FastQCFastQC Report
Thu 26 May 2016
SRR937558_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937558_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029972
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76160.7394375769438393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64760.6287549564454179No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45410.4408857716520449No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17610.17097552166466662No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11600.11262442085804275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37500.052.2004051
GTATCAA65350.040.569211
ATCAACG87000.030.5237033
TCAACGC88400.030.0402954
TATCAAC89550.029.6545182
CAACGCA89750.029.534075
AACGCAG92450.028.7187296
ATATGCG350.00839423727.14209688-89
TGGTATC18300.025.9593352
ACGCAGA105650.025.047977
CGCAGAG106050.024.9534938
TAAGGTG5300.024.1997815
ATGGGCG1401.778298E-523.7516385
GTACATG69550.023.5683881
GCAGAGT111700.023.3510289
GGACCGA2853.6379788E-1223.3315456
TACATGG70250.023.2625222
GAGTACT66150.022.94710212-13
GTATAGG3150.022.6250041
GTAAGGT4850.022.5284234
Produced by FastQC (version 0.11.5)