##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937555_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2003746 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.45699454920933 31.0 31.0 34.0 30.0 34.0 2 31.607045004706187 33.0 31.0 34.0 30.0 34.0 3 31.625945603883924 33.0 31.0 34.0 30.0 34.0 4 35.22241391873022 37.0 35.0 37.0 33.0 37.0 5 35.204763478005695 37.0 35.0 37.0 33.0 37.0 6 35.25615522127056 37.0 35.0 37.0 33.0 37.0 7 35.1809685459135 37.0 35.0 37.0 32.0 37.0 8 35.16406071428215 37.0 35.0 37.0 32.0 37.0 9 36.75389944633701 39.0 37.0 39.0 33.0 39.0 10-11 36.60992461120321 39.0 37.0 39.0 32.0 39.0 12-13 36.4757569073126 39.0 37.0 39.0 32.0 39.0 14-15 37.5818988534475 40.0 37.0 41.0 32.0 41.0 16-17 37.472929453134284 40.0 37.0 41.0 32.0 41.0 18-19 37.38600975373126 40.0 37.0 41.0 32.0 41.0 20-21 37.274343155270174 40.0 37.0 41.0 32.0 41.0 22-23 37.17485774145027 40.0 37.0 41.0 31.0 41.0 24-25 37.205585188941114 40.0 37.0 41.0 31.0 41.0 26-27 37.10789566142615 40.0 37.0 41.0 31.0 41.0 28-29 36.96812120897559 39.5 36.5 41.0 31.0 41.0 30-31 36.7167485300033 39.0 36.0 41.0 30.0 41.0 32-33 36.566546608202835 39.0 36.0 41.0 30.0 41.0 34-35 36.55495506915547 39.0 36.0 41.0 30.0 41.0 36-37 36.39939044170269 39.0 35.5 41.0 30.0 41.0 38-39 36.234677948203014 39.0 35.0 41.0 30.0 41.0 40-41 35.98064150845467 39.0 35.0 41.0 28.5 41.0 42-43 35.69765678883451 39.0 35.0 40.0 27.5 41.0 44-45 35.44149108719368 39.0 35.0 40.0 26.5 41.0 46-47 35.079016502091584 38.0 34.5 40.0 25.5 41.0 48-49 34.7510308192755 38.0 34.0 40.0 24.0 41.0 50-51 33.85369178528616 37.5 33.5 39.5 23.0 40.5 52-53 33.95283508987666 38.0 33.5 39.5 23.0 40.5 54-55 34.20481288546552 38.0 34.0 40.0 23.0 41.0 56-57 33.949498589142536 38.0 33.5 40.0 22.0 41.0 58-59 33.665413430644406 38.0 33.0 40.0 20.5 41.0 60-61 33.31419027162126 37.0 33.0 40.0 18.5 41.0 62-63 32.90691285222778 37.0 32.0 40.0 17.5 41.0 64-65 32.47862902783088 36.0 31.5 39.0 13.5 41.0 66-67 32.01389048312511 35.5 31.0 39.0 9.5 40.0 68-69 31.47612646513081 35.0 31.0 38.5 7.5 40.0 70-71 30.99827498096066 35.0 30.0 38.0 6.0 40.0 72-73 30.50996433679718 35.0 29.5 37.0 2.0 39.5 74-75 29.946584796675825 34.0 29.0 36.5 2.0 39.0 76-77 29.43655208793929 34.0 29.0 36.0 2.0 39.0 78-79 28.857710757750734 34.0 28.0 35.5 2.0 37.5 80-81 28.27355238638031 34.0 26.5 35.0 2.0 37.0 82-83 27.736810204486996 33.0 26.0 35.0 2.0 36.5 84-85 27.201858918246124 33.0 25.5 35.0 2.0 36.0 86-87 26.6239288812055 32.5 25.0 35.0 2.0 36.0 88-89 26.10401343284029 32.0 23.5 35.0 2.0 35.0 90-91 25.50172451997409 32.0 21.0 34.5 2.0 35.0 92-93 24.81113973527583 31.5 18.5 34.0 2.0 35.0 94-95 24.00627923898538 31.0 9.0 34.0 2.0 35.0 96-97 23.12072762715434 31.0 2.0 34.0 2.0 35.0 98-99 22.004920533840117 30.0 2.0 34.0 2.0 35.0 100-101 19.24514459417511 26.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29949302445134407 1101 2 0.383852999189326 1101 3 0.3320030377739762 1101 4 0.2352518435516373 1101 5 0.22928906745632816 1101 6 0.3204885584412196 1101 7 0.24011398010908636 1101 8 0.27997786467310704 1101 9 0.3513395733393381 1101 10-11 0.32412930168232634 1101 12-13 0.31268970837562193 1101 14-15 0.5128546852298896 1101 16-17 0.40748173475611793 1101 18-19 0.5656113980433091 1101 20-21 0.5052445598651545 1101 22-23 0.4410594305579423 1101 24-25 0.41580235369342233 1101 26-27 0.3897158710639985 1101 28-29 0.48683334844919557 1101 30-31 0.47587393091863106 1101 32-33 0.5763828818670831 1101 34-35 0.6423864248638367 1101 36-37 0.8092271940406448 1101 38-39 0.4646962106068102 1101 40-41 0.6723692125379941 1101 42-43 0.6672569938052817 1101 44-45 0.648047757218869 1101 46-47 0.5717901853500251 1101 48-49 0.9718781180469378 1101 50-51 0.9282060687252027 1101 52-53 0.8087896564365238 1101 54-55 0.832927785712485 1101 56-57 0.7581662448165432 1101 58-59 0.6551506694170683 1101 60-61 0.9957547007377698 1101 62-63 1.0584669987095339 1101 64-65 1.0325849298627858 1101 66-67 1.034749504087916 1101 68-69 0.9972273635644413 1101 70-71 1.1309237510536967 1101 72-73 1.0333379768033915 1101 74-75 1.024633307339517 1101 76-77 0.9914031423738656 1101 78-79 1.250231015473311 1101 80-81 1.2822533484622873 1101 82-83 1.2923706059373998 1101 84-85 1.2377481041605982 1101 86-87 1.289389649194515 1101 88-89 1.2488970665720416 1101 90-91 1.3226870368474586 1101 92-93 1.288301305480271 1101 94-95 1.3398297318849224 1101 96-97 1.537064409567492 1101 98-99 1.7042575088785519 1101 100-101 1.852650194929815 1102 1 0.1537954038970355 1102 2 0.371908206630831 1102 3 0.3472095900386485 1102 4 0.3435216891544357 1102 5 0.19012571993648208 1102 6 0.2998850320803683 1102 7 0.35712889869176223 1102 8 0.24522918922983195 1102 9 0.3534018905794625 1102 10-11 0.3366102243430973 1102 12-13 0.35627729249169704 1102 14-15 0.44407131281127477 1102 16-17 0.6508279310759022 1102 18-19 0.49508443953988746 1102 20-21 0.5777891150167633 1102 22-23 0.5209653053444185 1102 24-25 0.5749414848834817 1102 26-27 0.3942877114636971 1102 28-29 0.39107880607785717 1102 30-31 0.5394283545865264 1102 32-33 0.5646283456997665 1102 34-35 0.7035029300193685 1102 36-37 0.7808872637737494 1102 38-39 0.6666970564205528 1102 40-41 0.572462634630071 1102 42-43 0.7667024067650487 1102 44-45 0.7605528755293918 1102 46-47 0.8852011412229501 1102 48-49 0.8786822139361519 1102 50-51 0.8047219158417818 1102 52-53 0.8145637828081291 1102 54-55 0.681864469626305 1102 56-57 0.8191966692950885 1102 58-59 0.7242654395170334 1102 60-61 0.718800591820127 1102 62-63 0.8562404819318132 1102 64-65 0.8531170583768457 1102 66-67 0.6960773592779894 1102 68-69 0.7394956126679588 1102 70-71 0.6869702852853692 1102 72-73 0.7135622208444374 1102 74-75 0.8789592103335728 1102 76-77 0.9389794930908444 1102 78-79 1.1005855748022846 1102 80-81 0.8927188975794564 1102 82-83 1.108463246323872 1102 84-85 1.1965873230340343 1102 86-87 1.3165642590655118 1102 88-89 1.3078907653630871 1102 90-91 1.2025832954141684 1102 92-93 1.358875569580956 1102 94-95 1.6012133197895757 1102 96-97 1.8770060504875055 1102 98-99 1.7478249299531683 1102 100-101 1.8926265266211537 1103 1 0.23246079717747392 1103 2 0.284070024634552 1103 3 0.22710037268141292 1103 4 0.24042980944045667 1103 5 0.24219203397311873 1103 6 0.16647333651763319 1103 7 0.33223223888178666 1103 8 0.243395513061742 1103 9 0.23642912145417938 1103 10-11 0.25990584585353105 1103 12-13 0.28580442541362316 1103 14-15 0.39935439636500547 1103 16-17 0.38044297662395365 1103 18-19 0.30435395663264586 1103 20-21 0.317370856617309 1103 22-23 0.3381680186366225 1103 24-25 0.45736875493930995 1103 26-27 0.42015067551502483 1103 28-29 0.29806994135066134 1103 30-31 0.4347114381402548 1103 32-33 0.5834480146193428 1103 34-35 0.5201948195286263 1103 36-37 0.5501643722074903 1103 38-39 0.5399708834359274 1103 40-41 0.699408221857027 1103 42-43 0.5781866363663823 1103 44-45 0.560251670710116 1103 46-47 0.6927168136688309 1103 48-49 0.6421240299808275 1103 50-51 0.5476218080937159 1103 52-53 0.6044406071787805 1103 54-55 0.6404189313757414 1103 56-57 0.5566915267737897 1103 58-59 0.5679514028826986 1103 60-61 0.8189761721903892 1103 62-63 0.8181040336998606 1103 64-65 0.9628288323018097 1103 66-67 1.140100035348123 1103 68-69 1.1155358712633188 1103 70-71 1.0596713333801908 1103 72-73 0.9076182008620712 1103 74-75 0.9048797216471236 1103 76-77 0.8867473939626223 1103 78-79 0.9234786103591439 1103 80-81 0.9378349716317658 1103 82-83 1.1511111550318702 1103 84-85 1.2379025840782099 1103 86-87 1.2471480576745826 1103 88-89 1.3667670020170206 1103 90-91 1.366534465949968 1103 92-93 1.143638523837005 1103 94-95 1.0684738252259542 1103 96-97 1.3635101155281575 1103 98-99 1.3885201498571753 1103 100-101 1.555551151951743 1104 1 0.19073234347619206 1104 2 0.19469106151678162 1104 3 0.20694600404912222 1104 4 0.14214021388627884 1104 5 0.10888498288897352 1104 6 0.08774533404773166 1104 7 0.19102436370663867 1104 8 0.06538615594772779 1104 9 0.11961718994635362 1104 10-11 0.16767890021503717 1104 12-13 0.27800192226688836 1104 14-15 0.2677247385883845 1104 16-17 0.2424915671195933 1104 18-19 0.1822730674693247 1104 20-21 0.2996844861800909 1104 22-23 0.2371579728010076 1104 24-25 0.19686931304129018 1104 26-27 0.2443799010594745 1104 28-29 0.2416023706379491 1104 30-31 0.29370495465769864 1104 32-33 0.2085105932879756 1104 34-35 0.3316594216137929 1104 36-37 0.35113808204195607 1104 38-39 0.4647370743305501 1104 40-41 0.5311369130485133 1104 42-43 0.19915250902413817 1104 44-45 0.34506179729239506 1104 46-47 0.4677418688853052 1104 48-49 0.20466897500183734 1104 50-51 0.2763968336239131 1104 52-53 0.3159097215778175 1104 54-55 0.43210178695795065 1104 56-57 0.26456480200118193 1104 58-59 0.45088369442567 1104 60-61 0.515885474681653 1104 62-63 0.48931855860070783 1104 64-65 0.6511341022094257 1104 66-67 0.8579421993588561 1104 68-69 0.7587659755215128 1104 70-71 0.6633016086313326 1104 72-73 0.8201709830598034 1104 74-75 0.7735089281923528 1104 76-77 0.6439647212478725 1104 78-79 0.6182106026644618 1104 80-81 0.9336000463224359 1104 82-83 0.9194254495672212 1104 84-85 1.0175520485378016 1104 86-87 0.99799494774037 1104 88-89 0.9791476038171965 1104 90-91 0.8338007162171444 1104 92-93 0.8799897243499828 1104 94-95 0.980849439731859 1104 96-97 0.9768924510068189 1104 98-99 1.0707722694572404 1104 100-101 1.149406567915836 1105 1 0.22301786704425552 1105 2 0.32120903840495174 1105 3 0.26655970457516887 1105 4 0.26606163121586235 1105 5 0.33038559834707826 1105 6 0.30423042526413724 1105 7 0.35706332434794064 1105 8 0.3218025363192041 1105 9 0.4025105166658065 1105 10-11 0.4705516504247811 1105 12-13 0.3865001432561499 1105 14-15 0.4405077115265996 1105 16-17 0.3991914868957025 1105 18-19 0.3920631380785977 1105 20-21 0.44756283981435985 1105 22-23 0.5931790514755306 1105 24-25 0.45983373895055024 1105 26-27 0.47523486728582753 1105 28-29 0.4073182923247316 1105 30-31 0.5739634470609261 1105 32-33 0.41695492341847284 1105 34-35 0.6628799883251588 1105 36-37 0.5471890456472579 1105 38-39 0.5724437733317345 1105 40-41 0.5488642497990242 1105 42-43 0.6878124638293031 1105 44-45 0.7257088550352222 1105 46-47 0.4785567844052565 1105 48-49 0.5743862929517789 1105 50-51 0.699879469131865 1105 52-53 0.7154201108780285 1105 54-55 0.6392677401831364 1105 56-57 0.8084475771354533 1105 58-59 0.7766753695127804 1105 60-61 0.9054153381785639 1105 62-63 0.8326140458536813 1105 64-65 0.8891403241540274 1105 66-67 1.018500214705501 1105 68-69 0.93566271941134 1105 70-71 0.8902908243356187 1105 72-73 0.843336397376401 1105 74-75 0.8063425462898799 1105 76-77 0.8086166046721459 1105 78-79 0.7515690442388099 1105 80-81 0.8541538973754506 1105 82-83 0.9586218633755301 1105 84-85 0.8337903441169132 1105 86-87 0.8062399429330576 1105 88-89 0.7881029785117626 1105 90-91 0.7060365132200808 1105 92-93 0.761189469593198 1105 94-95 0.7862207661206178 1105 96-97 1.0004941975605561 1105 98-99 1.2443957560733239 1105 100-101 1.3024964125509406 1106 1 0.13117100800839765 1106 2 0.23443308180450728 1106 3 0.11512021559170904 1106 4 0.18148420722272363 1106 5 0.04525260834755329 1106 6 0.15340279205346263 1106 7 0.20533828659231546 1106 8 0.2877342043050888 1106 9 0.29338265110911266 1106 10-11 0.10700236118558593 1106 12-13 -0.09590556656166171 1106 14-15 -0.04903020592568197 1106 16-17 -0.0799886553095206 1106 18-19 0.09961597545423473 1106 20-21 0.06243858027560378 1106 22-23 0.07880659072522889 1106 24-25 -0.0011435131345223226 1106 26-27 0.058418311631072584 1106 28-29 0.19878195166118928 1106 30-31 0.12658605818256774 1106 32-33 0.19104327761085216 1106 34-35 0.4091623073091881 1106 36-37 0.19936676502902628 1106 38-39 0.2195983425220831 1106 40-41 0.275640500630459 1106 42-43 0.34458597493604515 1106 44-45 0.1066125879583737 1106 46-47 0.38617838018878103 1106 48-49 0.293848612331324 1106 50-51 0.2859354038916848 1106 52-53 0.17376604549797037 1106 54-55 0.29406676835261436 1106 56-57 0.3925894427818619 1106 58-59 0.2097897901598884 1106 60-61 0.1878850552971656 1106 62-63 0.1982530986999862 1106 64-65 0.31274445129847805 1106 66-67 0.27772423591714457 1106 68-69 0.07932401901518205 1106 70-71 -0.09358013173665469 1106 72-73 0.22281383347641892 1106 74-75 0.19809592669052734 1106 76-77 0.2855169389860883 1106 78-79 0.2536009263380379 1106 80-81 0.2564710147220062 1106 82-83 0.08316839793729969 1106 84-85 0.15918872565348252 1106 86-87 0.3226043716594589 1106 88-89 0.4541910712726711 1106 90-91 0.46154261224279836 1106 92-93 0.3733897579395773 1106 94-95 0.74957320999847 1106 96-97 1.08346999199631 1106 98-99 1.346244780842266 1106 100-101 1.5320869105296673 1107 1 0.24606216546781567 1107 2 0.3365795722084144 1107 3 0.16217405886496294 1107 4 0.14624410620014316 1107 5 0.23618748862507033 1107 6 0.1560705811932479 1107 7 0.14619582923815244 1107 8 0.28563866575263575 1107 9 0.20595185990922715 1107 10-11 0.29281978882581683 1107 12-13 0.2967502795407526 1107 14-15 0.20197757764004365 1107 16-17 0.32505509911405284 1107 18-19 0.4510719341870626 1107 20-21 0.39992492048648387 1107 22-23 0.2921559758072547 1107 24-25 0.302696886894978 1107 26-27 0.3811183191972276 1107 28-29 0.3581683457929188 1107 30-31 0.499544033440273 1107 32-33 0.47827428391949667 1107 34-35 0.4082766357934702 1107 36-37 0.4320567833506175 1107 38-39 0.37943200196642124 1107 40-41 0.42441583648648873 1107 42-43 0.3410162326738728 1107 44-45 0.3554005660030981 1107 46-47 0.5376792091141169 1107 48-49 0.5303269774281674 1107 50-51 0.31526408451647825 1107 52-53 0.3063428587661434 1107 54-55 0.40719735737325635 1107 56-57 0.4079276740055775 1107 58-59 0.40649290847066055 1107 60-61 0.5752747593837526 1107 62-63 0.702300514569572 1107 64-65 0.7129403573363717 1107 66-67 0.7880743096153644 1107 68-69 0.8194537738429233 1107 70-71 0.7173815557273677 1107 72-73 0.7025592753448571 1107 74-75 0.6970579742653804 1107 76-77 0.7263704112302598 1107 78-79 0.7728235179292611 1107 80-81 0.935631180717774 1107 82-83 0.8609769032570753 1107 84-85 0.9320428338380573 1107 86-87 0.7555131381553615 1107 88-89 0.7726930581075528 1107 90-91 1.178551288423666 1107 92-93 1.2972038104985195 1107 94-95 1.6477995205002038 1107 96-97 1.6284469726777075 1107 98-99 1.574527272464426 1107 100-101 1.6764599872648809 1108 1 0.03931908502380921 1108 2 0.08485631635016233 1108 3 0.16631177782662832 1108 4 0.14077159529458072 1108 5 0.14737824277571576 1108 6 0.1881804232768829 1108 7 0.22944820424257273 1108 8 0.018782325286807122 1108 9 0.29505092296964364 1108 10-11 0.3553352661533964 1108 12-13 0.36821811527290293 1108 14-15 0.45589623288818615 1108 16-17 0.4045282848991292 1108 18-19 0.4585935752328041 1108 20-21 0.4280541980116297 1108 22-23 0.4795425320984634 1108 24-25 0.499805245876459 1108 26-27 0.4791384458459831 1108 28-29 0.5319062802806229 1108 30-31 0.5965790537002675 1108 32-33 0.46231256700423984 1108 34-35 0.21765514795627183 1108 36-37 0.4606459586097529 1108 38-39 0.4094165178356164 1108 40-41 0.476227126023403 1108 42-43 0.38172126406393403 1108 44-45 0.5208276858187091 1108 46-47 0.5226624595842111 1108 48-49 0.6612195872253395 1108 50-51 0.5749430269149656 1108 52-53 0.44442103049576787 1108 54-55 0.6104292955020512 1108 56-57 0.4164738801214014 1108 58-59 0.4821297579806938 1108 60-61 0.592431802214108 1108 62-63 0.5055302756676596 1108 64-65 0.6756444199477727 1108 66-67 0.825469574029345 1108 68-69 0.7688922359884387 1108 70-71 0.5313719713497385 1108 72-73 0.570988384422261 1108 74-75 0.6070962627927052 1108 76-77 0.36739641858317995 1108 78-79 0.5158542984219743 1108 80-81 0.520282978264806 1108 82-83 0.6383625887360118 1108 84-85 0.6424351619818935 1108 86-87 0.6310346050386393 1108 88-89 0.7346167912797874 1108 90-91 0.8792837231875836 1108 92-93 1.1098383441921484 1108 94-95 1.2912461138553581 1108 96-97 1.2426812157377398 1108 98-99 1.3133924407897162 1108 100-101 1.2978757103248064 1109 1 0.08864802645043923 1109 2 0.11319984518120307 1109 3 0.13704701537426445 1109 4 0.16699917977753387 1109 5 0.12698204903458787 1109 6 0.01728887435228188 1109 7 0.0996331425700987 1109 8 0.058663875798025344 1109 9 0.16877275652289114 1109 10-11 0.15603656541826894 1109 12-13 0.21519320987112422 1109 14-15 0.32146720562138853 1109 16-17 0.3171668146138842 1109 18-19 0.3195033454171039 1109 20-21 0.2617480238930554 1109 22-23 0.26911455624943414 1109 24-25 0.14620692000837465 1109 26-27 0.26370097966335493 1109 28-29 0.12856405103086388 1109 30-31 0.17927039387821253 1109 32-33 0.24019321888325607 1109 34-35 0.22438144167249163 1109 36-37 0.32993938417493496 1109 38-39 0.41117965411626045 1109 40-41 0.3841208279586752 1109 42-43 0.43117756445661115 1109 44-45 0.2802133748747906 1109 46-47 0.12847041266006443 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-0.5311320174731442 2316 76-77 -0.6521214880147888 2316 78-79 -0.6734458336695717 2316 80-81 -0.7051596732715417 2316 82-83 -0.6705292817943587 2316 84-85 -0.8222124686542891 2316 86-87 -1.008529981752023 2316 88-89 -1.0277834270922561 2316 90-91 -0.8514023018165595 2316 92-93 -1.2611130137071314 2316 94-95 -1.2272233896560678 2316 96-97 -1.1518370303689984 2316 98-99 -1.0440720443293863 2316 100-101 -1.2100235270932238 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11233.0 3 2822.0 4 2587.0 5 2815.0 6 3083.0 7 3631.0 8 4295.0 9 5091.0 10 6316.0 11 7644.0 12 9309.0 13 11217.0 14 12909.0 15 15164.0 16 18451.0 17 22552.0 18 20011.0 19 15951.0 20 16554.0 21 17614.0 22 19169.0 23 21368.0 24 23798.0 25 27137.0 26 30482.0 27 35619.0 28 41637.0 29 49735.0 30 59544.0 31 72266.0 32 89755.0 33 114583.0 34 150870.0 35 203572.0 36 277136.0 37 339388.0 38 218956.0 39 19480.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.262875423673655 18.868935271176174 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26.879306607583903 29.202865233949687 21.73994134225775 20-21 22.868111146137988 26.00600534536236 29.378168512160812 21.747714996338843 22-23 22.650600722932467 25.89992192689327 29.268615236080525 22.18086211409373 24-25 22.30124819029033 26.162345488732235 29.466085959553006 22.07032036142443 26-27 22.139699553487613 26.543950133408735 29.20005009858471 22.11630021451894 28-29 22.00207520752075 26.425142514251426 29.496049604960493 22.076732673267326 30-31 22.19655185260474 26.208305548075312 29.382013355426523 22.21312924389343 32-33 21.939971250090952 26.344998408478247 29.335959115018888 22.37907122641191 34-35 22.452619448098492 26.403563758619164 29.07175699790942 22.072059795372926 36-37 22.374432648073945 26.26293532394273 29.032245162525687 22.330386865457633 38-39 22.18645024844719 26.45638245194717 29.10725234830649 22.24991495129915 40-41 22.350803744223704 26.306331213713026 29.17549286296727 22.167372179096002 42-43 22.30702962267415 26.275885965254382 28.938431677251554 22.478652734819917 44-45 22.293908476770426 26.53503130252558 28.784097951991544 22.38696226871245 46-47 22.37343588738389 26.741600395228453 28.30668808154187 22.57827563584578 48-49 22.57971042030858 26.82247392822712 28.132663021085435 22.465152630378864 50-51 22.506978260630408 26.983591211442047 27.968848604497893 22.540581923429652 52-53 22.67885982255437 26.960477109573848 27.896645598395096 22.46401746947668 54-55 22.53334373293815 26.857054431931587 27.974836956195663 22.6347648789346 56-57 22.462727885128523 27.26602835292809 27.933020800395937 22.338222961547448 58-59 22.61669917822902 26.856683500690394 28.002028681497006 22.524588639583584 60-61 22.64082892976062 26.740463063726878 28.302079672715113 22.316628333797397 62-63 22.51746972231415 26.82835929087275 28.201640939090684 22.452530047722412 64-65 22.43740628345457 26.78460422904542 28.17221523605134 22.60577425144866 66-67 22.30931582751867 27.096278647991756 27.959030234922782 22.6353752895668 68-69 22.2838413221934 26.839080158074864 28.14310529766775 22.733973222063987 70-71 22.357034053068535 26.94087792997017 28.036678149835105 22.665409867126193 72-73 22.52720376422363 26.876781663781845 27.914342666824965 22.68167190516956 74-75 22.3322314908968 27.0639298580303 27.79255066525487 22.811287985818026 76-77 22.465296560302445 27.039511377168875 27.77492533373595 22.720266728792733 78-79 22.5507765755681 27.04591808803248 27.673992999058328 22.7293123373411 80-81 22.317011687266085 27.35413163615476 27.696323549077544 22.632533127501606 82-83 22.515562609744364 27.2936118627016 27.615749448544346 22.575076079009694 84-85 22.522042231906575 27.042536833696744 27.619348975594832 22.816071958801846 86-87 22.507534219527837 26.93896494894626 27.79463434604374 22.758866485482162 88-89 22.586180221445783 26.87650169749773 27.64480594187942 22.892512139177068 90-91 22.55172703403676 27.052433925175524 27.685486435088997 22.710352605698723 92-93 22.655527134925567 26.827627851595327 27.75057073146104 22.766274282018074 94-95 22.547359633270595 26.825036180535243 27.656001219765198 22.97160296642896 96-97 22.608112076884144 26.819793417607617 27.54886804596552 23.02322645954272 98-99 22.5508866816657 26.720753827514816 27.482020034978856 23.246339455840637 100-101 22.92333132659439 26.109797859161915 27.928058283895833 23.038812530347865 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2489.0 1 1433.5 2 431.5 3 517.0 4 453.0 5 380.5 6 652.5 7 1477.5 8 2533.5 9 2968.5 10 2490.0 11 1987.0 12 2008.0 13 2228.0 14 2411.0 15 2530.5 16 2719.5 17 2864.5 18 2900.0 19 3101.0 20 3542.5 21 4149.0 22 4897.5 23 5762.0 24 6946.5 25 8622.5 26 10828.5 27 13323.0 28 15540.0 29 18213.5 30 21502.5 31 24949.5 32 28551.5 33 32439.5 34 36927.5 35 40691.5 36 44944.5 37 49880.0 38 53262.5 39 55314.5 40 57406.5 41 61297.5 42 64716.5 43 67817.5 44 71898.0 45 75304.0 46 76741.5 47 76963.5 48 78530.0 49 78492.5 50 75990.5 51 73031.5 52 69925.0 53 68565.5 54 67212.5 55 62974.0 56 57504.0 57 51890.0 58 46622.5 59 41053.5 60 34636.0 61 28189.5 62 22542.0 63 17902.0 64 14078.5 65 11060.0 66 8490.5 67 6766.5 68 5138.5 69 3457.5 70 2404.0 71 1803.0 72 1474.0 73 1171.0 74 869.0 75 677.5 76 470.5 77 312.0 78 216.5 79 160.0 80 103.5 81 64.5 82 53.5 83 41.5 84 25.5 85 17.5 86 15.5 87 9.0 88 6.5 89 8.0 90 7.0 91 6.0 92 4.0 93 1.5 94 1.0 95 2.0 96 2.0 97 0.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3162077428975529 2 0.2789774751889711 3 0.278877662138814 4 0.28112345576734776 5 0.2881602758034202 6 0.1952343261072012 7 0.19388684993008096 8 0.19493488695673003 9 0.19418628908055213 10-11 0.20162236131725275 12-13 0.1920652617647147 14-15 0.1926142335405785 16-17 0.19865292307508037 18-19 0.19161610303900795 20-21 0.1843796569026214 22-23 0.18452937647785697 24-25 0.18817255280858952 26-27 0.18413012427722875 28-29 0.19693114795987116 30-31 0.2013478754293209 32-33 0.20399292125848284 34-35 0.20259553855628407 36-37 0.1773927433916275 38-39 0.17075517555618328 40-41 0.17691863140338146 42-43 0.18585189939243796 44-45 0.1910172247380656 46-47 0.19413638255547358 48-49 0.19373713035484538 50-51 0.19782946541128466 52-53 0.2182412341684026 54-55 0.18315694703819746 56-57 0.17085498860634032 58-59 0.1712791940695078 60-61 0.16404274793312126 62-63 0.1710795679691937 64-65 0.1679604101517857 66-67 0.16688741986259736 68-69 0.1773927433916275 70-71 0.17102966144411516 72-73 0.16636340134927283 74-75 0.1726765767717066 76-77 0.17527171607579003 78-79 0.17898975219414037 80-81 0.17000657768000535 82-83 0.167611064476236 84-85 0.16309452395662924 86-87 0.16005022592683904 88-89 0.16476639254675993 90-91 0.171578633219979 92-93 0.16184686082966604 94-95 0.16773583078893234 96-97 0.16636340134927283 98-99 0.16940769937906303 100-101 0.14909574367210215 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2003746.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.428130008107644 #Duplication Level Percentage of deduplicated Percentage of total 1 76.29879694075424 42.29099636294332 2 13.207527927168329 14.641371500655973 3 4.417438142458861 7.3455100698894995 4 2.0080235330628846 4.452039577997969 5 1.0534646639283383 2.919578817558368 6 0.6542472474258996 2.1758220892661595 7 0.4417686120338103 1.7140485642917913 8 0.30816902873267565 1.3664986391253606 9 0.2356040431929728 1.1753182382882315 >10 1.2373886769050482 12.501928599834239 >50 0.09386748570343005 3.605786708454147 >100 0.041722124758103246 4.046003511180995 >500 0.0013510730968294353 0.4829242364964454 >1k 4.50357698943142E-4 0.5394393027338016 >5k 1.8014307957725683E-4 0.7427337812837359 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8297 0.41407443857654613 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6580 0.3283849350167137 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4389 0.21903973856965903 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2662 0.1328511697590413 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.495326253926396E-5 0.0 22-23 0.0 0.0 0.0 4.990652507852792E-5 0.0 24-25 0.0 0.0 0.0 4.990652507852792E-5 0.0 26-27 0.0 0.0 0.0 9.981305015705584E-5 0.0 28-29 0.0 0.0 0.0 3.9925220062822335E-4 0.0 30-31 0.0 0.0 0.0 0.001172803339345406 0.0 32-33 0.0 0.0 0.0 0.00227074689107302 0.0 34-35 0.0 0.0 0.0 0.003568316543114746 0.0 36-37 0.0 0.0 0.0 0.005888969959266294 0.0 38-39 0.0 0.0 0.0 0.007935137487485939 0.0 40-41 0.0 0.0 0.0 0.010006258278244848 0.0 42-43 0.0 0.0 0.0 0.011902706231228908 0.0 44-45 0.0 0.0 0.0 0.013774200921673706 0.0 46-47 0.0 0.0 0.0 0.017392423989866977 0.0 48-49 0.0 0.0 0.0 0.022532796072955354 0.0 50-51 0.0 0.0 0.0 0.0273487757430333 0.0 52-53 0.0 0.0 0.0 0.03610737089431495 0.0 54-55 0.0 0.0 0.0 0.04953222614043896 4.990652507852792E-5 56-57 0.0 0.0 0.0 0.07366203101590721 4.990652507852792E-5 58-59 0.0 0.0 0.0 0.10091099370878345 4.990652507852792E-5 60-61 0.0 0.0 0.0 0.12743631178802103 4.990652507852792E-5 62-63 0.0 0.0 0.0 0.15870274974971876 4.990652507852792E-5 64-65 0.0 0.0 0.0 0.19857806328746258 4.990652507852792E-5 66-67 0.0 0.0 0.0 0.2551970159890525 9.981305015705584E-5 68-69 0.0 0.0 0.0 0.3192520409273431 9.981305015705584E-5 70-71 0.0 0.0 0.0 0.3861018312700312 9.981305015705584E-5 72-73 0.0 0.0 0.0 0.4582167600085041 9.981305015705584E-5 74-75 0.0 0.0 0.0 0.5369692565824211 9.981305015705584E-5 76-77 0.0 0.0 0.0 0.631392402030996 9.981305015705584E-5 78-79 0.0 0.0 0.0 0.725466201804021 9.981305015705584E-5 80-81 0.0 0.0 0.0 0.8279242977902388 9.981305015705584E-5 82-83 0.0 0.0 0.0 0.9447305197365334 9.981305015705584E-5 84-85 0.0 0.0 0.0 1.0777064558082712 9.981305015705584E-5 86-87 0.0 0.0 0.0 1.2252301439403996 9.981305015705584E-5 88-89 0.0 0.0 0.0 1.40828727792844 9.981305015705584E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3905 0.0 58.945656 1 GTATCAA 7320 0.0 44.37487 1 ATCAACG 9450 0.0 33.908092 3 TCAACGC 9530 0.0 33.624294 4 TATCAAC 9595 0.0 33.44522 2 CAACGCA 9815 0.0 32.50425 5 AACGCAG 9940 0.0 32.018814 6 GTGGTAT 1890 0.0 27.176245 1 ACGCAGA 11640 0.0 27.097668 7 CGCAGAG 11745 0.0 26.773853 8 TGGTATC 1805 0.0 26.602316 2 GTACATG 8550 0.0 25.753862 1 TACATGG 8650 0.0 24.842632 2 GCAGAGT 12525 0.0 24.537624 9 ACATGGG 8795 0.0 23.730341 3 GAGTACT 7050 0.0 23.311844 12-13 GTACTTT 7585 0.0 21.729376 14-15 CAGAGTA 12235 0.0 21.353018 10-11 AGAGTAC 11790 0.0 20.668268 10-11 AGTACTT 7565 0.0 20.626053 12-13 >>END_MODULE