FastQCFastQC Report
Thu 26 May 2016
SRR937557_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937557_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976515
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71260.7297378944511861No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62050.635422906970195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42180.43194420976636305No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16890.17296201287230611No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10390.1063987752364275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34150.054.2728581
GTATCAA61450.042.767321
ATCAACG81000.032.0163463
TATCAAC83650.031.3427642
TCAACGC82850.031.3014344
CAACGCA83750.030.9650615
AACGCAG85750.030.2982336
GTAAGGT4100.030.1199054
GCGACAG2500.028.5039027
TGCGCGA350.00839576327.14101434-35
TGGTATC14700.027.1410142
ACGCAGA96400.026.8579317
CGCAGAG96450.026.8440078
TAAGGTG4850.026.4415045
GCAGAGT102450.025.0400319
GGTAAGG4450.024.5488953
GGACCGA3300.024.4680356
GAGTACT62300.023.82950612-13
CGCGGAA1102.1700544E-923.7508282-83
AGGCGAC3000.023.7483865
Produced by FastQC (version 0.11.5)