FastQCFastQC Report
Thu 26 May 2016
SRR937560_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937560_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366831
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42551.1599346838189795No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38581.0517104606753518No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32770.8933268998530659No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23730.6468918929970477No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12420.3385755293309453No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9980.2720598858875068No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8870.24180072022266386No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA8790.23961987945402652No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8610.2347129877245925No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG8150.2221731533049279No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT7950.21672105138333458No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7540.20554424244406824No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT7360.20063735071463423No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA7280.19845650994599692No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6020.164108267839959No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5250.1431176754418247No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC4760.1297600257339211No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA3950.1076790129514681No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT3750.10222691102987479No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGA200.002069987171.275672
CCGCTCA250.00501952756.997229
GGACCGA601.4899852E-647.497686
TAAGGTG1151.0298572E-733.0553865
GGTACCT600.0039405231.6780765
GTAAGGT902.4025934E-531.6780764
ACGGTGT753.0752725E-431.6651236
TATAGGT953.4758727E-530.010813
AGGACCG804.4782815E-429.6981965
AAGGGTA1354.198646E-728.158294
AACGGTG856.3853315E-427.9512445
CGCTATA350.008391621527.14153544-45
CGGTGTC700.00838624827.1415357
TAGTTTA1457.866365E-726.2056189
TAAGGGT1658.348252E-825.9184273
GCTAAGG1701.111639E-725.1629831
CTAAGGG1701.11458576E-725.156122
GACCTAT950.001225310824.9987816
CTATTAG1000.001645431223.765041
AGGGTAG1652.4148176E-623.03865
Produced by FastQC (version 0.11.5)