FastQCFastQC Report
Thu 26 May 2016
SRR937556_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937556_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850235
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62920.740030697395426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53810.6328838497591842No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35730.42023675807276817No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13760.1618376096020512No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9500.11173381476885802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28050.055.0720941
GTATCAA53050.043.902761
ATCAACG69350.033.5680663
TCAACGC71200.032.695864
TATCAAC71250.032.6729162
CAACGCA72350.032.1761635
AACGCAG73700.031.5793466
TAAGGTG4250.027.9465225
ACGCAGA84600.027.4560837
CGCAGAG84950.027.2870458
CCCTATA1457.870367E-726.211912
GCAGAGT90450.025.2076729
TGGTATC12500.024.3246522
GTACATG55500.024.1511171
TACATGG55800.023.6694032
GAGTACT55350.023.38562412-13
CCTATAC2052.6617272E-823.1751653
GTAAGGT3900.023.1454544
ACATGGG55250.023.131123
ACTGTCG1050.002195564822.6193858
Produced by FastQC (version 0.11.5)