FastQCFastQC Report
Thu 26 May 2016
SRR937561_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937561_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502579
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55751.1092783423103632No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54281.0800292093382335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44200.8794637261007722No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32060.6379096619635918No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15770.313781514945909No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC12680.2522986435963301No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12660.2519006962089542No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT11370.22623308972320771No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11200.22285053693051243No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA11140.22165669476838468No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11060.220064905218881No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT10440.20772853621022763No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG9960.19817779891320567No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA8620.17151532395901936No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC8430.1677348237789482No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8140.16196458666199742No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC6470.12873597981610851No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA5190.10326734702404995No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG650.005839130429.2326689
CGGTGTT700.008388570527.141924
TAAGGTG1457.875133E-726.205995
CTGGTCG950.001225130325.0016219
GTACATG36050.024.384731
AGTACTT37800.024.31705512-13
TACATGG35650.024.1155762
CTATTAC1000.001648862623.7586331
GAGTACT36650.023.20068712-13
GTAATAC1050.002194955522.6182653
ACATGGG36550.022.4821223
GTACTTT38750.021.8798914-15
GTTGTGC2205.619586E-821.5923128
TACTTTT42900.021.14728714-15
ACTGATC1353.790182E-421.112488
AGTACAC2257.1340764E-821.1103825
AGAGTAC63100.021.07902510-11
TATGTAC3201.8189894E-1120.7805333
CATGGGA26250.020.627864
GCGTAGA1404.8346454E-420.3645441
Produced by FastQC (version 0.11.5)