FastQCFastQC Report
Thu 26 May 2016
SRR937555_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937555_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003746
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92220.4602379742741845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78380.3911673435655018No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53170.2653529938425329No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20170.1006614610833908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44700.054.647061
GTATCAA79150.042.210031
ATCAACG102850.032.1997833
TCAACGC103850.031.7982184
TATCAAC104750.031.706452
CAACGCA107100.030.7881535
AACGCAG109800.030.0213286
ACGCAGA126000.026.087357
CGCAGAG128000.025.5312988
GCAGAGT137400.023.4734789
GAGTACT80550.022.49693912-13
GTACATG85600.022.4849781
TTACGCT1503.0276116E-522.173354
GTGGTAT23400.021.9340631
GTACTTT84600.021.50310314-15
TACATGG87450.021.4615822
TGGTATC22600.021.0240352
CGTAGCA1604.9915914E-520.7812887
AGAGTAC127550.020.63116310-11
CAGAGTA134000.020.5597410-11
Produced by FastQC (version 0.11.5)