##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937557_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 976515 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27431939089517 33.0 31.0 34.0 31.0 34.0 2 32.48095113746333 34.0 31.0 34.0 31.0 34.0 3 32.52613631127018 34.0 31.0 34.0 31.0 34.0 4 36.135245234328195 37.0 35.0 37.0 35.0 37.0 5 36.06136516080142 37.0 35.0 37.0 35.0 37.0 6 36.12201963103485 37.0 35.0 37.0 35.0 37.0 7 36.0792215173346 37.0 35.0 37.0 35.0 37.0 8 36.04941347547145 37.0 35.0 37.0 35.0 37.0 9 37.73730255039605 39.0 37.0 39.0 35.0 39.0 10-11 37.65682349989503 39.0 37.0 39.0 35.0 39.0 12-13 37.55171553944383 39.0 37.0 39.0 35.0 39.0 14-15 38.75163924773301 40.0 38.0 41.0 35.0 41.0 16-17 38.71495471139716 40.0 38.0 41.0 34.5 41.0 18-19 38.725912556386746 40.0 38.0 41.0 35.0 41.0 20-21 38.64589944854917 40.0 38.0 41.0 34.0 41.0 22-23 38.591985786188644 40.0 38.0 41.0 34.0 41.0 24-25 38.592792225413845 40.0 38.0 41.0 34.0 41.0 26-27 38.40315407341413 40.0 38.0 41.0 34.0 41.0 28-29 38.36666154641762 40.0 38.0 41.0 34.0 41.0 30-31 38.32194436337383 40.0 38.0 41.0 34.0 41.0 32-33 38.143738191425626 40.0 37.0 41.0 34.0 41.0 34-35 37.97287855281281 40.0 37.0 41.0 33.5 41.0 36-37 37.772601547339264 40.0 37.0 41.0 33.0 41.0 38-39 37.606296882280354 40.0 37.0 41.0 33.0 41.0 40-41 37.4027321648925 40.0 36.5 41.0 33.0 41.0 42-43 37.125338064443454 40.0 36.0 41.0 32.0 41.0 44-45 36.73163750684833 40.0 36.0 41.0 31.0 41.0 46-47 36.270685038120256 39.0 35.5 41.0 30.0 41.0 48-49 35.83119306923089 39.0 35.0 41.0 28.5 41.0 50-51 35.542661403050644 39.0 35.0 41.0 27.5 41.0 52-53 35.25964219699647 39.0 35.0 40.0 27.0 41.0 54-55 35.01314931158252 38.5 35.0 40.0 26.0 41.0 56-57 34.726976544139106 38.0 34.0 40.0 26.0 41.0 58-59 34.46370613866658 38.0 34.0 40.0 25.0 41.0 60-61 34.11746926570508 38.0 34.0 40.0 23.5 41.0 62-63 33.70847196407633 37.0 33.5 40.0 22.5 41.0 64-65 33.314185649990016 36.5 33.0 39.0 22.0 41.0 66-67 32.891502946703326 36.0 32.5 39.0 20.5 40.0 68-69 32.41580262463966 35.5 32.0 39.0 18.5 40.0 70-71 31.993247415554293 35.0 31.5 38.0 18.0 40.0 72-73 31.530744535414204 35.0 31.0 37.5 14.5 39.5 74-75 31.052340209827804 35.0 31.0 37.0 10.0 39.0 76-77 29.81668484355079 33.5 29.5 35.5 8.0 38.0 78-79 30.407149403747 34.0 30.5 36.0 7.0 38.0 80-81 30.239032682549677 34.0 30.5 35.5 6.0 37.0 82-83 29.854592095359518 34.0 30.0 35.0 2.0 37.0 84-85 29.45008986037081 34.0 29.5 35.0 2.0 36.5 86-87 29.120968443905113 34.0 29.0 35.0 2.0 36.0 88-89 28.723205480714583 34.0 29.0 35.0 2.0 36.0 90-91 28.22187677608639 34.0 29.0 35.0 2.0 35.0 92-93 27.766785968469506 33.0 27.0 35.0 2.0 35.0 94-95 27.26998151590093 33.0 26.5 35.0 2.0 35.0 96-97 26.745615274726962 33.0 25.5 35.0 2.0 35.0 98-99 25.926688786142556 32.5 24.5 34.0 2.0 35.0 100-101 23.498448052513275 30.5 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11803151977412085 1101 2 0.14997448868331276 1101 3 0.1129142337285245 1101 4 0.04599957122321996 1101 5 0.12981462358533236 1101 6 0.14972952615344326 1101 7 0.09034149894500132 1101 8 0.025244132112824502 1101 9 0.1361495205567138 1101 10-11 0.14027001528419447 1101 12-13 0.20974529127344255 1101 14-15 0.24452672059025815 1101 16-17 0.2284744901621778 1101 18-19 0.12555342145945758 1101 20-21 0.15553641822631192 1101 22-23 0.125565953063429 1101 24-25 0.04415572624233022 1101 26-27 0.12514527971701028 1101 28-29 -8.45915900207217E-4 1101 30-31 0.3488884062683937 1101 32-33 0.19619147007447424 1101 34-35 0.13997514922407817 1101 36-37 0.14375324150652347 1101 38-39 0.16787123171410911 1101 40-41 0.17524673084898268 1101 42-43 0.23179138328519144 1101 44-45 0.28964502838748274 1101 46-47 0.32989740848555016 1101 48-49 0.5695718988826286 1101 50-51 0.4766291138225256 1101 52-53 0.4308109237365869 1101 54-55 0.3956834799937283 1101 56-57 0.3721876427796289 1101 58-59 0.2338881706179663 1101 60-61 0.1948623994350811 1101 62-63 0.34047634177972697 1101 64-65 0.30814045845160365 1101 66-67 0.3563162637508839 1101 68-69 0.4018032370970772 1101 70-71 0.32669039428533964 1101 72-73 0.28161166685691796 1101 74-75 0.4562186316476371 1101 76-77 0.16493562637718284 1101 78-79 0.2649866937794201 1101 80-81 0.2120661337786771 1101 82-83 0.41569169237993364 1101 84-85 0.5981620148946725 1101 86-87 0.5868769396610389 1101 88-89 0.4707501612597902 1101 90-91 0.5869361997942129 1101 92-93 0.8376953536375424 1101 94-95 0.6485018914374692 1101 96-97 0.599783735455901 1101 98-99 0.7959197559387832 1101 100-101 1.1846598364573744 1102 1 0.09181176511305722 1102 2 0.16685185672923097 1102 3 0.14263925905020614 1102 4 0.09039166335661974 1102 5 0.08352359308678103 1102 6 0.16411073934322928 1102 7 0.2081402009744835 1102 8 0.1636167922021059 1102 9 0.15008432192221477 1102 10-11 0.1954457664940037 1102 12-13 0.21859587460989616 1102 14-15 0.20842700944496784 1102 16-17 0.23897754752047007 1102 18-19 0.17688290339665969 1102 20-21 0.2525788461064167 1102 22-23 0.20161201976260656 1102 24-25 0.1325351387793745 1102 26-27 0.09473750689936367 1102 28-29 0.23664517462011503 1102 30-31 0.1385489082003417 1102 32-33 0.08545057650055554 1102 34-35 0.31932128719909514 1102 36-37 0.3038335206076894 1102 38-39 0.35707331747020277 1102 40-41 0.40778752688754594 1102 42-43 0.3552129986207504 1102 44-45 0.3766379383173373 1102 46-47 0.19904960115539438 1102 48-49 0.30351457810385085 1102 50-51 0.2604074834815293 1102 52-53 0.4475532428272615 1102 54-55 0.42192386713406904 1102 56-57 0.45478470921620584 1102 58-59 0.429131446305405 1102 60-61 0.5866971895464914 1102 62-63 0.4242704671501727 1102 64-65 0.40481862928853474 1102 66-67 0.18570591750037124 1102 68-69 0.36904973895399706 1102 70-71 0.37937899390270147 1102 72-73 0.3373471209590271 1102 74-75 0.2096094858322779 1102 76-77 0.2063446371435873 1102 78-79 0.19912444371376736 1102 80-81 0.29287830319008634 1102 82-83 0.36425342100656266 1102 84-85 0.2921665915537126 1102 86-87 0.3900993577876086 1102 88-89 0.384402485227227 1102 90-91 0.27992228224909965 1102 92-93 0.5971007630984708 1102 94-95 0.7044376681565296 1102 96-97 0.7655364458134031 1102 98-99 0.6959782771527259 1102 100-101 0.9216872588980074 1103 1 0.10638616598043171 1103 2 0.10913902406267795 1103 3 0.24660562332784508 1103 4 0.18499360361878558 1103 5 0.10660916748985017 1103 6 0.08070991831031193 1103 7 0.11185897550083723 1103 8 0.16369255496022106 1103 9 0.07045472089772176 1103 10-11 0.10956242790141602 1103 12-13 0.18013744813618615 1103 14-15 0.11529398401737012 1103 16-17 0.1719698013301283 1103 18-19 0.08617575735033967 1103 20-21 0.18029345147439102 1103 22-23 0.3384730289372584 1103 24-25 0.08317533408546751 1103 26-27 0.06280938883892162 1103 28-29 -0.025398345567715808 1103 30-31 0.0477119356801623 1103 32-33 0.21811815379143695 1103 34-35 0.3329589514406166 1103 36-37 0.3591326106454815 1103 38-39 0.19927788133048097 1103 40-41 0.21425781354292894 1103 42-43 0.31103264500728045 1103 44-45 0.22513522446591594 1103 46-47 0.1599144587839305 1103 48-49 0.20698877867802423 1103 50-51 0.4792037088779466 1103 52-53 0.2702823644867962 1103 54-55 0.17668092244897338 1103 56-57 0.26162498293307834 1103 58-59 0.25298450480381973 1103 60-61 0.2438479066814523 1103 62-63 0.185574380995412 1103 64-65 0.3958216178718956 1103 66-67 0.2924287957201912 1103 68-69 0.14026018509366622 1103 70-71 0.21354461423419124 1103 72-73 0.23758779643918615 1103 74-75 0.24774463335266717 1103 76-77 0.36294074146669786 1103 78-79 0.5523609478288662 1103 80-81 0.6960303281520765 1103 82-83 0.664276517614379 1103 84-85 0.5283580933260481 1103 86-87 0.4807388322441568 1103 88-89 0.7959590274149484 1103 90-91 0.898760581720893 1103 92-93 0.8754873399973029 1103 94-95 0.9397380380700362 1103 96-97 1.2266635308523206 1103 98-99 1.1510263203036644 1103 100-101 1.7610963240959059 1104 1 0.16462011947948696 1104 2 0.13902370864327196 1104 3 0.15842550936785926 1104 4 0.03716188754550842 1104 5 0.05841281797206932 1104 6 0.08695009050202884 1104 7 0.14927852912669692 1104 8 0.10511418839658404 1104 9 0.11975319222218417 1104 10-11 0.1791201644928222 1104 12-13 0.06617523876710862 1104 14-15 0.11856479712106704 1104 16-17 0.03653251154245396 1104 18-19 0.23212653368700842 1104 20-21 0.2274036036605267 1104 22-23 0.25787963115053714 1104 24-25 0.22042097763053192 1104 26-27 0.3262513122784867 1104 28-29 0.25557383252272103 1104 30-31 0.27187484528172945 1104 32-33 0.33449162494925133 1104 34-35 0.2758822999059021 1104 36-37 0.24897894316480773 1104 38-39 0.30101972262938403 1104 40-41 0.36199398276603034 1104 42-43 0.3361977595931336 1104 44-45 0.47516725858429254 1104 46-47 0.1821850222807342 1104 48-49 0.08884398742566901 1104 50-51 -0.024635570950636065 1104 52-53 0.1335722277066722 1104 54-55 0.110415848822349 1104 56-57 0.1206411614590337 1104 58-59 0.012658993720748413 1104 60-61 -0.07122153513977025 1104 62-63 -0.07740980637121453 1104 64-65 -0.019878655362468578 1104 66-67 -0.05045440825455927 1104 68-69 -0.11276972403313579 1104 70-71 -0.1683317037012877 1104 72-73 -0.27074885140697447 1104 74-75 0.03138804954511798 1104 76-77 0.0442186694777007 1104 78-79 -0.003604323483454408 1104 80-81 0.08875899065171566 1104 82-83 -0.02630241482362905 1104 84-85 -4.6224378397852206E-4 1104 86-87 -0.03881716376509203 1104 88-89 -0.08370267748156834 1104 90-91 -0.012815622817509364 1104 92-93 -0.036843610590160836 1104 94-95 0.19028484010214797 1104 96-97 0.24324424238749032 1104 98-99 0.48118549839336566 1104 100-101 0.9376972708244615 1105 1 0.1696964951858817 1105 2 0.17129991214752494 1105 3 0.22799034019952558 1105 4 0.1646694642838682 1105 5 0.1447205637929656 1105 6 0.14104792440495117 1105 7 0.13691709803952534 1105 8 0.06878483931756563 1105 9 0.2020366488058798 1105 10-11 0.1260915748814142 1105 12-13 0.1199505836003425 1105 14-15 0.2143276728968715 1105 16-17 0.33602191034795936 1105 18-19 0.30556825246407016 1105 20-21 0.27906541674320806 1105 22-23 0.3871911189365065 1105 24-25 0.27734517281168536 1105 26-27 0.23463400034771809 1105 28-29 0.27966692462747034 1105 30-31 0.12414326537385278 1105 32-33 0.3531885819314695 1105 34-35 0.4245813470228441 1105 36-37 0.4174227442775802 1105 38-39 0.4356980992498265 1105 40-41 0.43417031214630697 1105 42-43 0.5978752174901771 1105 44-45 0.7233980531318451 1105 46-47 0.829547709008537 1105 48-49 0.8320869250320371 1105 50-51 0.7826465987963829 1105 52-53 0.7381561739122162 1105 54-55 0.7658356929357311 1105 56-57 0.9395844111817837 1105 58-59 0.9550301579725868 1105 60-61 0.9192375457936777 1105 62-63 0.8212038415845839 1105 64-65 0.6782481783797891 1105 66-67 0.672698279206351 1105 68-69 0.6162064843065096 1105 70-71 0.7429701538669455 1105 72-73 0.7803643013116783 1105 74-75 0.8128434072311173 1105 76-77 0.8296823284301063 1105 78-79 0.855056165327305 1105 80-81 0.8003558847739143 1105 82-83 0.7150344447021588 1105 84-85 0.7266133457821944 1105 86-87 1.0040770021073762 1105 88-89 1.0998415670829118 1105 90-91 1.276179037980146 1105 92-93 1.2406545293459175 1105 94-95 1.4592574920931547 1105 96-97 1.518159350162012 1105 98-99 1.4815828579604862 1105 100-101 1.7943015506092728 1106 1 -0.2574420013269574 1106 2 -0.2436815135042707 1106 3 -0.21115467521322984 1106 4 -0.23204436278462026 1106 5 -0.22557139409764915 1106 6 -0.2970319358828277 1106 7 -0.18634430909438038 1106 8 -0.3409667875845628 1106 9 -0.22868686628130774 1106 10-11 -0.4136484971576948 1106 12-13 -0.5697834663603274 1106 14-15 -0.53114824067125 1106 16-17 -0.35187916948597575 1106 18-19 -0.4488363333486731 1106 20-21 -0.3632786396535579 1106 22-23 -0.5017259984450959 1106 24-25 -0.4513743074829435 1106 26-27 -0.39657015187536615 1106 28-29 -0.41045761948237214 1106 30-31 -0.6149991153795824 1106 32-33 -0.5694916946324824 1106 34-35 -0.6436826759513892 1106 36-37 -0.6476486361709917 1106 38-39 -0.5881737960864584 1106 40-41 -0.657492308436403 1106 42-43 -0.8731990460284536 1106 44-45 -0.8875803840635186 1106 46-47 -0.7609584843227708 1106 48-49 -0.836828611553976 1106 50-51 -0.7028176093567566 1106 52-53 -0.8465885840566827 1106 54-55 -0.8682195370512105 1106 56-57 -0.9009989400296021 1106 58-59 -0.9910411886017982 1106 60-61 -1.1327358140368062 1106 62-63 -1.0919728424332007 1106 64-65 -1.4305387868314128 1106 66-67 -1.3136664178642832 1106 68-69 -1.0813186296425101 1106 70-71 -1.0606206985958941 1106 72-73 -0.9722421295153367 1106 74-75 -1.0101545339708125 1106 76-77 -1.0108897313841858 1106 78-79 -0.6961672575468043 1106 80-81 -1.115923110592849 1106 82-83 -1.2744523236623557 1106 84-85 -0.9435169569401616 1106 86-87 -0.6892373616788596 1106 88-89 -0.9707146094994847 1106 90-91 -1.2646233663346749 1106 92-93 -0.9531933529525567 1106 94-95 -0.9390935875525948 1106 96-97 -1.0397514026354315 1106 98-99 -1.12987734551049 1106 100-101 -0.7312028925554834 1107 1 0.09781077792383286 1107 2 0.07055866622282991 1107 3 0.028577444734082746 1107 4 0.1322118775813692 1107 5 0.07416954494605221 1107 6 0.18887651657482962 1107 7 0.11362661069556168 1107 8 0.1950261495356571 1107 9 0.2521895694352665 1107 10-11 0.1754680916766489 1107 12-13 0.20948158359871627 1107 14-15 0.0987082019257528 1107 16-17 0.2116886491751515 1107 18-19 0.21414303870885476 1107 20-21 0.19379649942777633 1107 22-23 0.28352330258449854 1107 24-25 0.11403668331592343 1107 26-27 0.34660217592892195 1107 28-29 0.18860183529219654 1107 30-31 0.2915018321030729 1107 32-33 0.42636057409907124 1107 34-35 0.3510638795642578 1107 36-37 0.46479151656663475 1107 38-39 0.49340712197388115 1107 40-41 0.4640354099455166 1107 42-43 0.570801592595771 1107 44-45 0.6335289024069652 1107 46-47 0.6275374415968287 1107 48-49 0.7299869723975476 1107 50-51 0.622518191042758 1107 52-53 0.7410754197750578 1107 54-55 0.7996934922133718 1107 56-57 0.8163490208394606 1107 58-59 0.9141554259295646 1107 60-61 0.8352455442180826 1107 62-63 0.7274998744376973 1107 64-65 0.8347497059584015 1107 66-67 0.8056118607393401 1107 68-69 0.7647710324378423 1107 70-71 0.9152764638978574 1107 72-73 0.9761238667859615 1107 74-75 1.1194643252106466 1107 76-77 0.9359467028878861 1107 78-79 1.0169978097083074 1107 80-81 1.113286915047155 1107 82-83 0.8676909828525616 1107 84-85 1.0014045550026793 1107 86-87 1.0548496622752523 1107 88-89 1.064345099964509 1107 90-91 1.268055282138807 1107 92-93 1.0556775366167699 1107 94-95 1.2293430755598216 1107 96-97 1.2876504231455996 1107 98-99 1.4719661117273475 1107 100-101 1.591671543659075 1108 1 0.1266142311432361 1108 2 0.049713856056847305 1108 3 0.0340855009559462 1108 4 0.054570435654760274 1108 5 0.057144718755729684 1108 6 0.08042652098028213 1108 7 0.028588941980977722 1108 8 -0.14695505439746626 1108 9 -0.05210068676468893 1108 10-11 -0.0224089721975389 1108 12-13 -0.011683054499179946 1108 14-15 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2316 40-41 -0.1429068189583944 2316 42-43 0.07125203270732072 2316 44-45 -0.09198953407811672 2316 46-47 0.009934557632490737 2316 48-49 0.14573315369825934 2316 50-51 0.2298376994031628 2316 52-53 0.27900850216256146 2316 54-55 0.349317216922735 2316 56-57 0.31766232632393354 2316 58-59 0.39024480242864 2316 60-61 0.5485511721373371 2316 62-63 0.5647539972557851 2316 64-65 0.6972227369326163 2316 66-67 0.7313107601735638 2316 68-69 0.8506351028097896 2316 70-71 0.8964454850943646 2316 72-73 0.7045285628393003 2316 74-75 0.6225793911413078 2316 76-77 0.5717751970981517 2316 78-79 0.6697321533281269 2316 80-81 0.6194051541419121 2316 82-83 0.5633856934806509 2316 84-85 0.4526845795617085 2316 86-87 0.5624892126384715 2316 88-89 0.644425175018732 2316 90-91 0.784455462314849 2316 92-93 0.9442968946391943 2316 94-95 0.9621947918227214 2316 96-97 1.3351689858686697 2316 98-99 1.2867893211561778 2316 100-101 1.7851771727259482 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 8.0 5 30.0 6 57.0 7 129.0 8 292.0 9 575.0 10 965.0 11 1667.0 12 2555.0 13 3774.0 14 5013.0 15 7001.0 16 9717.0 17 13966.0 18 12528.0 19 7244.0 20 5668.0 21 5361.0 22 5365.0 23 5716.0 24 6498.0 25 7789.0 26 9024.0 27 11202.0 28 13966.0 29 17342.0 30 21591.0 31 27497.0 32 34815.0 33 46663.0 34 65490.0 35 94276.0 36 144526.0 37 214918.0 38 160434.0 39 12847.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.31110291556539 19.95287561210482 15.565902432028192 21.170119040301596 2 15.830376389507586 20.805824795318046 40.05458185486142 23.309216960312952 3 19.413629078918397 25.490033435226287 30.399942653210655 24.69639483264466 4 12.853565997450117 17.391437919540405 36.69610809869792 33.05888798431156 5 12.963776598631933 38.611837408965265 33.68973989490103 14.734646097501775 6 31.217748831303155 36.90081565567349 17.053706292274057 14.827729220749298 7 28.16218900887339 34.269826884379654 19.947875864682057 17.620108242064894 8 27.232044566647723 34.849131861773756 18.93693389246453 18.98188967911399 9 27.332811067930347 16.520790771263112 20.330460873616893 35.81593728718965 10-11 25.14559428170586 27.450423188583894 26.18582407848318 21.21815845122707 12-13 26.172972513509237 25.144002093272494 27.467647213945455 21.21537817927281 14-15 23.063649815927047 25.930170043470913 26.624424612013126 24.38175552858891 16-17 21.301004080838492 29.52161513135999 27.36302053731893 21.814360250482583 18-19 21.2472414658249 26.813259396937067 31.34805916959801 20.591439967640028 20-21 21.983475898426338 25.786804681555697 31.759433822181578 20.470285597836384 22-23 21.879067481144297 25.75420253047891 31.706153129304298 20.6605768590725 24-25 21.522659662165967 26.068263160320114 31.780259391816816 20.628817785697095 26-27 21.05204948062142 26.520232646375565 31.59568808509135 20.832029787911665 28-29 20.890777274447245 26.567198722369945 31.759132530564653 20.782891472618154 30-31 21.065677434550416 26.252233708647587 31.755016564005672 20.92707229279632 32-33 20.936186336103386 26.336359400521243 31.52900877098662 21.19844549238875 34-35 21.637250835880657 26.369897031791627 31.295986236770556 20.69686589555716 36-37 21.577091947107693 26.088964554985218 31.219416034100945 21.114527463806144 38-39 21.162603749046355 26.503023507063382 31.23945868727055 21.094914056619714 40-41 21.42754006095163 26.401877293878623 31.311340772054002 20.859241873115742 42-43 21.2889589721187 26.460835020401014 31.030436869014068 21.21976913846622 44-45 21.444831876622477 26.748181031525377 30.784678166746033 21.022308925106117 46-47 21.86971014270134 27.20250072963549 29.784847134964643 21.142941992698525 48-49 21.798999503335843 27.352319216806702 29.51296191046732 21.335719369390127 50-51 22.020808692134786 27.529275023937167 29.153827642176516 21.296088641751535 52-53 22.193105072630733 27.605617937256465 29.04072134068601 21.160555649426787 54-55 21.83903985089835 27.733112138574423 29.11691064653385 21.310937363993386 56-57 22.001095733296467 28.114724300189962 28.797867928296032 21.08631203821754 58-59 22.00273421299212 27.449552746245576 28.872930779353105 21.674782261409195 60-61 21.728544876422788 27.703312289109743 29.378196955499913 21.189945878967553 62-63 21.671095682093977 27.615960840335273 29.495245848758085 21.217697628812665 64-65 21.656502972304573 27.50643871317901 29.29140873412083 21.54564958039559 66-67 21.580364868947225 27.94688253636657 28.903293856213164 21.56945873847304 68-69 21.81692037500704 27.55093367741407 29.13867170499173 21.49347424258716 70-71 21.72782804155594 27.863422476869275 28.950860969877574 21.45788851169721 72-73 21.841497570441824 27.666241685995608 28.827462967798755 21.664797775763812 74-75 21.820197334398344 28.25542874405411 28.29101447494406 21.633359446603485 76-77 21.86105692180868 27.886156382646455 28.705549837944115 21.547236857600755 78-79 21.904630241214935 27.98062497759891 28.492752287471262 21.621992493714895 80-81 21.77196458835758 28.3770858614563 28.396542807842174 21.45440674234395 82-83 21.920280672003 28.33673062379578 28.140931999404 21.60205670479722 84-85 21.815900974129875 28.19185622303862 28.131129913851588 21.861112888979918 86-87 21.752098022047793 28.157324772276926 28.236227810120685 21.8543493955546 88-89 21.78312673128421 28.07913856930001 28.274578475497048 21.863156223918732 90-91 21.75113324225063 28.17517784682948 28.311238608687816 21.76245030223207 92-93 21.771021400050998 27.856779311270024 28.482078212542227 21.890121076136744 94-95 21.534282627507 27.84447755538829 28.392651418565002 22.228588398539706 96-97 21.886709369543738 27.794299114708938 28.099619565495665 22.21937195025166 98-99 21.455390767608034 27.854931989769664 28.136650256142424 22.55302698647988 100-101 21.44624611829869 27.73019820233189 28.506128786451125 22.317426892918302 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 477.0 1 344.5 2 284.0 3 352.0 4 312.5 5 293.5 6 487.0 7 1076.0 8 1808.0 9 2231.0 10 2157.0 11 1923.0 12 1920.0 13 2069.5 14 2219.5 15 2311.0 16 2391.5 17 2481.5 18 2573.5 19 2655.5 20 2812.5 21 3179.5 22 3634.5 23 4240.0 24 4982.0 25 5927.5 26 7460.0 27 8949.5 28 9887.5 29 11089.5 30 12759.0 31 14715.0 32 17277.0 33 19365.5 34 21171.5 35 22642.0 36 25368.0 37 28485.5 38 29456.0 39 29381.0 40 29337.0 41 30667.5 42 31586.5 43 32269.5 44 33976.5 45 35397.0 46 34998.0 47 34572.5 48 35257.0 49 34917.0 50 34267.0 51 32526.5 52 30005.0 53 29448.5 54 29293.5 55 26862.5 56 23736.0 57 21376.5 58 19507.5 59 17296.5 60 14189.0 61 11616.5 62 9800.0 63 7588.0 64 5686.0 65 4469.5 66 3209.5 67 2463.5 68 1926.0 69 1313.5 70 910.5 71 681.5 72 568.5 73 500.0 74 414.0 75 325.0 76 224.0 77 140.5 78 92.5 79 68.5 80 44.5 81 27.0 82 21.5 83 12.0 84 5.0 85 4.0 86 2.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03840186786685304 2 0.0 3 0.0 4 0.0 5 0.010445308059784027 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005478666482337701 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.0480996195654957E-4 22-23 0.0020480996195654955 24-25 0.0 26-27 0.00117765728125016 28-29 0.001996897129076358 30-31 0.0 32-33 0.0 34-35 0.0 36-37 2.5601245244568694E-4 38-39 0.0 40-41 3.0721494293482434E-4 42-43 0.022068273400818215 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.5360747146741217E-4 84-85 2.5601245244568694E-4 86-87 0.0 88-89 0.0 90-91 0.01157176285054505 92-93 0.0022529095815220453 94-95 0.0 96-97 0.0 98-99 7.680373573370608E-4 100-101 0.014234292355980196 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 976515.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.83370239273617 #Duplication Level Percentage of deduplicated Percentage of total 1 78.12994657127349 41.278943451070404 2 12.151493528187112 12.84016785390995 3 3.8517650125272946 6.105090190758624 4 1.731634615405648 3.6595467169320854 5 0.9164545762635192 2.4209844169383956 6 0.5958688094165262 1.8889173205096061 7 0.4122850033013904 1.5247780215789735 8 0.29261538762048783 1.236796344406079 9 0.23866452336388627 1.1348577359200618 >10 1.4934583861568644 15.129777173155793 >50 0.12472337988505618 4.488544957748548 >100 0.057782775104719776 5.409452967168252 >500 0.002334657525158917 0.7978598666482015 >1k 5.836643812897293E-4 0.71398281805442 >5k 3.891095875264862E-4 1.3703001652006153 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7126 0.7297378944511861 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6205 0.635422906970195 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4218 0.43194420976636305 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1689 0.17296201287230611 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1039 0.1063987752364275 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 1.0240498097827478E-4 0.0 0.0 0.0 0.0 16-17 1.0240498097827478E-4 0.0 0.0 0.0 0.0 18-19 1.0240498097827478E-4 0.0 0.0 0.0 0.0 20-21 1.0240498097827478E-4 0.0 0.0 0.0 0.0 22-23 1.0240498097827478E-4 0.0 0.0 1.0240498097827478E-4 0.0 24-25 2.0480996195654957E-4 0.0 0.0 1.0240498097827478E-4 0.0 26-27 2.0480996195654957E-4 0.0 0.0 2.0480996195654957E-4 0.0 28-29 2.0480996195654957E-4 0.0 0.0 5.120249048913739E-4 0.0 30-31 2.0480996195654957E-4 0.0 0.0 0.001433669733695847 0.0 32-33 2.0480996195654957E-4 0.0 0.0 0.002457719543478595 0.0 34-35 2.0480996195654957E-4 0.0 0.0 0.003174554410326518 0.0 36-37 2.0480996195654957E-4 0.0 0.0 0.003942591767663579 0.0 38-39 3.0721494293482434E-4 0.0 0.0 0.0056834764442942505 0.0 40-41 3.0721494293482434E-4 0.0 0.0 0.007782778554348883 0.0 42-43 3.5841743342396174E-4 0.0 0.0 0.011878977793479874 0.0 44-45 4.0961992391309914E-4 0.0 0.0 0.020020173781252718 0.0 46-47 5.120249048913739E-4 0.0 0.0 0.03553452839946135 0.0 48-49 6.144298858696487E-4 0.0 0.0 0.04920559336006103 0.0 50-51 6.144298858696487E-4 0.0 0.0 0.068201717331531 0.0 52-53 6.144298858696487E-4 0.0 0.0 0.09334214016169746 0.0 54-55 6.144298858696487E-4 0.0 0.0 0.11699769076767894 0.0 56-57 6.144298858696487E-4 0.0 0.0 0.1494088672473029 0.0 58-59 6.144298858696487E-4 0.0 0.0 0.19313579412502624 0.0 60-61 6.656323763587861E-4 0.0 0.0 0.24684720664813137 0.0 62-63 7.168348668479235E-4 0.0 0.0 0.31254000194569465 0.0 64-65 7.168348668479235E-4 0.0 0.0 0.37229330834651797 0.0 66-67 7.168348668479235E-4 0.0 0.0 0.45114514369978953 0.0 68-69 8.192398478261983E-4 0.0 0.0 0.5506827852106726 0.0 70-71 8.192398478261983E-4 0.0 0.0 0.6369589816848691 0.0 72-73 8.704423383153357E-4 0.0 0.0 0.7534446475476567 0.0 74-75 9.216448288044731E-4 0.0 0.0 0.8654244942474001 0.0 76-77 9.216448288044731E-4 0.0 0.0 0.983497437315351 0.0 78-79 9.216448288044731E-4 0.0 0.0 1.1267620057039576 0.0 80-81 0.0011264547907610227 0.0 0.0 1.2702313840545205 0.0 82-83 0.0012288597717392974 0.0 0.0 1.44298858696487 0.0 84-85 0.0012288597717392974 0.0 0.0 1.6518947481605506 0.0 86-87 0.0012288597717392974 0.0 0.0 1.8822035503806904 0.0 88-89 0.0012288597717392974 0.0 0.0 2.1672990174242077 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3415 0.0 54.272858 1 GTATCAA 6145 0.0 42.76732 1 ATCAACG 8100 0.0 32.016346 3 TATCAAC 8365 0.0 31.342764 2 TCAACGC 8285 0.0 31.301434 4 CAACGCA 8375 0.0 30.965061 5 AACGCAG 8575 0.0 30.298233 6 GTAAGGT 410 0.0 30.119905 4 GCGACAG 250 0.0 28.503902 7 TGCGCGA 35 0.008395763 27.141014 34-35 TGGTATC 1470 0.0 27.141014 2 ACGCAGA 9640 0.0 26.857931 7 CGCAGAG 9645 0.0 26.844007 8 TAAGGTG 485 0.0 26.441504 5 GCAGAGT 10245 0.0 25.040031 9 GGTAAGG 445 0.0 24.548895 3 GGACCGA 330 0.0 24.468035 6 GAGTACT 6230 0.0 23.829506 12-13 CGCGGAA 110 2.1700544E-9 23.75082 82-83 AGGCGAC 300 0.0 23.748386 5 >>END_MODULE