##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937556_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 850235 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26937728980811 33.0 31.0 34.0 31.0 34.0 2 32.5018447840891 34.0 31.0 34.0 31.0 34.0 3 32.58360100442819 34.0 31.0 34.0 31.0 34.0 4 36.19451563391298 37.0 35.0 37.0 35.0 37.0 5 36.04131093168359 37.0 35.0 37.0 35.0 37.0 6 36.153334078225434 37.0 35.0 37.0 35.0 37.0 7 36.114258998982635 37.0 35.0 37.0 35.0 37.0 8 36.11170205884255 37.0 35.0 37.0 35.0 37.0 9 37.78990514387199 39.0 38.0 39.0 35.0 39.0 10-11 37.73881926761425 39.0 37.0 39.0 35.0 39.0 12-13 37.658429728251605 39.0 37.0 39.0 35.0 39.0 14-15 38.87109387404659 40.0 38.0 41.0 35.0 41.0 16-17 38.85005851323457 40.0 38.0 41.0 35.0 41.0 18-19 38.869353766899735 40.0 38.0 41.0 35.0 41.0 20-21 38.80782960005175 40.0 38.0 41.0 35.0 41.0 22-23 38.665291948696535 40.0 38.0 41.0 34.5 41.0 24-25 38.59093250689516 40.0 38.0 41.0 34.0 41.0 26-27 38.50597952330826 40.0 38.0 41.0 34.0 41.0 28-29 38.385995636500496 40.0 38.0 41.0 34.0 41.0 30-31 38.403156774303575 40.0 38.0 41.0 34.0 41.0 32-33 38.282747122854275 40.0 38.0 41.0 34.0 41.0 34-35 38.128754991267115 40.0 37.0 41.0 34.0 41.0 36-37 37.948470716919445 40.0 37.0 41.0 33.5 41.0 38-39 37.790091562920836 40.0 37.0 41.0 33.0 41.0 40-41 37.58035013849112 40.0 37.0 41.0 33.0 41.0 42-43 37.31761924644363 40.0 36.5 41.0 33.0 41.0 44-45 36.94265820626062 40.0 36.0 41.0 31.5 41.0 46-47 36.46792063370715 40.0 36.0 41.0 30.5 41.0 48-49 36.046675330937916 39.0 35.5 41.0 29.5 41.0 50-51 35.75269484319041 39.0 35.0 41.0 28.5 41.0 52-53 35.511380383070566 39.0 35.0 40.5 28.0 41.0 54-55 35.28517292278018 39.0 35.0 40.0 27.0 41.0 56-57 34.99844984033826 38.0 35.0 40.0 26.0 41.0 58-59 34.730181655659905 38.0 34.5 40.0 26.0 41.0 60-61 34.388960699100835 38.0 34.0 40.0 25.5 41.0 62-63 34.02827394779091 37.0 34.0 40.0 24.5 41.0 64-65 33.635216734197016 37.0 33.5 39.5 23.0 41.0 66-67 33.21306873981899 36.0 33.0 39.0 22.0 40.5 68-69 32.748685363458335 36.0 32.5 39.0 21.5 40.0 70-71 32.34147735626033 35.0 32.0 38.0 20.0 40.0 72-73 31.903727792904313 35.0 31.5 37.5 18.5 39.5 74-75 31.41412609455033 35.0 31.0 37.0 17.5 39.0 76-77 30.211003428463897 34.0 29.5 35.5 14.0 38.0 78-79 30.75948825912836 34.0 31.0 36.0 13.5 38.5 80-81 30.55789752244968 34.0 31.0 36.0 9.5 37.5 82-83 30.206126541485588 34.0 31.0 35.0 7.0 37.0 84-85 29.816319605756057 34.0 30.0 35.0 3.5 36.5 86-87 29.407106270619302 34.0 30.0 35.0 2.0 36.0 88-89 28.97925397096097 34.0 29.0 35.0 2.0 36.0 90-91 28.473711385675724 34.0 29.0 35.0 2.0 35.0 92-93 28.02221679888501 33.5 28.0 35.0 2.0 35.0 94-95 27.559502372873382 33.0 27.0 35.0 2.0 35.0 96-97 27.034059407105097 33.0 26.5 35.0 2.0 35.0 98-99 26.23833116726552 33.0 25.0 34.5 2.0 35.0 100-101 23.86192170399948 30.5 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17378887408374055 1101 2 0.13184977959963362 1101 3 0.09598236963276463 1101 4 0.0667659925114279 1101 5 -0.09349081067329479 1101 6 -0.0017402575550349297 1101 7 0.033220643038760045 1101 8 0.1615228923774623 1101 9 0.16051774993619006 1101 10-11 0.11245371737215493 1101 12-13 0.12233800435284081 1101 14-15 0.15464536322903655 1101 16-17 0.05134604964543854 1101 18-19 0.13470480836122078 1101 20-21 0.1737164025316673 1101 22-23 0.19964057847301575 1101 24-25 0.12461302844014455 1101 26-27 0.24054822857035418 1101 28-29 0.11574406997614517 1101 30-31 0.109812795927688 1101 32-33 0.18928281858406848 1101 34-35 0.10177644692987542 1101 36-37 0.20140527842905698 1101 38-39 0.17993005034913523 1101 40-41 0.06239913953177023 1101 42-43 -0.031509646130444935 1101 44-45 -0.028666101858796367 1101 46-47 -0.18115196250534638 1101 48-49 -0.23735442536638374 1101 50-51 -0.2210832156415421 1101 52-53 -0.251376003534304 1101 54-55 -0.14177256846917885 1101 56-57 -0.027801011788611163 1101 58-59 -0.02997670718923473 1101 60-61 -0.24958039559616907 1101 62-63 -0.12342413243246142 1101 64-65 -0.3017635653764472 1101 66-67 -0.24189000394277826 1101 68-69 -0.06711276517896891 1101 70-71 -0.06939975261698805 1101 72-73 -0.16380867748152994 1101 74-75 -0.1718948165747598 1101 76-77 -0.08910979695650312 1101 78-79 -0.08993656372879855 1101 80-81 -0.04530970657539157 1101 82-83 -0.19176574444284356 1101 84-85 0.012935520471952344 1101 86-87 0.16580715374275812 1101 88-89 0.2869987387690145 1101 90-91 0.10709840758055122 1101 92-93 0.30072279508103605 1101 94-95 0.25891993032200133 1101 96-97 -0.00460614157663386 1101 98-99 0.3157428063950327 1101 100-101 0.9515272601142755 1102 1 0.10972588976783015 1102 2 0.15989825768530608 1102 3 0.10508595899486295 1102 4 0.0022907584899769518 1102 5 0.09330480026216748 1102 6 0.0796279155278512 1102 7 0.09157102429225006 1102 8 0.1157166807352823 1102 9 0.10535068031868633 1102 10-11 0.04552203303764202 1102 12-13 0.14056843024088295 1102 14-15 0.020941625085086457 1102 16-17 0.1579218973920149 1102 18-19 0.26399468499626266 1102 20-21 0.16736837699078677 1102 22-23 0.24415889723811546 1102 24-25 0.23699446790453038 1102 26-27 0.05642914109902364 1102 28-29 0.09861091022163038 1102 30-31 0.18450283374307475 1102 32-33 0.2517711950021493 1102 34-35 0.3767578491987962 1102 36-37 0.34193903331095754 1102 38-39 0.2793783176605231 1102 40-41 0.41802960261528455 1102 42-43 0.5406046059308096 1102 44-45 0.4318710626071365 1102 46-47 0.2861191304213122 1102 48-49 0.42780582508306253 1102 50-51 0.45135339709170097 1102 52-53 0.41220188996268803 1102 54-55 0.37838117277439665 1102 56-57 0.5410586373341957 1102 58-59 0.5698497139873879 1102 60-61 0.5364899038273023 1102 62-63 0.6171731646972916 1102 64-65 0.4894815527191483 1102 66-67 0.5746278808752763 1102 68-69 0.45938900160548 1102 70-71 0.6011987843615003 1102 72-73 0.5776394658449782 1102 74-75 0.8276061443080174 1102 76-77 0.6971046348828125 1102 78-79 0.738355234671797 1102 80-81 0.7269967220403188 1102 82-83 0.6551563310639459 1102 84-85 0.7502813142851075 1102 86-87 0.9616087159521669 1102 88-89 1.0803670978292104 1102 90-91 1.1545024742844099 1102 92-93 1.2891379584311053 1102 94-95 0.9129525383438235 1102 96-97 1.1006136216122577 1102 98-99 1.5437230928000893 1102 100-101 2.1939765812970933 1103 1 0.21703890898531597 1103 2 0.2073093170373923 1103 3 0.19675020433889756 1103 4 0.1147463545571199 1103 5 0.03682237281593359 1103 6 0.05860224325933672 1103 7 0.2111023499585798 1103 8 0.15216461605003673 1103 9 0.26175093900967283 1103 10-11 0.14258192250036217 1103 12-13 0.1885660745282749 1103 14-15 0.26929239194139853 1103 16-17 0.23130789058731693 1103 18-19 0.2030851424856479 1103 20-21 0.15850047810522483 1103 22-23 0.22285594447421886 1103 24-25 0.1325089286681731 1103 26-27 0.1418186459932187 1103 28-29 0.13530058726406224 1103 30-31 0.16910126910001821 1103 32-33 0.27655421325110296 1103 34-35 0.28082270315398716 1103 36-37 0.27587012090660323 1103 38-39 0.2683671577063862 1103 40-41 0.202724422234553 1103 42-43 0.1103117508633602 1103 44-45 0.10986756652967955 1103 46-47 0.23856044849562608 1103 48-49 0.3459405162316429 1103 50-51 0.31265730276322046 1103 52-53 0.11898557680912347 1103 54-55 0.18750752832453088 1103 56-57 0.3514034649204021 1103 58-59 0.2782716989720626 1103 60-61 0.2508046851428105 1103 62-63 0.4041737083232775 1103 64-65 0.35602879350484784 1103 66-67 0.4103739450543813 1103 68-69 0.3582851128051132 1103 70-71 0.2854364426457039 1103 72-73 0.30081042996878793 1103 74-75 0.23492611506468464 1103 76-77 0.20536993686079796 1103 78-79 0.35150207929786603 1103 80-81 0.3633726425334558 1103 82-83 0.20627162351285833 1103 84-85 0.45585980731638287 1103 86-87 0.5021385325877468 1103 88-89 0.768689371185598 1103 90-91 0.8229972395410883 1103 92-93 0.9330695771555853 1103 94-95 1.0437656653492269 1103 96-97 1.0370581983181957 1103 98-99 1.3998125630146987 1103 100-101 1.9787237327281701 1104 1 -0.02689599316459379 1104 2 0.13870314295454733 1104 3 0.041558877747974066 1104 4 -4.964148438588722E-4 1104 5 -0.19173642470838104 1104 6 -0.07170929780271251 1104 7 -0.032404650140712477 1104 8 0.035704335289551636 1104 9 0.05801282452105028 1104 10-11 0.03103660507269268 1104 12-13 0.0517568053660753 1104 14-15 0.09745425712151956 1104 16-17 -0.07247419615623585 1104 18-19 0.02077995612366834 1104 20-21 0.09803153528334008 1104 22-23 0.08200524682838761 1104 24-25 0.043829183801811666 1104 26-27 0.02635365121788169 1104 28-29 -0.04447264829010322 1104 30-31 0.07292378194889437 1104 32-33 0.013621405693555744 1104 34-35 1.7451429079073932E-4 1104 36-37 0.20313386460036753 1104 38-39 0.16303587641161243 1104 40-41 0.04984636722668512 1104 42-43 0.19157413283568303 1104 44-45 0.22655952906342947 1104 46-47 0.34504186430766737 1104 48-49 0.07540239985174679 1104 50-51 -0.09468299047970419 1104 52-53 -0.04882886027508704 1104 54-55 -0.020264922348530945 1104 56-57 0.04908002770852704 1104 58-59 0.08344387635519013 1104 60-61 -0.06297517934698504 1104 62-63 0.11180431301270488 1104 64-65 0.17945418488154985 1104 66-67 0.15821788610319487 1104 68-69 0.17152359845739085 1104 70-71 0.056838637475891574 1104 72-73 0.10309722363489371 1104 74-75 0.2293787550548494 1104 76-77 -0.041771866569035865 1104 78-79 -0.0034533101402836053 1104 80-81 0.16185559973852293 1104 82-83 0.21630959095447722 1104 84-85 0.03289517897650285 1104 86-87 -0.040715503166897093 1104 88-89 0.14447129717036233 1104 90-91 0.14307270160437824 1104 92-93 0.4338497300655533 1104 94-95 0.6469066082467627 1104 96-97 0.7897296310099122 1104 98-99 1.3154449360507208 1104 100-101 2.003682715597904 1105 1 0.2039521200364618 1105 2 0.17216001464671393 1105 3 0.07857737064882286 1105 4 0.12292666446157341 1105 5 0.027053794081837168 1105 6 0.05271344426708424 1105 7 0.13233667636531976 1105 8 0.057194503838545074 1105 9 0.13557701973230252 1105 10-11 0.11296851788694795 1105 12-13 0.08544509640730524 1105 14-15 0.06045210982525617 1105 16-17 0.030950466091958617 1105 18-19 0.16233977415407708 1105 20-21 0.24860294320768617 1105 22-23 0.1910803264917078 1105 24-25 0.28803509712537334 1105 26-27 0.20628520614943824 1105 28-29 -0.02337907572594844 1105 30-31 0.09932881570687613 1105 32-33 0.06387639712501425 1105 34-35 0.22834624981545915 1105 36-37 0.36269363445425284 1105 38-39 0.24554848833599152 1105 40-41 0.2988449838723568 1105 42-43 0.32971944009864274 1105 44-45 0.2766089099951614 1105 46-47 0.19516213354558687 1105 48-49 0.1627404063073996 1105 50-51 0.2297634957314827 1105 52-53 0.1677478195368849 1105 54-55 0.03494794904081289 1105 56-57 0.18681153308708787 1105 58-59 0.26109048782533506 1105 60-61 0.2367232012337368 1105 62-63 0.1551760843256531 1105 64-65 0.1330464325914491 1105 66-67 0.23868643715997706 1105 68-69 0.2142578911916857 1105 70-71 0.3485810101006166 1105 72-73 0.4603717059093775 1105 74-75 0.3065377513314935 1105 76-77 0.25976238349462477 1105 78-79 0.2663782096912364 1105 80-81 0.24210477557593535 1105 82-83 0.2496486022346609 1105 84-85 0.05422963413448656 1105 86-87 0.47759845632353404 1105 88-89 0.6554434993190377 1105 90-91 0.505841887902978 1105 92-93 0.7157976443664111 1105 94-95 0.7958348527632424 1105 96-97 0.7231101085080347 1105 98-99 1.0864550600020237 1105 100-101 1.3104888532863939 1106 1 -0.17737569051278257 1106 2 -0.04643858539779444 1106 3 -0.11115934714654685 1106 4 -0.12735441974266593 1106 5 -0.11708189150986925 1106 6 -0.16763705211980806 1106 7 -0.24598216681702212 1106 8 -0.11397601667474788 1106 9 -0.1857685928165438 1106 10-11 -0.05529990581624844 1106 12-13 -0.16145077751081516 1106 14-15 -0.2262630503732197 1106 16-17 -0.16924004653535718 1106 18-19 -0.0055533826167391 1106 20-21 -0.047224029944871404 1106 22-23 -0.1672571385191688 1106 24-25 -0.13738340602316157 1106 26-27 -0.40686631385244 1106 28-29 -0.2629345527687903 1106 30-31 -0.31543776728519646 1106 32-33 -0.3142228490227694 1106 34-35 -0.6216030964685615 1106 36-37 -0.42148200803787716 1106 38-39 -0.540002807579306 1106 40-41 -0.39739278213284734 1106 42-43 -0.3350822784189518 1106 44-45 -0.40340844305050183 1106 46-47 -0.4548397208293693 1106 48-49 -0.2661496819608473 1106 50-51 -0.3522213491451751 1106 52-53 -0.19118938937504737 1106 54-55 -0.2728495024623996 1106 56-57 -0.49180283890442666 1106 58-59 -0.24742743714860183 1106 60-61 -0.18972594399699716 1106 62-63 -0.10986533655500352 1106 64-65 -0.3504730539614087 1106 66-67 -0.12029810711273115 1106 68-69 -0.42871539409706827 1106 70-71 -0.265020439825399 1106 72-73 -0.034484355554365465 1106 74-75 -0.1738288097984615 1106 76-77 -0.09180124499013687 1106 78-79 0.3420747714848673 1106 80-81 0.4453857504696508 1106 82-83 0.2537706825700887 1106 84-85 0.028670003291740187 1106 86-87 -0.06264928817092397 1106 88-89 -0.043064412030126675 1106 90-91 0.25123035017718465 1106 92-93 0.2021738443422052 1106 94-95 -0.1747123049679402 1106 96-97 0.08897633585050002 1106 98-99 0.11492459554941448 1106 100-101 0.7728552894900389 1107 1 0.11689368916663767 1107 2 0.11068168253969901 1107 3 0.052667303972540935 1107 4 0.05567958297087472 1107 5 -0.1124960841713829 1107 6 0.038445176560706784 1107 7 -0.07674730236502114 1107 8 -0.05581761015588427 1107 9 0.08797440729668438 1107 10-11 -0.032258135934988275 1107 12-13 0.022718006420880954 1107 14-15 0.0055759805385164896 1107 16-17 0.05620249330862492 1107 18-19 0.05199964171178806 1107 20-21 -0.019639162215170813 1107 22-23 0.07601065395640916 1107 24-25 0.07811576859111824 1107 26-27 -0.018118575965544892 1107 28-29 -0.11352384440706231 1107 30-31 -0.047604568701956396 1107 32-33 0.03308128823559997 1107 34-35 0.1731211889420763 1107 36-37 -0.01074186250776421 1107 38-39 -0.03369918482130885 1107 40-41 0.06178389808828655 1107 42-43 -0.030003424781391175 1107 44-45 0.14975047002277364 1107 46-47 0.07695302491408285 1107 48-49 0.15892396707838685 1107 50-51 0.22331584684769012 1107 52-53 0.4332705342246328 1107 54-55 0.3655103736602143 1107 56-57 0.5042217021672073 1107 58-59 0.3755277218598181 1107 60-61 0.5610105463953445 1107 62-63 0.5146467323522899 1107 64-65 0.6067722633899706 1107 66-67 0.3644933414541143 1107 68-69 0.2985552112453931 1107 70-71 0.5176999957716646 1107 72-73 0.3854244249208385 1107 74-75 0.38201980790392653 1107 76-77 0.2350991783304366 1107 78-79 0.3110224891095825 1107 80-81 0.30459292328019316 1107 82-83 0.3761770742935866 1107 84-85 0.27636915367086345 1107 86-87 0.5398713663371133 1107 88-89 0.7229446940304456 1107 90-91 0.6488090200646077 1107 92-93 0.7844025248573416 1107 94-95 0.7943471526016737 1107 96-97 0.8265910803583587 1107 98-99 1.2996560521814011 1107 100-101 1.8762842655945775 1108 1 0.007892557037287418 1108 2 0.04464314992411289 1108 3 -0.028792720600492316 1108 4 -0.03555134125287651 1108 5 -0.2512724192494957 1108 6 -0.01648823291821344 1108 7 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0.3020216001803462 2316 94-95 0.24234917541651413 2316 96-97 -0.18448964875003426 2316 98-99 -0.11829687709615655 2316 100-101 -0.7081268580969713 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 4.0 5 9.0 6 40.0 7 78.0 8 200.0 9 388.0 10 671.0 11 1184.0 12 1961.0 13 2939.0 14 3840.0 15 5712.0 16 8087.0 17 11860.0 18 10476.0 19 5830.0 20 4504.0 21 4455.0 22 4447.0 23 4600.0 24 5251.0 25 6134.0 26 7371.0 27 9319.0 28 11408.0 29 14162.0 30 17760.0 31 22532.0 32 29188.0 33 39473.0 34 54834.0 35 82045.0 36 127229.0 37 192467.0 38 147399.0 39 12373.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58372456964006 19.936931530704697 15.473660677515385 21.005683222139858 2 15.734844760383613 20.898364450257223 40.305962829056476 23.060827960302692 3 19.37393779366881 25.573753138838086 30.328203379065787 24.72410568842732 4 12.694372732244616 17.48622439678442 36.859456503202054 32.95994636776891 5 12.926004695873935 38.6227396989559 33.671604078540604 14.779651526629559 6 31.146330132257553 36.916146712379515 17.025410621769275 14.912112533593652 7 28.13516263150776 34.35403153245867 19.894734985033548 17.616070851000018 8 27.158726704969805 34.7311037536681 19.062376872276488 19.047792669085606 9 27.472757531741227 16.556363828823795 20.26157474110099 35.709303898333985 10-11 25.090945444494757 27.559204220009764 26.202932130528616 21.146918204966862 12-13 26.155270250640584 25.223197089160625 27.459843343019273 21.161689317179516 14-15 22.99046734138209 25.893135427264223 26.681329279552124 24.43506795180156 16-17 21.192787876293025 29.54941869012685 27.486106782242558 21.77168665133757 18-19 21.234776267737743 26.90350314912936 31.309520309091017 20.552200274041883 20-21 22.016279038312373 25.861629938564008 31.786289547177866 20.335801475945754 22-23 21.848716763522468 25.7986928289069 31.646383991211817 20.70620641635881 24-25 21.465683169819044 26.143703214947926 31.801162389302206 20.58945122593082 26-27 21.008067314263 26.530043365195638 31.64259645433364 20.81929286620773 28-29 20.888797147550065 26.609928177117244 31.8481821982989 20.65309247703379 30-31 20.976250039695003 26.37130057290178 31.70942553394187 20.943023853461344 32-33 20.886166765658906 26.29749422218563 31.587972736949197 21.228366275206266 34-35 21.525166571594912 26.412991702294068 31.390203884808315 20.671637841302697 36-37 21.52642684249277 26.11439909483742 31.267039426793097 21.09213463587671 38-39 21.13486271442601 26.56212694137503 31.2840567607779 21.018953583421055 40-41 21.415373396766775 26.483089969243796 31.277881997330148 20.823654636659278 42-43 21.182252972423626 26.520617586584923 31.077471216776374 21.219658224215078 44-45 21.36509318012079 26.78924062171047 30.804365851793914 21.041300346374825 46-47 21.764512164283992 27.24446770598717 29.85680429528307 21.134215834445772 48-49 21.685416384881826 27.502396396290436 29.426687915693893 21.38549930313384 50-51 21.957223591124805 27.61865837092098 29.208395325998104 21.215722711956104 52-53 22.150758319758655 27.59689968067652 29.079607402659263 21.172734596905563 54-55 21.841843725558228 27.78990514387199 29.134356971896004 21.233894158673777 56-57 21.996330426293905 28.13698565690662 28.86948902362288 20.997194893176594 58-59 21.870600481043475 27.45987873940734 29.02279957893994 21.646721200609242 60-61 21.65436614583027 27.698989103012693 29.40622298540992 21.240421765747115 62-63 21.64119331714173 27.62065781813263 29.625221262356877 21.112927602368757 64-65 21.51005310296565 27.59889912788817 29.425335348462482 21.465712420683694 66-67 21.5479249854452 27.958270360547377 28.956935435497243 21.536869218510176 68-69 21.74804612842332 27.72168870959205 29.079783824472056 21.45048133751257 70-71 21.662305127405954 27.84000893870518 29.041323869283197 21.45636206460567 72-73 21.75871583587577 27.798406085854012 28.786369399482965 21.65650867878725 74-75 21.780050092062837 28.25702791406371 28.36335152243293 21.599570471440526 76-77 21.7825659964598 27.983439872505837 28.761930525090122 21.47206360594424 78-79 21.805089181226368 28.004434068228196 28.537757208301233 21.652719542244203 80-81 21.665304298223433 28.54422600810364 28.367804195310708 21.422665498362218 82-83 21.796894617772647 28.469826230088557 28.179553028668007 21.55372612347079 84-85 21.645041247468345 28.391889762112548 28.188945631437935 21.774123358981175 86-87 21.656894858480303 28.29764712109005 28.205907778437727 21.83955024199192 88-89 21.69711903179709 28.15380453639288 28.251306991596444 21.897769440213587 90-91 21.624039243615883 28.30241720395706 28.301299788926183 21.772243763500875 92-93 21.694244037383527 28.00083273152406 28.450837083369603 21.85408614772281 94-95 21.434132916193757 28.001964162849095 28.367921809852568 22.195981111104576 96-97 21.795327174251824 27.92404452886555 28.135574282404274 22.145054014478347 98-99 21.39472356401128 27.97738972728652 28.164397288743203 22.463489419959 100-101 21.322991434291534 27.865254183458138 28.622428934732643 22.189325447517685 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 452.0 1 311.5 2 246.0 3 315.5 4 270.0 5 256.5 6 469.5 7 1022.0 8 1595.5 9 1888.0 10 1844.0 11 1684.0 12 1754.0 13 1859.5 14 2005.0 15 2097.5 16 2099.5 17 2178.5 18 2302.0 19 2419.5 20 2525.0 21 2781.5 22 3171.5 23 3735.5 24 4325.5 25 5164.5 26 6560.5 27 7847.0 28 8627.0 29 9688.5 30 11103.0 31 12767.0 32 15118.5 33 16964.5 34 18520.0 35 20088.5 36 22576.5 37 24825.5 38 25635.0 39 25788.5 40 25692.0 41 27089.5 42 27863.5 43 28231.5 44 29831.0 45 30831.5 46 30265.0 47 30084.0 48 30804.5 49 30284.5 50 29569.0 51 28124.5 52 25817.0 53 25090.0 54 25167.0 55 23293.0 56 20433.5 57 18456.0 58 16908.5 59 15011.0 60 12216.5 61 9953.0 62 8530.0 63 6674.5 64 4907.0 65 3761.5 66 2708.0 67 2083.5 68 1604.0 69 1044.0 70 731.0 71 551.0 72 438.0 73 401.0 74 335.0 75 272.5 76 187.5 77 100.0 78 75.5 79 60.0 80 40.0 81 26.5 82 12.5 83 6.5 84 5.0 85 2.0 86 0.0 87 0.5 88 1.5 89 1.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.042929307779613866 2 1.176145418619558E-4 3 0.0 4 0.0 5 0.014937046816468387 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003940087152375519 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.88072709309779E-5 22-23 2.352290837239116E-4 24-25 4.1165089651684536E-4 26-27 0.0016466035860673814 28-29 0.0021758690244461825 30-31 2.352290837239116E-4 32-33 0.0 34-35 0.0 36-37 1.176145418619558E-4 38-39 0.0 40-41 0.0 42-43 0.010232465141990155 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.176145418619558E-4 74-75 5.88072709309779E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.7642181279293372E-4 84-85 1.176145418619558E-4 86-87 0.0 88-89 0.0 90-91 0.0068804506989244155 92-93 0.002469905379101072 94-95 0.0 96-97 0.0 98-99 2.352290837239116E-4 100-101 0.011879068728057537 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 850235.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.113173287861116 #Duplication Level Percentage of deduplicated Percentage of total 1 78.84594725428107 42.66604406816469 2 11.800260519487006 12.770990846658128 3 3.655531920945645 5.934372968923185 4 1.679763084991638 3.6358924360281875 5 0.8979123614382158 2.429444360590938 6 0.5793297653532142 1.8809623190024642 7 0.3864069088802239 1.463679281390362 8 0.2951351877387236 1.2776561245960778 9 0.22477817639619357 1.0947114369590971 >10 1.4671237168863465 15.020820690076922 >50 0.11202037045853805 4.110430537069972 >100 0.052739677428960366 4.975947664472088 >500 0.0021793255101546236 0.7788111661548396 >1k 4.358651020309247E-4 0.583636653740203 >5k 4.358651020309247E-4 1.376599446172841 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6292 0.740030697395426 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5381 0.6328838497591842 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3573 0.42023675807276817 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1376 0.1618376096020512 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 950 0.11173381476885802 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.88072709309779E-5 0.0 14-15 0.0 0.0 0.0 1.176145418619558E-4 0.0 16-17 0.0 0.0 0.0 1.176145418619558E-4 0.0 18-19 5.88072709309779E-5 0.0 0.0 1.176145418619558E-4 0.0 20-21 1.176145418619558E-4 0.0 0.0 1.176145418619558E-4 0.0 22-23 1.176145418619558E-4 0.0 0.0 1.176145418619558E-4 0.0 24-25 1.176145418619558E-4 0.0 0.0 1.176145418619558E-4 0.0 26-27 1.176145418619558E-4 0.0 0.0 2.352290837239116E-4 0.0 28-29 1.176145418619558E-4 0.0 0.0 9.997236058266244E-4 0.0 30-31 1.176145418619558E-4 0.0 0.0 0.0014113745023434697 0.0 32-33 1.176145418619558E-4 0.0 0.0 0.0018818326697912931 0.0 34-35 2.352290837239116E-4 0.0 0.0 0.002293483566308138 0.0 36-37 4.704581674478232E-4 0.0 0.0 0.0032932071721347624 0.0 38-39 4.704581674478232E-4 0.0 0.0 0.004586967132616276 0.0 40-41 5.292654383788012E-4 0.0 0.0 0.006527607073338547 0.0 42-43 7.644945221027127E-4 0.0 0.0 0.009644392432680376 0.0 44-45 8.233017930336907E-4 0.0 0.0 0.01740695219556946 0.0 46-47 9.997236058266244E-4 0.0 0.0 0.03234399901203785 0.0 48-49 0.0010585308767576023 0.0 0.0 0.04610490040988668 0.0 50-51 0.0010585308767576023 0.0 0.0 0.06374708168918004 0.0 52-53 0.0010585308767576023 0.0 0.0 0.08532935012084894 0.0 54-55 0.0010585308767576023 0.0 0.0 0.10885225849324011 0.0 56-57 0.0010585308767576023 0.0 0.0 0.1368445194563856 0.0 58-59 0.0010585308767576023 0.0 0.0 0.1785388745464489 0.0 60-61 0.0010585308767576023 0.0 0.0 0.23111257475874317 0.0 62-63 0.0010585308767576023 0.0 0.0 0.29768240545261015 0.0 64-65 0.0010585308767576023 0.0 0.0 0.3561368327580022 0.0 66-67 0.0010585308767576023 0.0 0.0 0.44152499014978214 0.0 68-69 0.0010585308767576023 0.0 0.0 0.5420266161708234 0.0 70-71 0.0010585308767576023 0.0 0.0 0.6405876022511423 0.0 72-73 0.0010585308767576023 0.0 0.0 0.769081489235329 0.0 74-75 0.0010585308767576023 0.0 0.0 0.8900480455403506 0.0 76-77 0.0010585308767576023 0.0 0.0 1.0146606526430928 0.0 78-79 0.0010585308767576023 0.0 0.0 1.1651484589554653 0.0 80-81 0.0010585308767576023 0.0 0.0 1.3149893852875971 0.0 82-83 0.0010585308767576023 0.0 0.0 1.5019965068481067 0.0 84-85 0.0010585308767576023 0.0 0.0 1.7221121219427569 0.0 86-87 0.0010585308767576023 0.0 0.0 1.972337059754068 0.0 88-89 0.001176145418619558 0.0 0.0 2.286485501067352 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2805 0.0 55.072094 1 GTATCAA 5305 0.0 43.90276 1 ATCAACG 6935 0.0 33.568066 3 TCAACGC 7120 0.0 32.69586 4 TATCAAC 7125 0.0 32.672916 2 CAACGCA 7235 0.0 32.176163 5 AACGCAG 7370 0.0 31.579346 6 TAAGGTG 425 0.0 27.946522 5 ACGCAGA 8460 0.0 27.456083 7 CGCAGAG 8495 0.0 27.287045 8 CCCTATA 145 7.870367E-7 26.21191 2 GCAGAGT 9045 0.0 25.207672 9 TGGTATC 1250 0.0 24.324652 2 GTACATG 5550 0.0 24.151117 1 TACATGG 5580 0.0 23.669403 2 GAGTACT 5535 0.0 23.385624 12-13 CCTATAC 205 2.6617272E-8 23.175165 3 GTAAGGT 390 0.0 23.145454 4 ACATGGG 5525 0.0 23.13112 3 ACTGTCG 105 0.0021955648 22.619385 8 >>END_MODULE