##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937560_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 366831 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12633065362524 33.0 31.0 34.0 31.0 34.0 2 32.344251167431324 34.0 31.0 34.0 31.0 34.0 3 32.420182590893354 34.0 31.0 34.0 31.0 34.0 4 36.063045380570344 37.0 35.0 37.0 35.0 37.0 5 35.92187955761645 37.0 35.0 37.0 35.0 37.0 6 36.04622019404031 37.0 35.0 37.0 35.0 37.0 7 36.00701140307117 37.0 35.0 37.0 35.0 37.0 8 36.00805002848723 37.0 35.0 37.0 35.0 37.0 9 37.640758278335255 39.0 37.0 39.0 35.0 39.0 10-11 37.58845081249949 39.0 37.0 39.0 35.0 39.0 12-13 37.51409232044184 39.0 37.0 39.0 35.0 39.0 14-15 38.59652537544537 40.0 38.0 41.0 34.0 41.0 16-17 38.60187797650689 40.0 38.0 41.0 34.5 41.0 18-19 38.67607154248142 40.0 38.0 41.0 34.0 41.0 20-21 38.6358663798861 40.0 38.0 41.0 34.0 41.0 22-23 38.48574275347504 40.0 38.0 41.0 34.0 41.0 24-25 38.40155957375467 40.0 38.0 41.0 34.0 41.0 26-27 38.269215251709916 40.0 38.0 41.0 34.0 41.0 28-29 38.14753933010024 40.0 38.0 41.0 34.0 41.0 30-31 38.15725361269904 40.0 37.0 41.0 34.0 41.0 32-33 37.97906665467204 40.0 37.0 41.0 33.5 41.0 34-35 37.77514304952417 40.0 36.0 41.0 33.0 41.0 36-37 37.559250717632914 40.0 36.0 41.0 33.0 41.0 38-39 37.37881885663971 40.0 36.0 41.0 33.0 41.0 40-41 37.17284253511835 40.0 36.0 41.0 33.0 41.0 42-43 36.88816375933332 40.0 35.5 41.0 31.5 41.0 44-45 36.427532024283664 39.0 35.0 41.0 30.5 41.0 46-47 35.86921225305386 39.0 35.0 41.0 29.5 41.0 48-49 35.35425986353388 39.0 35.0 40.5 26.5 41.0 50-51 35.00390370497586 39.0 35.0 40.0 24.5 41.0 52-53 34.80265980792245 39.0 35.0 40.0 24.0 41.0 54-55 34.59084292221759 38.5 34.5 40.0 23.5 41.0 56-57 34.28727806537616 38.0 34.0 40.0 23.0 41.0 58-59 34.01357164470832 38.0 34.0 40.0 22.0 41.0 60-61 33.610429598370914 38.0 33.5 40.0 20.0 41.0 62-63 33.274601928408444 37.0 33.0 40.0 18.5 41.0 64-65 32.86684740384537 36.5 33.0 39.5 15.5 41.0 66-67 32.4158822455027 36.0 32.0 39.0 10.5 40.0 68-69 31.911179807595325 35.5 31.5 39.0 7.5 40.0 70-71 31.464092456744385 35.0 31.0 38.0 6.0 40.0 72-73 30.994402054352 35.0 31.0 37.5 2.0 39.5 74-75 30.506205855012254 35.0 30.0 37.0 2.0 39.0 76-77 29.317940686583196 33.5 28.5 35.5 2.0 38.0 78-79 29.860438458036533 34.0 30.0 36.0 2.0 38.0 80-81 29.620469644059526 34.0 30.0 36.0 2.0 37.5 82-83 29.24264579602051 34.0 29.0 35.0 2.0 37.0 84-85 28.849283457504953 34.0 29.0 35.0 2.0 36.5 86-87 28.44825955276408 34.0 29.0 35.0 2.0 36.0 88-89 27.959842543296503 34.0 28.0 35.0 2.0 36.0 90-91 27.412387448170957 33.0 26.5 35.0 2.0 35.0 92-93 26.95410147997307 33.0 25.5 35.0 2.0 35.0 94-95 26.443208180333723 33.0 25.0 35.0 2.0 35.0 96-97 25.856079502550223 32.0 24.0 34.0 2.0 35.0 98-99 25.05135471102497 32.0 19.0 34.0 2.0 35.0 100-101 22.731392657654343 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07011237109186652 1101 2 0.028055060707266932 1101 3 0.018272418015961023 1101 4 0.08424588228789531 1101 5 -0.08392685550222012 1101 6 -0.016222399518376562 1101 7 0.05921233899302791 1101 8 0.019702220564937534 1101 9 0.12948734928854577 1101 10-11 0.13646631516562024 1101 12-13 0.19369172792467282 1101 14-15 0.09371477688993224 1101 16-17 -0.02091571472490017 1101 18-19 0.05700542436184719 1101 20-21 0.07301702645628438 1101 22-23 0.10597908720173876 1101 24-25 0.13314772862378987 1101 26-27 -0.0211593339056364 1101 28-29 0.20879928722767715 1101 30-31 0.39580428286483027 1101 32-33 0.2523986598735277 1101 34-35 0.13345557641051187 1101 36-37 0.37652115914086437 1101 38-39 0.5533832589184797 1101 40-41 0.34059094616257113 1101 42-43 0.25085461769151607 1101 44-45 0.42494935877257944 1101 46-47 0.2913238831078573 1101 48-49 0.601379232980797 1101 50-51 0.8466311256696812 1101 52-53 0.8699844041827802 1101 54-55 0.8363441935386291 1101 56-57 0.6971775877906552 1101 58-59 0.8027489620167998 1101 60-61 0.9571913035786395 1101 62-63 1.1643219587088893 1101 64-65 0.9494404307957041 1101 66-67 1.2021450105258182 1101 68-69 1.2033377940171661 1101 70-71 1.1657560662787425 1101 72-73 1.1819895361644654 1101 74-75 1.1602188067986567 1101 76-77 1.1592379772819505 1101 78-79 1.3816140922148428 1101 80-81 1.3953156760445182 1101 82-83 1.4108322884834692 1101 84-85 1.5244243186722208 1101 86-87 1.4048792170624012 1101 88-89 1.5856028412162786 1101 90-91 1.2649589802682648 1101 92-93 1.5956487565220243 1101 94-95 1.5854547419218257 1101 96-97 1.5932988877452487 1101 98-99 1.8663700778904975 1101 100-101 2.3982439022111777 1102 1 0.032372843462631806 1102 2 0.1082968165004985 1102 3 0.15212936988762493 1102 4 0.09460684485473791 1102 5 -0.06663073250756923 1102 6 -0.09707690040964678 1102 7 0.05536875610532377 1102 8 0.1770941546112894 1102 9 0.2853474205897939 1102 10-11 0.05746397559342853 1102 12-13 0.007305809921106743 1102 14-15 0.05249374302184151 1102 16-17 0.08430932983837636 1102 18-19 0.061336418122991176 1102 20-21 0.1824616565810686 1102 22-23 0.2948716896972883 1102 24-25 0.07276448441701433 1102 26-27 0.15985113846512888 1102 28-29 0.009824242664393523 1102 30-31 0.14845022760636084 1102 32-33 0.21525795131737624 1102 34-35 0.09635664592921955 1102 36-37 0.16360616359719415 1102 38-39 0.08729312968496572 1102 40-41 0.13146382940677626 1102 42-43 0.1675909144829646 1102 44-45 0.17597988461928082 1102 46-47 -0.12843436109891826 1102 48-49 -0.03583389090512412 1102 50-51 -0.10901448484725762 1102 52-53 -0.28619596569243555 1102 54-55 -0.39978548560576144 1102 56-57 -0.3587075503555113 1102 58-59 -0.12752343103132802 1102 60-61 -0.19566074633222996 1102 62-63 -0.1830905858543943 1102 64-65 0.055779557355421616 1102 66-67 0.1281699659625346 1102 68-69 0.07677807922215507 1102 70-71 0.14773578998640247 1102 72-73 0.13391689801829543 1102 74-75 -0.03576214239920361 1102 76-77 -0.011968573520189096 1102 78-79 0.11064149863195993 1102 80-81 0.035731785670186866 1102 82-83 -0.0030491731921102883 1102 84-85 -0.11143798079302059 1102 86-87 0.0022778356007258083 1102 88-89 0.18865542588650897 1102 90-91 0.484962322514253 1102 92-93 0.8364436540264784 1102 94-95 0.5870283827417886 1102 96-97 0.681478477763072 1102 98-99 0.9237870787817677 1102 100-101 1.7069699494482578 1103 1 0.09629329800808506 1103 2 0.15052305616991646 1103 3 0.06199507236696178 1103 4 0.12291262997870689 1103 5 -0.12213176556542038 1103 6 0.0835790913258947 1103 7 0.013271648667306124 1103 8 0.03931006370219592 1103 9 0.12212428009392085 1103 10-11 0.1164644921223541 1103 12-13 0.14960952892937485 1103 14-15 0.10078919756730187 1103 16-17 -0.034027447021124146 1103 18-19 -0.06101637113320635 1103 20-21 -0.059880802096614616 1103 22-23 -0.056677897079573825 1103 24-25 -0.005283036244144057 1103 26-27 -0.09384204335305668 1103 28-29 -0.11605385650915423 1103 30-31 0.032257045788185224 1103 32-33 -0.09327768917848545 1103 34-35 -0.0014739325831740757 1103 36-37 -0.07016191491521084 1103 38-39 -0.20773527940594505 1103 40-41 -0.06676705902297186 1103 42-43 0.18746436489617935 1103 44-45 -0.08174222281873256 1103 46-47 0.1598662587357964 1103 48-49 0.6769372867808272 1103 50-51 0.46328665151637693 1103 52-53 0.668284922737314 1103 54-55 0.35129922513804246 1103 56-57 0.613567759561839 1103 58-59 0.5155163416959354 1103 60-61 0.4519632206099118 1103 62-63 0.7011940215836248 1103 64-65 0.7617712928926039 1103 66-67 1.029048065136088 1103 68-69 0.6119020461643032 1103 70-71 0.8992595503169802 1103 72-73 0.9509106996711942 1103 74-75 0.6960689526421184 1103 76-77 0.8044312198682384 1103 78-79 0.6589111267311303 1103 80-81 0.8493294096371287 1103 82-83 0.9164239673037571 1103 84-85 0.8527149117689667 1103 86-87 1.039183827336263 1103 88-89 1.0603496407625386 1103 90-91 0.8216100497869832 1103 92-93 1.0494730961752374 1103 94-95 1.1338646430723678 1103 96-97 1.4429970728043955 1103 98-99 1.5333557771288717 1103 100-101 2.082731829613543 1104 1 0.062696460063421 1104 2 0.03535956353606906 1104 3 0.05319162285204726 1104 4 0.1524131689654098 1104 5 -0.13610948744828022 1104 6 -0.04452008222782666 1104 7 0.008061443852362515 1104 8 -0.05357531179187447 1104 9 0.01558457473998942 1104 10-11 -0.00895771412989177 1104 12-13 -0.067422450948456 1104 14-15 0.003951252903270586 1104 16-17 -0.00993474130786609 1104 18-19 0.2065006967791163 1104 20-21 0.18101032258897476 1104 22-23 0.1557906620292968 1104 24-25 -0.0051752389031420876 1104 26-27 0.023549260994776944 1104 28-29 -0.0767617257150448 1104 30-31 0.07415427898976645 1104 32-33 0.11974782285887642 1104 34-35 -0.05655837383362439 1104 36-37 -0.28077995300359504 1104 38-39 -0.07769575371424509 1104 40-41 -0.05106457968413025 1104 42-43 0.12716971883079253 1104 44-45 -0.08655717071668789 1104 46-47 0.0995177139998944 1104 48-49 0.290160427276696 1104 50-51 0.18146846969819563 1104 52-53 0.5041634710664553 1104 54-55 0.2885791389001682 1104 56-57 0.45147649114645816 1104 58-59 0.4981250373481103 1104 60-61 0.5466811509009659 1104 62-63 0.5797243845013398 1104 64-65 0.6145740237585784 1104 66-67 0.7182144323657056 1104 68-69 0.6874500159810495 1104 70-71 0.7284546635041877 1104 72-73 0.7664514829985372 1104 74-75 0.7715809900118664 1104 76-77 0.5262601814205219 1104 78-79 0.5976103721497417 1104 80-81 1.0000660248006241 1104 82-83 1.0939122892584834 1104 84-85 1.1210224935152802 1104 86-87 0.6419146933082374 1104 88-89 0.7553550306295875 1104 90-91 0.9303057019608971 1104 92-93 0.8695773002715406 1104 94-95 1.018198096180523 1104 96-97 1.4382719560239394 1104 98-99 1.7668268515090375 1104 100-101 2.3505902557723672 1105 1 -0.18609959645377216 1105 2 -0.0841829379404686 1105 3 -0.13212480984069686 1105 4 -0.08812565253293059 1105 5 -0.20516586834873607 1105 6 0.06702641724970704 1105 7 -0.09088908088526892 1105 8 -0.1647129248035526 1105 9 -0.1877455783652735 1105 10-11 -0.1719557605612252 1105 12-13 -0.07619079404963713 1105 14-15 -0.251091680175648 1105 16-17 -0.36798298991083556 1105 18-19 -0.3652815938937266 1105 20-21 -0.12800562398319926 1105 22-23 -0.21799403653050575 1105 24-25 -0.32715061485152575 1105 26-27 -0.46199773823288837 1105 28-29 -0.385584587011671 1105 30-31 -0.043343790157479134 1105 32-33 -0.41213396352065246 1105 34-35 -0.4562164993163407 1105 36-37 -0.5601970626305999 1105 38-39 -0.19953256258254015 1105 40-41 -0.2671042871031446 1105 42-43 -0.3319927453916449 1105 44-45 0.09011585640611486 1105 46-47 0.10293645917277416 1105 48-49 0.419732005124132 1105 50-51 0.07501601410780978 1105 52-53 -0.16006123476244483 1105 54-55 -0.09228156711046154 1105 56-57 0.14923556615440958 1105 58-59 0.3581495705360638 1105 60-61 0.5307629736259685 1105 62-63 0.5573662453890833 1105 64-65 0.4294733922561491 1105 66-67 0.8196864236427857 1105 68-69 0.5953256236317657 1105 70-71 0.5993260459947614 1105 72-73 0.7213319972868462 1105 74-75 0.5245908487188728 1105 76-77 0.8476676594996633 1105 78-79 0.65434904826623 1105 80-81 0.977191098627344 1105 82-83 0.6748831674557465 1105 84-85 0.940351465392979 1105 86-87 1.2361630236885013 1105 88-89 1.4018215700871366 1105 90-91 1.0628587436218844 1105 92-93 1.2956994664611727 1105 94-95 1.2317106580813935 1105 96-97 1.303702876927126 1105 98-99 1.0916701116971232 1105 100-101 1.5191025905140876 1106 1 -0.27219155047676225 1106 2 -0.07763121380253324 1106 3 -0.37442366041540964 1106 4 -0.050999215751325266 1106 5 -0.03574058099241739 1106 6 -0.07573220343994791 1106 7 -0.16353275904619125 1106 8 -0.1473566029644715 1106 9 -0.1355616703192979 1106 10-11 -0.15885231228535446 1106 12-13 -0.14682297795768307 1106 14-15 -0.21390777212966583 1106 16-17 -0.1262588519798058 1106 18-19 0.23793679691087277 1106 20-21 0.1106150656719791 1106 22-23 -0.20081012848452673 1106 24-25 -0.04962187921935168 1106 26-27 -0.047629922140934866 1106 28-29 -0.21627424218408464 1106 30-31 -0.1454702269390893 1106 32-33 0.022521208893138578 1106 34-35 -0.4231130510404739 1106 36-37 -0.3680131545846237 1106 38-39 -0.24303830970897167 1106 40-41 -0.40394336756291693 1106 42-43 -0.18946400975946176 1106 44-45 -0.1771829941685965 1106 46-47 -0.23102905806860718 1106 48-49 -0.3542335121172471 1106 50-51 -0.5388345606048333 1106 52-53 -0.29741755660152336 1106 54-55 -0.4559597280300025 1106 56-57 -0.21225868671915293 1106 58-59 -0.04690790072829998 1106 60-61 -0.3595243826958736 1106 62-63 -0.20700157070287872 1106 64-65 -0.028917412341556314 1106 66-67 -0.05042851888594413 1106 68-69 -0.28185828441420924 1106 70-71 0.08271685059246181 1106 72-73 -0.18619673834533756 1106 74-75 0.11602763032806962 1106 76-77 0.23634582041920282 1106 78-79 0.5047513471168052 1106 80-81 0.1929382250641254 1106 82-83 -0.0931628095557464 1106 84-85 0.15121353435849372 1106 86-87 0.3792664719643639 1106 88-89 0.7348675470986308 1106 90-91 0.5913070194839527 1106 92-93 0.49035463887496533 1106 94-95 0.5288370948630039 1106 96-97 0.8079557504903434 1106 98-99 1.2587965484787347 1106 100-101 2.078930176720988 1107 1 0.0012675690200936174 1107 2 -0.2105871886452988 1107 3 0.009601496817296606 1107 4 0.00365487229899486 1107 5 -0.2551116501748041 1107 6 -0.09717874432045193 1107 7 -0.1135956521279482 1107 8 -0.12295408724119739 1107 9 0.1840609711901351 1107 10-11 -0.017217092159576453 1107 12-13 0.012076393111499328 1107 14-15 -0.23180085389067528 1107 16-17 -0.13418338028169075 1107 18-19 0.07592421829451723 1107 20-21 -0.2816490852714253 1107 22-23 -0.20420635489962535 1107 24-25 -0.235565275445758 1107 26-27 -0.0864664001283515 1107 28-29 -0.3801736132532696 1107 30-31 -0.031036264674938252 1107 32-33 0.016514919584956544 1107 34-35 0.10842782946266993 1107 36-37 0.20217552466066024 1107 38-39 -0.07325843549514133 1107 40-41 0.3377861921855114 1107 42-43 0.08679385187576116 1107 44-45 -0.21186852875979412 1107 46-47 -0.08363912096169912 1107 48-49 0.03441428662489443 1107 50-51 0.048020785307123504 1107 52-53 0.05824282075695919 1107 54-55 -0.005928281489119058 1107 56-57 0.35991112460160224 1107 58-59 0.2535952439257869 1107 60-61 0.3417963720211148 1107 62-63 0.09221226577511032 1107 64-65 0.6349819587276286 1107 66-67 0.6869928647618551 1107 68-69 0.4989907721895648 1107 70-71 0.5051382342402597 1107 72-73 0.3924825069376787 1107 74-75 0.5444238567431618 1107 76-77 0.6010942480921599 1107 78-79 0.16374505780862947 1107 80-81 0.3540074074540627 1107 82-83 0.6366485111989704 1107 84-85 0.5121254840440308 1107 86-87 0.6896438304549264 1107 88-89 0.6257480502432813 1107 90-91 0.40891708237703384 1107 92-93 0.8474615571139559 1107 94-95 1.1579251451774688 1107 96-97 1.8668236750186473 1107 98-99 1.948387743447288 1107 100-101 2.2483641389851385 1108 1 -0.05399335227856028 1108 2 -0.07276634893766953 1108 3 -0.0974867234784682 1108 4 -0.01667489142699452 1108 5 -0.587272112523948 1108 6 -0.17924571695346003 1108 7 -0.08281203832547135 1108 8 -0.10933858494644966 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2316 64-65 0.5969159209917763 2316 66-67 0.493738147780725 2316 68-69 0.3131417155857932 2316 70-71 0.10188351725912881 2316 72-73 0.11630326165466087 2316 74-75 0.05102763032806834 2316 76-77 0.07717915375253526 2316 78-79 0.1730846804501418 2316 80-81 0.02877155839745882 2316 82-83 -0.26066280955574683 2316 84-85 -0.44711979897483545 2316 86-87 -0.8207335280356389 2316 88-89 -0.5842991195680369 2316 90-91 -0.23202631384938144 2316 92-93 -0.541312027791701 2316 94-95 -0.3978295718036655 2316 96-97 -0.6620442495096555 2316 98-99 -1.3445367848546006 2316 100-101 -1.5635698232790176 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 5.0 5 5.0 6 16.0 7 48.0 8 113.0 9 235.0 10 385.0 11 706.0 12 1071.0 13 1534.0 14 2133.0 15 3059.0 16 4375.0 17 6402.0 18 5586.0 19 3142.0 20 2459.0 21 2430.0 22 2426.0 23 2638.0 24 2861.0 25 3273.0 26 3727.0 27 4537.0 28 5553.0 29 6771.0 30 8418.0 31 10498.0 32 13558.0 33 17593.0 34 24549.0 35 35776.0 36 53261.0 37 76855.0 38 56666.0 39 4163.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.49307970273403 20.325356241903595 16.082634485579874 19.098929569782506 2 15.501961393666294 22.920636478378327 39.75481897658595 21.822583151369432 3 20.462283721931897 27.08304369041875 30.786656525757095 21.668016061892263 4 12.253087261129133 18.37118011067797 38.72856636589156 30.64716626230134 5 12.04410309261486 40.096843586157256 33.506735700439236 14.352317620788654 6 28.738029228718403 38.92855293036848 16.974574122688647 15.358843718224469 7 26.47813298221797 35.91081451676658 20.314259154760638 17.296793346254816 8 26.338013962833024 33.89462722616137 19.397215611548642 20.37014319945697 9 26.649056377459917 18.259361940512118 20.850473378749342 34.24110830327862 10-11 24.83582358088602 28.131346587393104 26.20389225556183 20.82893757615905 12-13 26.964619671004925 26.326148947422944 27.1619949075008 19.547236474071326 14-15 22.734992407948074 27.13238521280917 28.353247135601954 21.779375243640807 16-17 19.66804877450379 30.579067745092424 28.45969942562106 21.29318405478272 18-19 19.672546758589103 28.616038448222753 32.566222592965154 19.14519220022299 20-21 21.530516137562174 26.147771245847878 32.64614038363767 19.675572232952277 22-23 20.515962478430005 26.866741724345676 32.90470491700492 19.712590880219395 24-25 21.063356495805948 26.98191800539216 32.93809922334385 19.016626275458048 26-27 20.44220056184906 26.969027423195563 33.63365860241069 18.95511341254469 28-29 19.28196292727077 28.522474950213933 33.15854255294497 19.037019569570322 30-31 20.03554775298128 27.084579935419768 32.984171163521026 19.895701148077926 32-33 19.976365138169893 27.51566797789718 32.808568523379975 19.69939836055295 34-35 20.768282942281324 27.08140805984227 32.878355427976366 19.271953569900035 36-37 20.568463406854928 26.785222622951714 33.36645485250701 19.279859117686346 38-39 19.915165294100007 27.767691389222833 33.12887951127358 19.188263805403576 40-41 19.911348822754892 28.011945555310213 32.56908494647399 19.507620675460906 42-43 20.005970629410665 28.083905064266055 31.616391149836897 20.29373315648638 44-45 20.622439215878703 27.57455067865039 31.530595832958504 20.27241427251241 46-47 21.429213997726475 28.537528180551806 30.660849273916323 19.372408547805392 48-49 21.028893414133485 28.830306053741367 30.319956601268704 19.820843930856444 50-51 20.199628711859138 29.235669831611833 30.767983076675637 19.79671837985339 52-53 20.83793354432968 29.74857631988572 29.30368480308371 20.10980533270089 54-55 20.12697945375391 29.304775223468027 29.631628733667547 20.936616589110514 56-57 21.291821029302323 28.726307209586977 29.779653300838802 20.202218460271894 58-59 21.823809874301787 28.41485588731596 29.469156096404063 20.292178141978187 60-61 20.689772674610378 28.817221009129547 30.851263933527974 19.6417423827321 62-63 20.275004020925167 28.762018477173413 30.66752809877028 20.29544940313114 64-65 20.195675937965984 29.779516998290767 29.90000845075798 20.12479861298527 66-67 20.74770125752731 28.635938620236566 29.462340969002078 21.15401915323405 68-69 20.50726356278504 28.89968405069364 29.61990671453612 20.97314567198519 70-71 21.338845408376063 28.895322369156368 29.399096586711593 20.366735635755976 72-73 20.95147356684686 28.38268848597856 30.25534919349782 20.410488753676763 74-75 20.167325007973698 29.89223920551971 29.195324277392043 20.74511150911455 76-77 20.42861699256606 28.646025008791515 29.525312746196477 21.40004525244595 78-79 20.668373174568124 29.01145214008631 29.33598850696915 20.98418617837642 80-81 20.70572007273104 28.774694614141115 29.449664832034372 21.069920481093472 82-83 20.658178641089002 28.59222447424628 29.83135262743965 20.918244257225066 84-85 20.182618402777305 29.20885804206575 29.471513410144468 21.13701014501248 86-87 19.984134383408165 29.20541066594699 28.97751280562439 21.83294214502046 88-89 19.9401086603913 28.898184722665203 28.708587878341795 22.4531187386017 90-91 20.547818228164903 28.41817282146562 29.025314850988483 22.008694099381 92-93 20.95187986527507 29.037556413678047 28.876851547687366 21.133712173359523 94-95 20.14401727225889 29.10754543645439 28.685552747723065 22.06288454356366 96-97 19.70975735420398 29.329309682115195 29.10836325174263 21.852569711938195 98-99 20.407954638388354 29.735572335959436 27.62533053458005 22.23114249107216 100-101 19.901385967471274 29.037974718055747 28.049516949372038 23.01112236510094 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 585.0 1 393.0 2 249.0 3 307.5 4 274.0 5 259.5 6 436.0 7 846.0 8 1287.0 9 1464.5 10 1337.5 11 1191.5 12 1161.5 13 1250.5 14 1310.5 15 1292.0 16 1261.0 17 1263.0 18 1344.0 19 1374.0 20 1469.5 21 1616.0 22 1733.0 23 2020.0 24 2362.0 25 2725.5 26 3349.0 27 3962.5 28 4411.0 29 5014.5 30 5733.5 31 6315.0 32 6891.5 33 7661.0 34 8546.0 35 9101.0 36 9916.0 37 10733.0 38 11105.0 39 11108.5 40 11071.5 41 11461.0 42 11683.5 43 11929.0 44 12459.5 45 12848.5 46 12905.5 47 14974.5 48 19597.0 49 18128.0 50 12803.0 51 10731.0 52 9584.5 53 9004.5 54 8437.0 55 7591.0 56 6637.0 57 5804.5 58 5240.0 59 4601.0 60 3703.5 61 2918.0 62 2274.5 63 1643.0 64 1163.0 65 846.0 66 613.5 67 457.0 68 326.0 69 222.5 70 157.0 71 117.0 72 105.0 73 95.0 74 83.0 75 73.0 76 56.0 77 41.5 78 24.5 79 15.5 80 10.5 81 10.0 82 8.0 83 2.5 84 0.0 85 1.0 86 2.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04252639498842791 2 0.0 3 0.0 4 2.726050960796661E-4 5 0.01472067518830197 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0046342866333543235 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.3630254803983306E-4 22-23 5.452101921593322E-4 24-25 5.452101921593322E-4 26-27 0.0017719331245178297 28-29 0.00313495860491616 30-31 1.3630254803983306E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009677480910828146 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.3630254803983306E-4 84-85 1.3630254803983306E-4 86-87 0.0 88-89 0.0 90-91 0.008314455430429817 92-93 0.002589748412756828 94-95 0.0 96-97 0.0 98-99 2.726050960796661E-4 100-101 0.012948742063784142 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 366831.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.31775244112142 #Duplication Level Percentage of deduplicated Percentage of total 1 83.5768003126306 52.91895152016184 2 9.889426786746137 12.523525541355736 3 2.806818067685545 5.331642346709406 4 1.1976411702306549 3.033277885198383 5 0.636874960871511 2.016274555420561 6 0.39751774310797905 1.5101958029438594 7 0.2764452198487202 1.225272299573886 8 0.21647171691082678 1.09652020654914 9 0.14190057944502663 0.8086343184496692 >10 0.7910127555768074 8.670399749169322 >50 0.04059077858094255 1.7654515560500041 >100 0.021590839670651837 2.651321625984059 >500 0.0047499847275434045 2.3459151852060667 >1k 0.0021590839670651836 4.1026174072280925 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4255 1.1599346838189795 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3858 1.0517104606753518 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3277 0.8933268998530659 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2373 0.6468918929970477 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1242 0.3385755293309453 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 998 0.2720598858875068 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 887 0.24180072022266386 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 879 0.23961987945402652 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 861 0.2347129877245925 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 815 0.2221731533049279 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 795 0.21672105138333458 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 754 0.20554424244406824 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 736 0.20063735071463423 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 728 0.19845650994599692 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 602 0.164108267839959 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 525 0.1431176754418247 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 476 0.1297600257339211 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 395 0.1076790129514681 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 375 0.10222691102987479 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.3630254803983306E-4 0.0 24-25 0.0 0.0 0.0 5.452101921593322E-4 0.0 26-27 0.0 0.0 0.0 0.0010904203843186645 0.0 28-29 0.0 0.0 0.0 0.002044538220597496 0.0 30-31 0.0 0.0 0.0 0.006406219757872153 0.0 32-33 0.0 0.0 0.0 0.010495296199067146 0.0 34-35 0.0 0.0 0.0 0.014311767544182471 0.0 36-37 0.0 0.0 0.0 0.018673449081457127 0.0 38-39 0.0 0.0 0.0 0.02371664335893095 0.0 40-41 0.0 0.0 0.0 0.03284891407759977 0.0 42-43 0.0 0.0 0.0 0.05179496825513656 0.0 44-45 0.0 0.0 0.0 0.0771472421905455 0.0 46-47 0.0 0.0 0.0 0.10822422314362745 0.0 48-49 0.0 0.0 0.0 0.1686062519252735 0.0 50-51 0.0 0.0 0.0 0.2308965163794772 0.0 52-53 0.0 0.0 0.0 0.30340947193666834 0.0 54-55 0.0 0.0 0.0 0.3988212555645515 0.0 56-57 0.0 0.0 0.0 0.5114071602454536 0.0 58-59 0.0 0.0 0.0 0.656433071359836 0.0 60-61 0.0 0.0 0.0 0.812363186317405 0.0 62-63 0.0 0.0 0.0 1.018997849145792 0.0 64-65 0.0 0.0 0.0 1.2025973813554471 0.0 66-67 0.0 0.0 0.0 1.421362970959379 0.0 68-69 0.0 0.0 0.0 1.7238183250597685 0.0 70-71 0.0 0.0 0.0 2.026409981708198 0.0 72-73 0.0 0.0 0.0 2.378070555650967 0.0 74-75 0.0 0.0 0.0 2.7714397092939254 0.0 76-77 0.0 0.0 0.0 3.169715754666318 0.0 78-79 0.0 0.0 0.0 3.597978360607473 0.0 80-81 0.0 0.0 0.0 4.1111574539774445 0.0 82-83 0.0 0.0 0.0 4.712933203573307 0.0 84-85 0.0 0.0 0.0 5.314845255717211 0.0 86-87 0.0 0.0 0.0 5.908988062622843 0.0 88-89 0.0 0.0 0.0 6.666830229724314 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATGA 20 0.0020699871 71.27567 2 CCGCTCA 25 0.005019527 56.99722 9 GGACCGA 60 1.4899852E-6 47.49768 6 TAAGGTG 115 1.0298572E-7 33.055386 5 GGTACCT 60 0.00394052 31.678076 5 GTAAGGT 90 2.4025934E-5 31.678076 4 ACGGTGT 75 3.0752725E-4 31.665123 6 TATAGGT 95 3.4758727E-5 30.01081 3 AGGACCG 80 4.4782815E-4 29.698196 5 AAGGGTA 135 4.198646E-7 28.15829 4 AACGGTG 85 6.3853315E-4 27.951244 5 CGCTATA 35 0.0083916215 27.141535 44-45 CGGTGTC 70 0.008386248 27.141535 7 TAGTTTA 145 7.866365E-7 26.205618 9 TAAGGGT 165 8.348252E-8 25.918427 3 GCTAAGG 170 1.111639E-7 25.162983 1 CTAAGGG 170 1.11458576E-7 25.15612 2 GACCTAT 95 0.0012253108 24.998781 6 CTATTAG 100 0.0016454312 23.76504 1 AGGGTAG 165 2.4148176E-6 23.0386 5 >>END_MODULE