 28.444261747737333 22.02317056971455 16-17 19.80538013879365 30.3356214300844 28.479265319896747 21.379733111225196 18-19 19.73214189550626 28.40791188434102 32.4676979855893 19.392248234563425 20-21 21.608692374247422 25.93504414040202 32.64433139587066 19.8119320894799 22-23 20.7217807839262 26.568691598431464 32.76878296763215 19.940744650010185 24-25 21.100602061441144 26.714995530313097 32.88486876353023 19.299533644715538 26-27 20.47165577654915 26.62669634522869 33.698122352265386 19.203525525956774 28-29 19.627858467382193 28.16946791533605 32.99827850209937 19.20439511518239 30-31 20.216178471546584 26.766693113810074 32.89878043964487 20.118347974998475 32-33 20.05641857810105 27.150386116326743 32.853455301335885 19.93974000423633 34-35 20.969742553214406 26.761936218106356 32.812079283988474 19.456241944690767 36-37 20.72382002060723 26.513321103025383 33.29001267308818 19.472846203279207 38-39 20.091170924391317 27.404680251056618 33.115204039595355 19.388944784956706 40-41 20.137682817920307 27.673977139625993 32.44045936157895 19.747880680874744 42-43 20.035117831455953 27.767773464851647 31.672747054662448 20.524361649029952 44-45 20.76555504895115 27.112689961693526 31.591980412360034 20.529774576995294 46-47 21.44067522788223 28.121151850881194 30.91766800817109 19.520504913065487 48-49 21.19385516778379 28.320223448444224 30.457954427144102 20.027966956627882 50-51 20.33833196310803 28.83863532740008 30.877728034809703 19.945304674682184 52-53 21.0050153836212 29.329941876119666 29.41834833400947 20.246694406249663 54-55 20.406005535949568 28.80722186520573 29.732841966949447 21.053930631895255 56-57 21.329651077212493 28.470559303231457 29.775384770035508 20.424404849520542 58-59 22.016169855282666 28.020808277357535 29.498228280732242 20.464793586627557 60-61 20.875861177627872 28.50996076009808 30.817503940145684 19.796674122128362 62-63 20.436611223401773 28.452698506155244 30.680810503225715 20.429879767217273 64-65 20.47726921875617 29.28811709502662 29.9276054325544 20.307008253662808 66-67 20.851538182614533 28.46616141852425 29.425887564936115 21.256412833925104 68-69 20.641086511855004 28.644256581799382 29.627139819650665 21.08751708669495 70-71 21.434841299188996 28.626459828464547 29.433785806859262 20.504913065487195 72-73 21.110564616594207 28.112445834215904 30.211672883540423 20.56531666564947 74-75 20.3960429807965 29.758690758697938 29.070466678394357 20.7747995821112 76-77 20.66467295893273 28.501254743432792 29.433785806859262 21.400286490775215 78-79 20.787275214239813 28.826428953518846 29.290719924751297 21.095575907490048 80-81 21.078702390654225 28.687671203368957 29.26280681977289 20.970819586203927 82-83 20.915773601163227 28.44884843915486 29.685766600247725 20.949611359434183 84-85 20.4598939120601 29.083892045199566 29.42926124379583 21.026952798944507 86-87 20.453753998484974 29.009793819984708 28.981970467755424 21.55448171377489 88-89 20.275864049715842 28.89500005385165 28.746100243050442 22.083035653382062 90-91 20.803183434364108 28.397950839170317 28.909253628984118 21.889612097481457 92-93 21.199607233509372 28.80658583958325 29.02836966004517 20.965437266862214 94-95 20.582908204478304 28.86179153667477 28.807939887198746 21.747360371648185 96-97 20.07840800163709 28.92111810384752 29.38890943229591 21.61156446221948 98-99 20.641853109413553 29.29249139926527 27.992306235521458 22.07334925579972 100-101 20.305170957319696 28.651633446535836 28.478732562909975 22.564463033234496 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 748.0 1 520.5 2 381.0 3 490.0 4 422.0 5 386.0 6 637.0 7 1304.0 8 1965.0 9 2280.0 10 2056.5 11 1680.0 12 1645.5 13 1704.0 14 1807.0 15 1871.5 16 1877.5 17 1866.5 18 1914.0 19 1974.0 20 2065.5 21 2272.5 22 2577.0 23 2951.0 24 3404.5 25 4017.0 26 4864.0 27 5794.5 28 6497.5 29 7376.0 30 8523.5 31 9439.0 32 10294.5 33 11344.0 34 12613.0 35 13682.5 36 15146.5 37 16415.5 38 16727.5 39 16687.0 40 16829.0 41 17485.5 42 17829.0 43 18208.0 44 19026.5 45 19601.0 46 19509.5 47 22530.0 48 29384.5 49 27375.0 50 19746.5 51 16593.0 52 14966.5 53 13992.5 54 13096.0 55 11836.5 56 10334.0 57 9095.0 58 8136.5 59 7158.0 60 5900.5 61 4673.5 62 3705.5 63 2768.5 64 1929.0 65 1386.0 66 992.5 67 749.0 68 567.0 69 388.5 70 285.5 71 229.5 72 191.0 73 169.5 74 134.5 75 111.5 76 97.0 77 71.5 78 42.5 79 32.5 80 20.5 81 16.0 82 18.0 83 12.5 84 7.0 85 3.5 86 1.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02154065979040938 2 0.0 3 0.0 4 1.795054982534115E-4 5 0.019386593811368442 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0033208517176881126 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.872802403937632E-4 24-25 0.0 26-27 0.001077032989520469 28-29 0.0023335714772943494 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.975274912670575E-5 40-41 0.0015257967351539978 42-43 0.018668571818354796 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 8.975274912670575E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.002872087972054584 84-85 1.795054982534115E-4 86-87 0.0 88-89 0.0 90-91 0.013911676114639391 92-93 0.002154065979040938 94-95 0.0 96-97 0.0 98-99 0.002064313229914232 100-101 0.021450907041282673 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 557086.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.54624145548797 #Duplication Level Percentage of deduplicated Percentage of total 1 81.14360582279461 48.31796744893067 2 11.105329362154908 13.225612472831926 3 3.299441840573283 5.89408081521349 4 1.4399859657111238 3.429830080269944 5 0.7491704954894427 2.2305143607870956 6 0.4868667974364359 1.7394652726086097 7 0.32219639476982315 1.3429909023336124 8 0.2374767580763291 1.1312678701183676 9 0.17585194710366914 0.9424190252387502 >10 0.9607218603637186 10.184248855665215 >50 0.048693737859324376 2.0083713055496064 >100 0.024647445575709793 2.896307139985158 >500 0.002104050232072722 0.945210604762166 >1k 0.003005786045818174 2.718934727692116 >5k 9.017358137454523E-4 2.9927791180132886 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5917 1.0621340331654359 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5795 1.0402343623785197 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5009 0.8991430407513382 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3534 0.6343724308275562 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1765 0.3168272044172713 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1533 0.2751819288224798 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1400 0.2513076975547761 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1204 0.21612461989710743 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1203 0.21594511439885405 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1156 0.2075083559809437 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1144 0.20535429000190275 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1138 0.20427725701238228 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1114 0.1999691250543004 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 942 0.16909417935471363 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 913 0.16388851990536468 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 852 0.15293868451190662 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 831 0.14916906904858496 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 613 0.11003687042934124 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 587 0.10536972747475254 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.795054982534115E-4 0.0 16-17 0.0 0.0 0.0 1.795054982534115E-4 0.0 18-19 0.0 0.0 0.0 1.795054982534115E-4 0.0 20-21 0.0 0.0 0.0 1.795054982534115E-4 0.0 22-23 0.0 0.0 0.0 1.795054982534115E-4 0.0 24-25 0.0 0.0 0.0 0.0011667857386471747 0.0 26-27 0.0 0.0 0.0 0.0016155494842807036 0.0 28-29 0.0 0.0 0.0 0.0027823352229278783 0.0 30-31 0.0 0.0 0.0 0.0052056594493489336 0.0 32-33 0.0 0.0 0.0 0.007987994672276812 0.0 34-35 0.0 0.0 0.0 0.011308846389964926 0.0 36-37 0.0 0.0 0.0 0.014360439860272921 0.0 38-39 0.0 0.0 0.0 0.017950549825341153 0.0 40-41 0.0 0.0 0.0 0.025220522504604315 0.0 42-43 0.0 0.0 0.0 0.03967071511400394 0.0 44-45 0.0 0.0 0.0 0.062378160643060496 0.0 46-47 0.0 0.0 0.0 0.08993225462495916 0.0 48-49 0.0 0.0 0.0 0.1371422006656064 0.0 50-51 0.0 0.0 0.0 0.18587794344140762 0.0 52-53 0.0 0.0 0.0 0.2406271204086981 0.0 54-55 0.0 0.0 0.0 0.3151219021838639 0.0 56-57 0.0 0.0 0.0 0.40451564031406284 0.0 58-59 0.0 0.0 0.0 0.5167963294715717 0.0 60-61 0.0 0.0 0.0 0.6369752605522307 0.0 62-63 0.0 0.0 0.0 0.7883881483289834 0.0 64-65 0.0 0.0 0.0 0.9363006788897944 0.0 66-67 0.0 0.0 0.0 1.1110492814394906 0.0 68-69 0.0 0.0 0.0 1.3391110169704499 0.0 70-71 0.0 0.0 0.0 1.5588257468326254 0.0 72-73 0.0 0.0 0.0 1.8060945706767 0.0 74-75 0.0 0.0 0.0 2.0989577910771406 0.0 76-77 0.0 0.0 0.0 2.4092330448081625 0.0 78-79 0.0 0.0 0.0 2.722649644758619 0.0 80-81 0.0 0.0 0.0 3.1146896529440697 0.0 82-83 0.0 0.0 0.0 3.538502134320374 0.0 84-85 0.0 0.0 0.0 3.9572884617455832 0.0 86-87 0.0 0.0 0.0 4.40829602610728 0.0 88-89 0.0 0.0 0.0 4.9704174938878385 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 55 0.0025756701 34.55316 2 CCGATCG 80 4.4895572E-4 29.688791 9 GAGTACT 4395 0.0 24.804832 12-13 ACTATCC 135 1.3406612E-5 24.6307 8 GTGCTAT 100 0.0016478492 23.761698 1 CGGGCCG 100 0.0016504355 23.755297 5 GTACTTT 4610 0.0 23.491322 14-15 AGTACTT 4490 0.0 23.32785 12-13 GTACATG 3930 0.0 21.645515 1 TACATGG 3990 0.0 21.07613 2 ACTTTTT 5240 0.0 20.757626 16-17 TACTTTT 5035 0.0 20.706589 14-15 ACACGCT 115 0.003719967 20.653072 9 ACTCCGG 115 0.003719967 20.653072 8 GTAAGGT 185 6.5295135E-6 20.545122 4 AGAGTAC 7170 0.0 20.537851 10-11 GGTAAGG 210 8.5729516E-7 20.361683 3 GGCCGAT 140 4.8460008E-4 20.358027 7 TAGCGCG 70 4.8531248E-4 20.356201 28-29 TAAGGTG 260 1.4821126E-8 20.100637 5 >>END_MODULE