##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937561_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 502579 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.942046921976445 33.0 31.0 34.0 30.0 34.0 2 32.05451282285969 33.0 31.0 34.0 30.0 34.0 3 32.0571731011443 34.0 31.0 34.0 30.0 34.0 4 35.64344908959586 37.0 35.0 37.0 35.0 37.0 5 35.645017002302126 37.0 35.0 37.0 35.0 37.0 6 35.72129555751434 37.0 35.0 37.0 35.0 37.0 7 35.68346070965958 37.0 35.0 37.0 35.0 37.0 8 35.67725869962732 37.0 35.0 37.0 35.0 37.0 9 37.31767741986832 39.0 37.0 39.0 35.0 39.0 10-11 37.22353799104221 39.0 37.0 39.0 34.0 39.0 12-13 37.12413670288652 39.0 37.0 39.0 34.0 39.0 14-15 38.248235600771224 40.0 38.0 41.0 33.5 41.0 16-17 38.22528000573044 40.0 38.0 41.0 33.5 41.0 18-19 38.24540619484698 40.0 38.0 41.0 33.5 41.0 20-21 38.21576906317216 40.0 38.0 41.0 34.0 41.0 22-23 38.03250135799546 40.0 38.0 41.0 33.5 41.0 24-25 37.86350106152466 40.0 38.0 41.0 33.0 41.0 26-27 37.79314296856813 40.0 37.5 41.0 33.0 41.0 28-29 37.81168333734597 40.0 38.0 41.0 33.0 41.0 30-31 37.59753889438277 40.0 37.0 41.0 33.0 41.0 32-33 37.280395718882005 39.5 36.0 41.0 32.5 41.0 34-35 37.26304819739782 40.0 36.0 41.0 33.0 41.0 36-37 37.15014952873081 40.0 36.0 41.0 32.0 41.0 38-39 36.99347366284704 40.0 36.0 41.0 32.0 41.0 40-41 36.76236870223387 39.5 35.5 41.0 31.0 41.0 42-43 36.44712771524477 39.0 35.0 41.0 30.5 41.0 44-45 36.104424379052844 39.0 35.0 41.0 30.0 41.0 46-47 35.60243265237903 39.0 35.0 41.0 27.5 41.0 48-49 35.12208130463071 39.0 35.0 41.0 24.5 41.0 50-51 34.142853163383265 38.0 34.0 39.5 23.5 40.5 52-53 34.22587095760069 38.0 34.5 39.5 23.0 40.5 54-55 34.58583725145698 39.0 35.0 40.0 23.0 41.0 56-57 34.36082884481843 38.5 34.5 40.0 21.5 41.0 58-59 34.12477540844325 38.0 34.0 40.0 20.0 41.0 60-61 33.80227785084534 38.0 34.0 40.0 18.5 41.0 62-63 33.49919415654057 38.0 34.0 40.0 16.5 41.0 64-65 33.135287188680785 37.0 33.0 40.0 12.0 41.0 66-67 32.70701123604448 36.5 33.0 39.5 8.0 41.0 68-69 32.23096965850145 36.0 32.0 39.0 6.0 41.0 70-71 31.790065840395243 35.5 31.5 39.0 2.0 40.0 72-73 31.340809106628015 35.0 31.0 38.0 2.0 40.0 74-75 30.898127458568702 35.0 31.0 37.0 2.0 39.5 76-77 30.424301453104885 35.0 30.5 37.0 2.0 39.0 78-79 29.914393558027694 34.5 30.0 36.0 2.0 39.0 80-81 29.37168087007217 34.0 29.0 36.0 2.0 37.5 82-83 28.851968546238503 34.0 29.0 35.0 2.0 37.0 84-85 28.392660656334627 34.0 28.5 35.0 2.0 37.0 86-87 27.97098068164408 34.0 27.0 35.0 2.0 36.0 88-89 27.504341605996274 34.0 26.0 35.0 2.0 36.0 90-91 27.016295945513043 33.0 25.5 35.0 2.0 35.5 92-93 26.491160593657913 33.0 25.0 35.0 2.0 35.0 94-95 25.901116043447892 32.5 23.5 35.0 2.0 35.0 96-97 25.220006207979242 32.0 19.0 35.0 2.0 35.0 98-99 24.357952680076167 32.0 5.0 34.0 2.0 35.0 100-101 22.109874268522958 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07610000450804932 1101 2 0.2788962507430881 1101 3 0.10596880579854329 1101 4 0.14516864157238984 1101 5 0.1248011031546099 1101 6 0.02237246191478448 1101 7 0.1328300082927072 1101 8 0.04822857964349936 1101 9 0.2062321010609054 1101 10-11 0.11352797710910068 1101 12-13 0.2949969723808863 1101 14-15 0.3990120265087711 1101 16-17 0.47442918078304075 1101 18-19 0.47873479202027625 1101 20-21 0.5827971683314743 1101 22-23 0.23840254002518435 1101 24-25 0.448369546264459 1101 26-27 0.45656705444352497 1101 28-29 0.4298321430818035 1101 30-31 0.4105936551349103 1101 32-33 0.5228932185220287 1101 34-35 0.661083641598367 1101 36-37 0.6872240861542096 1101 38-39 0.5952804051456368 1101 40-41 0.5476262823103752 1101 42-43 0.6118151865629002 1101 44-45 0.5959781006284928 1101 46-47 0.7577929231249954 1101 48-49 0.7016014128224768 1101 50-51 0.7431058821539409 1101 52-53 0.9361783894741507 1101 54-55 0.8778487076281394 1101 56-57 0.9630291517629956 1101 58-59 0.8773525590484184 1101 60-61 0.9660839648942883 1101 62-63 1.090867047032397 1101 64-65 0.9168502002030934 1101 66-67 1.0548753773436133 1101 68-69 0.9995066796549885 1101 70-71 1.0425993136766074 1101 72-73 1.005249999929589 1101 74-75 1.0348716189465144 1101 76-77 1.1760411635422336 1101 78-79 1.150702197697484 1101 80-81 1.244574900838316 1101 82-83 1.296942269978878 1101 84-85 1.3665054378934443 1101 86-87 1.5800560893994984 1101 88-89 1.3808009958413159 1101 90-91 1.5652951908608301 1101 92-93 1.45432804590153 1101 94-95 1.521061828963969 1101 96-97 1.5789325012183006 1101 98-99 1.5668317858535552 1101 100-101 2.002087370375161 1102 1 0.11696674953438446 1102 2 0.06609097452525248 1102 3 0.28154453171383054 1102 4 0.0741094800208657 1102 5 0.038561932025793055 1102 6 0.060294189310198476 1102 7 0.19781847200782465 1102 8 0.0744551261586679 1102 9 0.17530832760741788 1102 10-11 0.11298925437173324 1102 12-13 0.05405948598500032 1102 14-15 0.04596797096046856 1102 16-17 0.08592737415729346 1102 18-19 0.19842048267371126 1102 20-21 0.29836135796188046 1102 22-23 0.33442361007002575 1102 24-25 0.3783540919960373 1102 26-27 0.2667625182892408 1102 28-29 0.08287728695886898 1102 30-31 0.21708771285987183 1102 32-33 0.10655196215530793 1102 34-35 0.17487059035985197 1102 36-37 0.40362084728781156 1102 38-39 0.6488664484175857 1102 40-41 0.4453995616626045 1102 42-43 0.4454271320593932 1102 44-45 0.5224277545399545 1102 46-47 0.3513380452354866 1102 48-49 0.5145394651493191 1102 50-51 0.546962919287715 1102 52-53 0.480096808350126 1102 54-55 0.6256513718569465 1102 56-57 0.6861844345108068 1102 58-59 0.6581109065300197 1102 60-61 0.7447875476725727 1102 62-63 0.8492907116530048 1102 64-65 0.9401393473886728 1102 66-67 0.9475563664167979 1102 68-69 1.050389314281258 1102 70-71 1.0269273794987335 1102 72-73 1.0202558769049013 1102 74-75 1.0850664297949741 1102 76-77 0.9047360396904196 1102 78-79 0.8874605016459434 1102 80-81 1.026111403385002 1102 82-83 1.001119275768783 1102 84-85 1.0927733409015872 1102 86-87 1.0603399255407204 1102 88-89 1.132734956052019 1102 90-91 1.1885832497176523 1102 92-93 1.2648913648689195 1102 94-95 1.3684084834413532 1102 96-97 1.6972610459050834 1102 98-99 1.9251836207758473 1102 100-101 2.5234360273774676 1103 1 0.19633443135318984 1103 2 0.04429321250533036 1103 3 0.24046844312369942 1103 4 0.1886973391838822 1103 5 0.11190428286128196 1103 6 0.0763467094287904 1103 7 0.034996540349560235 1103 8 0.2140597223521894 1103 9 0.1261028241985258 1103 10-11 0.05187518296951055 1103 12-13 0.17622546267612194 1103 14-15 0.4945839210089673 1103 16-17 0.2062755043918827 1103 18-19 0.10777887373271255 1103 20-21 0.16822445588375246 1103 22-23 0.23508830928167868 1103 24-25 0.20904980594993816 1103 26-27 0.1333478908077126 1103 28-29 0.13606552048485554 1103 30-31 0.03169811577826209 1103 32-33 0.28363521009562476 1103 34-35 0.273660742154469 1103 36-37 0.3445992462712937 1103 38-39 0.6192548841542234 1103 40-41 0.5762763088036493 1103 42-43 0.8510931292448376 1103 44-45 0.7662610127274334 1103 46-47 1.0051725330823587 1103 48-49 0.9892938748445061 1103 50-51 0.9521712099245434 1103 52-53 0.8120419928673428 1103 54-55 0.8525727146449995 1103 56-57 0.9140526150248078 1103 58-59 0.9614159576975112 1103 60-61 1.2837253952221346 1103 62-63 1.2641163049636575 1103 64-65 1.270099998288373 1103 66-67 1.1980940008253995 1103 68-69 1.4382962973802655 1103 70-71 1.2677766642468562 1103 72-73 1.4837595863773778 1103 74-75 1.0932757724737208 1103 76-77 1.3165339383391625 1103 78-79 1.412833156753809 1103 80-81 1.4008562490627483 1103 82-83 1.541395402614981 1103 84-85 1.414532846851774 1103 86-87 1.490299210152216 1103 88-89 1.5718695491030736 1103 90-91 1.4658502634215225 1103 92-93 1.5348740130087215 1103 94-95 1.6727949517229739 1103 96-97 1.3450490253014529 1103 98-99 1.1973625772049168 1103 100-101 1.7041358401959847 1104 1 0.03765732590518667 1104 2 0.006603897787123003 1104 3 -0.13096131213843876 1104 4 -0.004522056745308589 1104 5 -0.05547399931729302 1104 6 -0.12562713574155993 1104 7 -0.12094196344264674 1104 8 -0.15887152848635822 1104 9 -0.20271878786710573 1104 10-11 -0.14429886424176885 1104 12-13 -0.13648516104633046 1104 14-15 0.030628693594543677 1104 16-17 0.2046043260039525 1104 18-19 0.12362251299495597 1104 20-21 0.27144290474609534 1104 22-23 0.18713515752211407 1104 24-25 -0.09522501706694442 1104 26-27 -0.053881307263424105 1104 28-29 0.035079584427649024 1104 30-31 0.19254854235295937 1104 32-33 0.23346039733863222 1104 34-35 0.3293670172616032 1104 36-37 0.2888692944927769 1104 38-39 0.3528903581217193 1104 40-41 0.29400204613465775 1104 42-43 0.4819461997145069 1104 44-45 0.39322518992364763 1104 46-47 0.48198320652581117 1104 48-49 0.6446069199012143 1104 50-51 0.7415247973348684 1104 52-53 0.7851926475273032 1104 54-55 0.6383442189817714 1104 56-57 0.748551695580737 1104 58-59 0.7169651775511952 1104 60-61 0.9602971315902948 1104 62-63 0.7373892890923983 1104 64-65 0.7566891370099924 1104 66-67 0.9030629449247769 1104 68-69 0.8525097978182821 1104 70-71 1.1575124325942987 1104 72-73 1.1919248811124739 1104 74-75 1.1237644195097936 1104 76-77 1.3638507795555093 1104 78-79 1.444039009626973 1104 80-81 1.5429567249638438 1104 82-83 1.394589188277969 1104 84-85 1.2580675371214483 1104 86-87 1.212911220538384 1104 88-89 1.329470747142647 1104 90-91 1.5518103919069262 1104 92-93 1.4371781477447634 1104 94-95 1.3072350379060573 1104 96-97 1.7618857036223048 1104 98-99 1.9669688341599425 1104 100-101 2.2329414705084396 1105 1 -0.030175299135677847 1105 2 -0.10875557111925716 1105 3 -0.19261419015287018 1105 4 -0.11798925316445263 1105 5 -0.4116361438089555 1105 6 -0.3237813842390551 1105 7 -0.19611736012834768 1105 8 -0.0357795175224922 1105 9 -0.27048854671237876 1105 10-11 -0.14912384475324814 1105 12-13 -0.233849181465267 1105 14-15 -0.3626276496045904 1105 16-17 -0.25988256010765554 1105 18-19 -0.672074924578915 1105 20-21 -0.31851862114221063 1105 22-23 -0.24439098224201672 1105 24-25 -0.49140778167076604 1105 26-27 -0.4320969131678183 1105 28-29 -0.2877791929505804 1105 30-31 -0.22958853109989974 1105 32-33 -0.2245764171055029 1105 34-35 -0.11928073087126734 1105 36-37 -0.12451680453405345 1105 38-39 0.014814818101100968 1105 40-41 0.03851697056948211 1105 42-43 0.2869164011377947 1105 44-45 0.5387918658106869 1105 46-47 0.43744879357034705 1105 48-49 0.37062975290344724 1105 50-51 0.1469520360000942 1105 52-53 -0.004105011335568065 1105 54-55 0.31661388981437 1105 56-57 0.04197652018404341 1105 58-59 0.4198626805059007 1105 60-61 0.20090177865947112 1105 62-63 0.37831643974494256 1105 64-65 0.5995425078954 1105 66-67 0.7922235554812715 1105 68-69 0.6204580966590321 1105 70-71 0.8634495161057565 1105 72-73 0.9343997975004363 1105 74-75 0.7286975298776888 1105 76-77 0.771182863947093 1105 78-79 0.9265119142966753 1105 80-81 0.6904251032674651 1105 82-83 0.5428936869829251 1105 84-85 0.7197443042902094 1105 86-87 0.8917969800877579 1105 88-89 1.2466916841004263 1105 90-91 1.1159024783102254 1105 92-93 1.0960284507598281 1105 94-95 1.0585112216765218 1105 96-97 1.172555982999679 1105 98-99 1.1963864417240018 1105 100-101 1.4464193541808292 1106 1 -0.14500783502084502 1106 2 0.016332711863761062 1106 3 0.009969144009410513 1106 4 -0.22123806693958414 1106 5 -0.11181718609525859 1106 6 0.02581425483026578 1106 7 -0.11493680526358219 1106 8 -0.36065094765707784 1106 9 -0.06683586937548824 1106 10-11 -0.05785167453370832 1106 12-13 -0.11988704118733295 1106 14-15 -0.18390310245899855 1106 16-17 -0.16675023013321777 1106 18-19 0.2839228472083306 1106 20-21 0.07901840797376991 1106 22-23 -0.01786430834692254 1106 24-25 0.0873829155409922 1106 26-27 -5.025473629274302E-4 1106 28-29 0.08953232909778563 1106 30-31 0.11651309131750764 1106 32-33 0.3415761358891558 1106 34-35 -0.16328295908402168 1106 36-37 0.0044807981477603676 1106 38-39 -0.14979937282888045 1106 40-41 -0.06565694939390454 1106 42-43 0.2847603068764357 1106 44-45 0.2468313370080537 1106 46-47 0.3216637962064013 1106 48-49 0.3214996511417709 1106 50-51 0.19858639026602276 1106 52-53 0.3225818153512563 1106 54-55 0.3525040309676726 1106 56-57 0.17015843783965323 1106 58-59 0.1873228163870948 1106 60-61 0.060166269502914815 1106 62-63 0.19283513584259282 1106 64-65 0.3953228299915068 1106 66-67 0.5187328214642193 1106 68-69 0.09528376885312895 1106 70-71 0.15104563264923954 1106 72-73 0.4043487674494095 1106 74-75 0.5711658723460324 1106 76-77 0.5618626677847907 1106 78-79 0.3448737800269619 1106 80-81 0.4988584906482174 1106 82-83 -0.23358126054465345 1106 84-85 0.2059249088918662 1106 86-87 0.5708323828073709 1106 88-89 0.6362011788730797 1106 90-91 0.9061719527902277 1106 92-93 0.6514676772516843 1106 94-95 0.9321148584380516 1106 96-97 1.1423681764960811 1106 98-99 1.1925094510049021 1106 100-101 1.5494617598548182 1107 1 -0.009942899955909468 1107 2 0.19085008638113266 1107 3 0.008705542745808259 1107 4 0.06405501835350691 1107 5 -0.3049375792156539 1107 6 0.09622492524093218 1107 7 0.1874148970881251 1107 8 0.10674111973498412 1107 9 0.09666009412047316 1107 10-11 0.30167798499594767 1107 12-13 -0.04115766245795527 1107 14-15 0.19159707304118 1107 16-17 0.07284260945962728 1107 18-19 0.16230122558670956 1107 20-21 0.1599239888793491 1107 22-23 0.1518443613617464 1107 24-25 0.37555420371227655 1107 26-27 0.29911135225097496 1107 28-29 0.14390228346774592 1107 30-31 0.3156004904049112 1107 32-33 0.241365535678554 1107 34-35 0.2086019128008516 1107 36-37 0.4016727953397563 1107 38-39 0.2890563660268626 1107 40-41 0.4557891720522136 1107 42-43 0.6765819986981327 1107 44-45 0.7350377252144398 1107 46-47 1.3475676221102333 1107 48-49 1.461969991612115 1107 50-51 0.9225948142744542 1107 52-53 0.9489419417113822 1107 54-55 1.0501172285808664 1107 56-57 1.1795652472464582 1107 58-59 1.4266348556991346 1107 60-61 1.2646590739957162 1107 62-63 1.0585073015147586 1107 64-65 1.0610651957338746 1107 66-67 0.8721199748513797 1107 68-69 0.7643957379650992 1107 70-71 1.002712884316491 1107 72-73 0.8293048924055348 1107 74-75 1.0358903370704944 1107 76-77 1.033712439634563 1107 78-79 1.0062637414169266 1107 80-81 0.9068612986510303 1107 82-83 1.1505886236252287 1107 84-85 1.0493436523106077 1107 86-87 0.7685859805609674 1107 88-89 1.010964253636157 1107 90-91 1.306101752720025 1107 92-93 0.9080138337978418 1107 94-95 1.0087031350263267 1107 96-97 1.5095847437126473 1107 98-99 2.0036712621667156 1107 100-101 1.9827014930945488 1108 1 0.060664044120422034 1108 2 0.07359591198410698 1108 3 0.07872939644313348 1108 4 0.14261319487086155 1108 5 0.4537160120910002 1108 6 0.34027475296423404 1108 7 0.44826832937012995 1108 8 0.3311779074370804 1108 9 0.45934230986391356 1108 10-11 0.11731540879663527 1108 12-13 0.16011453473260673 1108 14-15 0.32263338366932715 1108 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-1.2594076687502422 2316 94-95 -1.1704066792100676 2316 96-97 -0.8345546611862495 2316 98-99 -0.9324827736708627 2316 100-101 -1.1766524441074893 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2119.0 3 374.0 4 312.0 5 344.0 6 414.0 7 462.0 8 540.0 9 698.0 10 902.0 11 1205.0 12 1855.0 13 2641.0 14 2876.0 15 3983.0 16 5490.0 17 8136.0 18 6708.0 19 3948.0 20 3522.0 21 3521.0 22 3646.0 23 3931.0 24 4383.0 25 4838.0 26 5468.0 27 6267.0 28 7655.0 29 9008.0 30 11059.0 31 13785.0 32 17907.0 33 23457.0 34 32726.0 35 48339.0 36 68099.0 37 97416.0 38 82691.0 39 11851.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.68078736920255 19.38706065728524 15.655179708658226 20.276972264853978 2 14.684319750967795 21.011294249112026 41.4201620305703 22.884223969349883 3 19.64429298542096 26.62415527564899 30.724722116364955 23.00682962256509 4 11.609656061171279 17.040143153241395 39.24325877102863 32.1069420145587 5 11.978009263876864 38.97690414161523 34.37528675329442 14.669799841213479 6 28.81293751956439 37.39275723807644 18.113085660623387 15.681219581735784 7 25.9529140159452 35.38027843291274 21.115725299196757 17.551082251945303 8 25.815565786613977 33.36616665636471 20.289996670142745 20.52827088687857 9 26.338352821069087 17.325208569286477 21.80705546427603 34.529383145368406 10-11 24.17139575598298 27.494287206932714 26.850127416222335 21.48418962086196 12-13 26.177302164585026 25.611730551434814 27.90302834537653 20.307938938603627 14-15 22.81436140782869 25.933277174373572 28.764087508286767 22.48827390951097 16-17 19.835746393867705 29.97428926619233 28.68775564933311 21.502208690606857 18-19 19.506490865335728 27.793983064533816 32.809026400106866 19.890499670023587 20-21 21.34106166551207 25.7337513144976 32.59944080898294 20.32574621100739 22-23 20.498651955287777 26.06415795794898 32.97900439048943 20.458185696273816 24-25 20.58172148255055 26.215142460166373 32.94973178457447 20.253404272708607 26-27 20.04972183484336 26.08990800264359 33.758115434801894 20.10225472771115 28-29 19.45010508181227 27.341011417428028 33.26557184844292 19.943311652316783 30-31 19.753698117195274 26.436428672545905 33.148668398668896 20.661204811589926 32-33 19.74142039325316 26.64697941612214 33.15583568789393 20.45576450273076 34-35 20.70079282559133 26.475470057949142 32.65946979477052 20.164267321689007 36-37 20.19982736382973 26.167835848120042 33.460119250527775 20.172217537522453 38-39 19.945916474543566 26.95614210830061 32.934434938794716 20.163506478361107 40-41 19.99156793542992 27.17177989321378 32.85166244232857 19.98498972902773 42-43 19.749057245132214 27.153134491719804 31.993556471091345 21.104251792056637 44-45 19.902475200153106 26.899460942234697 31.977050173838155 21.221013683774043 46-47 20.703771743670153 27.480131536059282 31.48691766436774 20.329179055902824 48-49 20.53264082383376 27.414304441664218 31.08467124905918 20.968383485442846 50-51 19.852683129147525 27.961392132465544 31.540127297090354 20.64579744129658 52-53 20.422592093624818 28.49859046221073 30.219652556663217 20.859164887501233 54-55 19.9598858381258 27.735593443368156 30.77475957920005 21.529761139305997 56-57 20.661301156215682 27.808472005469152 30.2103949789027 21.319831859412464 58-59 21.153757995045016 27.512920630554678 30.330445251008037 21.002876123392273 60-61 20.62351123735486 27.60900981711267 31.402102955100414 20.365375990432053 62-63 20.104975784402942 27.912375321321388 31.188732147289038 20.793916746986632 64-65 19.906438949620743 28.37327550380752 30.860396906944228 20.85988863962751 66-67 20.222278922073805 27.757057968732248 30.266591184989245 21.754071924204702 68-69 20.018857788156872 27.680482089586455 30.687860747394346 21.61279937486233 70-71 20.706904095335577 27.69681101816794 30.240461807863774 21.355823078632717 72-73 20.414832799119743 27.249178746272463 31.014288220191677 21.321700234416113 74-75 19.912829667016503 28.45484455155518 30.262437918795843 21.36988786263247 76-77 19.940528702427198 27.74586237867247 30.367842834685543 21.94576608421479 78-79 20.197701392343355 27.686967478758962 30.515217883243334 21.60011324565435 80-81 20.53160875142915 27.83260683858659 30.009698779628373 21.626085630355885 82-83 20.297959378962226 27.597226541629787 30.64649569787007 21.45831838153792 84-85 20.270046685564154 27.84927459962444 30.179865006558305 21.7008137082531 86-87 20.07846037462038 27.966159976637307 30.27771105412191 21.6776685946204 88-89 20.061581338601673 27.758251879436934 29.652839938358856 22.527326843602538 90-91 20.104005829270555 27.57520087957393 29.924851452283814 22.395941838871707 92-93 20.69843681006789 27.836402799249253 30.050465029817524 21.41469536086533 94-95 20.237819950562155 27.69236902958297 29.902519735268317 22.167291284586558 96-97 19.596092283036395 27.833413730829182 30.507361354186184 22.06313263194824 98-99 20.30358242814113 28.324027246468102 29.156790785478915 22.21559953991185 100-101 20.306169738828842 27.298341082258197 29.814175300115114 22.581313878797847 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 873.0 1 557.5 2 343.0 3 418.0 4 311.0 5 287.0 6 534.5 7 1190.0 8 2077.0 9 2390.0 10 1929.5 11 1476.5 12 1424.0 13 1488.5 14 1514.0 15 1479.0 16 1468.0 17 1507.5 18 1567.0 19 1675.5 20 1758.5 21 1898.0 22 2172.5 23 2567.5 24 3041.5 25 3625.0 26 4417.5 27 5214.0 28 5844.0 29 6604.0 30 7610.5 31 8495.0 32 9317.0 33 10373.0 34 11613.0 35 12542.0 36 13757.0 37 14865.5 38 15104.5 39 15189.5 40 15298.0 41 15773.0 42 15991.0 43 16277.0 44 17013.0 45 17697.0 46 17903.0 47 20201.5 48 25769.0 49 24781.0 50 18365.0 51 14911.5 52 13541.5 53 12797.5 54 11868.0 55 10747.0 56 9502.5 57 8345.5 58 7319.5 59 6279.0 60 5196.0 61 4200.0 62 3317.5 63 2462.0 64 1736.0 65 1304.5 66 971.0 67 718.5 68 546.5 69 395.5 70 272.0 71 209.0 72 170.0 73 137.5 74 119.0 75 101.5 76 72.5 77 50.5 78 39.5 79 27.5 80 19.5 81 15.5 82 7.5 83 6.0 84 5.0 85 4.5 86 4.0 87 1.5 88 0.0 89 1.0 90 1.0 91 1.0 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30204206701831954 2 0.2863231452169709 3 0.27458369728938137 4 0.25826785440696887 5 0.25408940683952175 6 0.20494290449859626 7 0.19618806197632613 8 0.21011622053448312 9 0.21230493116505067 10-11 0.2134987733271784 12-13 0.20912135206604335 14-15 0.20424649657068839 16-17 0.20643520720125594 18-19 0.2045449571112203 20-21 0.19131320648097117 22-23 0.18434912720189264 24-25 0.1850455351298005 26-27 0.196685496210546 28-29 0.21190698377767475 30-31 0.20693264143547582 32-33 0.21290185224611455 34-35 0.20265470702118474 36-37 0.1882291142288078 38-39 0.18852757476933976 40-41 0.1835532324271408 42-43 0.1970834435979219 44-45 0.1916116670215031 46-47 0.1925070486430989 48-49 0.2032516281022486 50-51 0.21389672071455432 52-53 0.23349562954281816 54-55 0.2010629174716811 56-57 0.17032148179689163 58-59 0.17151532395901936 60-61 0.1798722190939136 62-63 0.1869357852198361 64-65 0.17400249513011884 66-67 0.18146400864341725 68-69 0.18494604828295652 70-71 0.17877786377862984 72-73 0.18046914017497748 74-75 0.18255836395870104 76-77 0.19748139098529782 78-79 0.2018588122464329 80-81 0.1929049960304748 82-83 0.1924075617962549 84-85 0.1840506666613607 86-87 0.18395117981451672 88-89 0.19220858810256697 90-91 0.1935019171115387 92-93 0.18793065368827588 94-95 0.1868362983729921 96-97 0.18007119278760156 98-99 0.18623937729192824 100-101 0.17837991639125392 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 502579.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.6483015887619 #Duplication Level Percentage of deduplicated Percentage of total 1 83.02987942666626 52.01680927200325 2 10.135520970204473 12.699463490011809 3 2.941931161637286 5.52920972002888 4 1.2358175969995624 3.0968749410211047 5 0.6820578764360117 2.136488377197688 6 0.409893518365471 1.5407479654703247 7 0.277590344210764 1.217339452157092 8 0.20104812466717245 1.0076258838403218 9 0.16272830799877883 0.9175186904882788 >10 0.8581836788065162 9.469361659716649 >50 0.03965351616855821 1.6459483997671078 >100 0.018716459166857234 2.1720079327578836 >500 0.0038067374576658724 1.561484703786136 >1k 0.002855053093249405 3.8614799280341887 >5k 3.172281214721561E-4 1.1276395837192996 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5674 1.128976737985471 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4140 0.8237510918681442 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2880 0.5730442378213176 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2766 0.5503612367408905 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2603 0.5179285246697535 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2117 0.4212273095374061 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1697 0.3376583581884639 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1174 0.23359511638966213 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1052 0.20932032575973128 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1001 0.19917266738164546 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 848 0.16872969224738796 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 797 0.15858203386930214 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 778 0.15480153368923094 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 706 0.140475427743698 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 667 0.13271545368986767 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 618 0.12296574269915773 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 612 0.12177190053703 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 604 0.12018011098752633 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 574 0.11421090017688762 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 566 0.11261911062738394 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 559 0.11122629477156826 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 528 0.10505811026724156 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 9.948684684397876E-5 0.0 22-23 0.0 0.0 0.0 1.9897369368795752E-4 0.0 24-25 0.0 0.0 0.0 1.9897369368795752E-4 0.0 26-27 0.0 0.0 0.0 8.953816215958088E-4 0.0 28-29 0.0 0.0 0.0 0.0014923027026596813 0.0 30-31 0.0 0.0 0.0 0.005173316035886895 0.0 32-33 0.0 0.0 0.0 0.008854329369114108 0.0 34-35 0.0 0.0 0.0 0.013729184864469068 0.0 36-37 0.0 0.0 0.0 0.018405066666136068 0.0 38-39 0.0 0.0 0.0 0.024374277476774793 0.0 40-41 0.0 0.0 0.0 0.03412398846748471 0.0 42-43 0.0 0.0 0.0 0.05233008143993283 0.0 44-45 0.0 0.0 0.0 0.07998742486255891 0.0 46-47 0.0 0.0 0.0 0.11222116324000804 0.0 48-49 0.0 0.0 0.0 0.1677348237789482 0.0 50-51 0.0 0.0 0.0 0.2215572079215407 0.0 52-53 0.0 0.0 0.0 0.280751881793708 0.0 54-55 0.0 0.0 0.0 0.36233109620577064 0.0 56-57 0.0 0.0 0.0 0.4579379560228342 0.0 58-59 0.0 0.0 0.0 0.5774216590824527 0.0 60-61 0.0 0.0 0.0 0.7150119682676753 0.0 62-63 0.0 0.0 0.0 0.8903077924067659 0.0 64-65 0.0 0.0 0.0 1.049088799969756 0.0 66-67 0.0 0.0 0.0 1.2349302298743083 0.0 68-69 0.0 0.0 0.0 1.4740966096872332 0.0 70-71 0.0 0.0 0.0 1.7152527264370376 0.0 72-73 0.0 0.0 0.0 1.9813800417446807 0.0 74-75 0.0 0.0 0.0 2.2888938853394194 0.0 76-77 0.0 0.0 0.0 2.6013820712763565 0.0 78-79 0.0 0.0 0.0 2.9271020078435432 0.0 80-81 0.0 0.0 0.0 3.320870947652011 0.0 82-83 0.0 0.0 0.0 3.7567228236754815 0.0 84-85 0.0 0.0 0.0 4.206403371410266 0.0 86-87 0.0 0.0 0.0 4.685233565270336 0.0 88-89 0.0 0.0 0.0 5.298768949757153 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 3650 0.0 28.139761 1 TACATGG 3630 0.0 28.02722 2 CCGGACA 90 8.9002016E-4 26.409307 4 ACATGGG 3690 0.0 26.409307 3 GAGTACT 3610 0.0 25.065184 12-13 CATGGGA 2570 0.0 23.86086 4 CCCCCGA 100 0.0016439724 23.770748 2 AGTACTT 3690 0.0 23.170172 12-13 GTACTTT 3920 0.0 23.143576 14-15 CCCGGAC 105 0.0021846648 22.636549 3 CATGGGG 980 0.0 22.313171 4 CTAGACT 130 2.92298E-4 21.94004 4 GTATCAA 7660 0.0 21.664873 1 GTCGGCG 55 0.0028775171 21.590387 12-13 ATGGGGA 540 0.0 21.123234 5 ACTTTTT 4495 0.0 20.658567 16-17 ATGGGAT 1110 0.0 20.552336 5 GGTATCA 6050 0.0 20.356537 1 AGAGTAC 5795 0.0 20.20443 10-11 TACTTTT 4290 0.0 19.874228 14-15 >>END_MODULE