FastQCFastQC Report
Thu 26 May 2016
SRR937560_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937560_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366831
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42591.161025104203298No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31880.8690650463019756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21460.5850105361869634No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20380.5555691858103595No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19190.5231291793768793No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15920.4339873129588285No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12290.3350316630819096No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT9680.2638817330051168No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC7890.21508542080685655No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7800.2126319749421396No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT6540.17828373283610163No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC5510.15020540793989604No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5490.1496601977477367No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5320.14502591111438237No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5040.13739296842415172No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4970.13548473275159406No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA4820.13139565631039907No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT4640.12648876458096506No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.12376271362016841No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG4400.11994624227505309No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC4350.11858321679465476No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA4150.11313111487306143No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAT156.6111865E-495.011217
ATAGCGA250.005016203557.0067256
AATAGCG459.5850317E-442.2503055
GTCCTAC608.2472776E-539.6096651
GTGTAGG851.6217742E-533.5517161
AAGACCG600.0039346331.687735
ATAGTAC600.00394254231.6747343
GGTATAC650.005831669629.2382143
GTATAGG856.373927E-427.9597631
CGTAAAC350.00839037627.14235140-41
GTACATG26850.026.9080181
GAGTACT27100.026.29102112-13
TACATGG26950.026.2682862
GTCTAAA1109.416644E-525.9263251
ACATGGG27000.025.86773
GTATCAA52300.025.5380081
CATGGGC4150.025.1974744
CTAGACA950.001220215525.0166284
GTACTTT29050.024.28261614-15
GGTATCA38550.024.166521
Produced by FastQC (version 0.11.5)