##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937558_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1029972 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.214060188043945 33.0 31.0 34.0 31.0 34.0 2 32.42768055830644 34.0 31.0 34.0 31.0 34.0 3 32.49669311398756 34.0 31.0 34.0 31.0 34.0 4 36.13259875025729 37.0 35.0 37.0 35.0 37.0 5 36.0517111144769 37.0 35.0 37.0 35.0 37.0 6 36.09598610447663 37.0 35.0 37.0 35.0 37.0 7 36.043360402030345 37.0 35.0 37.0 35.0 37.0 8 36.026540527315305 37.0 35.0 37.0 35.0 37.0 9 37.69773741422097 39.0 37.0 39.0 35.0 39.0 10-11 37.62581312890059 39.0 37.0 39.0 35.0 39.0 12-13 37.52667985149111 39.0 37.0 39.0 35.0 39.0 14-15 38.7080032272722 40.0 38.0 41.0 34.5 41.0 16-17 38.67254109820461 40.0 38.0 41.0 34.5 41.0 18-19 38.699413673381414 40.0 38.0 41.0 34.5 41.0 20-21 38.613243369722674 40.0 38.0 41.0 34.0 41.0 22-23 38.459750847595856 40.0 38.0 41.0 34.0 41.0 24-25 38.51278190086721 40.0 38.0 41.0 34.0 41.0 26-27 38.38293176901897 40.0 38.0 41.0 34.0 41.0 28-29 38.23207426998015 40.0 38.0 41.0 34.0 41.0 30-31 38.22153951757912 40.0 37.5 41.0 34.0 41.0 32-33 38.1038596194848 40.0 37.0 41.0 34.0 41.0 34-35 37.94954086130497 40.0 37.0 41.0 33.0 41.0 36-37 37.75315396923411 40.0 37.0 41.0 33.0 41.0 38-39 37.592688927465986 40.0 37.0 41.0 33.0 41.0 40-41 37.38426384406566 40.0 36.0 41.0 33.0 41.0 42-43 37.1130977346957 40.0 36.0 41.0 32.0 41.0 44-45 36.72588817948449 39.5 36.0 41.0 31.0 41.0 46-47 36.27282197962663 39.0 35.5 41.0 30.0 41.0 48-49 35.85057312237615 39.0 35.0 41.0 28.5 41.0 50-51 35.558426831020654 39.0 35.0 40.5 27.5 41.0 52-53 35.28366499283476 39.0 35.0 40.0 27.0 41.0 54-55 35.040110313678426 38.5 35.0 40.0 26.0 41.0 56-57 34.76359260251735 38.0 34.0 40.0 26.0 41.0 58-59 34.48653992535719 38.0 34.0 40.0 25.5 41.0 60-61 34.128050082914875 38.0 34.0 40.0 24.5 41.0 62-63 33.76857332043978 37.0 33.5 40.0 23.0 41.0 64-65 33.37404609057334 36.5 33.0 39.5 22.0 41.0 66-67 32.93312925011554 36.0 32.5 39.0 21.5 40.0 68-69 32.4798504231183 35.5 32.0 39.0 19.5 40.0 70-71 32.062236157876136 35.0 31.5 38.0 18.5 40.0 72-73 31.568456229878095 35.0 31.0 37.5 17.0 39.5 74-75 31.058178280574616 35.0 31.0 37.0 12.5 39.0 76-77 29.858255370048894 33.5 29.5 35.5 11.0 38.0 78-79 30.4473558504503 34.0 30.0 36.0 8.0 38.0 80-81 30.254470995328028 34.0 30.0 35.5 7.0 37.0 82-83 29.891388795035205 34.0 30.0 35.0 4.0 37.0 84-85 29.495674639698944 34.0 30.0 35.0 2.0 36.5 86-87 29.075062720151614 34.0 29.0 35.0 2.0 36.0 88-89 28.68235107362142 34.0 29.0 35.0 2.0 36.0 90-91 28.14219027313364 33.5 28.5 35.0 2.0 35.0 92-93 27.657942643100977 33.0 27.0 35.0 2.0 35.0 94-95 27.16630112274897 33.0 26.0 35.0 2.0 35.0 96-97 26.58892426201877 33.0 25.0 35.0 2.0 35.0 98-99 25.677850465837906 32.0 23.5 34.0 2.0 35.0 100-101 23.034676185372028 29.5 9.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3578863770554932 1101 2 0.2623480713919548 1101 3 0.22948098123712413 1101 4 0.14364733765198423 1101 5 0.21245101814564293 1101 6 0.2192526025546826 1101 7 0.2505751726022325 1101 8 0.22141490756099813 1101 9 0.25437999461444605 1101 10-11 0.32545358827038484 1101 12-13 0.3289076057038969 1101 14-15 0.3092822984109347 1101 16-17 0.26905298767817953 1101 18-19 0.35478797026603104 1101 20-21 0.2625137529769219 1101 22-23 0.16284155595933925 1101 24-25 0.31161130791539904 1101 26-27 0.2529340373277975 1101 28-29 0.05576365853327303 1101 30-31 0.35018129129581155 1101 32-33 0.4454657033271445 1101 34-35 0.25109775283866753 1101 36-37 0.21285020702379143 1101 38-39 0.3536784112122433 1101 40-41 0.29635648120751057 1101 42-43 0.2783573454622399 1101 44-45 0.3578366977110292 1101 46-47 0.26359518145059013 1101 48-49 0.3478041676628152 1101 50-51 0.3899790802565448 1101 52-53 0.4453425840855658 1101 54-55 0.3874944807909415 1101 56-57 0.3841106666597085 1101 58-59 0.3351400220801537 1101 60-61 0.29599330558760784 1101 62-63 0.126050901949192 1101 64-65 0.2832830280086043 1101 66-67 0.05517435100343704 1101 68-69 0.20302789033491564 1101 70-71 0.2301600333220435 1101 72-73 0.181394712104602 1101 74-75 0.0971813548414957 1101 76-77 0.02880889702910494 1101 78-79 -0.03352761826848294 1101 80-81 0.3058930999164282 1101 82-83 0.28139136482375093 1101 84-85 0.13084843323274598 1101 86-87 -0.18614441150152672 1101 88-89 -0.14832572759331697 1101 90-91 -0.09302144992771844 1101 92-93 0.1435930080079828 1101 94-95 0.09428753962621528 1101 96-97 0.30256178453400295 1101 98-99 0.4762403249144782 1101 100-101 0.8472681250890695 1102 1 0.2388230776365603 1102 2 0.24145434996196968 1102 3 0.13521506902031888 1102 4 0.14546476735538505 1102 5 0.08926676780926357 1102 6 0.06161110960412941 1102 7 0.018661734225347004 1102 8 0.1261379847368005 1102 9 0.10040024170723427 1102 10-11 0.1677139063280535 1102 12-13 0.20422326651208067 1102 14-15 0.30151902124320173 1102 16-17 0.22965941483837327 1102 18-19 0.2018330416266494 1102 20-21 0.2330395083601431 1102 22-23 0.10792889562754482 1102 24-25 0.1229585206173951 1102 26-27 0.1286656013178984 1102 28-29 0.050987118758150984 1102 30-31 0.2633292079718075 1102 32-33 0.16581785459942466 1102 34-35 -0.009096355224478714 1102 36-37 0.10928882135684148 1102 38-39 0.05248647898728365 1102 40-41 -0.050246926361182886 1102 42-43 -0.060209871809462356 1102 44-45 0.06261413350737399 1102 46-47 0.13833253523112887 1102 48-49 0.04108751794588983 1102 50-51 -0.04279767456367267 1102 52-53 0.1245210670320418 1102 54-55 0.14955567306664364 1102 56-57 0.020708354626286507 1102 58-59 0.2710435176082626 1102 60-61 0.3323280859952078 1102 62-63 0.26689647717241627 1102 64-65 0.3533238865761348 1102 66-67 0.2951306998364416 1102 68-69 0.10761021751108757 1102 70-71 0.31413103508869966 1102 72-73 0.18649157946508765 1102 74-75 0.3046442408959713 1102 76-77 0.24473358345901275 1102 78-79 0.47251529816111315 1102 80-81 0.25345681822527766 1102 82-83 0.2939285683714026 1102 84-85 0.29194670137306744 1102 86-87 0.5128675011005619 1102 88-89 0.41304761906086185 1102 90-91 0.44372848697745226 1102 92-93 0.63086465326721 1102 94-95 0.34975625579208014 1102 96-97 0.4685530752077618 1102 98-99 0.3506931919583707 1102 100-101 0.8525434500087634 1103 1 0.21937541711596253 1103 2 0.23214524952246762 1103 3 0.17600403490303052 1103 4 0.14484411265828356 1103 5 0.0965843010884484 1103 6 0.13790339620547343 1103 7 0.05625670447424369 1103 8 0.11487043792129015 1103 9 0.08585517728010217 1103 10-11 0.16755802263671882 1103 12-13 0.14639941724332317 1103 14-15 0.22407506737037153 1103 16-17 0.32645094686184706 1103 18-19 -0.0015202937803238115 1103 20-21 0.0868555267234612 1103 22-23 0.057493861325930595 1103 24-25 0.19998054449892066 1103 26-27 0.16564819706576372 1103 28-29 0.10313075351941592 1103 30-31 0.20659978461905837 1103 32-33 0.2833656781318865 1103 34-35 0.28433975334257156 1103 36-37 0.21022045141783963 1103 38-39 0.17416216026742148 1103 40-41 0.18316676087493988 1103 42-43 0.3960822131871069 1103 44-45 0.4118490433900419 1103 46-47 0.5846930650484694 1103 48-49 0.5706247773881898 1103 50-51 0.5565355176463171 1103 52-53 0.44538982520423787 1103 54-55 0.5039595853512822 1103 56-57 0.41976196421576617 1103 58-59 0.3961598003618434 1103 60-61 0.30780358525503004 1103 62-63 0.3829481052749699 1103 64-65 0.2852671549927308 1103 66-67 0.36617838224556465 1103 68-69 0.3668285958022821 1103 70-71 0.3258075515886176 1103 72-73 0.40949058008142103 1103 74-75 0.4574736198956657 1103 76-77 0.6659813586063272 1103 78-79 0.677388229552129 1103 80-81 0.4536633191152184 1103 82-83 0.654706431591201 1103 84-85 0.3976977655582665 1103 86-87 0.504284537855991 1103 88-89 0.38019216104361675 1103 90-91 0.5868412358873485 1103 92-93 0.6924088306333331 1103 94-95 0.7498682904450611 1103 96-97 1.1864903559625724 1103 98-99 1.1102161374617197 1103 100-101 1.2782488507126502 1104 1 0.17764921929024524 1104 2 0.22041026166388633 1104 3 0.03948953281870615 1104 4 0.05911777372167393 1104 5 0.039920192056762005 1104 6 0.09657627763116494 1104 7 0.15869292528927303 1104 8 0.1041820225378558 1104 9 0.27213405356700804 1104 10-11 0.3144901100784452 1104 12-13 0.2900451642197268 1104 14-15 0.2571321320285591 1104 16-17 0.29557889622655864 1104 18-19 0.08248221387787424 1104 20-21 0.15405995819353535 1104 22-23 0.24354196250244087 1104 24-25 0.21913304127982514 1104 26-27 0.2816123225603846 1104 28-29 0.10616463777320462 1104 30-31 0.23780954596526271 1104 32-33 0.24398030427620654 1104 34-35 0.43298501525027433 1104 36-37 0.4214226721505554 1104 38-39 0.27446995840828237 1104 40-41 0.40106991176364204 1104 42-43 0.3343256177226479 1104 44-45 0.21236453913024889 1104 46-47 0.2418035107825176 1104 48-49 0.022359706450572503 1104 50-51 0.015210971406041551 1104 52-53 0.21945621494077017 1104 54-55 0.23619284166159105 1104 56-57 0.053273450054696525 1104 58-59 0.18846477334498246 1104 60-61 0.013714514881847606 1104 62-63 0.1380185431303147 1104 64-65 0.2121254090981779 1104 66-67 0.14910646272581118 1104 68-69 -0.0370432813842001 1104 70-71 0.2595170336528341 1104 72-73 0.234015139256865 1104 74-75 0.11647767095428918 1104 76-77 0.2951738956038419 1104 78-79 0.3629791902233457 1104 80-81 0.45244182792582777 1104 82-83 0.71416960752709 1104 84-85 0.5581900955931367 1104 86-87 0.4562131269234264 1104 88-89 0.43097113728407166 1104 90-91 0.5141198828690463 1104 92-93 0.6845418982078115 1104 94-95 0.5110807270186548 1104 96-97 0.6517098275022697 1104 98-99 0.7050191407719488 1104 100-101 1.03207832113522 1105 1 0.18910744546161595 1105 2 0.16092870904007128 1105 3 0.06293613180782387 1105 4 0.11038280424264713 1105 5 0.10197963906725249 1105 6 0.14442464067198557 1105 7 0.12054714697617896 1105 8 0.022311911469877543 1105 9 0.031216944923741607 1105 10-11 0.11158887129243311 1105 12-13 0.1985715893267539 1105 14-15 0.043096311836876566 1105 16-17 0.1483918648450313 1105 18-19 0.11550712100834915 1105 20-21 0.29865708053350914 1105 22-23 0.2852793717073183 1105 24-25 0.3071178607633911 1105 26-27 0.023061079681887975 1105 28-29 0.03658702047450646 1105 30-31 0.14709739982500736 1105 32-33 0.09059408643699385 1105 34-35 0.08776305576520116 1105 36-37 0.02212425290368003 1105 38-39 -0.115590317613389 1105 40-41 -0.12716527810921008 1105 42-43 -0.1452689928035369 1105 44-45 -0.2816938385759471 1105 46-47 -0.2678187259407281 1105 48-49 -0.27479875257984077 1105 50-51 -0.4229931530747919 1105 52-53 -0.47795507748315913 1105 54-55 -0.3746198220850161 1105 56-57 -0.35529314905456744 1105 58-59 -0.26603685501621044 1105 60-61 -0.20767615138333184 1105 62-63 -0.32531873751775464 1105 64-65 -0.2619743741123912 1105 66-67 -0.3379601367589089 1105 68-69 -0.5826201237376587 1105 70-71 -0.47310658400480676 1105 72-73 -0.32612271388951086 1105 74-75 -0.38834959209982145 1105 76-77 -0.4070263631617905 1105 78-79 -0.368854463876243 1105 80-81 -0.30765780359972084 1105 82-83 -0.2050735142707225 1105 84-85 -0.15794078004241996 1105 86-87 -0.062337875661977904 1105 88-89 -0.24058236510046171 1105 90-91 -0.047754087906806575 1105 92-93 0.005543815296178423 1105 94-95 0.06582099646231043 1105 96-97 0.22768900310170537 1105 98-99 0.22444984136899748 1105 100-101 0.2307166101882956 1106 1 0.045320239489356595 1106 2 0.09021403259125549 1106 3 0.14350214525828875 1106 4 0.026627937299252835 1106 5 0.024664422025715282 1106 6 0.03248011578219234 1106 7 0.09206547242586538 1106 8 0.04663536611479202 1106 9 0.15843643200421553 1106 10-11 0.0668777505278797 1106 12-13 -0.08912590399628328 1106 14-15 -0.005885250089946226 1106 16-17 0.1152170088422011 1106 18-19 0.18748197379336773 1106 20-21 0.023382359678862485 1106 22-23 -0.0075288144110317035 1106 24-25 0.16617479997889717 1106 26-27 0.10350722956765424 1106 28-29 0.3168968154997671 1106 30-31 0.22784883979669246 1106 32-33 0.05005124124602389 1106 34-35 0.0983420209162702 1106 36-37 -0.010849087508844946 1106 38-39 0.15936624190007365 1106 40-41 0.15965013200116118 1106 42-43 -0.21483048522559045 1106 44-45 -0.19873296719197242 1106 46-47 -0.023441855586440852 1106 48-49 0.08605372674571043 1106 50-51 0.0722762583870562 1106 52-53 -0.1845515958086068 1106 54-55 0.01505180001493045 1106 56-57 -0.10383025044787786 1106 58-59 -0.18362100084753763 1106 60-61 0.06391658601088324 1106 62-63 0.015997991896277597 1106 64-65 0.04082271054828368 1106 66-67 0.11527135276710254 1106 68-69 0.2149106063843007 1106 70-71 0.26781435430969935 1106 72-73 0.21953404191059533 1106 74-75 -0.07415903316555728 1106 76-77 0.033460572514112386 1106 78-79 0.07158702017949281 1106 80-81 -0.14761219108886436 1106 82-83 -0.1957470831480279 1106 84-85 -0.0672776690600223 1106 86-87 0.09500638214926482 1106 88-89 -0.22323754417180197 1106 90-91 -0.1404641307037302 1106 92-93 -0.03969623361459185 1106 94-95 0.1336834831888254 1106 96-97 0.2175750120472273 1106 98-99 0.46552603920019564 1106 100-101 0.6169109822319285 1107 1 0.2875900912676457 1107 2 0.2506171367878025 1107 3 0.14045863501517175 1107 4 0.006636396602644368 1107 5 0.10720975285803291 1107 6 0.11627503284424279 1107 7 0.09549057081124346 1107 8 0.0894725429142369 1107 9 0.12775490638723852 1107 10-11 0.16902521825380035 1107 12-13 0.15732210806742586 1107 14-15 0.3272605073855033 1107 16-17 0.33747876911289865 1107 18-19 0.3330201509973847 1107 20-21 0.3509638264485133 1107 22-23 0.27034826823268787 1107 24-25 0.25812329017713154 1107 26-27 0.29619820753668336 1107 28-29 0.27588068765394524 1107 30-31 0.20082642264269168 1107 32-33 0.21598783325013926 1107 34-35 0.19191065161250975 1107 36-37 -0.04065479937761296 1107 38-39 0.06296604329033784 1107 40-41 0.15234340869394458 1107 42-43 0.08821348476227087 1107 44-45 0.079266407555167 1107 46-47 0.14013532636213455 1107 48-49 0.24953482204160338 1107 50-51 0.24080810950331966 1107 52-53 0.22490811216152196 1107 54-55 0.31550326935914796 1107 56-57 0.23549401671899517 1107 58-59 0.1847067235998523 1107 60-61 0.34835560546728317 1107 62-63 0.36241749978550075 1107 64-65 0.34844987594605215 1107 66-67 0.3890852113128389 1107 68-69 0.18195012604298455 1107 70-71 0.2111112758588618 1107 72-73 0.4376231588328814 1107 74-75 0.49293795458684286 1107 76-77 0.4602062233287789 1107 78-79 0.6107315271492197 1107 80-81 0.6953744027542363 1107 82-83 0.6047149603254347 1107 84-85 0.573417556950492 1107 86-87 0.6192666528469779 1107 88-89 0.7969572037041779 1107 90-91 0.9436893872630918 1107 92-93 0.9862600354648166 1107 94-95 1.3637632029284248 1107 96-97 1.3213233109916551 1107 98-99 1.5621377203947908 1107 100-101 1.3968417446726207 1108 1 0.160385756269676 1108 2 0.12734466502874398 1108 3 0.02089217066275495 1108 4 0.03313950301659219 1108 5 -0.0823585757012566 1108 6 -0.034500764014573804 1108 7 0.05664964143241491 1108 8 0.03583178274803345 1108 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-0.2343960237515752 2316 78-79 -0.4425237848404038 2316 80-81 -0.5896576456343148 2316 82-83 -0.8420165964629938 2316 84-85 -0.6071498759776865 2316 86-87 -0.5601550809667408 2316 88-89 -0.5039767544564668 2316 90-91 -0.4271338582825486 2316 92-93 -0.3906068549797581 2316 94-95 -0.440891815250108 2316 96-97 -0.2233532095628128 2316 98-99 -0.4576986317517502 2316 100-101 0.06878196478472631 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 8.0 5 21.0 6 49.0 7 126.0 8 273.0 9 561.0 10 853.0 11 1676.0 12 2622.0 13 3686.0 14 4904.0 15 7141.0 16 10072.0 17 14434.0 18 12692.0 19 7349.0 20 5719.0 21 5530.0 22 5826.0 23 6171.0 24 7010.0 25 8305.0 26 10100.0 27 12313.0 28 15602.0 29 19200.0 30 24462.0 31 30353.0 32 38817.0 33 51345.0 34 71368.0 35 102279.0 36 153479.0 37 221485.0 38 160844.0 39 13291.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.393617217566785 19.93495439135548 15.502083537673139 21.1693448534046 2 15.757030288202008 20.91289860306882 40.13808142357268 23.19198968515649 3 19.3614001157313 25.53418927893186 30.303930592287948 24.800480013048897 4 12.722033381587213 17.43770929027961 36.79013640216356 33.050120925969615 5 13.040214352238063 38.56873848580227 33.63733566927291 14.753711492686755 6 31.174730963560176 36.95479100402729 17.03046296404174 14.840015068370791 7 28.257078833211004 34.29102927069862 19.831121622723725 17.620770273366652 8 27.21870109090344 34.86754979747022 18.959156171235723 18.95459294039061 9 27.3451122943148 16.485496693113987 20.30191111991394 35.86747989265728 10-11 25.138062005569083 27.472057492825048 26.163575320494147 21.22630518111172 12-13 26.215229146954748 25.117931684336796 27.43195110004034 21.234888068668113 14-15 23.031208615379835 25.866868225543993 26.63693770316086 24.464985455915304 16-17 21.24984951047213 29.47075260298338 27.430745690174103 21.848652196370384 18-19 21.247373715013612 26.886507594381204 31.220703087074213 20.64541560353097 20-21 21.981811156031426 25.810507470105982 31.715570908723734 20.492110465138857 22-23 21.921429178246782 25.714771168853233 31.57357670788985 20.790222945010132 24-25 21.515390709650358 26.048572194195568 31.76688298322673 20.66915411292734 26-27 21.036333167632392 26.53810603824585 31.515542230438225 20.910018563683533 28-29 20.927861726601872 26.5519178181278 31.729742771439074 20.790477683831256 30-31 21.040960336785854 26.247461096029795 31.750523315196915 20.961055251987432 32-33 20.940617803202414 26.306054921881373 31.506536100010486 21.246791174905724 34-35 21.57689723604137 26.400086604296042 31.28051053815055 20.74250562151204 36-37 21.592140368864396 26.081631345318122 31.189294466257333 21.136933819560145 38-39 21.191595499683487 26.463583476055657 31.176527128892822 21.16829389536803 40-41 21.447531814126084 26.406088738765416 31.170367594301563 20.976011852806934 42-43 21.257497822390583 26.491431823103344 31.022850090163228 21.22822026434285 44-45 21.449952037531116 26.663637458105654 30.777535699999614 21.108874804363616 46-47 21.778164843316127 27.251517516981043 29.759498316459087 21.210819323243737 48-49 21.76428097074484 27.301955781322214 29.48793753616603 21.445825711766922 50-51 22.029191084806186 27.479727604245554 29.156035309697742 21.335046001250518 52-53 22.141038785520383 27.49031041620549 29.101033814511464 21.267616983762665 54-55 21.808796743989156 27.666431708823154 29.12006345803575 21.40470808915194 56-57 21.973558504503035 28.108142745628033 28.813695906296484 21.104602843572444 58-59 21.97147106911644 27.44045469197221 28.86500798079948 21.72306625811187 60-61 21.731658724703195 27.659198502483562 29.371963509687642 21.2371792631256 62-63 21.64602532884389 27.594730730544132 29.516190731398524 21.243053209213457 64-65 21.62505388495998 27.5456517264547 29.234289864190483 21.595004524394838 66-67 21.703551164497675 27.809736575363214 28.868454676437803 21.618257583701308 68-69 21.840701417466406 27.548383620323474 29.04187154693115 21.569043415278966 70-71 21.71491069660146 27.80332863417646 28.93379625853907 21.54796441068301 72-73 21.855011592548145 27.62541117622615 28.75728660584948 21.762290625376224 74-75 21.75826138963001 28.281933877814154 28.296254655466363 21.663550077089475 76-77 21.859332098348304 27.85260181830186 28.715829168171563 21.572236915178276 78-79 21.894818499920383 27.943283895096176 28.485822915574406 21.676074689409035 80-81 21.785068810156396 28.364474162634597 28.35840603356501 21.492050993644 82-83 21.90086897422205 28.307879023253555 28.141317539686394 21.649934462838 84-85 21.75255214611349 28.201888119662144 28.200431761615548 21.845127972608818 86-87 21.77617449794752 28.133725965366047 28.15712465969949 21.932974876986947 88-89 21.779766828612818 28.094453053092707 28.201446252907846 21.924333865386632 90-91 21.778186468475415 28.141121933646456 28.246380287557276 21.834311310320853 92-93 21.79242580914515 27.82687425056678 28.439139954075216 21.941559986212855 94-95 21.616267238332693 27.778813404636242 28.4201900634192 22.184729293611866 96-97 21.89074071916518 27.738229777120154 28.119016827641918 22.252012676072745 98-99 21.484485935030982 27.815700238603235 28.084741772072014 22.615072054293766 100-101 21.502024493973614 27.616341033645103 28.55641125365193 22.32522321872935 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 480.0 1 343.5 2 276.0 3 383.0 4 328.5 5 294.5 6 529.0 7 1179.5 8 1954.5 9 2359.5 10 2213.0 11 1934.0 12 1966.5 13 2121.5 14 2264.0 15 2407.5 16 2514.0 17 2586.0 18 2727.5 19 2770.5 20 2898.0 21 3279.0 22 3714.5 23 4444.0 24 5303.0 25 6255.0 26 7798.0 27 9410.0 28 10318.5 29 11510.0 30 13360.5 31 15343.5 32 17977.5 33 20298.0 34 22244.0 35 24088.0 36 27055.5 37 29959.5 38 30953.0 39 31000.0 40 31048.5 41 32493.5 42 33306.0 43 34024.0 44 35858.5 45 37271.5 46 37028.0 47 36759.0 48 37480.0 49 36841.0 50 35895.0 51 34244.0 52 31820.5 53 31087.0 54 30795.0 55 28387.5 56 24951.0 57 22342.5 58 20742.5 59 18515.0 60 15114.0 61 12315.5 62 10416.5 63 8088.0 64 6010.0 65 4725.5 66 3387.0 67 2630.5 68 2062.0 69 1448.0 70 989.0 71 707.5 72 583.5 73 519.0 74 412.5 75 324.0 76 240.0 77 151.0 78 111.5 79 77.0 80 55.0 81 37.5 82 23.5 83 20.5 84 12.5 85 6.0 86 5.5 87 1.5 88 1.0 89 2.0 90 1.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022621974189589618 2 0.0 3 0.0 4 2.91270053943214E-4 5 0.025728854764983905 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0031554255843848186 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.223551708201776E-4 24-25 0.0 26-27 3.883600719242853E-4 28-29 0.0012136252247633916 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 4.854500899053566E-4 42-43 0.01679657311072534 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.854500899053566E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.854500899053566E-5 82-83 0.002135980395583569 84-85 5.339950988958922E-4 86-87 0.0 88-89 0.0 90-91 0.012136252247633917 92-93 0.0026214304854889257 94-95 0.0 96-97 0.0 98-99 0.001893255350630891 100-101 0.020243268749053373 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1029972.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.06828128739428 #Duplication Level Percentage of deduplicated Percentage of total 1 77.51445562162259 40.36044479145885 2 12.493429265906977 13.010227785228166 3 3.922454505915037 6.127063936529739 4 1.777008621891379 3.701031390990608 5 0.967597322410147 2.5190564778090536 6 0.6124282425114538 1.9132851599658538 7 0.42742860533486027 1.5578830996997899 8 0.30531481618666717 1.2717774184333175 9 0.24362620576498226 1.1416678029676524 >10 1.5418420729295355 15.307360185296037 >50 0.13198500064544047 4.712976196549171 >100 0.059439073812080835 5.560194357969977 >500 0.002056068484809979 0.7193186752800016 >1k 5.607459504027216E-4 0.7262286503774011 >5k 3.7383063360181437E-4 1.3714840714444299 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7616 0.7394375769438393 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6476 0.6287549564454179 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4541 0.4408857716520449 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1761 0.17097552166466662 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1160 0.11262442085804275 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 9.709001798107132E-5 0.0 0.0 0.0 0.0 20-21 1.9418003596214265E-4 0.0 0.0 0.0 0.0 22-23 1.9418003596214265E-4 0.0 0.0 0.0 0.0 24-25 1.9418003596214265E-4 0.0 0.0 9.709001798107132E-5 0.0 26-27 1.9418003596214265E-4 0.0 0.0 1.9418003596214265E-4 0.0 28-29 2.91270053943214E-4 0.0 0.0 5.339950988958923E-4 0.0 30-31 6.796301258674993E-4 0.0 0.0 0.0011165352067823203 0.0 32-33 6.796301258674993E-4 0.0 0.0 0.0016019852966876768 0.0 34-35 7.28175134858035E-4 0.0 0.0 0.002621430485488926 0.0 36-37 8.73810161829642E-4 0.0 0.0 0.003640875674290175 0.0 38-39 9.709001798107133E-4 0.0 0.0 0.004805955890063031 0.0 40-41 9.709001798107133E-4 0.0 0.0 0.00771865642949517 0.0 42-43 0.0010679901977917847 0.0 0.0 0.011893527202681237 0.0 44-45 0.001165080215772856 0.0 0.0 0.020680173829968194 0.0 46-47 0.0013592602517349985 0.0 0.0 0.035583491590062646 0.0 48-49 0.00145635026971607 0.0 0.0 0.049321729134384235 0.0 50-51 0.00145635026971607 0.0 0.0 0.0679144675777594 0.0 52-53 0.00145635026971607 0.0 0.0 0.09233260709999884 0.0 54-55 0.00145635026971607 0.0 0.0 0.11849836694589755 0.0 56-57 0.00145635026971607 0.0 0.0 0.14942153767286875 0.0 58-59 0.00145635026971607 0.0 0.0 0.18869444994621215 0.0 60-61 0.0015048952787066056 0.0 0.0 0.24083178960204743 0.0 62-63 0.0015534402876971412 0.0 0.0 0.30321212615488574 0.0 64-65 0.0015534402876971412 0.0 0.0 0.36195158703343394 0.0 66-67 0.0016505303056782127 0.0 0.0 0.438992516301414 0.0 68-69 0.001747620323659284 0.0 0.0 0.5339465538869017 0.0 70-71 0.0017961653326498195 0.0 0.0 0.6206479399439985 0.0 72-73 0.0018447103416403552 0.0 0.0 0.7354083411976248 0.0 74-75 0.001893255350630891 0.0 0.0 0.8386150303115036 0.0 76-77 0.0019418003596214266 0.0 0.0 0.9542492417269596 0.0 78-79 0.0019903453686119623 0.0 0.0 1.0907092619993555 0.0 80-81 0.0022330704135646406 0.0 0.0 1.2327519583056628 0.0 82-83 0.0022330704135646406 0.0 0.0 1.395911733522853 0.0 84-85 0.0022816154225551765 0.0 0.0 1.5924704749255318 0.0 86-87 0.002330160431545712 0.0 0.0 1.8232049026575479 0.0 88-89 0.002330160431545712 0.0 0.0 2.103697964604863 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3750 0.0 52.200405 1 GTATCAA 6535 0.0 40.56921 1 ATCAACG 8700 0.0 30.523703 3 TCAACGC 8840 0.0 30.040295 4 TATCAAC 8955 0.0 29.654518 2 CAACGCA 8975 0.0 29.53407 5 AACGCAG 9245 0.0 28.718729 6 ATATGCG 35 0.008394237 27.142096 88-89 TGGTATC 1830 0.0 25.959335 2 ACGCAGA 10565 0.0 25.04797 7 CGCAGAG 10605 0.0 24.953493 8 TAAGGTG 530 0.0 24.199781 5 ATGGGCG 140 1.778298E-5 23.751638 5 GTACATG 6955 0.0 23.568388 1 GCAGAGT 11170 0.0 23.351028 9 GGACCGA 285 3.6379788E-12 23.331545 6 TACATGG 7025 0.0 23.262522 2 GAGTACT 6615 0.0 22.947102 12-13 GTATAGG 315 0.0 22.625004 1 GTAAGGT 485 0.0 22.528423 4 >>END_MODULE