FastQCFastQC Report
Thu 26 May 2016
SRR937559_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937559_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062063
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70800.6666271209899978No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57920.54535371253871No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36950.3479077983132827No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14780.13916311932531308No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14160.13332542419799956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32600.053.6980861
GTATCAA59200.043.5518071
ATCAACG77250.032.869013
TCAACGC78550.032.266024
TATCAAC79500.031.9970572
CAACGCA79650.031.7040085
AACGCAG81400.031.0500286
TGGTATC14900.028.719682
CCCGAAC700.008348945527.1710133
CGCAGAG94650.026.7522818
ACGCAGA94700.026.7406837
GTGGTAT17950.025.4410691
GCAGAGT100050.024.7385859
GTACATG67350.024.6499731
TACATGG68650.023.4791582
TAAGGTG4950.023.057465
GAGTACT58350.022.71471812-13
ACATGGG68300.022.5563163
AGTACTT61250.021.40656312-13
GTACTTT62150.021.20972814-15
Produced by FastQC (version 0.11.5)