##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937556_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 850235 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.825308297117857 33.0 31.0 34.0 30.0 34.0 2 31.923188295000795 33.0 31.0 34.0 30.0 34.0 3 31.95417972678142 34.0 31.0 34.0 30.0 34.0 4 35.55940181244009 37.0 35.0 37.0 33.0 37.0 5 35.57821896299259 37.0 35.0 37.0 33.0 37.0 6 35.64120272630508 37.0 35.0 37.0 33.0 37.0 7 35.56601410198357 37.0 35.0 37.0 33.0 37.0 8 35.56674331214311 37.0 35.0 37.0 33.0 37.0 9 37.137950096149886 39.0 37.0 39.0 34.0 39.0 10-11 37.06499673619646 39.0 37.0 39.0 33.0 39.0 12-13 36.96460331555394 39.0 37.0 39.0 33.0 39.0 14-15 38.0814921756926 40.0 38.0 41.0 33.0 41.0 16-17 38.00001470181773 40.0 38.0 41.0 33.0 41.0 18-19 37.97083394590907 40.0 38.0 41.0 33.0 41.0 20-21 37.99526660276277 40.0 38.0 41.0 33.0 41.0 22-23 37.98696772068899 40.0 38.0 41.0 33.0 41.0 24-25 37.91285526942551 40.0 38.0 41.0 33.0 41.0 26-27 37.80378307173899 40.0 38.0 41.0 33.0 41.0 28-29 37.649806818114996 40.0 37.5 41.0 33.0 41.0 30-31 37.36373884867125 40.0 37.0 41.0 32.0 41.0 32-33 37.17479990826066 39.5 36.0 41.0 32.0 41.0 34-35 37.12154051526931 40.0 36.0 41.0 31.5 41.0 36-37 36.943020459049556 40.0 36.0 41.0 31.0 41.0 38-39 36.762765000264636 40.0 36.0 41.0 31.0 41.0 40-41 36.493452398454544 39.0 35.5 41.0 30.0 41.0 42-43 36.18173681393968 39.0 35.0 41.0 30.0 41.0 44-45 35.84287755738119 39.0 35.0 41.0 28.5 41.0 46-47 35.36369415514534 39.0 35.0 40.5 26.5 41.0 48-49 34.918019135885956 39.0 35.0 40.0 24.0 41.0 50-51 34.00268043540903 38.0 34.0 39.5 22.5 40.5 52-53 34.08926355654613 38.0 34.0 39.5 23.0 40.5 54-55 34.326523843408 38.0 34.0 40.0 22.5 41.0 56-57 34.079135768346404 38.0 34.0 40.0 21.0 41.0 58-59 33.799494257469995 38.0 34.0 40.0 19.5 41.0 60-61 33.4556252095009 37.5 33.0 40.0 18.0 41.0 62-63 33.078895834681 37.0 33.0 40.0 14.0 41.0 64-65 32.649780354843074 36.5 32.0 39.5 10.0 41.0 66-67 32.14306397643004 36.0 31.5 39.0 7.5 40.5 68-69 31.687007121560512 35.5 31.0 39.0 6.0 40.0 70-71 31.216560127494162 35.0 31.0 38.0 2.0 40.0 72-73 30.75259193046628 35.0 30.5 37.5 2.0 39.5 74-75 30.2126082788876 35.0 29.5 37.0 2.0 39.0 76-77 29.691274177139263 34.0 29.0 36.0 2.0 39.0 78-79 29.179228095761758 34.0 29.0 36.0 2.0 38.5 80-81 28.65042841096873 34.0 28.5 35.0 2.0 37.0 82-83 28.120471399083783 34.0 27.0 35.0 2.0 37.0 84-85 27.592762001093817 33.5 26.0 35.0 2.0 36.0 86-87 27.02861032538063 33.0 25.5 35.0 2.0 36.0 88-89 26.4892300364017 33.0 24.5 35.0 2.0 35.5 90-91 25.93665398389857 32.0 23.5 35.0 2.0 35.0 92-93 25.28042188336166 32.0 19.5 34.0 2.0 35.0 94-95 24.535389039500842 31.5 14.5 34.0 2.0 35.0 96-97 23.66855222379695 31.0 2.0 34.0 2.0 35.0 98-99 22.504958041012188 31.0 2.0 34.0 2.0 35.0 100-101 19.38888660193946 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.0850875993456306 1101 2 -0.08562932103686194 1101 3 0.004437889362936431 1101 4 -0.1560564903799957 1101 5 -0.141271023919316 1101 6 -0.1397180740406938 1101 7 -0.1153277041712073 1101 8 -0.17973393270314375 1101 9 0.05033635292932814 1101 10-11 0.09694769721669161 1101 12-13 -0.02076961644461761 1101 14-15 0.11146709780967257 1101 16-17 0.11027051241477892 1101 18-19 0.04925700508083963 1101 20-21 -0.03829209342148232 1101 22-23 -0.0770623417993832 1101 24-25 0.04014098240717345 1101 26-27 0.11302662288264287 1101 28-29 -0.011420185529203764 1101 30-31 0.015307264511719154 1101 32-33 0.030689500989197427 1101 34-35 0.15456078344454482 1101 36-37 0.2490454739794643 1101 38-39 0.1337608555473011 1101 40-41 0.2676967883730015 1101 42-43 0.02212758235307888 1101 44-45 0.4861972386691704 1101 46-47 0.536443429259279 1101 48-49 0.5628068241823172 1101 50-51 0.4631582846088378 1101 52-53 0.4527444822675264 1101 54-55 0.3587256076226666 1101 56-57 0.5567439163127474 1101 58-59 0.5509647206358324 1101 60-61 0.7426506772905341 1101 62-63 0.6852508762992997 1101 64-65 0.932085007073475 1101 66-67 1.0496244400137797 1101 68-69 0.7281294837669101 1101 70-71 0.9694336127972143 1101 72-73 1.086154905247561 1101 74-75 1.0639725396682707 1101 76-77 0.8040521989797149 1101 78-79 0.9278444670231778 1101 80-81 1.2707695115719346 1101 82-83 1.4363088206648094 1101 84-85 1.5419129315806543 1101 86-87 1.7220323053243867 1101 88-89 1.8090370656414265 1101 90-91 1.873347213033533 1101 92-93 1.8935575066991177 1101 94-95 2.11705049611615 1101 96-97 2.2663034745484474 1101 98-99 2.318647669534325 1101 100-101 3.0584210644834933 1102 1 -0.04558291891664368 1102 2 0.00836361182532741 1102 3 -0.13124185770791996 1102 4 0.0676370190118547 1102 5 0.055285296209632406 1102 6 0.09166869677326872 1102 7 0.14587742880257082 1102 8 0.06514859162889053 1102 9 0.10202254721362891 1102 10-11 0.17265441099760892 1102 12-13 0.01935498835359084 1102 14-15 0.15517486546202264 1102 16-17 0.24655313593470396 1102 18-19 0.18322421108171483 1102 20-21 0.20593641070100688 1102 22-23 0.10814781256178918 1102 24-25 0.14037965328932245 1102 26-27 0.009892905196203117 1102 28-29 0.20324722504670234 1102 30-31 0.2478951387910584 1102 32-33 0.2738455359529368 1102 34-35 0.008238484764987675 1102 36-37 0.25384833803005336 1102 38-39 0.28793294655219626 1102 40-41 0.2047822139510771 1102 42-43 0.13508400166992374 1102 44-45 0.3013667879841506 1102 46-47 0.33960814316417753 1102 48-49 0.43117828286435866 1102 50-51 0.3601284042542474 1102 52-53 0.47871931333813933 1102 54-55 0.49622173309535356 1102 56-57 0.4102774852173354 1102 58-59 0.4002409589347593 1102 60-61 0.5674073569756146 1102 62-63 0.6804418130050252 1102 64-65 0.669867847566934 1102 66-67 0.7136905239515414 1102 68-69 0.7200689171560377 1102 70-71 0.6867341521314145 1102 72-73 0.698424758325487 1102 74-75 0.7468812248086856 1102 76-77 0.6087543253203016 1102 78-79 0.845391426294146 1102 80-81 0.9314818046721776 1102 82-83 1.055949574492839 1102 84-85 1.140742824333742 1102 86-87 1.1114858419929092 1102 88-89 1.209706583960191 1102 90-91 1.4479985055758462 1102 92-93 1.5861757572725494 1102 94-95 1.4004380728317898 1102 96-97 1.4076043858372707 1102 98-99 1.5231777668624495 1102 100-101 2.092076758488826 1103 1 0.18408500058922073 1103 2 0.09049799991804619 1103 3 0.20279053490251542 1103 4 0.15483978213151772 1103 5 0.160178554934987 1103 6 0.06578241458139189 1103 7 -0.045350041180839185 1103 8 0.12495916609158542 1103 9 0.25077611280647716 1103 10-11 0.28558607033782124 1103 12-13 0.18334062600500545 1103 14-15 0.350336984728564 1103 16-17 0.32545037187775705 1103 18-19 0.41236022067952405 1103 20-21 0.26002797731238303 1103 22-23 0.16966854376997986 1103 24-25 -0.0032684363652251136 1103 26-27 0.16489153050980576 1103 28-29 0.07092194300167165 1103 30-31 -0.04999940401636138 1103 32-33 0.07481789225915492 1103 34-35 0.21034862838847346 1103 36-37 0.1991373814670112 1103 38-39 0.2618228872844668 1103 40-41 0.2543818365371777 1103 42-43 0.316535186243442 1103 44-45 0.25232758442746217 1103 46-47 0.06425533302399344 1103 48-49 0.12856372306081454 1103 50-51 0.010663985199840909 1103 52-53 0.015811632902739348 1103 54-55 0.008793084005922935 1103 56-57 0.05871120190071366 1103 58-59 0.13064269541362705 1103 60-61 0.1075513241331052 1103 62-63 0.34058965427873744 1103 64-65 0.24961723286340742 1103 66-67 0.37571918616296784 1103 68-69 0.44578851862194924 1103 70-71 0.7453352368839568 1103 72-73 0.5302646823428177 1103 74-75 0.4863281634758003 1103 76-77 0.6597446462783338 1103 78-79 0.7338754596240413 1103 80-81 0.7187510370035497 1103 82-83 0.9661938781360746 1103 84-85 1.0713535756311003 1103 86-87 1.289588962218609 1103 88-89 1.2468156965688735 1103 90-91 1.3062105384558436 1103 92-93 1.3104335828310951 1103 94-95 1.2498591424002932 1103 96-97 1.4476776659952328 1103 98-99 1.9906472972508347 1103 100-101 2.507347259280831 1104 1 0.11889495061215172 1104 2 -0.05407851889782478 1104 3 -0.04398128692001535 1104 4 0.04469873994179352 1104 5 0.08589381330744317 1104 6 0.06513228715641617 1104 7 0.09348644349743296 1104 8 0.07892541620388016 1104 9 0.1326846032342388 1104 10-11 0.19783896281384017 1104 12-13 0.1447360595099738 1104 14-15 0.24544542598304275 1104 16-17 0.2196616372857747 1104 18-19 0.1248715092557191 1104 20-21 0.047536783583858266 1104 22-23 0.07365536154990338 1104 24-25 0.16831201158439768 1104 26-27 0.15074357569247354 1104 28-29 0.12740146753393589 1104 30-31 0.15414533562532995 1104 32-33 0.29523354827318826 1104 34-35 0.3080296829660796 1104 36-37 0.09851775092852222 1104 38-39 0.23685917621528318 1104 40-41 0.29833990686441325 1104 42-43 0.25202954313738957 1104 44-45 0.5975937111337473 1104 46-47 0.3870766969204098 1104 48-49 0.511774832438256 1104 50-51 0.29868225496253586 1104 52-53 0.20734153640018604 1104 54-55 0.20449444147198648 1104 56-57 0.36507724964607036 1104 58-59 0.5052709407315206 1104 60-61 0.3691259563973901 1104 62-63 0.4346950080189771 1104 64-65 0.661815751045701 1104 66-67 0.7921612633555597 1104 68-69 0.740468821415849 1104 70-71 0.6789607729435687 1104 72-73 0.5670607125465565 1104 74-75 0.6026253213551094 1104 76-77 0.7889687014658797 1104 78-79 1.0390228139600381 1104 80-81 1.162332979064196 1104 82-83 1.2104621183475182 1104 84-85 1.4087531604951877 1104 86-87 1.7196376153909938 1104 88-89 1.506234269881972 1104 90-91 1.6109914573341264 1104 92-93 1.6774021450329215 1104 94-95 1.913764540585614 1104 96-97 1.5107494826167454 1104 98-99 1.8008786480501335 1104 100-101 2.4879852825152042 1105 1 0.14980706975956437 1105 2 0.255297319889781 1105 3 0.11769738946454211 1105 4 0.16525015461085957 1105 5 0.07062357744896985 1105 6 0.13932412605414157 1105 7 0.104647910541253 1105 8 0.11726532219084618 1105 9 0.2989985489599505 1105 10-11 0.3089168091858099 1105 12-13 0.31849069649990014 1105 14-15 0.3191924923771765 1105 16-17 0.3231422187075381 1105 18-19 0.3373471629078395 1105 20-21 0.23493827980888682 1105 22-23 0.22269888559342377 1105 24-25 0.3307899020034668 1105 26-27 0.3975673666339219 1105 28-29 0.38244623728459004 1105 30-31 0.29789718525953646 1105 32-33 0.3335700130802408 1105 34-35 0.19386681880321532 1105 36-37 0.4200676239489809 1105 38-39 0.4651281250724608 1105 40-41 0.5829951694608582 1105 42-43 0.4369602721857646 1105 44-45 0.601095971988947 1105 46-47 0.7713584194374263 1105 48-49 0.7760832585113846 1105 50-51 0.6796759300738557 1105 52-53 0.6243136077840248 1105 54-55 0.671363621576468 1105 56-57 0.7209331055019348 1105 58-59 0.6788113642719509 1105 60-61 0.9256808879523177 1105 62-63 1.0131754173817384 1105 64-65 0.9390466679825025 1105 66-67 1.079447307994542 1105 68-69 0.8612189642248076 1105 70-71 0.6719381850386306 1105 72-73 0.8908490730996235 1105 74-75 0.9430113529176118 1105 76-77 0.9025044105898239 1105 78-79 1.1610694213007626 1105 80-81 1.3007329336406208 1105 82-83 1.262209721565707 1105 84-85 1.3487475857837303 1105 86-87 1.4384398783109056 1105 88-89 1.4366940459299862 1105 90-91 1.4540117121190868 1105 92-93 1.557717907701626 1105 94-95 1.7177566534012563 1105 96-97 1.6209214383769357 1105 98-99 1.9285558860214884 1105 100-101 2.5051171967059425 1106 1 -0.30050544122028455 1106 2 -0.16975470020774353 1106 3 -0.17465848656170735 1106 4 -0.21793983879147305 1106 5 -0.27059800669475464 1106 6 -0.255819753922907 1106 7 -0.13803804422761345 1106 8 -0.09628352204556023 1106 9 -0.21106094719661428 1106 10-11 -0.39380053331920095 1106 12-13 -0.4741848152099877 1106 14-15 -0.7157502757556884 1106 16-17 -0.5281822930062603 1106 18-19 -0.09295957023002899 1106 20-21 -0.0823433236203428 1106 22-23 -0.18666268238795425 1106 24-25 -0.39413650859028593 1106 26-27 -0.3683753780752568 1106 28-29 -0.1287698032540021 1106 30-31 -0.07857411487202626 1106 32-33 0.03589012894939714 1106 34-35 -0.025755591415759227 1106 36-37 -0.3523873928184713 1106 38-39 -0.4333594857508416 1106 40-41 -0.3418348136350531 1106 42-43 -0.11856166604597718 1106 44-45 -0.2661332209480278 1106 46-47 0.08820668471845039 1106 48-49 -0.056951748183884376 1106 50-51 0.020968761255460322 1106 52-53 0.0652229737439427 1106 54-55 0.16022809995055098 1106 56-57 0.014554836434079732 1106 58-59 -0.38533634547738416 1106 60-61 0.09177969295405575 1106 62-63 -0.04433997393041267 1106 64-65 -9.033029330893783E-4 1106 66-67 -0.028592969029560322 1106 68-69 -0.23057991612310857 1106 70-71 -0.10828178287092527 1106 72-73 -0.20403587757400388 1106 74-75 -0.21674695370621677 1106 76-77 0.2410269031820853 1106 78-79 0.18481322866438887 1106 80-81 -0.03574291458952672 1106 82-83 -0.07697058614341401 1106 84-85 0.08307714139741051 1106 86-87 0.31841399514042834 1106 88-89 0.43849132565951976 1106 90-91 0.8243450311379483 1106 92-93 0.7377337967670599 1106 94-95 0.6172407467680578 1106 96-97 0.7060592086764856 1106 98-99 1.0234301008400948 1106 100-101 1.7472273192508467 1107 1 0.04774078792904035 1107 2 0.17134513868808554 1107 3 0.12429105863188639 1107 4 0.21930800139954698 1107 5 0.20686429473094847 1107 6 0.31404403260116 1107 7 0.1687432295481628 1107 8 0.1698800334499566 1107 9 0.1996573469664824 1107 10-11 0.26669229446620335 1107 12-13 0.2126990010716625 1107 14-15 0.2033405683228935 1107 16-17 0.2696731939713217 1107 18-19 0.2440515799265981 1107 20-21 0.36481858110372656 1107 22-23 0.41867232886549033 1107 24-25 0.2704162038596323 1107 26-27 0.43876594663407076 1107 28-29 0.31549878183025015 1107 30-31 0.2869570421975851 1107 32-33 0.32008304870919346 1107 34-35 0.3514302902173654 1107 36-37 0.376537460330411 1107 38-39 0.4927714714780862 1107 40-41 0.4735226250668987 1107 42-43 0.5473895338920443 1107 44-45 0.45478459606068355 1107 46-47 0.6850509500258326 1107 48-49 0.7692866780409346 1107 50-51 0.40595806401807266 1107 52-53 0.6437106009040008 1107 54-55 0.5673165840814889 1107 56-57 0.5823333072754124 1107 58-59 0.5752090249132209 1107 60-61 0.95878482405233 1107 62-63 0.9088325465850389 1107 64-65 0.6850026646752383 1107 66-67 0.7927706158659831 1107 68-69 0.8761898360227924 1107 70-71 1.100950519011672 1107 72-73 0.8054471190827357 1107 74-75 0.7182482091957247 1107 76-77 0.8488191999792676 1107 78-79 1.1403819502203625 1107 80-81 1.143437798202065 1107 82-83 1.3387973602849783 1107 84-85 1.5522031463119816 1107 86-87 1.4160513657455738 1107 88-89 1.3193591626324306 1107 90-91 1.7109751729135922 1107 92-93 1.559121219517511 1107 94-95 1.7528508270023018 1107 96-97 1.7163551755100848 1107 98-99 1.9924473455416312 1107 100-101 2.3754625631220385 1108 1 -0.09580336008832546 1108 2 -0.2111155032272407 1108 3 0.036156900298671246 1108 4 -0.07660004476237248 1108 5 -0.06619605399706785 1108 6 0.0415205906866305 1108 7 -0.09581307830254104 1108 8 -0.009474994672295622 1108 9 0.011176913901870478 1108 10-11 -0.018251893366731053 1108 12-13 -0.010837224051151395 1108 14-15 -0.0869998346186307 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0.048577698747834575 2316 52-53 -0.440803453631446 2316 54-55 -0.48735200097743103 2316 56-57 -0.6664337602248409 2316 58-59 -0.6522323380701636 2316 60-61 -0.5985174480341655 2316 62-63 -0.820342977355331 2316 64-65 -0.7339762166007482 2316 66-67 -0.8653278039218009 2316 68-69 -0.862649999108843 2316 70-71 -0.9999765298715317 2316 72-73 -0.7776773690600756 2316 74-75 -0.6610004851489002 2316 76-77 -0.42690232286019025 2316 78-79 -0.804537741345122 2316 80-81 -0.7502021944585167 2316 82-83 -0.9189683243617672 2316 84-85 -1.1636159818190173 2316 86-87 -1.3732570284885064 2316 88-89 -1.619469708126708 2316 90-91 -1.4711318662135575 2316 92-93 -1.3318850861154914 2316 94-95 -1.5100535681447305 2316 96-97 -1.5849793452573344 2316 98-99 -1.9388474349695315 2316 100-101 -2.370431391257224 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3579.0 3 749.0 4 689.0 5 780.0 6 901.0 7 1030.0 8 1209.0 9 1564.0 10 1990.0 11 2519.0 12 3588.0 13 4809.0 14 5367.0 15 7190.0 16 9380.0 17 12570.0 18 10761.0 19 6857.0 20 6361.0 21 6593.0 22 6864.0 23 7650.0 24 8351.0 25 9466.0 26 11050.0 27 12847.0 28 15075.0 29 17972.0 30 22081.0 31 27272.0 32 34707.0 33 45280.0 34 61458.0 35 86359.0 36 122223.0 37 157585.0 38 105739.0 39 9769.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.25200678802984 19.123433100378346 14.957267983204584 21.66729212838723 2 15.125884897822461 20.088625421365876 41.52196528110323 23.263524399708434 3 19.031925509503576 25.650930777342396 30.12021901753509 25.196924695618943 4 12.229143248735873 16.76159652001194 37.54681128873026 33.46244894252193 5 12.95961237695115 38.16183356647645 33.88859139878797 14.989962657784424 6 30.775713613855842 36.544030891343986 17.79988755211323 14.88036794268694 7 28.197029286385234 34.04970989401084 20.210422601277813 17.542838218326118 8 26.760638084708642 34.544338023653545 19.539399396992508 19.155624494645306 9 27.2489507077198 16.760624636828776 20.586283590023445 35.404141065427986 10-11 24.81614981947969 27.275422245429827 26.62789477840734 21.28053315668314 12-13 25.976745529278496 24.911387123049014 27.724249221727277 21.387618125945213 14-15 22.967554181464145 25.693747870989945 26.773546409177758 24.56515153836815 16-17 21.30682487825345 29.306916806916806 27.420611349182778 21.965646965646965 18-19 21.231052436813957 26.746558149072623 31.255553748500343 20.766835665613076 20-21 21.969579566821423 25.755723202695446 31.57152596543599 20.703171265047143 22-23 21.795307718463107 25.774724993031644 31.437213789153173 20.992753499352077 24-25 21.278628147144182 26.071568526348354 31.656152860236595 20.993650466270864 26-27 20.910679399952265 26.367212477126937 31.40456076352568 21.31754735939512 28-29 20.887206515288405 26.483063208646346 31.557706259219508 21.072024016845738 30-31 20.92963223372587 26.223849378549787 31.529794779099184 21.31672360862516 32-33 20.78348471597191 26.259367611108885 31.459496795151352 21.497650877767853 34-35 21.3988134001972 26.38342838586712 31.217699868162125 21.000058345773557 36-37 21.37004770794441 25.987335237879734 31.148290622466106 21.494326431709755 38-39 20.984529230421135 26.47707667505727 31.065218108625352 21.47317598589624 40-41 21.279535434272447 26.35942685805964 31.15792041318823 21.203117294479686 42-43 21.092381812139223 26.20969167564306 31.045158522701826 21.652767989515887 44-45 21.211312322780916 26.555108505808896 30.761225937684877 21.472353233725308 46-47 21.46367680024892 26.996603281687186 29.95729940857972 21.582420509484173 48-49 21.524473397916815 27.083866192211087 29.647010705896083 21.74464970397602 50-51 21.756433504492712 27.41824747960791 29.276091885065288 21.54922713083409 52-53 21.819983237477324 27.329984758128113 29.25148617091879 21.59854583347577 54-55 21.498511083046385 27.349751395362404 29.357093143792312 21.794644377798903 56-57 21.64090950064425 27.79452633464265 29.021396584158506 21.543167580554602 58-59 21.653032180842292 27.17529346345785 29.182590583722494 21.98908377197736 60-61 21.50935348098432 27.356457413264078 29.567451582951165 21.566737522800437 62-63 21.438611663571837 27.297791518601954 29.668751354968848 21.59484546285736 64-65 21.322858805979294 27.227624189606697 29.597059842434327 21.85245716197968 66-67 21.417432038568972 27.513561060564196 29.099175584140436 21.9698313167264 68-69 21.48523111293356 27.305885873996672 29.402033284787127 21.806849728282643 70-71 21.420441021556183 27.477770995892374 29.298039511421454 21.803748471129985 72-73 21.43346198037887 27.44524644256548 29.106266387767725 22.015025189287922 74-75 21.32332707352845 27.972430145821832 28.837508860854967 21.866733919794754 76-77 21.48083503581848 27.609958344848557 29.001213296885318 21.90799332244764 78-79 21.509559372050077 27.682876096443415 28.85341746224982 21.95414706925669 80-81 21.394774955896235 28.01676237704271 28.722010165372918 21.86645250168814 82-83 21.593349456933197 27.970812552841124 28.517915165512385 21.917922824713294 84-85 21.481795687522094 27.919523977848478 28.468716861081656 22.12996347354778 86-87 21.496763687310803 27.809900654096914 28.55708648195784 22.136249176634447 88-89 21.571096417161517 27.74245704258797 28.46136985268209 22.225076687568425 90-91 21.471852238540393 27.76472376624861 28.728003761440757 22.035420233770243 92-93 21.550403582159902 27.445472220585636 28.765392093324692 22.23873210392977 94-95 21.373580307867794 27.43154222002525 28.777454844112988 22.417422627993968 96-97 21.59565297576364 27.33164384522152 28.590334093229814 22.482369085785024 98-99 21.31074708671476 27.52225952028679 28.384239593810523 22.782753799187926 100-101 21.701330489927805 26.732332053997215 29.096285997896903 22.47005145817808 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1205.0 1 717.0 2 294.0 3 332.5 4 260.0 5 253.0 6 434.0 7 922.0 8 1566.5 9 1898.0 10 1703.0 11 1495.0 12 1588.0 13 1713.5 14 1779.5 15 1859.0 16 1949.5 17 1958.5 18 2007.5 19 2148.0 20 2332.5 21 2638.0 22 3087.5 23 3678.5 24 4357.0 25 5237.5 26 6565.5 27 7852.5 28 8667.0 29 9684.0 30 11055.5 31 12893.0 32 15264.0 33 16889.0 34 18546.0 35 20257.0 36 22489.0 37 24965.5 38 25780.0 39 25720.5 40 25759.0 41 26908.5 42 27793.0 43 28324.5 44 29307.5 45 30539.0 46 30699.0 47 30317.5 48 30628.5 49 30243.0 50 29608.5 51 28166.5 52 26188.0 53 25272.5 54 24920.5 55 23153.5 56 20506.5 57 18320.0 58 16814.5 59 15033.0 60 12313.5 61 10265.0 62 8771.0 63 6648.5 64 4923.0 65 3969.0 66 2890.0 67 2115.0 68 1616.0 69 1196.5 70 874.5 71 612.0 72 489.5 73 398.5 74 311.5 75 255.5 76 192.5 77 139.5 78 92.5 79 67.5 80 48.0 81 28.5 82 17.0 83 14.5 84 10.0 85 5.5 86 4.0 87 4.0 88 4.0 89 2.0 90 2.5 91 3.5 92 3.0 93 1.5 94 0.5 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3364952042670556 2 0.28192205684310806 3 0.2880380130199298 4 0.2841567331384852 5 0.2823925150105559 6 0.2166459861097226 7 0.2060606773421466 8 0.21417608073062153 9 0.2124118626026922 10-11 0.2180573606120661 12-13 0.22135056778420084 14-15 0.21594029885855084 16-17 0.20594306280028465 18-19 0.20100325204208247 20-21 0.19918022664322219 22-23 0.2065899427805254 24-25 0.20553141190376775 26-27 0.21200021170617536 28-29 0.2152934188783101 30-31 0.21835139696672096 32-33 0.21558745523296502 34-35 0.21694002246437752 36-37 0.20453168829794116 38-39 0.1960634412838803 40-41 0.18988867783612765 42-43 0.2014737102095303 44-45 0.20100325204208247 46-47 0.20806012455379985 48-49 0.2145289243562074 50-51 0.2123530553317612 52-53 0.22511423312378342 54-55 0.21164736808058948 56-57 0.18483125253606356 58-59 0.16548366039977183 60-61 0.18430198709768475 62-63 0.17724511458596742 64-65 0.1850076743488565 66-67 0.18506648161978745 68-69 0.1833610707627891 70-71 0.18453721618140867 72-73 0.19524013949084665 74-75 0.19988591389439392 76-77 0.202355819273495 78-79 0.19947426299787704 80-81 0.1961810558257423 82-83 0.1861250124965451 84-85 0.1805383217581022 86-87 0.18630143430933802 88-89 0.19547536857457054 90-91 0.19100601598381622 92-93 0.18641904885119998 94-95 0.18718354337330267 96-97 0.19459325951060588 98-99 0.1958870194710874 100-101 0.17495163101965927 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 850235.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.158657520023034 #Duplication Level Percentage of deduplicated Percentage of total 1 80.22874738413638 45.05538747606159 2 11.109712417141923 12.478130695604412 3 3.4323437222865474 5.782674467726737 4 1.4914215217806444 3.350249218386831 5 0.8334122972850985 2.3401657888104737 6 0.5161760412672043 1.7392652112939722 7 0.3733916138956459 1.4678420235929976 8 0.2780591659328523 1.2492343575941045 9 0.20414976445037478 1.0318299034105773 >10 1.380690100541131 14.791541726946644 >50 0.10504666256255493 4.0184475888893125 >100 0.044538356277295264 4.3770070328196296 >500 0.0012605195140091134 0.4182455027695122 >1k 8.403463426727422E-4 1.200110932065297 >5k 2.1008658566818556E-4 0.6998680740278551 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5932 0.6976894623251219 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4693 0.5519650449581587 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3014 0.35449022917193485 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1261 0.14831193728792627 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1204 0.1416079084017948 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 1.176145418619558E-4 0.0 0.0 0.0 0.0 20-21 2.352290837239116E-4 0.0 0.0 0.0 0.0 22-23 2.352290837239116E-4 0.0 0.0 0.0 0.0 24-25 5.88072709309779E-4 0.0 0.0 0.0 0.0 26-27 5.88072709309779E-4 0.0 0.0 1.176145418619558E-4 0.0 28-29 8.233017930336906E-4 0.0 0.0 8.821090639646686E-4 0.0 30-31 9.409163348956465E-4 0.0 0.0 0.001293759960481514 0.0 32-33 9.409163348956465E-4 0.0 0.0 0.0017642181279293372 0.0 34-35 9.997236058266244E-4 0.0 0.0 0.0021758690244461825 0.0 36-37 0.0010585308767576023 0.0 0.0 0.003234399901203785 0.0 38-39 0.0010585308767576023 0.0 0.0 0.004586967132616276 0.0 40-41 0.0010585308767576023 0.0 0.0 0.006527607073338547 0.0 42-43 0.0010585308767576023 0.0 0.0 0.00952677789081842 0.0 44-45 0.0010585308767576023 0.0 0.0 0.017583374008362393 0.0 46-47 0.0011173381476885802 0.0 0.0 0.03252042082483078 0.0 48-49 0.001176145418619558 0.0 0.0 0.04610490040988668 0.0 50-51 0.001176145418619558 0.0 0.0 0.06310020170893929 0.0 52-53 0.001176145418619558 0.0 0.0 0.08450604832781525 0.0 54-55 0.001176145418619558 0.0 0.0 0.10802895670020642 0.0 56-57 0.0014113745023434697 0.0 0.0 0.1360212176633519 0.0 58-59 0.0014113745023434697 0.0 0.0 0.17753915094062228 0.0 60-61 0.0014113745023434697 0.0 0.0 0.22952477844360675 0.0 62-63 0.0014113745023434697 0.0 0.0 0.29556534369909493 0.0 64-65 0.0014113745023434697 0.0 0.0 0.35372573464983215 0.0 66-67 0.0014113745023434697 0.0 0.0 0.4378201320811305 0.0 68-69 0.0014113745023434697 0.0 0.0 0.537674878121931 0.0 70-71 0.0014113745023434697 0.0 0.0 0.6355889842220093 0.0 72-73 0.0014113745023434697 0.0 0.0 0.7636712203096792 0.0 74-75 0.0014113745023434697 0.0 0.0 0.8845201620728387 0.0 76-77 0.0014113745023434697 0.0 0.0 1.0073685510476516 0.0 78-79 0.0014113745023434697 0.0 0.0 1.1547395720006821 0.0 80-81 0.0014113745023434697 0.0 0.0 1.3026986656630224 0.0 82-83 0.0014113745023434697 0.0 0.0 1.4890000999723605 0.0 84-85 0.0014701817732744476 0.0 0.0 1.7077043405646672 0.0 86-87 0.0015289890442054255 0.0 0.0 1.9553417584550155 0.0 88-89 0.0015289890442054255 0.0 0.0 2.265256076261269 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2615 0.0 54.91474 1 GTATCAA 5025 0.0 45.79974 1 ATCAACG 6445 0.0 35.251637 3 TCAACGC 6535 0.0 34.628857 4 TATATCG 55 0.0025688522 34.57391 5 TATCAAC 6645 0.0 34.476753 2 CAACGCA 6625 0.0 34.15641 5 AACGCAG 6880 0.0 32.936214 6 TGGTATC 1120 0.0 29.706692 2 GTACATG 5895 0.0 29.361029 1 ATCGTAC 65 0.005839924 29.234144 8 TACATGG 5890 0.0 28.97033 2 ACGCAGA 7845 0.0 28.824234 7 CGCAGAG 7895 0.0 28.581512 8 ACATGGG 5885 0.0 27.621925 3 GCAGAGT 8270 0.0 26.88498 9 GTGGTAT 1400 0.0 24.79411 1 CATGGGG 2320 0.0 24.590654 4 CATGGGA 3185 0.0 24.33072 4 GAGTACT 4875 0.0 23.967052 12-13 >>END_MODULE