FastQCFastQC Report
Thu 26 May 2016
SRR937555_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937555_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003746
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82970.41407443857654613No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65800.3283849350167137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43890.21903973856965903No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26620.1328511697590413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39050.058.9456561
GTATCAA73200.044.374871
ATCAACG94500.033.9080923
TCAACGC95300.033.6242944
TATCAAC95950.033.445222
CAACGCA98150.032.504255
AACGCAG99400.032.0188146
GTGGTAT18900.027.1762451
ACGCAGA116400.027.0976687
CGCAGAG117450.026.7738538
TGGTATC18050.026.6023162
GTACATG85500.025.7538621
TACATGG86500.024.8426322
GCAGAGT125250.024.5376249
ACATGGG87950.023.7303413
GAGTACT70500.023.31184412-13
GTACTTT75850.021.72937614-15
CAGAGTA122350.021.35301810-11
AGAGTAC117900.020.66826810-11
AGTACTT75650.020.62605312-13
Produced by FastQC (version 0.11.5)