##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937557_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 976515 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.790532659508557 33.0 31.0 34.0 30.0 34.0 2 31.919085728329826 33.0 31.0 34.0 30.0 34.0 3 31.925360081514366 34.0 31.0 34.0 30.0 34.0 4 35.53461544369518 37.0 35.0 37.0 33.0 37.0 5 35.53266770095698 37.0 35.0 37.0 33.0 37.0 6 35.589173745410974 37.0 35.0 37.0 33.0 37.0 7 35.5260963733276 37.0 35.0 37.0 33.0 37.0 8 35.512076107381866 37.0 35.0 37.0 33.0 37.0 9 37.13641060301173 39.0 37.0 39.0 34.0 39.0 10-11 37.027833673829896 39.0 37.0 39.0 33.0 39.0 12-13 36.8979795497253 39.0 37.0 39.0 33.0 39.0 14-15 38.04323640701884 40.0 38.0 41.0 33.0 41.0 16-17 37.97996292939689 40.0 38.0 41.0 33.0 41.0 18-19 37.95663865890437 40.0 38.0 41.0 33.0 41.0 20-21 37.89062840816577 40.0 38.0 41.0 33.0 41.0 22-23 37.703477161129115 40.0 37.5 41.0 32.5 41.0 24-25 37.533776234876065 40.0 37.0 41.0 32.0 41.0 26-27 37.467090623287916 40.0 37.0 41.0 32.0 41.0 28-29 37.49255106168364 40.0 37.0 41.0 32.0 41.0 30-31 37.264693834708126 39.5 37.0 41.0 32.0 41.0 32-33 36.98580154938736 39.0 36.0 41.0 31.0 41.0 34-35 36.97907303011218 39.5 36.0 41.0 31.0 41.0 36-37 36.87012283477468 40.0 36.0 41.0 31.0 41.0 38-39 36.723148133925235 39.5 36.0 41.0 30.5 41.0 40-41 36.46268311290662 39.0 35.0 41.0 30.0 41.0 42-43 36.161815230692824 39.0 35.0 41.0 30.0 41.0 44-45 35.85186761084059 39.0 35.0 41.0 28.5 41.0 46-47 35.40518630026165 39.0 35.0 41.0 26.5 41.0 48-49 34.96668356348853 39.0 35.0 40.0 24.0 41.0 50-51 34.01212014152368 38.0 34.0 39.5 23.0 40.5 52-53 34.08629411734587 38.0 34.0 39.5 23.0 40.5 54-55 34.41698181799563 38.0 34.0 40.0 23.0 41.0 56-57 34.21282980804186 38.0 34.0 40.0 22.0 41.0 58-59 33.97314634183807 38.0 34.0 40.0 20.5 41.0 60-61 33.65290804544733 38.0 33.5 40.0 19.5 41.0 62-63 33.29499393250488 37.0 33.0 40.0 18.0 41.0 64-65 32.92931752200427 37.0 33.0 40.0 14.0 41.0 66-67 32.519085216304916 36.0 32.0 39.0 10.0 41.0 68-69 32.04233677926094 35.5 31.5 39.0 7.5 40.0 70-71 31.610555393414337 35.0 31.0 38.5 6.5 40.0 72-73 31.16123971469972 35.0 31.0 37.5 2.0 40.0 74-75 30.71978669042462 35.0 30.5 37.0 2.0 39.0 76-77 30.234664086061144 35.0 30.0 36.5 2.0 39.0 78-79 29.721282315171813 34.0 29.5 36.0 2.0 38.5 80-81 29.216271639452543 34.0 29.0 35.5 2.0 37.5 82-83 28.713756061094813 34.0 29.0 35.0 2.0 37.0 84-85 28.2557938178113 34.0 27.5 35.0 2.0 36.5 86-87 27.796693855189115 34.0 26.5 35.0 2.0 36.0 88-89 27.32931957010389 33.0 26.0 35.0 2.0 36.0 90-91 26.84286928516203 33.0 25.0 35.0 2.0 35.0 92-93 26.300685089322744 33.0 24.5 35.0 2.0 35.0 94-95 25.686391914102703 32.0 21.5 35.0 2.0 35.0 96-97 24.984965924742582 32.0 19.0 34.0 2.0 35.0 98-99 24.094233575521116 31.5 2.0 34.0 2.0 35.0 100-101 21.7967235526336 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.012172099901270883 1101 2 0.10447981344731616 1101 3 0.004788059494170938 1101 4 -0.046339459310956954 1101 5 -0.02938569614096309 1101 6 0.043065959190236924 1101 7 0.037844295501848535 1101 8 0.012796585570555408 1101 9 0.2722837933200495 1101 10-11 0.045851557620693484 1101 12-13 0.1770244601019968 1101 14-15 0.23116566075800193 1101 16-17 0.31247697397916596 1101 18-19 0.2846042964328461 1101 20-21 0.3505226109805122 1101 22-23 0.21018473493206358 1101 24-25 0.20905886355857461 1101 26-27 0.23322887479943688 1101 28-29 0.12658972587340145 1101 30-31 0.34148878650291437 1101 32-33 0.23830196985949925 1101 34-35 0.3688269640564954 1101 36-37 0.246545480873273 1101 38-39 0.5548069694235593 1101 40-41 0.344006821543104 1101 42-43 0.25276088989142664 1101 44-45 0.39234593865574396 1101 46-47 0.47403414755259377 1101 48-49 0.30511576542462393 1101 50-51 0.1060551254862574 1101 52-53 0.24103147161458338 1101 54-55 0.3083589115815073 1101 56-57 0.24346791445289284 1101 58-59 0.2637791190854486 1101 60-61 0.2404081378607259 1101 62-63 0.4147486296269136 1101 64-65 0.3594346250069762 1101 66-67 0.24341607528118914 1101 68-69 0.233663877673024 1101 70-71 0.2927559903092849 1101 72-73 0.290975324884613 1101 74-75 0.38232104522056076 1101 76-77 0.4456750292475533 1101 78-79 0.35662999370016024 1101 80-81 0.39298141430179 1101 82-83 0.5112501430175236 1101 84-85 0.7681214836003427 1101 86-87 0.8854701003748993 1101 88-89 0.8704915658054873 1101 90-91 1.035080204609212 1101 92-93 0.9376635432568996 1101 94-95 1.3057496710918635 1101 96-97 1.3881979691007587 1101 98-99 1.5045955056570222 1101 100-101 1.6871499383669892 1102 1 0.33321572821653334 1102 2 0.2597388448461935 1102 3 0.32560060404156843 1102 4 0.3235513761168889 1102 5 0.36627473076379147 1102 6 0.31781630619101975 1102 7 0.2698861606779772 1102 8 0.3763371190139324 1102 9 0.43317286560875345 1102 10-11 0.36637899881813496 1102 12-13 0.39703758986153304 1102 14-15 0.6365563648882429 1102 16-17 0.5390324753484137 1102 18-19 0.6117356315418263 1102 20-21 0.7375417804294457 1102 22-23 0.6501937381957461 1102 24-25 0.7966111012447357 1102 26-27 0.6316097878804285 1102 28-29 0.6588356650263449 1102 30-31 0.6607651491843853 1102 32-33 0.5394712687303453 1102 34-35 0.6565738973198023 1102 36-37 0.6840103118745162 1102 38-39 0.7861281983690489 1102 40-41 0.5410916397897196 1102 42-43 0.6862076393381074 1102 44-45 0.8806341931479906 1102 46-47 0.866841665277768 1102 48-49 0.7381198544068752 1102 50-51 0.5398972682630117 1102 52-53 0.5318530194256681 1102 54-55 0.5260008067685149 1102 56-57 0.596487384010608 1102 58-59 0.6486615139535914 1102 60-61 0.6166972926793477 1102 62-63 0.6080603301183345 1102 64-65 0.6642536218933515 1102 66-67 0.7114957541647797 1102 68-69 0.6291288587286559 1102 70-71 0.6257195646049674 1102 72-73 0.686047163426089 1102 74-75 0.7753787786489283 1102 76-77 0.8488460537439302 1102 78-79 1.0574391620236767 1102 80-81 1.2264307897003697 1102 82-83 1.2323432892830404 1102 84-85 1.4346274670193324 1102 86-87 1.2311087693924172 1102 88-89 1.2279530205828415 1102 90-91 1.2261414643158588 1102 92-93 1.4164379739880424 1102 94-95 1.5165738448548503 1102 96-97 1.525597526440297 1102 98-99 1.4766013577784172 1102 100-101 2.1959483778012867 1103 1 0.15227440169155315 1103 2 0.05406208113699762 1103 3 0.06028678072178906 1103 4 -0.022451991280263428 1103 5 0.006147125683419574 1103 6 -0.18303804762988563 1103 7 -0.039683411233021104 1103 8 -0.1968368330142667 1103 9 -0.13694041810364865 1103 10-11 -0.10444682260095561 1103 12-13 -0.011202308866458566 1103 14-15 0.1352577323692543 1103 16-17 -0.02698594162185941 1103 18-19 -0.06001633442819099 1103 20-21 0.0773171548850371 1103 22-23 -0.016601283823270307 1103 24-25 -0.012322210610221873 1103 26-27 -0.09246592485955318 1103 28-29 -0.2893352528137214 1103 30-31 0.14161666374076276 1103 32-33 0.04352788631303639 1103 34-35 -0.0699368893109451 1103 36-37 -0.021144203696202624 1103 38-39 -0.08937887882878925 1103 40-41 -0.19869565075016027 1103 42-43 -0.09147610925605676 1103 44-45 -0.21873675529139547 1103 46-47 -0.08416704596829305 1103 48-49 -0.04696437097776851 1103 50-51 0.020181297694271905 1103 52-53 0.05924971543385027 1103 54-55 -0.13663682584390102 1103 56-57 -0.10507428503559879 1103 58-59 -0.07842889455478996 1103 60-61 0.10047633905425357 1103 62-63 0.4086019970608419 1103 64-65 0.2105343692525068 1103 66-67 -0.11984053170942843 1103 68-69 0.04141323828683596 1103 70-71 0.061801173599992154 1103 72-73 0.07765051670047285 1103 74-75 -0.02572669561490315 1103 76-77 0.0568173992390264 1103 78-79 0.29425147707612 1103 80-81 0.2460419428610372 1103 82-83 0.29600717626560424 1103 84-85 0.38188959954237234 1103 86-87 0.28053403899382445 1103 88-89 0.10506104577138942 1103 90-91 0.17248855925542017 1103 92-93 0.3259073625237363 1103 94-95 0.3510693641864897 1103 96-97 0.7315398275832834 1103 98-99 0.8440157955120924 1103 100-101 1.4224952069091898 1104 1 0.1713659955685536 1104 2 0.13140762669099715 1104 3 0.2099064820378942 1104 4 0.11397557865225139 1104 5 0.13439173747063649 1104 6 0.23540456833917744 1104 7 0.16072647734750234 1104 8 0.2425816495980584 1104 9 0.31277712614972586 1104 10-11 0.15483807218270584 1104 12-13 0.19652395014846036 1104 14-15 0.48421593839937316 1104 16-17 0.5072909353737955 1104 18-19 0.4199595640640226 1104 20-21 0.40072583690881913 1104 22-23 0.34537832840465654 1104 24-25 0.31268595531243903 1104 26-27 0.4797993428234619 1104 28-29 0.4822585204943408 1104 30-31 0.5307967606652682 1104 32-33 0.5560985928338553 1104 34-35 0.3633243765144698 1104 36-37 0.48884895988963706 1104 38-39 0.5762956560098829 1104 40-41 0.47538256286445346 1104 42-43 0.5595342061004018 1104 44-45 0.5255080283320197 1104 46-47 0.7895340908910882 1104 48-49 0.8018675502620027 1104 50-51 0.7767108879612366 1104 52-53 0.7099533920618342 1104 54-55 0.7152270413741277 1104 56-57 0.7361457371401556 1104 58-59 0.6215432561307423 1104 60-61 0.682740325372535 1104 62-63 0.6839395033473039 1104 64-65 0.5099593105356277 1104 66-67 0.48344931669696933 1104 68-69 0.7312837167543549 1104 70-71 0.495324267421811 1104 72-73 0.7063521238873705 1104 74-75 0.7463092596276901 1104 76-77 0.7118469780255516 1104 78-79 0.8144674129575193 1104 80-81 0.8564400325165771 1104 82-83 0.8803250495260393 1104 84-85 0.9205957026159446 1104 86-87 1.1924587302997267 1104 88-89 1.2061222083469438 1104 90-91 1.2515528371026932 1104 92-93 1.337161522329918 1104 94-95 1.2510962506257286 1104 96-97 1.4609604063009272 1104 98-99 1.6796659547938795 1104 100-101 2.0460839466017937 1105 1 0.23357402012607764 1105 2 0.16033181563293297 1105 3 0.24510497464520853 1105 4 0.2347970639893333 1105 5 0.16066660266474742 1105 6 0.39607977544189055 1105 7 0.2247867668155621 1105 8 0.2566261070755118 1105 9 0.17095368638069885 1105 10-11 0.35512874129098293 1105 12-13 0.3642050080685877 1105 14-15 0.22991673405439172 1105 16-17 0.4724309198069676 1105 18-19 0.43758376720015946 1105 20-21 0.5303063905449577 1105 22-23 0.3184596549226981 1105 24-25 0.3626565530441752 1105 26-27 0.314114051600626 1105 28-29 0.3694154225403281 1105 30-31 0.46387189476924817 1105 32-33 0.39878592895715315 1105 34-35 0.49270956494635243 1105 36-37 0.5503273870476662 1105 38-39 0.5719477078514714 1105 40-41 0.6490470213713735 1105 42-43 0.7474115807040178 1105 44-45 0.3576023589195785 1105 46-47 0.36581542926362687 1105 48-49 0.46318851540510764 1105 50-51 0.4688987754745497 1105 52-53 0.34403982381191156 1105 54-55 0.496879714016913 1105 56-57 0.44221274311576053 1105 58-59 0.4955432280543022 1105 60-61 0.33243998549167486 1105 62-63 0.5994945511006478 1105 64-65 0.6438683148048128 1105 66-67 0.4857703021175013 1105 68-69 0.4384433640519205 1105 70-71 0.5597047062565217 1105 72-73 0.5835668477946143 1105 74-75 0.5971184849208164 1105 76-77 0.6955844820615411 1105 78-79 0.7615211029031883 1105 80-81 0.9568312308656779 1105 82-83 1.0642233691513105 1105 84-85 1.0567913766609927 1105 86-87 1.1432072013037882 1105 88-89 1.0740900358702739 1105 90-91 1.090950940695386 1105 92-93 1.3868572049538805 1105 94-95 1.2156411705844867 1105 96-97 1.438930152380756 1105 98-99 1.656553198264934 1105 100-101 1.61911621734599 1106 1 0.13637955592168893 1106 2 0.274493485856393 1106 3 0.22711966098901826 1106 4 0.12826085059698045 1106 5 0.11680517068977281 1106 6 0.10090571855583619 1106 7 0.11360037972389136 1106 8 -0.06643958250892723 1106 9 0.17780380061199708 1106 10-11 0.09519983945462229 1106 12-13 -0.01428262220590426 1106 14-15 -0.07662305494704214 1106 16-17 0.04491886624058594 1106 18-19 0.2168821197853248 1106 20-21 0.21881720582145903 1106 22-23 0.23467748855525628 1106 24-25 0.08551862110118691 1106 26-27 0.17000947820842072 1106 28-29 0.20315430521895195 1106 30-31 0.29523958406011275 1106 32-33 0.24705778063336226 1106 34-35 0.23660656482214648 1106 36-37 0.43135087691739926 1106 38-39 0.5293753770436425 1106 40-41 0.7210070949912861 1106 42-43 0.5522021441070919 1106 44-45 0.30708935311204044 1106 46-47 0.7229594961990244 1106 48-49 0.778684264194105 1106 50-51 0.6073576503244666 1106 52-53 0.5361996422462525 1106 54-55 0.46787399680619046 1106 56-57 0.3177382635550714 1106 58-59 0.39044760873999707 1106 60-61 0.37209349015314075 1106 62-63 0.4636119966855219 1106 64-65 0.6259262848374405 1106 66-67 0.6123478043851875 1106 68-69 0.4652726644745968 1106 70-71 0.5379414793257844 1106 72-73 0.5358973921528687 1106 74-75 0.6162988047684586 1106 76-77 0.7005733976733985 1106 78-79 0.834312976108329 1106 80-81 0.7651926985967492 1106 82-83 0.5428826286614914 1106 84-85 0.3513773399489004 1106 86-87 0.7692154289684616 1106 88-89 0.7432059947545362 1106 90-91 1.045185026412149 1106 92-93 1.0344705799901064 1106 94-95 1.1459939514673998 1106 96-97 0.924974926533995 1106 98-99 1.3843721896420753 1106 100-101 1.8028978191435812 1107 1 0.2470658678858193 1107 2 0.1718289403344606 1107 3 0.27469621511717435 1107 4 0.33574339771927697 1107 5 0.3487939719578961 1107 6 0.18364461818349298 1107 7 0.27577957083789073 1107 8 0.35306620597341265 1107 9 0.2735822284180003 1107 10-11 0.2721947323615268 1107 12-13 0.3503119369438181 1107 14-15 0.4312917989581635 1107 16-17 0.47761671145403994 1107 18-19 0.5649180652057595 1107 20-21 0.3842089998803573 1107 22-23 0.3751277756510518 1107 24-25 0.44803004886347253 1107 26-27 0.35768707181154014 1107 28-29 0.43065610610123883 1107 30-31 0.48292715616668147 1107 32-33 0.4687245328300591 1107 34-35 0.6001719126157568 1107 36-37 0.46468573078458775 1107 38-39 0.5829361822423493 1107 40-41 0.5008214378070477 1107 42-43 0.6942694239290361 1107 44-45 0.6746038124387042 1107 46-47 0.6593465366889362 1107 48-49 0.9118416907665718 1107 50-51 0.8789791666388425 1107 52-53 1.060823737766185 1107 54-55 0.8864684153065241 1107 56-57 1.0286971312135051 1107 58-59 1.065713369548611 1107 60-61 0.9481648488071599 1107 62-63 0.8871704830700935 1107 64-65 0.7724591825378226 1107 66-67 0.8970169267140378 1107 68-69 0.9333552332644928 1107 70-71 1.2013361471936754 1107 72-73 1.354766171981467 1107 74-75 1.2493469429697832 1107 76-77 1.3381465496070426 1107 78-79 1.6080979270045859 1107 80-81 1.3877947691837313 1107 82-83 1.4982445062167038 1107 84-85 1.5012150229594035 1107 86-87 1.5992834158386522 1107 88-89 1.5027573232259606 1107 90-91 1.4348807485801984 1107 92-93 1.3631919440610325 1107 94-95 1.5800797589944189 1107 96-97 1.8503651810523536 1107 98-99 1.7794947921878297 1107 100-101 2.0930855290485297 1108 1 -0.02995008151483347 1108 2 -0.1822610940281031 1108 3 -0.06285400503215044 1108 4 -0.060515504803198894 1108 5 -0.06651873724983659 1108 6 -0.01479527368106659 1108 7 0.024736053982280737 1108 8 0.03128175715369963 1108 9 0.17347954421844491 1108 10-11 0.0015913988717386474 1108 12-13 0.056450673425850084 1108 14-15 -0.02840074132705439 1108 16-17 0.11010403238456945 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-0.514025749236275 2316 66-67 -0.5985762197105586 2316 68-69 -0.4196671625030888 2316 70-71 -0.20859376202973223 2316 72-73 -0.08896826344779996 2316 74-75 -0.2131302481201125 2316 76-77 -0.1967410411982371 2316 78-79 -0.43832322589257444 2316 80-81 -0.09969332427828803 2316 82-83 0.048271331790221694 2316 84-85 0.13620073511382813 2316 86-87 0.12020592866328528 2316 88-89 0.24939910570642354 2316 90-91 -0.06995144096040917 2316 92-93 0.03752732971809891 2316 94-95 0.30947284115240237 2316 96-97 0.2967821738338401 2316 98-99 0.1475495507863549 2316 100-101 0.5583263280202644 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4241.0 3 836.0 4 751.0 5 896.0 6 1039.0 7 1149.0 8 1500.0 9 1821.0 10 2235.0 11 2907.0 12 4012.0 13 5247.0 14 5865.0 15 7881.0 16 10208.0 17 14088.0 18 11776.0 19 7362.0 20 6891.0 21 6939.0 22 7413.0 23 8106.0 24 8863.0 25 10015.0 26 11653.0 27 13671.0 28 15867.0 29 19137.0 30 23270.0 31 29195.0 32 37552.0 33 49178.0 34 66984.0 35 94726.0 36 136733.0 37 184512.0 38 143264.0 39 18730.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.26046807833148 19.11941287343807 14.902393804150767 21.717725244079688 2 15.229504964323418 20.02684472779405 41.344988641825196 23.39866166605734 3 19.04143361903168 25.657812995766378 30.060449484582662 25.24030390061928 4 12.158954404049323 16.761260382550127 37.38436739596916 33.69541781743139 5 12.926352490405721 38.25699319725098 33.854610489744545 14.962043822598762 6 30.924816636580733 36.51125631265836 17.668464981101792 14.895462069659112 7 28.15887958838336 34.06242592347453 20.204330635536824 17.574363852605288 8 26.854512613148874 34.55879636281533 19.399412960036123 19.18727806399967 9 27.1435169030563 16.686713602495946 20.565898314825837 35.60387117962192 10-11 24.95520117903518 27.15444868200124 26.584841680043027 21.305508458920556 12-13 26.0159360096552 24.935267657843774 27.608510699437705 21.44028563306332 14-15 23.08204244847108 25.634410323589673 26.73551728012881 24.548029947810434 16-17 21.198539040339565 29.32628167499292 27.468832997409763 22.006346287257756 18-19 21.2564531083958 26.748977739373696 31.116079239886556 20.87848991234394 20-21 22.16554084906793 25.72820714359741 31.370138658725917 20.736113348608747 22-23 21.905817231358004 25.7336626831752 31.33399511105297 21.026524974413828 24-25 21.370728816906972 26.06436818168993 31.532713780393024 21.03218922101008 26-27 20.965987567289122 26.383390489325066 31.33754255915183 21.313079384233976 28-29 21.038339133585048 26.4346771238825 31.44374440030255 21.083239342229902 30-31 21.00890311252321 26.239783176806654 31.46471107088265 21.286602639787485 32-33 20.857630486230416 26.26745443834835 31.294425047773284 21.580490027647954 34-35 21.510485348220286 26.387006070059677 31.052793612839995 21.04971496888004 36-37 21.457136773951923 25.915148281443596 31.102446432190195 21.52526851241429 38-39 21.02049904471866 26.44959582672366 30.9596319242506 21.570273204307078 40-41 21.359695474179947 26.302699485958776 31.093850999866618 21.243754039994663 42-43 21.109032017415817 26.31289465083454 30.882699266764597 21.695374064985046 44-45 21.254093511895874 26.600144372771112 30.686447891126306 21.459314224206707 46-47 21.59128221966836 26.9544204564111 29.821303973075032 21.63299335084551 48-49 21.530572400972638 27.081153719654516 29.5371667852787 21.851107094094147 50-51 21.77363157273562 27.420629422661474 29.179892958122306 21.6258460464806 52-53 22.00317982973849 27.20858574557667 29.148610338449664 21.639624086235177 54-55 21.504558178862858 27.40812837604873 29.290147273712382 21.79716617137603 56-57 21.69569753592397 27.726061715261185 28.980925698141196 21.59731505067365 58-59 21.79937185407687 27.02355983210459 29.175239170945865 22.00182914287268 60-61 21.552270717359143 27.352074557254742 29.459974925978287 21.635679799407825 62-63 21.450869439193124 27.284871834992526 29.643836459757495 21.620422266056856 64-65 21.323135637561172 27.266730274024603 29.526279072153315 21.883855016260913 66-67 21.429571753292223 27.572181123410832 29.04029872261098 21.957948400685968 68-69 21.54116233567026 27.266962912498037 29.337622775975973 21.854251975855725 70-71 21.45023025186892 27.432264604206345 29.262942658384404 21.854562485540328 72-73 21.481742916752378 27.492609086194243 29.021372732388645 22.004275264664738 74-75 21.434163248303474 27.957764831660576 28.7259987810859 21.88207313895005 76-77 21.561883984652784 27.628169673137958 28.844987578359977 21.964958763849282 78-79 21.60163126830187 27.76542616319969 28.714416703054475 21.918525865443968 80-81 21.556052426624582 28.07654459577463 28.493919714163457 21.873483263437326 82-83 21.702943070202217 27.99505426710549 28.393380721389526 21.90862194130277 84-85 21.59983665713491 27.944968229197126 28.375487489188284 22.079707624479678 86-87 21.59545051122751 27.796668428045944 28.50038220220292 22.107498858523623 88-89 21.68281502117628 27.807799950233825 28.317518463507795 22.1918665650821 90-91 21.533645888807825 27.87787556680181 28.563168323333816 22.02531022105655 92-93 21.65331163557618 27.526122215079702 28.592865904680014 22.2277002446641 94-95 21.441214224369244 27.53947593889066 28.663292225069032 22.356017611671067 96-97 21.709658637679897 27.433075390056917 28.42133072055689 22.435935251706297 98-99 21.35214264790455 27.544687769174452 28.371622477808096 22.7315471051129 100-101 21.65515309789124 27.001922091432455 28.87584582025906 22.467078990417242 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1393.0 1 835.5 2 338.0 3 374.0 4 301.0 5 297.5 6 508.0 7 1091.0 8 1851.5 9 2221.0 10 2018.0 11 1735.0 12 1757.5 13 1965.0 14 2098.0 15 2097.5 16 2161.5 17 2241.5 18 2279.5 19 2412.5 20 2693.0 21 3064.5 22 3460.5 23 4043.5 24 4858.0 25 5866.0 26 7378.5 27 8942.0 28 9852.0 29 10862.5 30 12442.5 31 14567.5 32 17288.0 33 19415.5 34 21311.0 35 23060.0 36 25455.5 37 28303.0 38 29198.5 39 29285.5 40 29584.5 41 30525.5 42 31374.0 43 32278.5 44 33969.5 45 35325.5 46 35387.0 47 35229.0 48 35450.0 49 34753.0 50 33993.5 51 32261.0 52 29896.0 53 29271.0 54 29302.5 55 27170.5 56 23893.5 57 21446.5 58 19629.0 59 17360.5 60 14282.0 61 11898.0 62 9985.0 63 7653.5 64 5792.5 65 4676.5 66 3405.0 67 2494.5 68 1937.0 69 1389.5 70 1012.5 71 744.0 72 564.5 73 468.0 74 384.0 75 314.0 76 244.5 77 156.5 78 109.5 79 73.0 80 53.5 81 48.5 82 31.5 83 17.5 84 9.5 85 6.0 86 6.5 87 8.0 88 6.5 89 3.0 90 2.0 91 3.5 92 3.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30311874369569336 2 0.2833545823668863 3 0.27075876970655854 4 0.2585725769701438 5 0.25570523750275215 6 0.21341198035872466 7 0.20644844165220197 8 0.21863463438861666 9 0.21863463438861666 10-11 0.22109235393209525 12-13 0.21689374971198597 14-15 0.21366799281117033 16-17 0.21371919530165945 18-19 0.21315596790627897 20-21 0.20112338264133164 22-23 0.19124130197692818 24-25 0.19328940159649366 26-27 0.20368350716578856 28-29 0.21832741944568185 30-31 0.21305356292530067 32-33 0.2172009646549208 34-35 0.21095426081524604 36-37 0.197897625740516 38-39 0.19794882823100513 40-41 0.19303338914404797 42-43 0.20465635448508215 44-45 0.20015053532203808 46-47 0.2002017378125272 48-49 0.20987900851497415 50-51 0.21812260948372528 52-53 0.23030880222014 54-55 0.2051171768994844 56-57 0.1790039067500243 58-59 0.17833827437366553 60-61 0.18443137074187288 62-63 0.19380142650138504 64-65 0.18279289104622048 66-67 0.18755472266171028 68-69 0.19190693435328696 70-71 0.18653067285192754 72-73 0.18827155752855818 74-75 0.19221414929622177 76-77 0.2054243918424192 78-79 0.21080065334377865 80-81 0.2019426224891579 82-83 0.1984096506454074 84-85 0.19231655427720004 86-87 0.1945694638587221 88-89 0.20045775026497287 90-91 0.20153300256524476 92-93 0.1959519311019288 94-95 0.19805123321198345 96-97 0.1861210529280144 98-99 0.19369902152040674 100-101 0.18386814334649237 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 976515.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.605953899451606 #Duplication Level Percentage of deduplicated Percentage of total 1 79.27557448810887 43.289183658502154 2 11.665918684199864 12.74057235728338 3 3.586589320946512 5.875473933476121 4 1.604992463749687 3.505685779379307 5 0.8453991433618713 2.3081913324527115 6 0.5489214842082895 1.7984628756657846 7 0.36208300983823943 1.3840321700101113 8 0.2861965750055978 1.2502429584749295 9 0.22710840739515442 1.116132410195791 >10 1.4228996453398357 14.851868630222576 >50 0.1205992110951867 4.4751125752918055 >100 0.051275859253901955 5.013171981699128 >500 0.0015025892288957996 0.5154811238583977 >1k 5.634709608359249E-4 0.6502487714409554 >5k 3.756473072239499E-4 1.2261394420467857 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6700 0.686113372554441 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5255 0.5381381750408339 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3581 0.36671223688320204 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1417 0.14510785804621537 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1342 0.13742748447284475 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.0240498097827478E-4 0.0 0.0 0.0 0.0 8 1.0240498097827478E-4 0.0 0.0 0.0 0.0 9 1.0240498097827478E-4 0.0 0.0 0.0 0.0 10-11 1.0240498097827478E-4 0.0 0.0 0.0 0.0 12-13 1.0240498097827478E-4 0.0 0.0 0.0 0.0 14-15 1.5360747146741217E-4 0.0 0.0 0.0 0.0 16-17 2.0480996195654957E-4 0.0 0.0 0.0 0.0 18-19 2.0480996195654957E-4 0.0 0.0 0.0 0.0 20-21 2.0480996195654957E-4 0.0 0.0 0.0 0.0 22-23 2.0480996195654957E-4 0.0 0.0 1.0240498097827478E-4 0.0 24-25 3.0721494293482434E-4 0.0 0.0 1.0240498097827478E-4 0.0 26-27 3.5841743342396174E-4 0.0 0.0 2.0480996195654957E-4 0.0 28-29 5.120249048913739E-4 0.0 0.0 5.120249048913739E-4 0.0 30-31 7.168348668479235E-4 0.0 0.0 0.0013312647527175723 0.0 32-33 7.680373573370608E-4 0.0 0.0 0.0023041120720111827 0.0 34-35 8.192398478261983E-4 0.0 0.0 0.0029697444483699686 0.0 36-37 0.0010240498097827478 0.0 0.0 0.0037377818057070297 0.0 38-39 0.0010240498097827478 0.0 0.0 0.005478666482337701 0.0 40-41 0.0010240498097827478 0.0 0.0 0.007680373573370609 0.0 42-43 0.0010240498097827478 0.0 0.0 0.01157176285054505 0.0 44-45 0.0010240498097827478 0.0 0.0 0.019354541404893934 0.0 46-47 0.00117765728125016 0.0 0.0 0.0345104785896786 0.0 48-49 0.0012288597717392974 0.0 0.0 0.04838635351223484 0.0 50-51 0.0012288597717392974 0.0 0.0 0.06763848993615049 0.0 52-53 0.0012288597717392974 0.0 0.0 0.09277891276631695 0.0 54-55 0.0012288597717392974 0.0 0.0 0.1163832608818093 0.0 56-57 0.0012288597717392974 0.0 0.0 0.1484360199280093 0.0 58-59 0.0012288597717392974 0.0 0.0 0.19190693435328693 0.0 60-61 0.0012288597717392974 0.0 0.0 0.24556714438590294 0.0 62-63 0.0012288597717392974 0.0 0.0 0.31136234466444446 0.0 64-65 0.0012288597717392974 0.0 0.0 0.3714740684986918 0.0 66-67 0.0012288597717392974 0.0 0.0 0.4500186889090285 0.0 68-69 0.0012288597717392974 0.0 0.0 0.5494027229484442 0.0 70-71 0.0012288597717392974 0.0 0.0 0.6353717044797059 0.0 72-73 0.0012288597717392974 0.0 0.0 0.7514477504185804 0.0 74-75 0.0012288597717392974 0.0 0.0 0.861993927384628 0.0 76-77 0.0012288597717392974 0.0 0.0 0.9787868081903504 0.0 78-79 0.0012288597717392974 0.0 0.0 1.1220513765789568 0.0 80-81 0.001433669733695847 0.0 0.0 1.263319047838487 0.0 82-83 0.001433669733695847 0.0 0.0 1.4353594158819885 0.0 84-85 0.001433669733695847 0.0 0.0 1.6427807048534842 0.0 86-87 0.001433669733695847 0.0 0.0 1.8705293825491673 0.0 88-89 0.001433669733695847 0.0 0.0 2.1527575101252925 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2825 0.0 55.708836 1 GTATCAA 5600 0.0 46.1027 1 ATCAACG 7225 0.0 35.13048 3 TCAACGC 7335 0.0 34.601868 4 CAACGCA 7380 0.0 34.262074 5 TATCAAC 7460 0.0 34.22024 2 AACGCAG 7600 0.0 33.319126 6 ACGCAGA 8655 0.0 29.147913 7 CGCAGAG 8625 0.0 29.139128 8 TGGTATC 1245 0.0 29.019629 2 GTACATG 6330 0.0 27.19064 1 GCAGAGT 9190 0.0 26.9327 9 TACATGG 6465 0.0 26.398151 2 ACATGGG 6300 0.0 25.95366 3 GTGGTAT 1540 0.0 24.390512 1 GAGTACT 5500 0.0 23.922573 12-13 ATGGGTA 340 0.0 23.764484 5 GTACTTT 5700 0.0 23.040333 14-15 CATGGGA 3350 0.0 22.558525 4 AGTACTT 5785 0.0 22.292421 12-13 >>END_MODULE