FastQCFastQC Report
Thu 26 May 2016
SRR937556_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937556_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850235
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59320.6976894623251219No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46930.5519650449581587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30140.35449022917193485No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12610.14831193728792627No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12040.1416079084017948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26150.054.914741
GTATCAA50250.045.799741
ATCAACG64450.035.2516373
TCAACGC65350.034.6288574
TATATCG550.002568852234.573915
TATCAAC66450.034.4767532
CAACGCA66250.034.156415
AACGCAG68800.032.9362146
TGGTATC11200.029.7066922
GTACATG58950.029.3610291
ATCGTAC650.00583992429.2341448
TACATGG58900.028.970332
ACGCAGA78450.028.8242347
CGCAGAG78950.028.5815128
ACATGGG58850.027.6219253
GCAGAGT82700.026.884989
GTGGTAT14000.024.794111
CATGGGG23200.024.5906544
CATGGGA31850.024.330724
GAGTACT48750.023.96705212-13
Produced by FastQC (version 0.11.5)