##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937560_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 366831 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.906049379687104 33.0 31.0 34.0 30.0 34.0 2 31.990295258579565 33.0 31.0 34.0 30.0 34.0 3 32.01588469894856 34.0 31.0 34.0 30.0 34.0 4 35.61747780313005 37.0 35.0 37.0 35.0 37.0 5 35.62857010448953 37.0 35.0 37.0 35.0 37.0 6 35.71893596778898 37.0 35.0 37.0 35.0 37.0 7 35.653764267469214 37.0 35.0 37.0 35.0 37.0 8 35.66418050819042 37.0 35.0 37.0 35.0 37.0 9 37.22225220878279 39.0 37.0 39.0 34.0 39.0 10-11 37.17616559123957 39.0 37.0 39.0 34.0 39.0 12-13 37.09245129228446 39.0 37.0 39.0 34.0 39.0 14-15 38.191458737129636 40.0 38.0 41.0 33.5 41.0 16-17 38.15606914355657 40.0 38.0 41.0 33.5 41.0 18-19 38.172890241010165 40.0 38.0 41.0 33.5 41.0 20-21 38.22807777968601 40.0 38.0 41.0 34.0 41.0 22-23 38.211683309207785 40.0 38.0 41.0 34.0 41.0 24-25 38.127002625187075 40.0 38.0 41.0 34.0 41.0 26-27 38.00718587033266 40.0 38.0 41.0 33.5 41.0 28-29 37.85411129375653 40.0 38.0 41.0 33.0 41.0 30-31 37.56816217822376 40.0 37.0 41.0 33.0 41.0 32-33 37.346245273709144 40.0 36.0 41.0 33.0 41.0 34-35 37.27889273262075 40.0 36.0 41.0 33.0 41.0 36-37 37.07969337378793 40.0 36.0 41.0 32.0 41.0 38-39 36.89044001188559 40.0 36.0 41.0 31.5 41.0 40-41 36.665698918575586 39.5 35.5 41.0 31.0 41.0 42-43 36.333322429129495 39.0 35.0 41.0 30.0 41.0 44-45 35.934488906335616 39.0 35.0 41.0 29.5 41.0 46-47 35.37644037717641 39.0 35.0 41.0 26.0 41.0 48-49 34.87635586959662 39.0 35.0 40.0 23.5 41.0 50-51 33.925563815489966 38.0 34.0 39.5 22.0 40.5 52-53 34.01836676834837 38.0 34.0 39.5 22.0 40.5 54-55 34.279213043608635 38.0 34.5 40.0 20.5 41.0 56-57 34.03181437773798 38.0 34.0 40.0 18.5 41.0 58-59 33.75441824709471 38.0 34.0 40.0 15.0 41.0 60-61 33.395194244761214 38.0 33.5 40.0 11.0 41.0 62-63 33.0753562267093 37.5 33.0 40.0 8.0 41.0 64-65 32.656623894927094 37.0 32.5 40.0 4.5 41.0 66-67 32.109669030152844 36.0 32.0 39.0 2.0 41.0 68-69 31.678113354651053 36.0 31.0 39.0 2.0 40.0 70-71 31.212483405164775 35.0 31.0 38.5 2.0 40.0 72-73 30.74503654271313 35.0 30.5 38.0 2.0 40.0 74-75 30.221473921233482 35.0 30.0 37.0 2.0 39.0 76-77 29.712671502681072 34.5 29.0 36.5 2.0 39.0 78-79 29.230647082716565 34.0 29.0 36.0 2.0 39.0 80-81 28.7023711191257 34.0 28.5 36.0 2.0 37.5 82-83 28.179695827233793 34.0 27.5 35.0 2.0 37.0 84-85 27.645691067548817 34.0 26.0 35.0 2.0 36.5 86-87 27.135045838546908 33.0 26.0 35.0 2.0 36.0 88-89 26.623435587504872 33.0 25.0 35.0 2.0 36.0 90-91 26.05321387778023 32.5 23.5 35.0 2.0 35.0 92-93 25.423256213351653 32.0 20.0 35.0 2.0 35.0 94-95 24.688157489416106 32.0 15.5 34.0 2.0 35.0 96-97 23.87431950952891 31.0 2.0 34.0 2.0 35.0 98-99 22.774164397229242 31.0 2.0 34.0 2.0 35.0 100-101 19.72752439134097 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2509890265969439 1101 2 0.30329667077624123 1101 3 0.2849802204637122 1101 4 0.16507791677786088 1101 5 0.12103777035028429 1101 6 0.17442634077881536 1101 7 0.22536358622595287 1101 8 0.24375048913559283 1101 9 0.4814292831064648 1101 10-11 0.5446728648101882 1101 12-13 0.3660150938022113 1101 14-15 0.6379373528553529 1101 16-17 0.49160464236868506 1101 18-19 0.4654892749194701 1101 20-21 0.5342613175135895 1101 22-23 0.4453951985576836 1101 24-25 0.5596121698803316 1101 26-27 0.6625101918681651 1101 28-29 0.5736558517586019 1101 30-31 0.5938822427602162 1101 32-33 0.8436279966191265 1101 34-35 0.8697127976723493 1101 36-37 0.8761566907923921 1101 38-39 1.1313484262819813 1101 40-41 1.2060519947340254 1101 42-43 1.139262206907361 1101 44-45 1.398268607612117 1101 46-47 1.5233833618689587 1101 48-49 1.784581648230109 1101 50-51 1.5966884805001698 1101 52-53 1.8682383766473762 1101 54-55 1.8430405026848007 1101 56-57 2.1575011527252173 1101 58-59 2.0736075983987234 1101 60-61 1.9792969207278688 1101 62-63 2.179758394130552 1101 64-65 2.1233353313798276 1101 66-67 2.075923994071072 1101 68-69 2.314967317670206 1101 70-71 2.46182063462825 1101 72-73 2.497891800626139 1101 74-75 2.441493527752087 1101 76-77 2.2770994036914587 1101 78-79 2.4524690057054137 1101 80-81 2.531901294254059 1101 82-83 2.4402561390005424 1101 84-85 2.6463483758146147 1101 86-87 2.733864086212055 1101 88-89 2.8907108848843563 1101 90-91 2.9590270620979275 1101 92-93 2.892083672274058 1101 94-95 3.3044587725301753 1101 96-97 3.616181709864673 1101 98-99 4.0555213103932175 1101 100-101 4.269833261009541 1102 1 0.21776153105327367 1102 2 0.23010148361046134 1102 3 0.09594835771861554 1102 4 0.30216570643917606 1102 5 0.12716522133067798 1102 6 0.1426192997805984 1102 7 0.2738261530708712 1102 8 0.1454606050001246 1102 9 0.38052130627936975 1102 10-11 0.3461295270205298 1102 12-13 0.36327166688598567 1102 14-15 0.3838709535683691 1102 16-17 0.3575248740977415 1102 18-19 0.09177046921536913 1102 20-21 0.13440336296813626 1102 22-23 0.12115555952024692 1102 24-25 0.05828919750956629 1102 26-27 0.2041350804599631 1102 28-29 0.24429143553757626 1102 30-31 0.25717881138766074 1102 32-33 0.25108956079024836 1102 34-35 0.48291745453509805 1102 36-37 0.41633327278882604 1102 38-39 0.539561995800689 1102 40-41 0.7366335455361721 1102 42-43 0.600756747905578 1102 44-45 0.8672692760613216 1102 46-47 0.8439186782682455 1102 48-49 0.9097069824547006 1102 50-51 0.9257465241721761 1102 52-53 0.4746889114067372 1102 54-55 0.631197811062691 1102 56-57 0.8494714067359155 1102 58-59 0.6904469477748378 1102 60-61 0.7466765998722522 1102 62-63 0.9288532025084493 1102 64-65 0.9878351085634307 1102 66-67 1.0617333746414808 1102 68-69 1.0753561322869594 1102 70-71 0.8083836916692526 1102 72-73 1.0641379013391514 1102 74-75 1.142459993883996 1102 76-77 1.4346305979873577 1102 78-79 1.5027558818194926 1102 80-81 1.6815642844501362 1102 82-83 1.4268593029934138 1102 84-85 1.4274173374901906 1102 86-87 1.4660527002940533 1102 88-89 1.4681117315866743 1102 90-91 1.7316011485506912 1102 92-93 1.7957295056073939 1102 94-95 1.4435797618349895 1102 96-97 1.513407645693551 1102 98-99 1.7361000759903682 1102 100-101 1.869599303790288 1103 1 0.07098983683839677 1103 2 -0.0533695907697016 1103 3 0.029083688297127708 1103 4 0.21781653288545755 1103 5 0.19338422959513935 1103 6 0.11384863862357264 1103 7 0.01876416959979821 1103 8 0.03133353888441803 1103 9 0.17181779388267415 1103 10-11 0.15451020883870825 1103 12-13 0.11767507597689786 1103 14-15 0.04606103621299695 1103 16-17 -0.08622512590225995 1103 18-19 -0.03348779524743151 1103 20-21 0.1713997472656601 1103 22-23 -0.19920859337231178 1103 24-25 -0.2397092529036584 1103 26-27 -0.3124584112755713 1103 28-29 -0.2661037090905296 1103 30-31 -0.14165899852969233 1103 32-33 0.15120577979851646 1103 34-35 0.344836359493776 1103 36-37 0.19023564882188282 1103 38-39 0.45561313216432353 1103 40-41 0.46576577694111876 1103 42-43 0.24612090079813953 1103 44-45 0.783978986805117 1103 46-47 1.0392053518219626 1103 48-49 0.6277209287356982 1103 50-51 0.733636503511029 1103 52-53 0.9752570932249185 1103 54-55 0.8166316953602148 1103 56-57 1.1559667579755768 1103 58-59 1.0723942618244209 1103 60-61 1.4590732940871263 1103 62-63 1.7710148760621607 1103 64-65 1.6019621746791302 1103 66-67 1.787185337451401 1103 68-69 1.871869562039027 1103 70-71 1.748750427206442 1103 72-73 1.95348449224824 1103 74-75 2.034208444297221 1103 76-77 2.15059134178901 1103 78-79 2.1602584644641247 1103 80-81 2.308953230731131 1103 82-83 2.161957443489282 1103 84-85 2.3803228126968 1103 86-87 2.6663497044262847 1103 88-89 2.735286318363535 1103 90-91 2.655400218798622 1103 92-93 2.8447868403181396 1103 94-95 2.7027481502647426 1103 96-97 2.80669276966049 1103 98-99 3.2396770388002842 1103 100-101 3.6764174856084715 1104 1 -0.02451860728664812 1104 2 0.03840187886738278 1104 3 0.06451308103877196 1104 4 0.07476946137988705 1104 5 0.044408304334631055 1104 6 0.1943732523497701 1104 7 0.23899413726059038 1104 8 -0.020732576818055293 1104 9 0.15022239323589304 1104 10-11 0.2169608017240776 1104 12-13 0.23842606411918865 1104 14-15 0.17883142787666628 1104 16-17 0.27069187014517126 1104 18-19 0.10548094352368054 1104 20-21 -0.08048670620183174 1104 22-23 0.08826894884830949 1104 24-25 -0.05550156336000356 1104 26-27 -0.0064936250736309375 1104 28-29 0.11963110945061572 1104 30-31 0.1354520327315356 1104 32-33 0.2039366457704901 1104 34-35 0.25450218773667643 1104 36-37 0.18198915223546663 1104 38-39 0.12438783572164169 1104 40-41 0.051356865694266673 1104 42-43 0.2566670739925385 1104 44-45 0.39141700333404117 1104 46-47 0.5332405656200265 1104 48-49 0.3440881583440287 1104 50-51 0.18670872772948854 1104 52-53 0.3406721129877539 1104 54-55 0.2554567043433309 1104 56-57 0.22073499369243166 1104 58-59 0.8725739240594166 1104 60-61 0.9008267975007058 1104 62-63 0.7826390226665509 1104 64-65 0.8704314524369465 1104 66-67 0.8716086216770513 1104 68-69 0.6964653220102832 1104 70-71 0.7033318141988971 1104 72-73 0.8469627531178112 1104 74-75 0.7926885017891365 1104 76-77 0.9401888983826154 1104 78-79 0.701526879843211 1104 80-81 0.9041742152801753 1104 82-83 0.8181018919262186 1104 84-85 0.9857486121937491 1104 86-87 1.2039170777644088 1104 88-89 1.3125410794127603 1104 90-91 1.4723064351119568 1104 92-93 1.3379417091647063 1104 94-95 1.6206425088705636 1104 96-97 1.770077606167856 1104 98-99 1.5638236431840404 1104 100-101 1.607247525134163 1105 1 -0.03881827563429496 1105 2 0.08511715759165739 1105 3 -0.04323986067218755 1105 4 0.12789062806927376 1105 5 -0.05458765223254147 1105 6 0.05703283165102846 1105 7 0.12250692632061089 1105 8 0.04065718284756059 1105 9 0.07773590920518103 1105 10-11 -0.21544799387811508 1105 12-13 -0.05270109957169211 1105 14-15 -0.040371470674053 1105 16-17 0.2039525125512398 1105 18-19 0.020551817177754117 1105 20-21 0.040367639352673734 1105 22-23 0.12042900518377309 1105 24-25 -0.10515287406828122 1105 26-27 -0.04237909143137131 1105 28-29 -0.13283924628061072 1105 30-31 -0.013115728842223007 1105 32-33 0.0788798559835655 1105 34-35 0.2861681729258976 1105 36-37 0.09406152513992794 1105 38-39 0.34986600572754867 1105 40-41 0.5754527722864253 1105 42-43 0.24516146316158682 1105 44-45 0.05037729591502682 1105 46-47 0.4463465126465067 1105 48-49 0.07274087743903124 1105 50-51 0.05664875771450539 1105 52-53 -0.17085053477926948 1105 54-55 0.47178036069696816 1105 56-57 0.449188948533191 1105 58-59 0.641939241400749 1105 60-61 0.5753077388482168 1105 62-63 0.5520327349013385 1105 64-65 0.7290021670796278 1105 66-67 1.286940728350995 1105 68-69 1.1274884468115154 1105 70-71 1.1048015338845083 1105 72-73 0.7437927472116712 1105 74-75 1.2540146568934034 1105 76-77 1.1874061988233038 1105 78-79 0.9762815479636942 1105 80-81 0.8664601177834719 1105 82-83 0.7571721295346876 1105 84-85 1.1603147382216434 1105 86-87 1.365012666333758 1105 88-89 0.9796223115239826 1105 90-91 1.3921533298464048 1105 92-93 1.3550865458372776 1105 94-95 1.2025106657012365 1105 96-97 1.2720936436036858 1105 98-99 1.520362419250553 1105 100-101 2.348961895221848 1106 1 -0.26812862046188 1106 2 -0.17959548608650522 1106 3 -0.18300997561471632 1106 4 -0.2725691420456684 1106 5 -0.1236681120026546 1106 6 -0.10802463961334041 1106 7 -0.02140111965640301 1106 8 -0.001641667727149354 1106 9 -0.1621491482660815 1106 10-11 -0.27745948813099375 1106 12-13 0.05540234870417038 1106 14-15 -0.5087048040073938 1106 16-17 -0.42501300469013614 1106 18-19 -0.16887347017856058 1106 20-21 0.033011317513590654 1106 22-23 -0.22980087987369302 1106 24-25 -0.25141724188437564 1106 26-27 -0.2466319649945845 1106 28-29 0.10049408705272356 1106 30-31 -5.295219456726841E-4 1106 32-33 0.2875005456387356 1106 34-35 0.23260495453509833 1106 36-37 -0.1685246817566295 1106 38-39 -0.39938686783566624 1106 40-41 -0.20063918173656248 1106 42-43 0.3617131872995216 1106 44-45 0.22687154878859417 1106 46-47 0.7218637540258186 1106 48-49 0.6149247854850088 1106 50-51 0.7897522059903608 1106 52-53 0.22162072958854395 1106 54-55 0.6155159928808729 1106 56-57 0.48987860370560554 1106 58-59 0.3022840689869568 1106 60-61 0.7207675089631564 1106 62-63 0.8230672176599612 1106 64-65 0.8763010176543418 1106 66-67 1.0411936019142054 1106 68-69 0.7865114353172693 1106 70-71 0.6950314189419764 1106 72-73 1.108332977096726 1106 74-75 1.3630376453991495 1106 76-77 1.1931533252600843 1106 78-79 0.951856260607375 1106 80-81 1.051460117783467 1106 82-83 1.1396433939025066 1106 84-85 1.0861768071871616 1106 86-87 1.314035654839504 1106 88-89 1.303357943707887 1106 90-91 1.4493947091567527 1106 92-93 1.5675003389407287 1106 94-95 1.6232577921380198 1106 96-97 1.4443924941783983 1106 98-99 0.9777762123540015 1106 100-101 1.5067205159115034 1107 1 -0.10649340033609533 1107 2 -0.06475271879090627 1107 3 0.027493169039370713 1107 4 -0.056657192360134445 1107 5 0.054759560953321795 1107 6 0.1424156119589881 1107 7 -0.20291055361866484 1107 8 0.0638300303860575 1107 9 0.3346433045641035 1107 10-11 0.15691158105139635 1107 12-13 0.07719480153436109 1107 14-15 0.02928261737626059 1107 16-17 -0.23545325626246694 1107 18-19 -0.21550869030435393 1107 20-21 -0.23142264475055896 1107 22-23 -0.06209647735796864 1107 24-25 0.2973248964804114 1107 26-27 -0.06068856876817108 1107 28-29 -0.13142415194099044 1107 30-31 -0.0658754338953571 1107 32-33 0.31092821859471087 1107 34-35 -0.1930554228233916 1107 36-37 0.37732437484714154 1107 38-39 0.3235376604662221 1107 40-41 0.46643628996154973 1107 42-43 0.35995218101020754 1107 44-45 0.6406451336942496 1107 46-47 0.6164235024534932 1107 48-49 0.40288076032777553 1107 50-51 0.3016389984431953 1107 52-53 0.5059603522300549 1107 54-55 0.6316480683525754 1107 56-57 0.18465847791944157 1107 58-59 0.13278721364104484 1107 60-61 0.808597697642405 1107 62-63 0.9152999220624807 1107 64-65 1.0926217723713165 1107 66-67 1.2099671868198634 1107 68-69 1.112957976197766 1107 70-71 0.8686163246023568 1107 72-73 0.7754398953357224 1107 74-75 0.9992326139526071 1107 76-77 1.267681627146878 1107 78-79 1.1458814178400765 1107 80-81 1.1835984825633439 1107 82-83 0.994360375034578 1107 84-85 1.0155793229104297 1107 86-87 0.8308281076696957 1107 88-89 1.3157478808148042 1107 90-91 1.4005896777102116 1107 92-93 1.5757707791923004 1107 94-95 1.5755533896222964 1107 96-97 1.7808390350589 1107 98-99 1.892304514240795 1107 100-101 3.3457142266033273 1108 1 -0.5420024943357546 1108 2 -0.6540099005009203 1108 3 -0.7440009666057072 1108 4 -0.8512177906943208 1108 5 -0.438713157047701 1108 6 -0.08478139637009718 1108 7 -0.37671643497171914 1108 8 -0.3729029289884096 1108 9 -0.19268968880662385 1108 10-11 -0.23525228092378114 1108 12-13 -0.26635440805258526 1108 14-15 -0.22393002923261918 1108 16-17 -0.5632112028883327 1108 18-19 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-0.9693825976797044 2316 60-61 -0.5317324910368413 2316 62-63 -0.697766115673371 2316 64-65 -0.7336989823456648 2316 66-67 -0.9963063980857925 2316 68-69 -0.6076552313494012 2316 70-71 -0.48496858105801977 2316 72-73 -0.5658336895699385 2316 74-75 -0.8427956879341849 2316 76-77 -0.8993466747399168 2316 78-79 -1.2389770727259624 2316 80-81 -1.4143732155498618 2316 82-83 -1.2228566060974977 2316 84-85 -0.6421565261461701 2316 86-87 -1.008464345160494 2316 88-89 -0.9433087229587791 2316 90-91 -0.7139386241765813 2316 92-93 -0.5533329943926049 2316 94-95 -0.8609088745286471 2316 96-97 -1.2897741724882685 2316 98-99 -1.7972237876460007 2316 100-101 -2.857446150755166 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1638.0 3 315.0 4 273.0 5 261.0 6 294.0 7 376.0 8 433.0 9 557.0 10 690.0 11 936.0 12 1435.0 13 1987.0 14 2363.0 15 3228.0 16 4387.0 17 6355.0 18 5384.0 19 3148.0 20 2830.0 21 2831.0 22 2985.0 23 3243.0 24 3539.0 25 3885.0 26 4507.0 27 5098.0 28 5879.0 29 7145.0 30 8769.0 31 10946.0 32 13844.0 33 18567.0 34 25045.0 35 36302.0 36 50845.0 37 69456.0 38 51866.0 39 5188.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.718162496614994 19.410913527160726 15.765776855050701 20.105147121173577 2 14.675706338249567 21.1090911576156 41.47842370726773 22.736778796867096 3 19.476013811845053 26.818819322366604 30.91890325420411 22.786263611584236 4 11.571655471994182 17.212458207922932 39.35603979234498 31.85984652773791 5 11.802624384909786 38.768726079825036 34.647621651175506 14.781027884089667 6 28.379714880526308 37.63298380971636 18.156832102988346 15.830469206768994 7 25.77028669771762 35.33662054553153 21.2820127572972 17.61107999945365 8 25.680870071221918 33.38469398069604 20.351656517474915 20.582779430607122 9 26.414599497322698 17.3136815648563 21.92574582012895 34.34597311769206 10-11 24.135151823134382 27.512034927244017 26.96356463343333 21.389248616188276 12-13 26.227192498319663 25.692626735665225 27.991409789124532 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19.78179573261509 26.886409764012686 33.3699829818154 19.96181152155682 40-41 19.756136534041325 27.14401410518524 33.24401506119121 19.855834299582227 42-43 19.560122268690638 27.08991774999385 32.28674684972369 21.063213131591816 44-45 19.722082225308984 26.996321837196565 32.269356792718526 21.01223914477593 46-47 20.603389163745835 27.565571822351654 31.7006739534871 20.130365060415407 48-49 20.412947114629365 27.454767742860852 31.324695548831716 20.80758959367807 50-51 19.625367788678254 27.986908498821162 31.862042022844562 20.525681689656018 52-53 20.258120748705913 28.566647039617038 30.44346658324032 20.731765628436726 54-55 19.78633803721122 27.78889509637463 30.97278890192613 21.451977964488023 56-57 20.535609423011266 27.7746671218846 30.462546944349604 21.22717651075452 58-59 20.971305442598595 27.50971551232088 30.60898442513935 20.909994619941173 60-61 20.459288192073423 27.47316380322854 31.70508863456338 20.36245937013466 62-63 20.05129171548666 27.81592337378223 31.468887668958136 20.66389724177298 64-65 19.767937329968206 28.257235569831852 31.139718334480538 20.835108765719404 66-67 20.169206817436905 27.603381405003823 30.562520485086857 21.66489129247241 68-69 19.91327920681202 27.52395064426038 31.021461689211797 21.5413084597158 70-71 20.618666520990956 27.47056895468575 30.55174674278223 21.359017781541066 72-73 20.1450777768217 27.10299265596681 31.3889469363895 21.362982630821996 74-75 19.807217922695944 28.25758548438648 30.551875233909104 21.383321359008473 76-77 19.77280984263046 27.535436146645143 30.553620646796748 22.138133363927658 78-79 19.95853439009633 27.450343020023954 30.812116808459205 21.77900578142051 80-81 20.3659289448812 27.540960768352445 30.408214553023637 21.684895733742714 82-83 20.13545090894689 27.27974204509294 30.959505395229037 21.62530165073113 84-85 19.951738255830946 27.545929912585336 30.594449762856485 21.907882068727236 86-87 19.92996869895827 27.62166709457503 30.6537165207226 21.794647685744096 88-89 19.781208814471356 27.379763863945207 30.10263519096428 22.73639213061916 90-91 19.920324166196632 27.252167048839347 30.34196146770698 22.485547317257044 92-93 20.50976707599528 27.576421360835557 30.248437704846392 21.665373858322774 94-95 20.008276235050538 27.420013465953396 30.113750431907484 22.45795986708858 96-97 19.380290692751256 27.67317415615243 30.572316719067118 22.374218432029195 98-99 20.196256512861837 28.139660341024715 29.08065364208111 22.583429504032342 100-101 20.371032938220353 26.907876768449228 29.885972578794995 22.835117714535425 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 686.0 1 428.0 2 283.5 3 345.5 4 247.0 5 216.5 6 375.0 7 859.0 8 1536.0 9 1779.0 10 1411.5 11 1079.0 12 1016.5 13 1077.5 14 1126.5 15 1099.0 16 1119.5 17 1182.0 18 1209.0 19 1226.0 20 1313.5 21 1512.0 22 1745.0 23 2003.5 24 2298.5 25 2658.0 26 3310.5 27 3979.0 28 4465.0 29 5030.0 30 5689.5 31 6266.0 32 6901.5 33 7676.0 34 8549.0 35 9252.0 36 10003.0 37 10833.0 38 11068.0 39 11061.0 40 11160.5 41 11420.0 42 11594.5 43 11813.0 44 12407.5 45 13034.0 46 13265.5 47 14980.5 48 18833.0 49 17938.5 50 13335.5 51 10806.5 52 9649.5 53 9159.0 54 8515.0 55 7707.5 56 6748.5 57 5790.5 58 5121.5 59 4492.5 60 3733.5 61 2962.0 62 2298.5 63 1684.5 64 1223.5 65 945.5 66 686.0 67 497.0 68 360.5 69 264.5 70 198.0 71 136.5 72 103.0 73 92.5 74 61.5 75 58.0 76 58.0 77 33.0 78 23.0 79 18.5 80 15.0 81 9.0 82 5.5 83 3.5 84 1.5 85 1.5 86 2.0 87 2.0 88 1.0 89 1.5 90 2.5 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3402111599074233 2 0.2824188795385341 3 0.28841619165228677 4 0.28132845915421545 5 0.28105585405813577 6 0.22053752272844987 7 0.20881550359702425 8 0.21590323609509557 9 0.21563063099901592 10-11 0.22162794311276854 12-13 0.22708004503436185 14-15 0.22067382527648974 16-17 0.20867920104898444 18-19 0.20349970422347077 20-21 0.20309079657935125 22-23 0.21331348768233874 24-25 0.20867920104898444 26-27 0.21467651316273706 28-29 0.2172662615754939 30-31 0.22162794311276854 32-33 0.21944710234413123 34-35 0.21931079979609136 36-37 0.20840659595290473 38-39 0.20499903225190894 40-41 0.1975023921097181 42-43 0.20499903225190894 44-45 0.2048627297038691 46-47 0.2131771851342989 48-49 0.2190381947000117 50-51 0.21590323609509557 52-53 0.2288519781588797 54-55 0.21413130297057775 56-57 0.19245919783224427 58-59 0.1810097837968983 60-61 0.1965482742734393 62-63 0.18809751629496962 64-65 0.1970934844655986 66-67 0.1938222233126426 68-69 0.19450373605284177 70-71 0.1965482742734393 72-73 0.2048627297038691 74-75 0.2099059239813429 76-77 0.20445382205974957 78-79 0.2029544940313114 80-81 0.20418121696366992 82-83 0.19641197172539943 84-85 0.19150507999596544 86-87 0.19327701312048326 88-89 0.19859281249403676 90-91 0.1969571819175588 92-93 0.19164138254400528 94-95 0.1969571819175588 96-97 0.20172777109895296 98-99 0.19859281249403676 100-101 0.18837012139104928 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 366831.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.61541284191955 #Duplication Level Percentage of deduplicated Percentage of total 1 84.94468867888813 55.73680816393575 2 9.14556793063775 12.001804308852316 3 2.5119245549667877 4.944629001057025 4 1.0500322210178918 2.755931907176268 5 0.5943206553934149 1.949829758205956 6 0.374540220341992 1.4745366710185992 7 0.2538965063674284 1.166166685909391 8 0.2030420876203763 1.0658152322796957 9 0.14319692250799265 0.845633266924887 >10 0.7256181601110471 8.213586101779526 >50 0.030324691693428617 1.3922918736458334 >100 0.016616269420915375 2.5387248455470885 >500 0.003323253884183075 1.4519848885794868 >1k 0.0029078471486601904 4.462257295088188 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4259 1.161025104203298 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3188 0.8690650463019756 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2146 0.5850105361869634 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2038 0.5555691858103595 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1919 0.5231291793768793 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1592 0.4339873129588285 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1229 0.3350316630819096 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 968 0.2638817330051168 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 789 0.21508542080685655 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 780 0.2126319749421396 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 654 0.17828373283610163 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 551 0.15020540793989604 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 549 0.1496601977477367 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 532 0.14502591111438237 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 504 0.13739296842415172 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 497 0.13548473275159406 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 482 0.13139565631039907 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 464 0.12648876458096506 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 454 0.12376271362016841 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 440 0.11994624227505309 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 435 0.11858321679465476 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 415 0.11313111487306143 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.3630254803983306E-4 0.0 24-25 0.0 0.0 0.0 5.452101921593322E-4 0.0 26-27 0.0 0.0 0.0 0.0010904203843186645 0.0 28-29 0.0 0.0 0.0 0.002044538220597496 0.0 30-31 0.0 0.0 0.0 0.00667882485395182 0.0 32-33 0.0 0.0 0.0 0.010631598747106978 0.0 34-35 0.0 0.0 0.0 0.014448070092222303 0.0 36-37 0.0 0.0 0.0 0.018673449081457127 0.0 38-39 0.0 0.0 0.0 0.023852945906970784 0.0 40-41 0.0 0.0 0.0 0.033121519173679434 0.0 42-43 0.0 0.0 0.0 0.05220387589925606 0.0 44-45 0.0 0.0 0.0 0.07755614983466501 0.0 46-47 0.0 0.0 0.0 0.10863313078774695 0.0 48-49 0.0 0.0 0.0 0.1686062519252735 0.0 50-51 0.0 0.0 0.0 0.22980609599515855 0.0 52-53 0.0 0.0 0.0 0.30163753881215055 0.0 54-55 0.0 0.0 0.0 0.3971856249880735 0.0 56-57 0.0 0.0 0.0 0.5103167398611349 0.0 58-59 0.0 0.0 0.0 0.6538433229470791 0.0 60-61 0.0 0.0 0.0 0.810727555740927 0.0 62-63 0.0 0.0 0.0 1.0161354956369555 0.0 64-65 0.0 0.0 0.0 1.198508304914252 0.0 66-67 0.0 0.0 0.0 1.4150930537495467 0.0 68-69 0.0 0.0 0.0 1.7145497517930601 0.0 70-71 0.0 0.0 0.0 2.0111440963277367 0.0 72-73 0.0 0.0 0.0 2.360351224405789 0.0 74-75 0.0 0.0 0.0 2.7492223939634326 0.0 76-77 0.0 0.0 0.0 3.1454539011152276 0.0 78-79 0.0 0.0 0.0 3.5724897841240244 0.0 80-81 0.0 0.0 0.0 4.081443498504761 0.0 82-83 0.0 0.0 0.0 4.679402776755508 0.0 84-85 0.0 0.0 0.0 5.275590121881739 0.0 86-87 0.0 0.0 0.0 5.8649623396059765 0.0 88-89 0.0 0.0 0.0 6.619124337910373 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCGAT 15 6.6111865E-4 95.01121 7 ATAGCGA 25 0.0050162035 57.006725 6 AATAGCG 45 9.5850317E-4 42.250305 5 GTCCTAC 60 8.2472776E-5 39.609665 1 GTGTAGG 85 1.6217742E-5 33.551716 1 AAGACCG 60 0.00393463 31.68773 5 ATAGTAC 60 0.003942542 31.674734 3 GGTATAC 65 0.0058316696 29.238214 3 GTATAGG 85 6.373927E-4 27.959763 1 CGTAAAC 35 0.008390376 27.142351 40-41 GTACATG 2685 0.0 26.908018 1 GAGTACT 2710 0.0 26.291021 12-13 TACATGG 2695 0.0 26.268286 2 GTCTAAA 110 9.416644E-5 25.926325 1 ACATGGG 2700 0.0 25.8677 3 GTATCAA 5230 0.0 25.538008 1 CATGGGC 415 0.0 25.197474 4 CTAGACA 95 0.0012202155 25.016628 4 GTACTTT 2905 0.0 24.282616 14-15 GGTATCA 3855 0.0 24.16652 1 >>END_MODULE