FastQCFastQC Report
Thu 26 May 2016
SRR937561_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937561_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502579
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56741.128976737985471No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41400.8237510918681442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28800.5730442378213176No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27660.5503612367408905No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26030.5179285246697535No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21170.4212273095374061No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16970.3376583581884639No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT11740.23359511638966213No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC10520.20932032575973128No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10010.19917266738164546No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT8480.16872969224738796No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC7970.15858203386930214No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7780.15480153368923094No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC7060.140475427743698No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6670.13271545368986767No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6180.12296574269915773No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT6120.12177190053703No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG6040.12018011098752633No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA5740.11421090017688762No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC5660.11261911062738394No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA5590.11122629477156826No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5280.10505811026724156No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG36500.028.1397611
TACATGG36300.028.027222
CCGGACA908.9002016E-426.4093074
ACATGGG36900.026.4093073
GAGTACT36100.025.06518412-13
CATGGGA25700.023.860864
CCCCCGA1000.001643972423.7707482
AGTACTT36900.023.17017212-13
GTACTTT39200.023.14357614-15
CCCGGAC1050.002184664822.6365493
CATGGGG9800.022.3131714
CTAGACT1302.92298E-421.940044
GTATCAA76600.021.6648731
GTCGGCG550.002877517121.59038712-13
ATGGGGA5400.021.1232345
ACTTTTT44950.020.65856716-17
ATGGGAT11100.020.5523365
GGTATCA60500.020.3565371
AGAGTAC57950.020.2044310-11
TACTTTT42900.019.87422814-15
Produced by FastQC (version 0.11.5)