FastQCFastQC Report
Thu 26 May 2016
SRR937558_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937558_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029972
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68080.6609888424151336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54020.5244802771337473No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36220.35166004512744037No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14600.14175142625236414No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13810.1340813148318595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32300.051.394971
GTATCAA59400.043.6423151
ATCAACG78300.033.032733
TCAACGC79550.032.5152554
TATCAAC80050.032.3121642
CAACGCA80500.032.1284075
GCGTAAA600.003923946531.7107451
AACGCAG83350.031.1196656
ACGCAGA92350.027.9312087
GTACATG68900.027.8216931
CGCAGAG92500.027.7845548
GTGTACG1056.840053E-527.1806371
GCTCGCG350.0083740427.155550-51
TACATGG69350.027.1504572
TGGTATC15950.025.9350472
ACATGGG69500.025.7907433
GCAGAGT97800.025.7445269
AGCGGTC1151.2805368E-424.7857078
CATGGGG27850.024.0725674
GAGTACT55150.023.7721912-13
Produced by FastQC (version 0.11.5)