FastQCFastQC Report
Thu 26 May 2016
SRR937557_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937557_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976515
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67000.686113372554441No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52550.5381381750408339No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35810.36671223688320204No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14170.14510785804621537No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13420.13742748447284475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28250.055.7088361
GTATCAA56000.046.10271
ATCAACG72250.035.130483
TCAACGC73350.034.6018684
CAACGCA73800.034.2620745
TATCAAC74600.034.220242
AACGCAG76000.033.3191266
ACGCAGA86550.029.1479137
CGCAGAG86250.029.1391288
TGGTATC12450.029.0196292
GTACATG63300.027.190641
GCAGAGT91900.026.93279
TACATGG64650.026.3981512
ACATGGG63000.025.953663
GTGGTAT15400.024.3905121
GAGTACT55000.023.92257312-13
ATGGGTA3400.023.7644845
GTACTTT57000.023.04033314-15
CATGGGA33500.022.5585254
AGTACTT57850.022.29242112-13
Produced by FastQC (version 0.11.5)