##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937561_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 502579 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17510480939315 33.0 31.0 34.0 31.0 34.0 2 32.37358902779464 34.0 31.0 34.0 31.0 34.0 3 32.41434480947274 34.0 31.0 34.0 31.0 34.0 4 36.04441291816809 37.0 35.0 37.0 35.0 37.0 5 35.97576301437187 37.0 35.0 37.0 35.0 37.0 6 36.04835657677698 37.0 35.0 37.0 35.0 37.0 7 36.0080445064358 37.0 35.0 37.0 35.0 37.0 8 35.9876934770454 37.0 35.0 37.0 35.0 37.0 9 37.647892172175915 39.0 37.0 39.0 35.0 39.0 10-11 37.55720991127763 39.0 37.0 39.0 35.0 39.0 12-13 37.45478223324094 39.0 37.0 39.0 35.0 39.0 14-15 38.542088905425814 40.0 38.0 41.0 34.0 41.0 16-17 38.543016122838395 40.0 38.0 41.0 34.0 41.0 18-19 38.601792951953826 40.0 38.0 41.0 34.0 41.0 20-21 38.539490308986245 40.0 38.0 41.0 34.0 41.0 22-23 38.48144470819513 40.0 38.0 41.0 34.0 41.0 24-25 38.46479061003345 40.0 38.0 41.0 34.0 41.0 26-27 38.22799997612316 40.0 38.0 41.0 34.0 41.0 28-29 38.180352740564174 40.0 37.5 41.0 34.0 41.0 30-31 38.14298050654723 40.0 37.5 41.0 34.0 41.0 32-33 37.92497597392649 40.0 37.0 41.0 33.5 41.0 34-35 37.698296188260954 40.0 36.0 41.0 33.0 41.0 36-37 37.47366682650886 40.0 36.0 41.0 33.0 41.0 38-39 37.285609824525096 40.0 36.0 41.0 33.0 41.0 40-41 37.07577714150412 40.0 36.0 41.0 32.0 41.0 42-43 36.7746652765038 39.0 35.0 41.0 31.0 41.0 44-45 36.319344819421424 39.0 35.0 41.0 30.5 41.0 46-47 35.771011124619214 39.0 35.0 41.0 29.0 41.0 48-49 35.253356188778284 39.0 35.0 40.0 26.5 41.0 50-51 34.91174422329624 39.0 35.0 40.0 24.0 41.0 52-53 34.663783206222305 38.5 35.0 40.0 23.5 41.0 54-55 34.43492764321629 38.0 34.0 40.0 23.0 41.0 56-57 34.132570202893476 38.0 34.0 40.0 22.5 41.0 58-59 33.849865394296216 38.0 34.0 40.0 20.5 41.0 60-61 33.433890990272175 37.5 33.0 40.0 18.5 41.0 62-63 33.06950549067908 37.0 33.0 40.0 17.0 41.0 64-65 32.678876355757005 36.5 32.0 39.0 12.5 41.0 66-67 32.241254608728184 36.0 32.0 39.0 8.0 40.0 68-69 31.746315504627134 35.5 31.0 39.0 7.0 40.0 70-71 31.260501334118615 35.0 31.0 38.0 4.0 40.0 72-73 30.767884253022906 35.0 30.5 37.0 2.0 39.5 74-75 30.295828118564444 34.5 30.0 37.0 2.0 39.0 76-77 29.085102043658807 33.5 28.5 35.5 2.0 38.0 78-79 29.684832633277555 34.0 29.5 36.0 2.0 38.0 80-81 29.482277413103212 34.0 29.0 35.5 2.0 37.0 82-83 29.062939358787375 34.0 29.0 35.0 2.0 37.0 84-85 28.636930711390647 34.0 29.0 35.0 2.0 36.5 86-87 28.311349061540575 34.0 29.0 35.0 2.0 36.0 88-89 27.870673068313636 34.0 27.5 35.0 2.0 36.0 90-91 27.316310470592683 33.0 26.0 35.0 2.0 35.0 92-93 26.830222711255345 33.0 25.5 35.0 2.0 35.0 94-95 26.277433000582995 32.5 25.0 35.0 2.0 35.0 96-97 25.697529144671783 32.0 23.5 34.0 2.0 35.0 98-99 24.85457211702041 32.0 18.0 34.0 2.0 35.0 100-101 22.434069071728025 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3235380700756636 1101 2 0.28338694429387346 1101 3 0.2724196376621961 1101 4 0.23347103704703898 1101 5 0.22345224509212613 1101 6 0.1282678504849173 1101 7 0.24764931877257368 1101 8 0.18250354964870752 1101 9 0.28169502236392674 1101 10-11 0.31138075105713625 1101 12-13 0.3037345926029431 1101 14-15 0.4788679203092414 1101 16-17 0.47483779783532043 1101 18-19 0.3538817077574379 1101 20-21 0.3196355952686716 1101 22-23 0.2774268844933587 1101 24-25 0.4147029648751044 1101 26-27 0.43828245717942593 1101 28-29 0.4312753687000779 1101 30-31 0.40655203775096993 1101 32-33 0.5434718471632962 1101 34-35 0.626412372578443 1101 36-37 0.5812228677500215 1101 38-39 0.8263767205663655 1101 40-41 0.6393976510887427 1101 42-43 0.6562021130368478 1101 44-45 0.6721629616968556 1101 46-47 0.7439347967458403 1101 48-49 0.6850329863573918 1101 50-51 0.39524530421982007 1101 52-53 0.33676996941450454 1101 54-55 0.5271861180299169 1101 56-57 0.59090491604249 1101 58-59 0.5790581279636626 1101 60-61 0.7600124838772118 1101 62-63 0.7893112221539695 1101 64-65 0.9098671765742665 1101 66-67 0.8446443355563353 1101 68-69 0.9335899563172134 1101 70-71 0.5905102013282963 1101 72-73 0.7512272508816267 1101 74-75 0.8543731823243661 1101 76-77 0.830281725380015 1101 78-79 0.8974046540353058 1101 80-81 0.9442894669115098 1101 82-83 0.8929850131787767 1101 84-85 0.8783059130955593 1101 86-87 0.9217796748546441 1101 88-89 1.0515734005897563 1101 90-91 0.8730988755118396 1101 92-93 0.9333354042886732 1101 94-95 1.0706313971580421 1101 96-97 1.2810467569994195 1101 98-99 1.367059620724298 1101 100-101 1.7017978788545598 1102 1 0.2314545734930178 1102 2 0.18388322220950215 1102 3 0.17955472102818248 1102 4 0.1899879496760022 1102 5 0.1303914292808912 1102 6 0.18131952433455467 1102 7 0.1781725872415123 1102 8 0.21354268595099768 1102 9 0.11776441420581563 1102 10-11 0.13806657231002362 1102 12-13 0.1282198130983474 1102 14-15 0.15566583942464973 1102 16-17 0.15454590806125879 1102 18-19 0.09608383217518224 1102 20-21 0.18860734225400222 1102 22-23 0.1237657465368045 1102 24-25 0.37234956275939624 1102 26-27 0.20885840076046236 1102 28-29 0.2346851027125254 1102 30-31 0.30630716377944367 1102 32-33 0.4615511645634953 1102 34-35 0.3516964263854163 1102 36-37 0.41435159527603815 1102 38-39 0.5603195180066152 1102 40-41 0.6565997755064856 1102 42-43 0.7773646658207909 1102 44-45 0.7105559354463651 1102 46-47 0.8177855215727945 1102 48-49 0.9320183156856743 1102 50-51 1.0521159236965545 1102 52-53 1.1266604835410092 1102 54-55 1.160632637554528 1102 56-57 1.1324768981068374 1102 58-59 1.2894832291782308 1102 60-61 1.2834028456376885 1102 62-63 1.1385483036915574 1102 64-65 1.2469606857815236 1102 66-67 1.1300008720588757 1102 68-69 1.1384830824327565 1102 70-71 1.2219835808067678 1102 72-73 1.1729677062383814 1102 74-75 1.0474078782453127 1102 76-77 1.3184809766098269 1102 78-79 1.1644533240975612 1102 80-81 1.2355893667852627 1102 82-83 1.298162193101284 1102 84-85 1.39704258106822 1102 86-87 1.323375164820252 1102 88-89 1.310889494795493 1102 90-91 1.2112872434540272 1102 92-93 1.395135064730102 1102 94-95 1.7432370738808665 1102 96-97 1.9947183429120905 1102 98-99 1.8712725413633642 1102 100-101 1.7929584726467347 1103 1 0.18939339110720255 1103 2 0.2375947441179349 1103 3 0.24196266736037586 1103 4 0.10385850753147707 1103 5 0.18062574770802087 1103 6 0.19234683643576744 1103 7 0.18275238530411997 1103 8 0.09388999641267048 1103 9 0.13196561380685523 1103 10-11 0.19195912740559606 1103 12-13 0.28221074094467014 1103 14-15 0.32181878172629297 1103 16-17 0.31776679587533607 1103 18-19 0.20627497589504173 1103 20-21 0.23062207084085173 1103 22-23 0.22844391735015535 1103 24-25 0.18475682594748832 1103 26-27 0.15560185282424044 1103 28-29 0.2098733089127478 1103 30-31 0.3126276130150103 1103 32-33 0.3400819948568383 1103 34-35 0.44666711950341664 1103 36-37 0.44888384650911206 1103 38-39 0.4259566865109363 1103 40-41 0.7009713512466718 1103 42-43 0.7142886045564367 1103 44-45 0.6943510741929231 1103 46-47 0.687662965520822 1103 48-49 0.7717868664637777 1103 50-51 0.9854465244534723 1103 52-53 0.8498101877396138 1103 54-55 0.8844388432355572 1103 56-57 1.0846404154791855 1103 58-59 1.2162939026216861 1103 60-61 1.215142403780085 1103 62-63 0.8550100271275056 1103 64-65 1.057183254130095 1103 66-67 1.0010259980989638 1103 68-69 1.1607658767653817 1103 70-71 1.1629879392661415 1103 72-73 1.0898318015506447 1103 74-75 0.901967181984844 1103 76-77 0.9446638406231607 1103 78-79 1.1004324745395522 1103 80-81 1.271967473383068 1103 82-83 0.9831412205773447 1103 84-85 1.0790232890787692 1103 86-87 1.3041405594726037 1103 88-89 1.3404696344301463 1103 90-91 1.1533545741367597 1103 92-93 1.230934548273158 1103 94-95 1.1659010626226483 1103 96-97 1.4022650358114888 1103 98-99 1.5688630921141424 1103 100-101 2.0079944743409293 1104 1 0.20629261530160647 1104 2 0.06515951954470722 1104 3 0.11158904948607073 1104 4 0.038485244807240804 1104 5 0.06683369202048794 1104 6 0.028147147389383065 1104 7 0.10396234213365574 1104 8 0.1478076963029693 1104 9 0.2844611349698525 1104 10-11 0.1960275687703188 1104 12-13 0.25924732929416905 1104 14-15 0.12464834126128466 1104 16-17 0.22738702206646622 1104 18-19 0.09521560269545404 1104 20-21 0.007134023613780016 1104 22-23 -0.008589158959757981 1104 24-25 0.07845054452657507 1104 26-27 -0.007486019195461324 1104 28-29 0.22683764395343076 1104 30-31 0.17035745430238336 1104 32-33 0.26016533674008 1104 34-35 0.2856775924843902 1104 36-37 0.2642797993767445 1104 38-39 0.37633334289140663 1104 40-41 0.2409799932317256 1104 42-43 0.5711818701218689 1104 44-45 0.7322850478486913 1104 46-47 0.7978897091103647 1104 48-49 1.0675706432085477 1104 50-51 1.3651009634347488 1104 52-53 1.4158493568462944 1104 54-55 1.240245941501641 1104 56-57 1.3571746874610042 1104 58-59 1.168831055265187 1104 60-61 1.1794557408490718 1104 62-63 1.2662047519651196 1104 64-65 1.3219456587328366 1104 66-67 1.3186554502997119 1104 68-69 1.2830065580221444 1104 70-71 1.5513948544326261 1104 72-73 1.5489678398121463 1104 74-75 1.6585512696832367 1104 76-77 1.3869776038722321 1104 78-79 1.6089116824759735 1104 80-81 1.7507433105507069 1104 82-83 1.6012009962803404 1104 84-85 1.5172873549946218 1104 86-87 1.3229477287686926 1104 88-89 1.4584968547100026 1104 90-91 1.6962468373897757 1104 92-93 1.795054920470431 1104 94-95 1.5526673817683943 1104 96-97 1.6119209743152823 1104 98-99 1.709017148322559 1104 100-101 1.7913539177813078 1105 1 -0.10662387324417111 1105 2 -0.11337418930936849 1105 3 -0.09600141496938619 1105 4 -0.2189581127505349 1105 5 -0.04982710713458971 1105 6 -0.1298293155069814 1105 7 -0.25538711847438833 1105 8 -0.2911806608776075 1105 9 -0.11810254848627721 1105 10-11 -0.1704006254610846 1105 12-13 -0.27723706731608644 1105 14-15 -0.3273061687595842 1105 16-17 -0.17951442888532654 1105 18-19 -0.04945837321422175 1105 20-21 -0.23299598367869834 1105 22-23 -0.08795773089125447 1105 24-25 -0.07315128613704047 1105 26-27 -0.1558470974764461 1105 28-29 -0.2372468985063918 1105 30-31 -0.09901476386846042 1105 32-33 -0.21462531882860247 1105 34-35 0.10718160334766935 1105 36-37 0.2289961471022508 1105 38-39 0.31220668008052854 1105 40-41 0.16065271181748386 1105 42-43 0.30346931951458345 1105 44-45 0.2141670102798514 1105 46-47 0.312254634802521 1105 48-49 0.4360451321063792 1105 50-51 0.12145987911861056 1105 52-53 0.06703312730923727 1105 54-55 0.23872457956837678 1105 56-57 0.13948791199390342 1105 58-59 0.2688354558988806 1105 60-61 0.2853161276019023 1105 62-63 0.0367808577815012 1105 64-65 0.30511009155402036 1105 66-67 0.2267293557992467 1105 68-69 0.06112032068968176 1105 70-71 0.3288705252149349 1105 72-73 0.13078190675207324 1105 74-75 0.3078144778709273 1105 76-77 -0.08925268757545268 1105 78-79 0.186372265371336 1105 80-81 0.40582792844996973 1105 82-83 0.19004979050671267 1105 84-85 0.2993585446745044 1105 86-87 0.506799917769623 1105 88-89 0.2995491090917781 1105 90-91 0.5466818714632531 1105 92-93 0.570987226151022 1105 94-95 0.9810564983726131 1105 96-97 0.9176864331127774 1105 98-99 0.9505616450157959 1105 100-101 0.8515954497047638 1106 1 -0.4281977474496266 1106 2 -0.3377621768552501 1106 3 -0.33948836898265355 1106 4 -0.11581374644827491 1106 5 -0.1974810179463944 1106 6 -0.3668007366889299 1106 7 -0.4597709281213582 1106 8 -0.23567926327094568 1106 9 -0.5283374060895554 1106 10-11 -0.6247943824864137 1106 12-13 -0.6276981283034573 1106 14-15 -0.4655225043443423 1106 16-17 -0.566457500038922 1106 18-19 -0.43390216555274463 1106 20-21 -0.533643060641559 1106 22-23 -0.3966696448663285 1106 24-25 -0.35208572296621554 1106 26-27 -0.5618319376718048 1106 28-29 -1.074236722338327 1106 30-31 -0.8498155278271184 1106 32-33 -0.6986150133446145 1106 34-35 -1.063338843488566 1106 36-37 -0.7173899703364981 1106 38-39 -0.49278740122934295 1106 40-41 -0.6591356079708319 1106 42-43 -0.9985355745955715 1106 44-45 -0.8491928162378741 1106 46-47 -0.8358643159480081 1106 48-49 -0.9512212288441972 1106 50-51 -1.166586356296044 1106 52-53 -1.295546327375483 1106 54-55 -1.2979787597665435 1106 56-57 -1.2973188800760411 1106 58-59 -1.619853380158375 1106 60-61 -1.6899149002607032 1106 62-63 -1.7243602059911396 1106 64-65 -1.949480623563005 1106 66-67 -1.753457953335431 1106 68-69 -1.7498844639993116 1106 70-71 -1.5995257584445106 1106 72-73 -2.0772053407088578 1106 74-75 -2.2241688304281695 1106 76-77 -1.7341213324440972 1106 78-79 -2.029418700419626 1106 80-81 -1.8957268467890138 1106 82-83 -1.7588032767674093 1106 84-85 -1.5482600529440944 1106 86-87 -1.4118180929536486 1106 88-89 -1.730377578729648 1106 90-91 -2.258294801934472 1106 92-93 -2.039120305009142 1106 94-95 -1.738250865671592 1106 96-97 -2.221847930105799 1106 98-99 -3.088402236053348 1106 100-101 -2.3076148279265674 1107 1 -0.07832059757247833 1107 2 0.020749581656509974 1107 3 -0.010460639954921191 1107 4 0.12174329110876414 1107 5 0.14263832217294237 1107 6 -0.13071790060609345 1107 7 0.18213851378808954 1107 8 0.11700588941420165 1107 9 0.12192584417369545 1107 10-11 0.0037068460148148574 1107 12-13 -0.06462336522869805 1107 14-15 -0.16345160227344735 1107 16-17 -0.21826515184658035 1107 18-19 0.030506398855813188 1107 20-21 0.03350330650480515 1107 22-23 -0.12945307764976377 1107 24-25 -6.290715095644828E-4 1107 26-27 -0.0316108074506829 1107 28-29 0.08262533452373333 1107 30-31 0.07342069540910501 1107 32-33 0.1429434282138189 1107 34-35 0.08127330112357356 1107 36-37 0.1917360387895144 1107 38-39 0.3656190571771205 1107 40-41 0.22998338114815908 1107 42-43 0.22410394804394684 1107 44-45 0.14849058144552885 1107 46-47 -0.4647867448704375 1107 48-49 -0.273860751483717 1107 50-51 -0.40874149845118524 1107 52-53 -0.16767689950605558 1107 54-55 0.16348140169361614 1107 56-57 0.06677378401662537 1107 58-59 0.18558712528212595 1107 60-61 0.21212090177510134 1107 62-63 0.01688233525140248 1107 64-65 -0.02828020236258766 1107 66-67 -0.09635019622767516 1107 68-69 -0.35248186659671177 1107 70-71 -0.22865878757754032 1107 72-73 -0.42034328384680464 1107 74-75 -0.25116075742009514 1107 76-77 -0.2267152250379887 1107 78-79 -0.26792343892436676 1107 80-81 -0.2449370959992656 1107 82-83 0.032878014913883646 1107 84-85 0.43647143178739256 1107 86-87 0.4549070737715155 1107 88-89 -0.045610993963062896 1107 90-91 0.4605890169493456 1107 92-93 0.534975869087031 1107 94-95 0.5444799170591921 1107 96-97 0.2672710590131935 1107 98-99 0.6682492205981099 1107 100-101 1.1249931046813657 1108 1 0.19212732185503256 1108 2 0.11754258457195732 1108 3 0.09216326675061737 1108 4 0.020073532296152052 1108 5 0.1709977178792812 1108 6 0.22915487967484438 1108 7 0.11280376690231719 1108 8 0.1751559026662548 1108 9 0.1507623138264904 1108 10-11 -0.11693985422372322 1108 12-13 0.06567083037162291 1108 14-15 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0.4987601604104448 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 4.0 5 21.0 6 31.0 7 89.0 8 174.0 9 367.0 10 566.0 11 1101.0 12 1541.0 13 2296.0 14 3026.0 15 4284.0 16 6202.0 17 8863.0 18 7832.0 19 4318.0 20 3441.0 21 3316.0 22 3368.0 23 3524.0 24 3961.0 25 4633.0 26 5454.0 27 6427.0 28 7817.0 29 9584.0 30 11924.0 31 14702.0 32 19072.0 33 24773.0 34 33697.0 35 49290.0 36 72893.0 37 103976.0 38 74620.0 39 5389.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.303361572024585 20.362113443103283 16.196176629828976 19.138348355043156 2 15.54959518802019 22.840787219521708 39.71097081254887 21.898646779909228 3 20.433603473284798 27.00610252318541 30.71974754217745 21.840546461352346 4 12.368403773337128 18.243500026861447 38.67471581582199 30.713380383979434 5 12.074643467602165 39.859794766281496 33.59652491597834 14.469036850137995 6 28.809401109079367 38.92721343311201 16.93126851698937 15.332116940819255 7 26.555825054369564 35.65966743536837 20.435394236527987 17.34911327373408 8 26.25000248717117 33.999231961542364 19.34024302646947 20.410522524816994 9 26.587660845359633 18.081734413893138 20.970832446242284 34.35977229450494 10-11 24.893399843606677 28.142441287837332 26.24751531600007 20.716643552555915 12-13 27.0931603480268 26.121510901954544 27.258617344629116 19.52671140538954 14-15 22.785472532676454 26.98501131165449 28.432047499000156 21.797468656668904 16-17 19.84712851113954 30.379005091736822 28.48139297503477 21.292473422088865 18-19 19.72456071582776 28.442493617918775 32.53309429960265 19.299851366650817 20-21 21.55462126352275 26.03461346375396 32.6709830693284 19.73978220339489 22-23 20.665417756478433 26.638697180401195 32.84746548501059 19.848419578109784 24-25 21.067931608762006 26.79310118409245 32.974020004815166 19.164947202330378 26-27 20.526111054182017 26.620829349678505 33.805336323271476 19.047723272868 28-29 19.492928376431635 28.184220370414586 33.13346147417683 19.189389778976942 30-31 20.12012041887942 26.84582921291976 33.00973578283215 20.024314585368668 32-33 20.026503295999238 27.290833878852876 32.83414149815253 19.848521326995357 34-35 20.943175102819655 26.88363421472047 32.88647157959247 19.286719102867412 36-37 20.750551656761054 26.50996762685121 33.35966429024806 19.379816426139676 38-39 20.153846459959528 27.48612655920761 33.13369639399975 19.226330586833114 40-41 20.155697380006067 27.700304928095665 32.538464216961565 19.605533474936703 42-43 20.066354134843785 27.71548923237978 31.803910993112822 20.414245639663612 44-45 20.779718213455 27.24914889002525 31.63940395440319 20.331728942116563 46-47 21.366193175600255 28.282717741887346 30.857238364515826 19.493850717996576 48-49 21.180849179929922 28.459406381882253 30.462971990473143 19.89677244771469 50-51 20.313622335990956 28.83646153142093 30.94130475009899 19.908611382489124 52-53 21.001673368763914 29.48053937788885 29.409406282395405 20.10838097095183 54-55 20.369832404457807 28.84700713718639 29.78009427373607 21.00306618461973 56-57 21.44986161379604 28.5601865577352 29.68468638761269 20.305265440856065 58-59 22.01275819323927 28.161443275584535 29.439650283835974 20.38614824734022 60-61 20.749076264627053 28.619182257913682 30.87156447046136 19.760177006997907 62-63 20.572586598325834 28.51462158188066 30.613595076594923 20.299196743198582 64-65 20.353815022115924 29.45885124527686 29.912212806344872 20.27512092626234 66-67 20.8631876779571 28.431351091072248 29.60579331806542 21.099667912905236 68-69 20.674063182106693 28.79119501610692 29.543315578247398 20.991426223538987 70-71 21.5133342220825 28.741750053225463 29.36901462257675 20.37590110211529 72-73 21.05599318714073 28.225711778645746 30.13386950111326 20.58442553310027 74-75 20.38664568157444 29.88017804166111 28.967386221867606 20.765790054896843 76-77 20.582037848776014 28.606248967823966 29.403636045278454 21.40807713812157 78-79 20.748379856699145 28.890283915563526 29.30693483014611 21.054401397591224 80-81 21.045149120834736 28.902819258265865 29.172727073753578 20.87930454714582 82-83 20.875466096804875 28.587900783559967 29.691908519672566 20.844724599962593 84-85 20.336703284458764 29.180487047807407 29.438157981133312 21.044651686600517 86-87 20.262685070406842 29.16655888924925 28.994844591596546 21.575911448747362 88-89 20.341478653107274 28.866208098627283 28.64664062764262 22.14567262062283 90-91 20.762639348242253 28.42296196355155 28.932495950393815 21.88190273781238 92-93 21.221931491448867 28.783440947937084 28.991274760478742 21.003352800135307 94-95 20.50294580553505 28.93823657574232 28.826114899349157 21.732702719373474 96-97 20.053862178881328 29.013150171415838 29.264354459696882 21.66863319000595 98-99 20.697727401609114 29.538605719152084 27.834878212085968 21.92878866715283 100-101 20.123581474719032 28.789906517878023 28.421152133571475 22.665359873831473 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 722.0 1 473.0 2 334.5 3 446.5 4 384.5 5 350.5 6 569.5 7 1144.5 8 1783.5 9 2034.0 10 1814.5 11 1554.5 12 1563.0 13 1630.5 14 1662.0 15 1729.0 16 1741.5 17 1736.0 18 1776.0 19 1839.5 20 1958.5 21 2136.5 22 2410.5 23 2752.5 24 3184.5 25 3725.0 26 4444.0 27 5270.0 28 5933.5 29 6752.0 30 7783.0 31 8559.0 32 9400.0 33 10377.0 34 11436.5 35 12255.5 36 13623.0 37 14883.0 38 15121.5 39 15267.0 40 15133.5 41 15462.0 42 15857.0 43 16224.5 44 17022.0 45 17512.0 46 17465.0 47 20341.0 48 26709.5 49 24730.0 50 17726.0 51 14910.5 52 13435.0 53 12564.0 54 11710.0 55 10675.0 56 9355.5 57 8160.0 58 7244.5 59 6403.5 60 5331.5 61 4116.5 62 3179.5 63 2412.5 64 1680.5 65 1216.0 66 923.0 67 712.0 68 520.0 69 366.0 70 257.5 71 197.5 72 168.0 73 150.5 74 126.5 75 110.0 76 85.0 77 58.5 78 44.0 79 32.0 80 17.0 81 13.0 82 11.0 83 5.5 84 1.5 85 0.5 86 0.5 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.038799870269151715 2 0.0 3 0.0 4 0.0 5 0.005969210810638725 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006267671351170662 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014923027026596813 24-25 0.0 26-27 8.953816215958088E-4 28-29 0.0013928158558157025 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.97947387375915E-4 38-39 0.0 40-41 2.984605405319363E-4 42-43 0.024573251170462752 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.989736936879575E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.012137395314965408 92-93 0.002785631711631405 94-95 0.0 96-97 0.0 98-99 8.953816215958088E-4 100-101 0.01522148756712875 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 502579.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.376396870707225 #Duplication Level Percentage of deduplicated Percentage of total 1 81.64973764238204 49.297169642855806 2 10.896134650662606 13.157387000501409 3 3.179138756396643 5.758348297897512 4 1.38494887789471 3.3447289238964664 5 0.7308417204845025 2.2062794882821404 6 0.4735248398933775 1.7153834196924396 7 0.3168623570593493 1.3391705196242107 8 0.2115841444760372 1.0219750622747423 9 0.16237538553708264 0.8823276647298897 >10 0.9189108906904603 9.833598381802402 >50 0.0452342638062861 1.910959903533734 >100 0.02443310573168785 2.8658417843538224 >500 0.002311239731375877 1.0330267199861924 >1k 0.003301771044822681 3.4403657146896394 >5k 6.603542089645363E-4 2.1934374758795983 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5575 1.1092783423103632 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5428 1.0800292093382335 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4420 0.8794637261007722 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3206 0.6379096619635918 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1577 0.313781514945909 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1268 0.2522986435963301 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1266 0.2519006962089542 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1137 0.22623308972320771 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1120 0.22285053693051243 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1114 0.22165669476838468 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1106 0.220064905218881 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1044 0.20772853621022763 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 996 0.19817779891320567 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 862 0.17151532395901936 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 843 0.1677348237789482 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 814 0.16196458666199742 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 647 0.12873597981610851 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 519 0.10326734702404995 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 9.948684684397876E-5 0.0 22-23 0.0 0.0 0.0 1.9897369368795752E-4 0.0 24-25 0.0 0.0 0.0 1.9897369368795752E-4 9.948684684397876E-5 26-27 0.0 0.0 0.0 8.953816215958088E-4 1.9897369368795752E-4 28-29 0.0 0.0 0.0 0.0013928158558157025 1.9897369368795752E-4 30-31 0.0 0.0 0.0 0.005173316035886895 1.9897369368795752E-4 32-33 0.0 0.0 0.0 0.009053303062802068 1.9897369368795752E-4 34-35 0.0 0.0 0.0 0.013729184864469068 1.9897369368795752E-4 36-37 0.0 0.0 0.0 0.018405066666136068 1.9897369368795752E-4 38-39 0.0 0.0 0.0 0.024274790629930817 1.9897369368795752E-4 40-41 0.0 0.0 0.0 0.03422347531432869 1.9897369368795752E-4 42-43 0.0 0.0 0.0 0.052429568286776806 1.9897369368795752E-4 44-45 0.0 0.0 0.0 0.08078331963731075 1.9897369368795752E-4 46-47 0.0 0.0 0.0 0.11251962378053998 1.9897369368795752E-4 48-49 0.0 0.0 0.0 0.16863020540054402 1.9897369368795752E-4 50-51 0.0 0.0 0.0 0.22304951062420036 1.9897369368795752E-4 52-53 0.0 0.0 0.0 0.2828411055774316 1.9897369368795752E-4 54-55 0.0 0.0 0.0 0.36362442521474236 1.9897369368795752E-4 56-57 0.0 0.0 0.0 0.45853487710389806 1.9897369368795752E-4 58-59 0.0 0.0 0.0 0.5787149880914244 1.9897369368795752E-4 60-61 0.0 0.0 0.0 0.7173996525919308 1.9897369368795752E-4 62-63 0.0 0.0 0.0 0.894187779433681 1.9897369368795752E-4 64-65 0.0 0.0 0.0 1.0528693001498273 1.9897369368795752E-4 66-67 0.0 0.0 0.0 1.236820479964344 1.9897369368795752E-4 68-69 0.0 0.0 0.0 1.4745940439214533 1.9897369368795752E-4 70-71 0.0 0.0 0.0 1.7170434896802296 1.9897369368795752E-4 72-73 0.0 0.0 0.0 1.9846631076905323 1.9897369368795752E-4 74-75 0.0 0.0 0.0 2.2946641224563704 1.9897369368795752E-4 76-77 0.0 0.0 0.0 2.608047690014903 1.9897369368795752E-4 78-79 0.0 0.0 0.0 2.936553258293721 1.9897369368795752E-4 80-81 0.0 0.0 0.0 3.3324114218859124 1.9897369368795752E-4 82-83 0.0 0.0 0.0 3.7714468770083904 1.9897369368795752E-4 84-85 0.0 0.0 0.0 4.22500741177009 1.9897369368795752E-4 86-87 0.0 0.0 0.0 4.707419132116543 1.9897369368795752E-4 88-89 0.0 0.0 0.0 5.323541174621303 1.9897369368795752E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGATCG 65 0.0058391304 29.232668 9 CGGTGTT 70 0.0083885705 27.14192 4 TAAGGTG 145 7.875133E-7 26.20599 5 CTGGTCG 95 0.0012251303 25.001621 9 GTACATG 3605 0.0 24.38473 1 AGTACTT 3780 0.0 24.317055 12-13 TACATGG 3565 0.0 24.115576 2 CTATTAC 100 0.0016488626 23.758633 1 GAGTACT 3665 0.0 23.200687 12-13 GTAATAC 105 0.0021949555 22.618265 3 ACATGGG 3655 0.0 22.482122 3 GTACTTT 3875 0.0 21.87989 14-15 GTTGTGC 220 5.619586E-8 21.592312 8 TACTTTT 4290 0.0 21.147287 14-15 ACTGATC 135 3.790182E-4 21.11248 8 AGTACAC 225 7.1340764E-8 21.110382 5 AGAGTAC 6310 0.0 21.079025 10-11 TATGTAC 320 1.8189894E-11 20.780533 3 CATGGGA 2625 0.0 20.62786 4 GCGTAGA 140 4.8346454E-4 20.364544 1 >>END_MODULE