##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937558_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1029972 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.702379287980644 33.0 31.0 34.0 30.0 34.0 2 31.822485465624307 33.0 31.0 34.0 30.0 34.0 3 31.85346591946189 33.0 31.0 34.0 30.0 34.0 4 35.455435681746685 37.0 35.0 37.0 33.0 37.0 5 35.44371788747655 37.0 35.0 37.0 33.0 37.0 6 35.53185329309923 37.0 35.0 37.0 33.0 37.0 7 35.46692628537475 37.0 35.0 37.0 33.0 37.0 8 35.45285405816857 37.0 35.0 37.0 33.0 37.0 9 37.05275871577091 39.0 37.0 39.0 33.0 39.0 10-11 36.94373536367979 39.0 37.0 39.0 33.0 39.0 12-13 36.81825331174051 39.0 37.0 39.0 33.0 39.0 14-15 37.93643565067788 40.0 38.0 41.0 33.0 41.0 16-17 37.87984285009689 40.0 38.0 41.0 33.0 41.0 18-19 37.86348026936655 40.0 38.0 41.0 33.0 41.0 20-21 37.71215091284035 40.0 38.0 41.0 32.0 41.0 22-23 37.57402094425868 40.0 37.0 41.0 32.0 41.0 24-25 37.42770968531184 40.0 37.0 41.0 32.0 41.0 26-27 37.50693271273394 40.0 37.0 41.0 32.0 41.0 28-29 37.43688614836132 40.0 37.0 41.0 32.0 41.0 30-31 37.18626477224623 39.5 37.0 41.0 31.5 41.0 32-33 36.91488021033582 39.0 36.0 41.0 31.0 41.0 34-35 36.62867776988112 39.0 35.0 41.0 30.0 41.0 36-37 36.63143221369124 39.0 35.0 41.0 30.0 41.0 38-39 36.56485953016198 39.0 35.0 41.0 30.0 41.0 40-41 36.34451858885484 39.0 35.0 41.0 30.0 41.0 42-43 36.05268201465671 39.0 35.0 41.0 29.5 41.0 44-45 35.7401463340751 39.0 35.0 41.0 28.0 41.0 46-47 35.28948456851254 39.0 35.0 40.5 26.0 41.0 48-49 34.879324874850965 39.0 35.0 40.0 24.0 41.0 50-51 33.95423176552372 38.0 33.5 39.5 22.5 40.5 52-53 34.032525641473754 38.0 33.5 39.5 23.0 40.5 54-55 34.30864091451029 38.0 34.0 40.0 22.5 41.0 56-57 34.045216763174146 38.0 34.0 40.0 20.5 41.0 58-59 33.81485710291153 38.0 34.0 40.0 19.5 41.0 60-61 33.51000658270322 38.0 33.0 40.0 18.5 41.0 62-63 33.14582677975712 37.0 33.0 40.0 17.0 41.0 64-65 32.77848524037547 36.5 32.5 39.5 13.5 41.0 66-67 32.3531722221575 36.0 32.0 39.0 8.0 41.0 68-69 31.88224437169166 35.5 31.0 39.0 7.5 40.0 70-71 31.432184078790492 35.0 31.0 38.5 4.0 40.0 72-73 30.979619348875502 35.0 31.0 37.5 2.0 40.0 74-75 30.492697374297556 35.0 30.0 37.0 2.0 39.0 76-77 30.048673167814272 34.5 30.0 36.5 2.0 39.0 78-79 29.529361477787745 34.0 29.0 36.0 2.0 38.5 80-81 29.019153433297216 34.0 29.0 35.5 2.0 37.0 82-83 28.51692570283464 34.0 28.0 35.0 2.0 37.0 84-85 28.052853378538444 34.0 27.0 35.0 2.0 36.5 86-87 27.544868695459684 33.5 26.0 35.0 2.0 36.0 88-89 27.057803513105213 33.0 25.5 35.0 2.0 35.5 90-91 26.563168222048752 33.0 25.0 35.0 2.0 35.0 92-93 26.027684247727123 32.5 24.0 35.0 2.0 35.0 94-95 25.396517089784965 32.0 21.0 34.5 2.0 35.0 96-97 24.634422586245066 32.0 14.0 34.0 2.0 35.0 98-99 23.72157932448649 31.0 2.0 34.0 2.0 35.0 100-101 21.261501283530038 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1653767804910693 1101 2 0.46251667514220784 1101 3 0.20447725188590482 1101 4 0.16496184583052553 1101 5 0.15914738350998192 1101 6 0.23319258720886182 1101 7 0.21761657225901132 1101 8 0.1755183348329794 1101 9 0.3283266503264457 1101 10-11 0.274887001587409 1101 12-13 0.4286865927808705 1101 14-15 0.589142834385413 1101 16-17 0.5238206909332845 1101 18-19 0.4658926475337921 1101 20-21 0.5890545554543749 1101 22-23 0.6504367379569871 1101 24-25 0.49773157350985286 1101 26-27 0.6586638815756629 1101 28-29 0.4333608839034042 1101 30-31 0.3025774101882561 1101 32-33 0.28915840784912206 1101 34-35 0.3945160368132008 1101 36-37 0.44380616405559437 1101 38-39 0.39428507310412186 1101 40-41 0.5018388964203027 1101 42-43 0.3288176393188351 1101 44-45 0.4980432731872355 1101 46-47 0.6250997042176394 1101 48-49 0.6927032739957824 1101 50-51 0.4351171305876065 1101 52-53 0.42934814998852033 1101 54-55 0.640011733487718 1101 56-57 0.6410809384278906 1101 58-59 0.7516898000616976 1101 60-61 0.7440270849605852 1101 62-63 0.607135936105891 1101 64-65 0.8220778821596468 1101 66-67 0.9045166337889228 1101 68-69 0.7540168320367115 1101 70-71 0.7098942603235656 1101 72-73 0.7074887187881096 1101 74-75 0.8457037429621117 1101 76-77 0.7957518555584322 1101 78-79 0.9023319120449784 1101 80-81 1.083289315922947 1101 82-83 1.0852718815680404 1101 84-85 0.9435331738752275 1101 86-87 0.9250301004047188 1101 88-89 0.9899921696102325 1101 90-91 1.032444194142105 1101 92-93 1.3091114926619696 1101 94-95 1.5949038815021872 1101 96-97 1.3350238702939095 1101 98-99 1.5428493809322141 1101 100-101 1.907300758033557 1102 1 0.17796737196960777 1102 2 0.2910286676395266 1102 3 0.26162996526215565 1102 4 0.1589054890826631 1102 5 0.101861013635407 1102 6 0.16165275981241223 1102 7 0.20057051527504655 1102 8 0.13503534952922536 1102 9 0.10852667212962785 1102 10-11 0.27183866273608004 1102 12-13 0.26308395131031403 1102 14-15 0.46718575977521937 1102 16-17 0.4569976685641137 1102 18-19 0.49010276749610426 1102 20-21 0.4274709424877585 1102 22-23 0.4641744605995939 1102 24-25 0.4567255358202189 1102 26-27 0.5767149010238057 1102 28-29 0.4002428196676462 1102 30-31 0.31514224105678323 1102 32-33 0.4416511388769848 1102 34-35 0.42135261897013265 1102 36-37 0.684403168059319 1102 38-39 0.6116400334029208 1102 40-41 0.7318878639798498 1102 42-43 0.8171686168033148 1102 44-45 0.7719050649011265 1102 46-47 0.7368947781916546 1102 48-49 0.8326651482929677 1102 50-51 0.7830977974364686 1102 52-53 0.9816500284970004 1102 54-55 0.9718475913608344 1102 56-57 1.0926218709171707 1102 58-59 1.0831224483868098 1102 60-61 1.1626616382268011 1102 62-63 1.20942758503881 1102 64-65 1.2661579446421953 1102 66-67 1.2388019896661504 1102 68-69 1.2041666915853853 1102 70-71 1.4005227744067739 1102 72-73 1.1970254310174546 1102 74-75 1.0839677317200334 1102 76-77 1.4999356772979944 1102 78-79 1.2904188619439516 1102 80-81 1.553054573297576 1102 82-83 1.3722641175441836 1102 84-85 1.497344474793497 1102 86-87 1.3250789335610875 1102 88-89 1.3171843975221904 1102 90-91 1.655938691825142 1102 92-93 1.7975782062204146 1102 94-95 1.7722951754613625 1102 96-97 2.0062927259404084 1102 98-99 2.357137409939405 1102 100-101 2.195675106738136 1103 1 0.17126617328522187 1103 2 0.29635391374135267 1103 3 0.13002524886247357 1103 4 0.13063329959722125 1103 5 0.08012873901514439 1103 6 0.08954809237388872 1103 7 0.004244186267577277 1103 8 0.15778716829228756 1103 9 0.14471616909691676 1103 10-11 0.3091683067020483 1103 12-13 0.2376907499993095 1103 14-15 0.3436149936194681 1103 16-17 0.42962190030592495 1103 18-19 0.2792587347094013 1103 20-21 0.41684493086380314 1103 22-23 0.25071388585318033 1103 24-25 0.3301515785489073 1103 26-27 0.07348814461420972 1103 28-29 0.244916061530013 1103 30-31 0.17548305392723051 1103 32-33 0.2821194811673351 1103 34-35 0.34341689345214377 1103 36-37 0.37631435251616807 1103 38-39 0.4746737100403777 1103 40-41 0.3406521995193188 1103 42-43 0.29332381215833436 1103 44-45 0.2728133664097143 1103 46-47 0.2530444006147192 1103 48-49 0.45243494444174104 1103 50-51 0.20965101821673926 1103 52-53 0.21751897387363073 1103 54-55 0.3429123381740382 1103 56-57 0.4014267434165575 1103 58-59 0.3980427857003903 1103 60-61 0.29764575465068077 1103 62-63 0.2708421593359276 1103 64-65 0.418134823454686 1103 66-67 0.710733565005853 1103 68-69 0.6627469907668697 1103 70-71 0.6221202618352777 1103 72-73 0.42815198409423516 1103 74-75 0.6828793539472464 1103 76-77 0.8100344204362351 1103 78-79 0.601264262763042 1103 80-81 0.594186267296088 1103 82-83 0.4870103547350837 1103 84-85 0.6428592005822047 1103 86-87 0.5531007731182527 1103 88-89 0.5201320033214962 1103 90-91 0.7452024455908344 1103 92-93 0.8233940575397725 1103 94-95 0.6362713544172784 1103 96-97 0.6389259866960231 1103 98-99 0.7909282421063111 1103 100-101 1.0146325796510958 1104 1 0.01182482544098562 1104 2 0.14864314404021428 1104 3 0.1882658311801002 1104 4 0.03289783548936498 1104 5 0.15348215125298736 1104 6 0.15247693720482403 1104 7 0.06226207795320704 1104 8 0.155976980085633 1104 9 0.2015970891399519 1104 10-11 0.18784417203521997 1104 12-13 0.1508630846202763 1104 14-15 0.32981993367673823 1104 16-17 0.23121483652968067 1104 18-19 0.29038591678361314 1104 20-21 0.4308575367704677 1104 22-23 0.37459602297769123 1104 24-25 0.22545096883224858 1104 26-27 0.24048811227629585 1104 28-29 0.12099607434758042 1104 30-31 0.23852324407416603 1104 32-33 0.14016081784028955 1104 34-35 0.2176088130894911 1104 36-37 0.1876013132814549 1104 38-39 0.17953329000027907 1104 40-41 0.3252784614329016 1104 42-43 0.34828430110759 1104 44-45 0.4343313227711647 1104 46-47 0.45129528696752885 1104 48-49 0.36695775443003953 1104 50-51 0.2096385240228642 1104 52-53 0.37839421420311936 1104 54-55 0.4162609732200977 1104 56-57 0.6917936464439691 1104 58-59 0.7451469255412988 1104 60-61 0.8615884665669071 1104 62-63 0.797476840866274 1104 64-65 0.6889574999756007 1104 66-67 0.5684989269846952 1104 68-69 0.8783188720690873 1104 70-71 0.7132051400389372 1104 72-73 0.6507533193560846 1104 74-75 0.6454511588143959 1104 76-77 0.3788858806400235 1104 78-79 0.5886557837621069 1104 80-81 0.6449994188315493 1104 82-83 0.9201634776955885 1104 84-85 0.9577606581401632 1104 86-87 0.7166413189822336 1104 88-89 0.7978228117105388 1104 90-91 0.9491764076907998 1104 92-93 1.1464332775492991 1104 94-95 0.9098688444072245 1104 96-97 1.2577086553382415 1104 98-99 1.4286693800480919 1104 100-101 1.3323658095031057 1105 1 0.2793716656993901 1105 2 0.25069412071901453 1105 3 0.2480740322333368 1105 4 0.35135677578178814 1105 5 0.3761069478486334 1105 6 0.1879164145072636 1105 7 0.29068469644210637 1105 8 0.22672677715060274 1105 9 0.3555754041897359 1105 10-11 0.44420874772641383 1105 12-13 0.39281946091026043 1105 14-15 0.3777719231158585 1105 16-17 0.3208869641072951 1105 18-19 0.33352979152483186 1105 20-21 0.4704968868677497 1105 22-23 0.3041589823974462 1105 24-25 0.34108188464180955 1105 26-27 0.3922944029994113 1105 28-29 0.3876575847248702 1105 30-31 0.24916897920959258 1105 32-33 0.4058949470443878 1105 34-35 0.5196024197912692 1105 36-37 0.5111845038781908 1105 38-39 0.4874572552568992 1105 40-41 0.4746690556101498 1105 42-43 0.3590896058780828 1105 44-45 0.4260397090753614 1105 46-47 0.4366790838537824 1105 48-49 0.4886996240755437 1105 50-51 0.6547394358600727 1105 52-53 0.5470638239596255 1105 54-55 0.6501558915467314 1105 56-57 0.9522458329985213 1105 58-59 0.9120346117965994 1105 60-61 0.8294278636715262 1105 62-63 0.8404799188706704 1105 64-65 0.8411786717498728 1105 66-67 0.8999220553455203 1105 68-69 0.9932566924755335 1105 70-71 1.0730613246200846 1105 72-73 1.0383190526592792 1105 74-75 1.1234294228018946 1105 76-77 1.1038077290646378 1105 78-79 1.252908130562119 1105 80-81 1.0767081205204363 1105 82-83 1.1130542562226964 1105 84-85 1.322498171722355 1105 86-87 1.1716663357464299 1105 88-89 1.2628823180433884 1105 90-91 1.1666641884113318 1105 92-93 1.0527099585309259 1105 94-95 1.3732646964978095 1105 96-97 1.1608194747602312 1105 98-99 1.4864407216420013 1105 100-101 1.3977289111463342 1106 1 0.13927448772210838 1106 2 0.29408634004520806 1106 3 0.38542963283828513 1106 4 0.17796890050424707 1106 5 0.15958830061756402 1106 6 0.21604091172385154 1106 7 0.24874532005442518 1106 8 0.2402890579370336 1106 9 0.3745347632012255 1106 10-11 0.10486936490310228 1106 12-13 0.014268603362872057 1106 14-15 0.2611225521984579 1106 16-17 0.25179688140946865 1106 18-19 0.16904520485301333 1106 20-21 0.32254220977537074 1106 22-23 0.1650090483626343 1106 24-25 0.3088647304763441 1106 26-27 0.3578702307820123 1106 28-29 0.6215222595647774 1106 30-31 0.7319645354087143 1106 32-33 0.44220073589144704 1106 34-35 0.19766859413242344 1106 36-37 0.26402221343831656 1106 38-39 0.306211880864268 1106 40-41 0.4116272562086607 1106 42-43 0.37917037300489653 1106 44-45 0.33651329082392323 1106 46-47 0.5621146953992948 1106 48-49 0.9401239089164193 1106 50-51 0.7536136032507486 1106 52-53 0.7687000018403651 1106 54-55 0.884456177932158 1106 56-57 0.733475118322076 1106 58-59 0.5203405937124899 1106 60-61 0.4685641219976233 1106 62-63 0.6674974881341171 1106 64-65 0.4350849368333769 1106 66-67 0.5534584327307215 1106 68-69 0.5808025463224311 1106 70-71 0.5882011386304384 1106 72-73 0.5568934806928745 1106 74-75 0.5731949246199584 1106 76-77 0.7068409208141624 1106 78-79 0.7485620707751366 1106 80-81 0.46267644114340456 1106 82-83 0.3677454265415818 1106 84-85 0.49404022854895047 1106 86-87 0.43878671416133486 1106 88-89 0.6366852913033547 1106 90-91 0.9032334357646832 1106 92-93 0.9224492351663827 1106 94-95 0.9988941813258201 1106 96-97 0.7646534999235364 1106 98-99 1.0943484990979968 1106 100-101 1.1194700702028726 1107 1 0.5050326987633404 1107 2 0.5463758688987888 1107 3 0.5665984878899586 1107 4 0.3890607023358683 1107 5 0.5149801870422195 1107 6 0.5236496873316341 1107 7 0.4434545774747036 1107 8 0.29582346523410763 1107 9 0.5707391105475779 1107 10-11 0.5298408607286262 1107 12-13 0.6392391796988974 1107 14-15 0.6901163548392333 1107 16-17 0.6798895843408062 1107 18-19 0.8156738966033501 1107 20-21 0.8118131465512661 1107 22-23 0.8330609259601829 1107 24-25 0.7912698391600088 1107 26-27 0.811715535868629 1107 28-29 0.7795710108980387 1107 30-31 0.6887030142052808 1107 32-33 0.9147383430119689 1107 34-35 1.0561053700344445 1107 36-37 1.0044894060529757 1107 38-39 0.9868938662259978 1107 40-41 1.0002678829327039 1107 42-43 0.9197069870767649 1107 44-45 0.8984910207882493 1107 46-47 1.1428729983894712 1107 48-49 0.9673771179347739 1107 50-51 1.0376213821319098 1107 52-53 0.9374488524784965 1107 54-55 0.8833178499318635 1107 56-57 1.2633047369178314 1107 58-59 1.2105820516550452 1107 60-61 1.3537221822425707 1107 62-63 1.2119888265430276 1107 64-65 1.2566478850845044 1107 66-67 1.2077248640080782 1107 68-69 1.3235381195321807 1107 70-71 1.2800985971614622 1107 72-73 1.1176887471609298 1107 74-75 1.1591796059249013 1107 76-77 1.0249893448091605 1107 78-79 1.2474058127296033 1107 80-81 1.1603101349166245 1107 82-83 1.2324099048232426 1107 84-85 1.2847933823979325 1107 86-87 1.4587911277109313 1107 88-89 1.3008886456010522 1107 90-91 1.4300213830963244 1107 92-93 1.4211219520739533 1107 94-95 1.802833734086125 1107 96-97 1.636073927302256 1107 98-99 2.1892159087121783 1107 100-101 2.536758311771152 1108 1 0.2096849704069612 1108 2 0.16632861842466795 1108 3 0.12661160903831004 1108 4 0.26285658602370177 1108 5 0.16293099349093865 1108 6 0.10731314063146158 1108 7 0.11172165378887655 1108 8 0.22640685443389685 1108 9 0.19802052332958198 1108 10-11 0.28414466908815683 1108 12-13 0.14079955669888733 1108 14-15 0.295741150941609 1108 16-17 0.2891899152375217 1108 18-19 0.25056512730254354 1108 20-21 0.36190743239870926 1108 22-23 0.2300294387891597 1108 24-25 0.10886974451565834 1108 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92-93 -0.0809215943408148 2316 94-95 -0.07705087530105814 2316 96-97 -0.07582706328430078 2316 98-99 -0.04402003472508298 2316 100-101 0.6925799569491247 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4473.0 3 921.0 4 918.0 5 1052.0 6 1131.0 7 1289.0 8 1631.0 9 1977.0 10 2478.0 11 3190.0 12 4358.0 13 5698.0 14 6555.0 15 8494.0 16 11110.0 17 14707.0 18 12348.0 19 7993.0 20 7425.0 21 7774.0 22 8285.0 23 8859.0 24 9914.0 25 11023.0 26 13041.0 27 14831.0 28 17803.0 29 21277.0 30 26079.0 31 32279.0 32 41121.0 33 53539.0 34 72134.0 35 101326.0 36 143833.0 37 189455.0 38 141687.0 39 17961.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.29499673215028 19.029209998548705 14.946881614230097 21.728911655070917 2 15.242478262097139 20.071987483485426 41.30128524042398 23.384249013993454 3 19.044726310184064 25.638554404533476 30.00614289483189 25.310576390450574 4 12.213282655596489 16.640802068648604 37.382100270263955 33.763815005490954 5 12.96761285360309 38.240102034358706 33.82575296586035 14.96653214617785 6 30.879601476948228 36.56760346177983 17.686990109895458 14.865804951376491 7 28.28756188606446 33.92090841786677 20.207764007483238 17.58376568858553 8 26.86653124440605 34.57995750542857 19.385326920233176 19.16818432993221 9 27.19293636894338 16.706168515275348 20.52967503405332 35.57122008172796 10-11 24.921335764449022 27.240155051732557 26.512717627988707 21.325791555829714 12-13 26.062776892133744 24.92268789747566 27.60590554034799 21.4086296700426 14-15 23.00860330656911 25.679252580140638 26.713546620094814 24.598597493195435 16-17 21.293054557092674 29.327148970445606 27.315415153569806 22.06438131889191 18-19 21.364557917634652 26.880275277554023 30.922173968876592 20.832992835934732 20-21 22.266551754661574 25.73278289704708 31.22754814290981 20.77311720538154 22-23 21.967611655056782 25.782700500957507 31.172991585528802 21.076696258456913 24-25 21.440658798121472 26.022185308860262 31.421372512300177 21.115783380718092 26-27 20.98354738978249 26.476765746817364 31.16345403639576 21.376232827004387 28-29 21.044136304552566 26.518511095371156 31.359017567346687 21.078335032729594 30-31 21.05371458592777 26.212640099626398 31.34476183063512 21.388883483810712 32-33 20.847325782154172 26.28960392065862 31.25678338837881 21.606286908808396 34-35 21.598516519385555 26.396819389543058 31.004092997734112 21.00057109333728 36-37 21.542173011121136 26.030656689340198 30.920308658338175 21.50686164120049 38-39 21.1209233811335 26.471487923029525 30.852724035619854 21.55486466021712 40-41 21.41622623672145 26.352753748922837 30.90631631980174 21.32470369455397 42-43 21.184580050642367 26.339381688396713 30.721269502807896 21.754768758153023 44-45 21.326276099831123 26.664678941920823 30.56438088048748 21.44466407776057 46-47 21.671858097854425 27.007002108998652 29.652200051363064 21.668939741783856 48-49 21.591772024123124 27.128006573196128 29.39992342946487 21.880297973215878 50-51 21.86553394681425 27.364777270624472 29.08988223481917 21.67980654774211 52-53 21.99610366356828 27.276641832874027 29.067166067230293 21.660088436327403 54-55 21.614697587725484 27.383017283897775 29.184951422781825 21.817333705594915 56-57 21.757889054772335 27.82014559919857 28.76498421945894 21.65698112657015 58-59 21.850388052675374 27.15498229094784 29.00559404715184 21.98903560922495 60-61 21.627329132142997 27.391420624732564 29.41382697319796 21.56742326992648 62-63 21.534751564740706 27.371328007873913 29.494541703968714 21.59937872341667 64-65 21.49941865469256 27.211434145563416 29.38354056068822 21.905606639055804 66-67 21.463517583150207 27.63494387601031 28.967788335994094 21.93375020484539 68-69 21.573970873427594 27.327071917774916 29.230054016623193 21.8689031921743 70-71 21.56920910273942 27.481243447120818 29.112047182675305 21.837500267464456 72-73 21.587240916831764 27.459776520555966 28.87789549238324 22.07508707022903 74-75 21.491131224800473 27.96220009824473 28.680262050785714 21.866406626169088 76-77 21.578240410222094 27.664216253649844 28.78830026669883 21.969243069429233 78-79 21.678360110070432 27.693715110561644 28.677258043963977 21.950666735403946 80-81 21.576941463222482 27.990071473138368 28.56698229876326 21.86600476487589 82-83 21.773537550147008 28.028694468768705 28.332967861669868 21.864800119414422 84-85 21.664572705508025 27.944316840309853 28.29730812541207 22.093802328770053 86-87 21.63809823882729 27.75088116982983 28.44390440528998 22.16711618605289 88-89 21.71715575412214 27.83434321233425 28.297650833909955 22.150850199633652 90-91 21.629365518187136 27.828781868641407 28.472488670575498 22.069363942595963 92-93 21.72300324972927 27.416521150957536 28.607829207105933 22.25264639220726 94-95 21.516652021880137 27.526201362816117 28.577124859650755 22.380021755652994 96-97 21.72276557503326 27.457001859589337 28.31103187756277 22.50920068781463 98-99 21.44167488585541 27.502961826002203 28.364031273769992 22.691332014372396 100-101 21.807247428234668 26.988170560215973 28.8185050404461 22.386076971103257 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1374.0 1 820.5 2 330.5 3 400.0 4 328.5 5 301.0 6 504.0 7 1067.0 8 1854.5 9 2267.0 10 2120.0 11 1843.5 12 1821.0 13 1948.5 14 2080.0 15 2156.0 16 2188.5 17 2308.5 18 2433.5 19 2562.0 20 2780.5 21 3140.5 22 3604.0 23 4314.0 24 5128.5 25 6077.0 26 7667.0 27 9382.0 28 10353.0 29 11397.5 30 13119.5 31 15316.0 32 17989.0 33 20170.5 34 22176.5 35 24018.5 36 26931.5 37 29935.5 38 30682.5 39 30697.0 40 31023.0 41 32299.0 42 33381.0 43 34198.5 44 35673.5 45 37327.0 46 37591.0 47 37477.0 48 37667.5 49 36855.0 50 35804.5 51 33850.5 52 31704.5 53 31133.5 54 30756.0 55 28428.5 56 25229.5 57 22555.0 58 20888.0 59 18729.0 60 15320.5 61 12764.0 62 10760.0 63 8184.0 64 6107.5 65 4868.5 66 3605.5 67 2717.0 68 2056.0 69 1458.0 70 1073.0 71 772.0 72 629.0 73 569.0 74 450.5 75 338.0 76 244.0 77 162.0 78 119.0 79 83.5 80 48.0 81 37.0 82 33.5 83 24.0 84 17.5 85 15.0 86 7.5 87 6.5 88 8.0 89 5.0 90 2.5 91 2.5 92 4.0 93 3.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3206883293914786 2 0.27660946122807223 3 0.2688422597895865 4 0.27457057085046976 5 0.2783570815517315 6 0.21136496914479228 7 0.201461787310723 8 0.2015588773287041 9 0.21087951905488694 10-11 0.21223877930662194 12-13 0.2094717137941614 14-15 0.21063679400993426 16-17 0.19447130601608587 18-19 0.20039379711293123 20-21 0.1882575448652973 22-23 0.19811218169037606 24-25 0.19801509167239498 26-27 0.19903453686119624 28-29 0.20932607876718978 30-31 0.20699591833564407 32-33 0.21219023429763137 34-35 0.20573374810189016 36-37 0.19170424050362533 38-39 0.17840290804021858 40-41 0.1757329325457391 42-43 0.19214114558454015 44-45 0.19379167589021837 46-47 0.19379167589021837 48-49 0.20986007386608568 50-51 0.20597647314684284 52-53 0.2271906420757069 54-55 0.19621892633974514 56-57 0.17544166249179588 58-59 0.17723782782444572 60-61 0.1642763104239727 62-63 0.1715095167635625 64-65 0.17024734652980858 66-67 0.17063570660173286 68-69 0.17184933182649625 70-71 0.17456785232996624 72-73 0.1727231419883259 74-75 0.18709246464952445 76-77 0.17922817319305767 78-79 0.18476230421797876 80-81 0.17704364778848355 82-83 0.15733437413832607 84-85 0.15709164909337342 86-87 0.1603441646957393 88-89 0.16636374581056573 90-91 0.17345131712318393 92-93 0.1682084561522061 94-95 0.16830554617018714 96-97 0.17359695215015555 98-99 0.1835001339842248 100-101 0.16408213038801056 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1029972.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.28820773961244 #Duplication Level Percentage of deduplicated Percentage of total 1 79.05168559069226 42.91574329514028 2 11.643603811982233 12.642207651652695 3 3.719705357322415 6.058084115054058 4 1.6290601965219573 3.537550334764715 5 0.8961994592612534 2.4326531210251625 6 0.5579433916252328 1.8173848050896744 7 0.36620980139131654 1.39166116419498 8 0.2860032970932103 1.2421285125448234 9 0.2223829877279568 1.08654964519779 >10 1.4493826644861434 14.923740856722336 >50 0.1253089126063314 4.674278392167389 >100 0.05036376376275785 5.010028986208489 >500 0.0012546132240595547 0.451087458124383 >1k 5.376913817398091E-4 0.6288563938433751 >5k 3.584609211598727E-4 1.1880452682697833 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6808 0.6609888424151336 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5402 0.5244802771337473 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3622 0.35166004512744037 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1460 0.14175142625236414 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1381 0.1340813148318595 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.709001798107132E-5 0.0 0.0 0.0 0.0 8 9.709001798107132E-5 0.0 0.0 0.0 0.0 9 9.709001798107132E-5 0.0 0.0 0.0 0.0 10-11 9.709001798107132E-5 0.0 0.0 0.0 0.0 12-13 9.709001798107132E-5 0.0 0.0 0.0 0.0 14-15 1.9418003596214265E-4 0.0 0.0 0.0 0.0 16-17 1.9418003596214265E-4 0.0 0.0 0.0 0.0 18-19 1.9418003596214265E-4 0.0 0.0 0.0 0.0 20-21 1.9418003596214265E-4 0.0 0.0 0.0 0.0 22-23 1.9418003596214265E-4 0.0 0.0 0.0 0.0 24-25 1.9418003596214265E-4 0.0 0.0 9.709001798107132E-5 0.0 26-27 1.9418003596214265E-4 0.0 0.0 1.9418003596214265E-4 0.0 28-29 3.398150629337496E-4 0.0 0.0 5.339950988958923E-4 0.0 30-31 3.883600719242853E-4 0.0 0.0 0.0011165352067823203 0.0 32-33 3.883600719242853E-4 0.0 0.0 0.0016019852966876768 0.0 34-35 4.36905080914821E-4 0.0 0.0 0.002621430485488926 0.0 36-37 4.8545008990535666E-4 0.0 0.0 0.0036894206832807103 0.0 38-39 4.8545008990535666E-4 0.0 0.0 0.004805955890063031 0.0 40-41 5.339950988958923E-4 0.0 0.0 0.007670111420504635 0.0 42-43 6.796301258674993E-4 0.0 0.0 0.011699347166719096 0.0 44-45 6.796301258674993E-4 0.0 0.0 0.020534538802996584 0.0 46-47 7.767201438485706E-4 0.0 0.0 0.03514658650914782 0.0 48-49 7.767201438485706E-4 0.0 0.0 0.04869064401750727 0.0 50-51 7.767201438485706E-4 0.0 0.0 0.06708920242492028 0.0 52-53 7.767201438485706E-4 0.0 0.0 0.0913131619111976 0.0 54-55 7.767201438485706E-4 0.0 0.0 0.11747892175709632 0.0 56-57 7.767201438485706E-4 0.0 0.0 0.1481108224301243 0.0 58-59 7.767201438485706E-4 0.0 0.0 0.18723809967649607 0.0 60-61 7.767201438485706E-4 0.0 0.0 0.23903562426939762 0.0 62-63 8.73810161829642E-4 0.0 0.0 0.3013188708042549 0.0 64-65 8.73810161829642E-4 0.0 0.0 0.36059232678169895 0.0 66-67 8.73810161829642E-4 0.0 0.0 0.4373419859957358 0.0 68-69 8.73810161829642E-4 0.0 0.0 0.5318591185003088 0.0 70-71 8.73810161829642E-4 0.0 0.0 0.6183663245214432 0.0 72-73 8.73810161829642E-4 0.0 0.0 0.7324470956492022 0.0 74-75 8.73810161829642E-4 0.0 0.0 0.8343916145293271 0.0 76-77 8.73810161829642E-4 0.0 0.0 0.9485209306660763 0.0 78-79 8.73810161829642E-4 0.0 0.0 1.0838158707226992 0.0 80-81 9.709001798107133E-4 0.0 0.0 1.2244022167592905 0.0 82-83 9.709001798107133E-4 0.0 0.0 1.3870765418865756 0.0 84-85 0.0010679901977917847 0.0 0.0 1.5819362079745858 0.0 86-87 0.001165080215772856 0.0 0.0 1.8100006602121224 0.0 88-89 0.001165080215772856 0.0 0.0 2.086949936503128 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3230 0.0 51.39497 1 GTATCAA 5940 0.0 43.642315 1 ATCAACG 7830 0.0 33.03273 3 TCAACGC 7955 0.0 32.515255 4 TATCAAC 8005 0.0 32.312164 2 CAACGCA 8050 0.0 32.128407 5 GCGTAAA 60 0.0039239465 31.710745 1 AACGCAG 8335 0.0 31.119665 6 ACGCAGA 9235 0.0 27.931208 7 GTACATG 6890 0.0 27.821693 1 CGCAGAG 9250 0.0 27.784554 8 GTGTACG 105 6.840053E-5 27.180637 1 GCTCGCG 35 0.00837404 27.1555 50-51 TACATGG 6935 0.0 27.150457 2 TGGTATC 1595 0.0 25.935047 2 ACATGGG 6950 0.0 25.790743 3 GCAGAGT 9780 0.0 25.744526 9 AGCGGTC 115 1.2805368E-4 24.785707 8 CATGGGG 2785 0.0 24.072567 4 GAGTACT 5515 0.0 23.77219 12-13 >>END_MODULE