FastQCFastQC Report
Thu 26 May 2016
SRR937559_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937559_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062063
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78710.741104812049756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67090.6316951065991376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45120.4248335550715918No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16800.15818270667559267No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12180.1146824623398047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36250.056.9084241
GTATCAA64550.041.8996931
ATCAACG86100.031.458923
TATCAAC86850.031.1325362
TCAACGC87750.030.8673824
CAACGCA88450.030.6230955
AACGCAG90300.030.034196
GTAAGGT3850.028.3882854
CGATCGT1101.8189894E-1228.066524-25
ATGGGTA4100.027.8163055
TGGTATC15150.026.661122
ACGCAGA104000.026.0333187
CGCAGAG104050.026.020818
GTACATG69600.025.4055481
TACATGG70400.024.907252
TAAGGTG4600.024.7927955
GCAGAGT110500.024.072099
ACATGGG69150.024.051823
GGTAAGG4450.023.4927983
GAGTACT68850.023.21502512-13
Produced by FastQC (version 0.11.5)